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The map label for this gene is cobQ [H]

Identifier: 15899932

GI number: 15899932

Start: 2977559

End: 2978956

Strand: Reverse

Name: cobQ [H]

Synonym: SSO3233

Alternate gene names: 15899932

Gene position: 2978956-2977559 (Counterclockwise)

Preceding gene: 15899935

Following gene: 15899931

Centisome position: 99.56

GC content: 36.48

Gene sequence:

>1398_bases
GTGTCAATAATAGTAGCTTCGACAATGAGCGATTCTGGTAAATCATTTATAACCACTGGCTTAGTGAGGATTCTAAACGC
AAAACCACTTAAAATTCAGAACATGTCTTTGAATAGTATTTCGACTTGGGATGGAGGAGAAATAGCTTTTATACAAGCAT
ACCAGGCTTGGGGAGCTGGATTAAGACCAGAGAGGTATATGAACCCAATCTTGCTTAAGATAATGGGAAAGGGTCTTGAA
GTAATCTTTATGGGGAGTTCGCTTGGTATCATGAGTGGCAATGAGTATTACAATTTGGCAACTGAGAAGTTATGGAAAAA
GGTCTCACAATTCTTATCAAACGATGTTGTGATAGAGGCGGCAGGGGGTATAGGAGAACCGAATTTTATCGACAAGGATA
TTACAGCAATAAAAGTAATGAAGGAAAAGAGAATTCCGGCTATTTTAGTATTAGATATAGATAGGGGAGGCGCATTTGCA
TCCGCCTATGGTACTTATATGATGCTTCCTCAATCCGTTAGAGAGAACCTGAAAGGGTTCATTATTAATAAATTTAGAGG
AGATGAAAAGTTTCTGAATGACGCCATAAAATGGCTTGAAGAGAAAACTGGAATGAGATATTTAGGTTATCTGCCCTTTT
TTAGTGAGCCTCCCATAATGCCGGAAGATTCTATGAATGTTTACAATTTTGGCGATGGCAATGTTGAAGTAGCCATAATT
GCCTATCCTTACATGAGTAATTTCAATGAGTTTTACGCCTTAGTCAAGTCTAATGCACGTGTATCCTTCGTAAGAAAGCC
CAAAGAAACTGCAAAGGCAGATCTTGTAATTTTACCGGGTAGTAGAAATACTTTAGAATCGCTGAAGTGGTTGGTGGAAA
ACGGATTTGTAGAACAGCTAAGGCAAAGATCTATGAACATTTTAGGTATTTGCGGTGGTTTCCAAATTTTAGGTAAAAAG
ATTGTGGATCCTTACGGGATTGAGGCGGGATATCCTACAGAATATGAAGGGTTAGGAATATTTGATATTAATGTACATTA
TAGCAAGGATAAGGTTGTTTCAATGACAACTGGCATTTCTGAATATGGAGAAATGAATGGTTATGAAATTAGAAGAGGAG
TAATAAAATACATGGGTGAAAAGCCTTTGCTTAAGATAACATTAAGGAATGGTGAAAAAGTAAGCGTTGAGGATGGGGCA
AAAAAGGATAATATCATTGGTATAAGTGTACATGGAACCTTATTTTCTGATGGAATGAAAAAATTACTGAAGGAGTTCAA
TATTAATATTCACTCCCAATCGATGGAAGAAGAAATCAAACAGCAAGCAATTCGTGTAGAGCAATTTTTAAGGAAATATT
TACATATTGACGAAATAGTCGAAATTTATAAGCAATGA

Upstream 100 bases:

>100_bases
GCACTACTCTTAACTGGTATTGCTGTGAAAAACGAAAACTGCGCAAGTATCTCTTTTAGGTGCATGTGAATTTATTTAGT
AGCCGAGTTGATATGATTAA

Downstream 100 bases:

>100_bases
ATTTTTAGTCTTAACTAAATGTTTGATCCTGCTTCATTTGTCGAGGAAATAGGGCCGCAGTTGAGACAGAAGCTTGGCAA
TGAGAAAGTTTTAGCTGCGG

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 464

Protein sequence:

>465_residues
MSIIVASTMSDSGKSFITTGLVRILNAKPLKIQNMSLNSISTWDGGEIAFIQAYQAWGAGLRPERYMNPILLKIMGKGLE
VIFMGSSLGIMSGNEYYNLATEKLWKKVSQFLSNDVVIEAAGGIGEPNFIDKDITAIKVMKEKRIPAILVLDIDRGGAFA
SAYGTYMMLPQSVRENLKGFIINKFRGDEKFLNDAIKWLEEKTGMRYLGYLPFFSEPPIMPEDSMNVYNFGDGNVEVAII
AYPYMSNFNEFYALVKSNARVSFVRKPKETAKADLVILPGSRNTLESLKWLVENGFVEQLRQRSMNILGICGGFQILGKK
IVDPYGIEAGYPTEYEGLGIFDINVHYSKDKVVSMTTGISEYGEMNGYEIRRGVIKYMGEKPLLKITLRNGEKVSVEDGA
KKDNIIGISVHGTLFSDGMKKLLKEFNINIHSQSMEEEIKQQAIRVEQFLRKYLHIDEIVEIYKQ

Sequences:

>Translated_465_residues
MSIIVASTMSDSGKSFITTGLVRILNAKPLKIQNMSLNSISTWDGGEIAFIQAYQAWGAGLRPERYMNPILLKIMGKGLE
VIFMGSSLGIMSGNEYYNLATEKLWKKVSQFLSNDVVIEAAGGIGEPNFIDKDITAIKVMKEKRIPAILVLDIDRGGAFA
SAYGTYMMLPQSVRENLKGFIINKFRGDEKFLNDAIKWLEEKTGMRYLGYLPFFSEPPIMPEDSMNVYNFGDGNVEVAII
AYPYMSNFNEFYALVKSNARVSFVRKPKETAKADLVILPGSRNTLESLKWLVENGFVEQLRQRSMNILGICGGFQILGKK
IVDPYGIEAGYPTEYEGLGIFDINVHYSKDKVVSMTTGISEYGEMNGYEIRRGVIKYMGEKPLLKITLRNGEKVSVEDGA
KKDNIIGISVHGTLFSDGMKKLLKEFNINIHSQSMEEEIKQQAIRVEQFLRKYLHIDEIVEIYKQ
>Mature_464_residues
SIIVASTMSDSGKSFITTGLVRILNAKPLKIQNMSLNSISTWDGGEIAFIQAYQAWGAGLRPERYMNPILLKIMGKGLEV
IFMGSSLGIMSGNEYYNLATEKLWKKVSQFLSNDVVIEAAGGIGEPNFIDKDITAIKVMKEKRIPAILVLDIDRGGAFAS
AYGTYMMLPQSVRENLKGFIINKFRGDEKFLNDAIKWLEEKTGMRYLGYLPFFSEPPIMPEDSMNVYNFGDGNVEVAIIA
YPYMSNFNEFYALVKSNARVSFVRKPKETAKADLVILPGSRNTLESLKWLVENGFVEQLRQRSMNILGICGGFQILGKKI
VDPYGIEAGYPTEYEGLGIFDINVHYSKDKVVSMTTGISEYGEMNGYEIRRGVIKYMGEKPLLKITLRNGEKVSVEDGAK
KDNIIGISVHGTLFSDGMKKLLKEFNINIHSQSMEEEIKQQAIRVEQFLRKYLHIDEIVEIYKQ

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.10

Molecular weight: Translated: 52328; Mature: 52197

Theoretical pI: Translated: 8.25; Mature: 8.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIIVASTMSDSGKSFITTGLVRILNAKPLKIQNMSLNSISTWDGGEIAFIQAYQAWGAG
CEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEEEEEHHHCCC
LRPERYMNPILLKIMGKGLEVIFMGSSLGIMSGNEYYNLATEKLWKKVSQFLSNDVVIEA
CCCHHHCCHHHHHHHCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCEEEEE
AGGIGEPNFIDKDITAIKVMKEKRIPAILVLDIDRGGAFASAYGTYMMLPQSVRENLKGF
CCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHCCHHEECCHHHHCCCCEE
IINKFRGDEKFLNDAIKWLEEKTGMRYLGYLPFFSEPPIMPEDSMNVYNFGDGNVEVAII
EEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEEECCCCEEEEEE
AYPYMSNFNEFYALVKSNARVSFVRKPKETAKADLVILPGSRNTLESLKWLVENGFVEQL
EECCHHCHHHHHHHHHCCCEEEEEECCCHHCCCCEEEECCCCCHHHHHHHHHHCCHHHHH
RQRSMNILGICGGFQILGKKIVDPYGIEAGYPTEYEGLGIFDINVHYSKDKVVSMTTGIS
HHCCCCEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEEECCCCEEEEECCHH
EYGEMNGYEIRRGVIKYMGEKPLLKITLRNGEKVSVEDGAKKDNIIGISVHGTLFSDGMK
HHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCCCEEEEEEECHHHHHHHH
KLLKEFNINIHSQSMEEEIKQQAIRVEQFLRKYLHIDEIVEIYKQ
HHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCC
>Mature Secondary Structure 
SIIVASTMSDSGKSFITTGLVRILNAKPLKIQNMSLNSISTWDGGEIAFIQAYQAWGAG
EEEEEECCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCEEEEEEEHHHCCC
LRPERYMNPILLKIMGKGLEVIFMGSSLGIMSGNEYYNLATEKLWKKVSQFLSNDVVIEA
CCCHHHCCHHHHHHHCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHCCCEEEEE
AGGIGEPNFIDKDITAIKVMKEKRIPAILVLDIDRGGAFASAYGTYMMLPQSVRENLKGF
CCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHCCHHEECCHHHHCCCCEE
IINKFRGDEKFLNDAIKWLEEKTGMRYLGYLPFFSEPPIMPEDSMNVYNFGDGNVEVAII
EEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEEECCCCEEEEEE
AYPYMSNFNEFYALVKSNARVSFVRKPKETAKADLVILPGSRNTLESLKWLVENGFVEQL
EECCHHCHHHHHHHHHCCCEEEEEECCCHHCCCCEEEECCCCCHHHHHHHHHHCCHHHHH
RQRSMNILGICGGFQILGKKIVDPYGIEAGYPTEYEGLGIFDINVHYSKDKVVSMTTGIS
HHCCCCEEEEECCHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEEEEECCCCEEEEECCHH
EYGEMNGYEIRRGVIKYMGEKPLLKITLRNGEKVSVEDGAKKDNIIGISVHGTLFSDGMK
HHHCCCCHHHHHHHHHHCCCCCEEEEEECCCCEEEECCCCCCCCEEEEEEECHHHHHHHH
KLLKEFNINIHSQSMEEEIKQQAIRVEQFLRKYLHIDEIVEIYKQ
HHHHHHCCCEECHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]