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The map label for this gene is gutB [H]

Identifier: 15899935

GI number: 15899935

Start: 2980572

End: 2981609

Strand: Reverse

Name: gutB [H]

Synonym: SSO3237

Alternate gene names: 15899935

Gene position: 2981609-2980572 (Counterclockwise)

Preceding gene: 15899940

Following gene: 15899932

Centisome position: 99.64

GC content: 39.4

Gene sequence:

>1038_bases
ATGGTGGAGGGATTCTCACCTATGAAAAGCGTAATTATGGAGAACGGAAAGGCTGTAATAAAGGAGCTACCATTACCTAA
ATTACAGCAAGGTGATGTATTAGTTAAAATGAAGGCTTGTGGATTGTGCGGTACTGACATCGAGAAGATTTGTGGGCAAT
ACACCGCATCACAACCAATATTGGGTCATGAACCGACTGGAATAATCCAAGAATCTACAGTGGATTGGCTAAAGCCTGGG
GATAGGGTCTTTGCTCATCACCATGTCCCATGCTATGAGTGTTATTATTGTAAAAAGGGAAGCCCGACCATGTGTCCTTA
TTATAGAAAAACTAACTTAGATCCCGGTGGATTTTCGGAGTACTTCAGAGTCCCAGCTTGGAATGTTGCAAGAGGGGGAG
TATTAAAGTTGCCAGATAATGTAAGTTTCGAAGAAGGGGCATTCATAGAACCATTAGCTACCGTTATTAGGGCGCAAAAG
AGAGTAGCTATCGATAATGGCGATACGGTCTTTATTGTTGGAATTGGTCCCATGGGACTATTACACGCAATGATGGCTAA
GGTAAATAAGGCAGGAGTTATCATAGCATCAGACGTAAGTGATTTTAGAACCGAATTTGGATACAAAGTAGGTATTAACT
ATTCCCTTAATGCGAAGAAAGTTAATGTAGTTGATGAAGTTAAGAGATTAACCGATGGTAGAGGCGCTGATGTTACTATA
ATAGCCTCTGGATCTCCTTCTGCAATAGTCTCTGGTCTTAATGCTACTAGAAAGGGTGGTAGAGTTTTACTATTTGGTGT
GCCATATAAGGGGACGATACTTAATTACGATATTAGTGACCTCTTAAATAATGAGATCTCAATAATTCCTAGTAATGCTG
CTGTGGAAGAGGATACAAAGGAAGCTTTAGCTATAATAGCTAATAAGAAGATTGATGTAACTAAACTGATAACTCATAGA
TTCTCATTAGATGAGTTCAATGAGGCCGTAAGGATAGGTAAGGAGGGAAATGCCATAAAGGTAATAATATATAGTTGA

Upstream 100 bases:

>100_bases
ACTATGAATTCAAGTAAAACAACCAGTGTGAGTAAACCGAAAGTAATAATTAAATCCACTATTAATATAACGCAAGTATT
ACCATATATTCCTTTTTAAG

Downstream 100 bases:

>100_bases
AATAACTAGAAAGTACAAGATTAGACTTAATTTAAATAACTTAAGTGCTTTTTCAATATCATCCTCACATGGTAAATTTC
CAAAGCCCACTTTATAATGG

Product: sorbitol dehydrogenase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 345; Mature: 345

Protein sequence:

>345_residues
MVEGFSPMKSVIMENGKAVIKELPLPKLQQGDVLVKMKACGLCGTDIEKICGQYTASQPILGHEPTGIIQESTVDWLKPG
DRVFAHHHVPCYECYYCKKGSPTMCPYYRKTNLDPGGFSEYFRVPAWNVARGGVLKLPDNVSFEEGAFIEPLATVIRAQK
RVAIDNGDTVFIVGIGPMGLLHAMMAKVNKAGVIIASDVSDFRTEFGYKVGINYSLNAKKVNVVDEVKRLTDGRGADVTI
IASGSPSAIVSGLNATRKGGRVLLFGVPYKGTILNYDISDLLNNEISIIPSNAAVEEDTKEALAIIANKKIDVTKLITHR
FSLDEFNEAVRIGKEGNAIKVIIYS

Sequences:

>Translated_345_residues
MVEGFSPMKSVIMENGKAVIKELPLPKLQQGDVLVKMKACGLCGTDIEKICGQYTASQPILGHEPTGIIQESTVDWLKPG
DRVFAHHHVPCYECYYCKKGSPTMCPYYRKTNLDPGGFSEYFRVPAWNVARGGVLKLPDNVSFEEGAFIEPLATVIRAQK
RVAIDNGDTVFIVGIGPMGLLHAMMAKVNKAGVIIASDVSDFRTEFGYKVGINYSLNAKKVNVVDEVKRLTDGRGADVTI
IASGSPSAIVSGLNATRKGGRVLLFGVPYKGTILNYDISDLLNNEISIIPSNAAVEEDTKEALAIIANKKIDVTKLITHR
FSLDEFNEAVRIGKEGNAIKVIIYS
>Mature_345_residues
MVEGFSPMKSVIMENGKAVIKELPLPKLQQGDVLVKMKACGLCGTDIEKICGQYTASQPILGHEPTGIIQESTVDWLKPG
DRVFAHHHVPCYECYYCKKGSPTMCPYYRKTNLDPGGFSEYFRVPAWNVARGGVLKLPDNVSFEEGAFIEPLATVIRAQK
RVAIDNGDTVFIVGIGPMGLLHAMMAKVNKAGVIIASDVSDFRTEFGYKVGINYSLNAKKVNVVDEVKRLTDGRGADVTI
IASGSPSAIVSGLNATRKGGRVLLFGVPYKGTILNYDISDLLNNEISIIPSNAAVEEDTKEALAIIANKKIDVTKLITHR
FSLDEFNEAVRIGKEGNAIKVIIYS

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=357, Percent_Identity=30.5322128851541, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI156523966, Length=355, Percent_Identity=24.5070422535211, Blast_Score=75, Evalue=1e-13,
Organism=Homo sapiens, GI34577061, Length=342, Percent_Identity=23.9766081871345, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI4501933, Length=357, Percent_Identity=23.8095238095238, Blast_Score=71, Evalue=2e-12,
Organism=Homo sapiens, GI4501939, Length=349, Percent_Identity=24.3553008595989, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI71565154, Length=384, Percent_Identity=25.2604166666667, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1790045, Length=334, Percent_Identity=29.6407185628743, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI87082125, Length=347, Percent_Identity=29.971181556196, Blast_Score=128, Evalue=4e-31,
Organism=Escherichia coli, GI226510992, Length=333, Percent_Identity=30.3303303303303, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1788407, Length=336, Percent_Identity=28.2738095238095, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI1788073, Length=340, Percent_Identity=27.6470588235294, Blast_Score=115, Evalue=6e-27,
Organism=Escherichia coli, GI1788075, Length=339, Percent_Identity=27.1386430678466, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1790718, Length=321, Percent_Identity=27.7258566978193, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787863, Length=339, Percent_Identity=27.7286135693215, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1786825, Length=379, Percent_Identity=25.3298153034301, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI87081918, Length=294, Percent_Identity=29.2517006802721, Blast_Score=88, Evalue=8e-19,
Organism=Escherichia coli, GI1786552, Length=354, Percent_Identity=24.5762711864407, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17562876, Length=345, Percent_Identity=31.304347826087, Blast_Score=148, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI17562878, Length=353, Percent_Identity=28.0453257790368, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71988145, Length=315, Percent_Identity=23.4920634920635, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6319955, Length=354, Percent_Identity=29.6610169491525, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6322619, Length=354, Percent_Identity=29.3785310734463, Blast_Score=133, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6323099, Length=359, Percent_Identity=28.133704735376, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6323961, Length=333, Percent_Identity=27.027027027027, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319621, Length=309, Percent_Identity=27.1844660194175, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6319258, Length=342, Percent_Identity=23.9766081871345, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324486, Length=333, Percent_Identity=25.8258258258258, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319257, Length=356, Percent_Identity=24.7191011235955, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323729, Length=306, Percent_Identity=25.8169934640523, Blast_Score=66, Evalue=8e-12,
Organism=Drosophila melanogaster, GI17137530, Length=339, Percent_Identity=30.0884955752212, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI17737897, Length=341, Percent_Identity=29.0322580645161, Blast_Score=136, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 37556; Mature: 37556

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEGFSPMKSVIMENGKAVIKELPLPKLQQGDVLVKMKACGLCGTDIEKICGQYTASQPI
CCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCC
LGHEPTGIIQESTVDWLKPGDRVFAHHHVPCYECYYCKKGSPTMCPYYRKTNLDPGGFSE
CCCCCCCCEECCCHHHHCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHH
YFRVPAWNVARGGVLKLPDNVSFEEGAFIEPLATVIRAQKRVAIDNGDTVFIVGIGPMGL
HHCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHEEEECCCCEEEEEEECHHHH
LHAMMAKVNKAGVIIASDVSDFRTEFGYKVGINYSLNAKKVNVVDEVKRLTDGRGADVTI
HHHHHHHHCCCCEEEECCHHHHHHHCCEEEEEEEECCCCEECHHHHHHHHHCCCCCCEEE
IASGSPSAIVSGLNATRKGGRVLLFGVPYKGTILNYDISDLLNNEISIIPSNAAVEEDTK
EECCCCHHHHHCCCCCCCCCEEEEEECCCCCEEEECCHHHHCCCCEEEEECCCCCCHHHH
EALAIIANKKIDVTKLITHRFSLDEFNEAVRIGKEGNAIKVIIYS
HHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEEC
>Mature Secondary Structure
MVEGFSPMKSVIMENGKAVIKELPLPKLQQGDVLVKMKACGLCGTDIEKICGQYTASQPI
CCCCHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHCCCCCCCCC
LGHEPTGIIQESTVDWLKPGDRVFAHHHVPCYECYYCKKGSPTMCPYYRKTNLDPGGFSE
CCCCCCCCEECCCHHHHCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHH
YFRVPAWNVARGGVLKLPDNVSFEEGAFIEPLATVIRAQKRVAIDNGDTVFIVGIGPMGL
HHCCCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHEEEECCCCEEEEEEECHHHH
LHAMMAKVNKAGVIIASDVSDFRTEFGYKVGINYSLNAKKVNVVDEVKRLTDGRGADVTI
HHHHHHHHCCCCEEEECCHHHHHHHCCEEEEEEEECCCCEECHHHHHHHHHCCCCCCEEE
IASGSPSAIVSGLNATRKGGRVLLFGVPYKGTILNYDISDLLNNEISIIPSNAAVEEDTK
EECCCCHHHHHCCCCCCCCCEEEEEECCCCCEEEECCHHHHCCCCEEEEECCCCCCHHHH
EALAIIANKKIDVTKLITHRFSLDEFNEAVRIGKEGNAIKVIIYS
HHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1460002; 9384377; 8195086 [H]