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The map label for this gene is prfC [H]

Identifier: 57634622

GI number: 57634622

Start: 1070500

End: 1070718

Strand: Direct

Name: prfC [H]

Synonym: SAV1020

Alternate gene names: 57634622

Gene position: 1070500-1070718 (Clockwise)

Preceding gene: 15924009

Following gene: 57634623

Centisome position: 37.19

GC content: 39.73

Gene sequence:

>219_bases
ATGAACTTAAAGCAAGAAGTTGAGTCTAGAAAGACTTTTGCGATTATTTCACATCCCGATGCAGGGAAAACAACGTTAAC
TGAAAAACTATTGTACTTCAGTGGTGCTATTCGTGAAGCGGGTACAGTTAAAGGGAAGAAGACTGGTAAATTTGGCGACC
AAGTGACTGGGAATGAAAGTTTGAACCAAAGAGGCGTTGGTTATTTCCTGTATACTTAG

Upstream 100 bases:

>100_bases
TACTCATGCAATTGTTTGTTTTTAAAGAAGAGGAAGAGGCAGAATCGATTGCATCACAACTAACAGATTGGATAGAAACA
TATAAAAAGGAGAAAGACTA

Downstream 100 bases:

>100_bases
TTCCTAGTAATTGCAATGTTGATTTACGGATGATGTATAAAAATCAAATATCTTTTAGATTACACCCAGGGACACTGAAG
ACCTTTGTCAGATAGAATAC

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 72; Mature: 72

Protein sequence:

>72_residues
MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVKGKKTGKFGDQVTGNESLNQRGVGYFLYT

Sequences:

>Translated_72_residues
MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVKGKKTGKFGDQVTGNESLNQRGVGYFLYT
>Mature_72_residues
MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVKGKKTGKFGDQVTGNESLNQRGVGYFLYT

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790835, Length=62, Percent_Identity=56.4516129032258, Blast_Score=67, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 7928; Mature: 7928

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVKGKKTGKFGDQVTGNES
CCCHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
LNQRGVGYFLYT
CCCCCCEEEEEC
>Mature Secondary Structure
MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVKGKKTGKFGDQVTGNES
CCCHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
LNQRGVGYFLYT
CCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA