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The map label for this gene is cobS

Identifier: 15899933

GI number: 15899933

Start: 2978992

End: 2979753

Strand: Direct

Name: cobS

Synonym: SSO3234

Alternate gene names: 15899933

Gene position: 2978992-2979753 (Clockwise)

Preceding gene: 15899930

Following gene: 15899934

Centisome position: 99.56

GC content: 33.73

Gene sequence:

>762_bases
ATGCACCTAAAAGAGATACTTGCGCAGTTTTCGTTTTTCACAGCAATACCAGTTAAGAGTAGTGCTTCCTTAGAGGAAAT
TGCGGAATCCTCTTACATATCGCCAATAATTGTGGGTATATCGTTGGGGTTAATTGAGAGTGTTGCGTACTTAATTCTAT
ATAGAATTCTTGGAGAACTTACTGGAATAGTATTGTTAGGTATAATAGAACTGCTTAGGGGATTTAACCATCTAGATGGC
TTACTGGACTTGGGGGATGCATTAATGATAAGGGGAAATAGAGAGAAAAAAATAAAAGCGTTGAAGGATGTTGAAGTTGG
CTCAGGAGGTATAGGTTTACTTTTAGTTTACTTATCAATACAAATAGTAGCTTTATTAAAATTAGATTTCTCGCTATACA
CCATATTCTATTTAATCTCATCTAATGTACTTTCCATGTCGTTAAGTCTTTATATACTTTCAACCATAAGTCCAATTCCA
GAAAGCAATTTAGGAAGAATCTTTCACGATAAATTGAAAGGAAAATCAACTATATTACTTCTAGAATTAATTCCTTTTAT
CTCATTATACAATGTGATAGTCTTTATAATATTTTACATGATAATGTATAAAATCTGTGGATCACTTGGAGGCTCCTCAG
GTGATATAGCTGGTGCGTCAATAACTCTTTCCTTTCCACTCTTCCTTATCACCGACGAGATAACCAATTTAAATTACTCA
CTACTATCTATCTTGTGTTATTTATTCTCGCACTTGCACTGA

Upstream 100 bases:

>100_bases
ACTAAGCCAGTGGTTATAAATGATTTACCAGAATCGCTCATTGTCGAAGCTACTATTATTGACACTTAATCATATCAACT
CGGCTACTAAATAAATTCAC

Downstream 100 bases:

>100_bases
TATGGGATTTAACGATTTCAGAACCGCCTATTTACATACACCCTGTAGTTTGGACTGGGAAAATATCGGAAATATTAATT
AAACCATATAAGGGCTATTT

Product: cobalamin synthase related protein

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MHLKEILAQFSFFTAIPVKSSASLEEIAESSYISPIIVGISLGLIESVAYLILYRILGELTGIVLLGIIELLRGFNHLDG
LLDLGDALMIRGNREKKIKALKDVEVGSGGIGLLLVYLSIQIVALLKLDFSLYTIFYLISSNVLSMSLSLYILSTISPIP
ESNLGRIFHDKLKGKSTILLLELIPFISLYNVIVFIIFYMIMYKICGSLGGSSGDIAGASITLSFPLFLITDEITNLNYS
LLSILCYLFSHLH

Sequences:

>Translated_253_residues
MHLKEILAQFSFFTAIPVKSSASLEEIAESSYISPIIVGISLGLIESVAYLILYRILGELTGIVLLGIIELLRGFNHLDG
LLDLGDALMIRGNREKKIKALKDVEVGSGGIGLLLVYLSIQIVALLKLDFSLYTIFYLISSNVLSMSLSLYILSTISPIP
ESNLGRIFHDKLKGKSTILLLELIPFISLYNVIVFIIFYMIMYKICGSLGGSSGDIAGASITLSFPLFLITDEITNLNYS
LLSILCYLFSHLH
>Mature_253_residues
MHLKEILAQFSFFTAIPVKSSASLEEIAESSYISPIIVGISLGLIESVAYLILYRILGELTGIVLLGIIELLRGFNHLDG
LLDLGDALMIRGNREKKIKALKDVEVGSGGIGLLLVYLSIQIVALLKLDFSLYTIFYLISSNVLSMSLSLYILSTISPIP
ESNLGRIFHDKLKGKSTILLLELIPFISLYNVIVFIIFYMIMYKICGSLGGSSGDIAGASITLSFPLFLITDEITNLNYS
LLSILCYLFSHLH

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin)

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBS_SULSO (Q97TZ6)

Other databases:

- EMBL:   AE006641
- PIR:   A90509
- RefSeq:   NP_344538.1
- ProteinModelPortal:   Q97TZ6
- GeneID:   1453247
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO3234
- NMPDR:   fig|273057.1.peg.2961
- HOGENOM:   HBG553455
- OMA:   CSILAVF
- ProtClustDB:   CLSK803649
- BioCyc:   SSOL273057:SSO3234-MONOMER
- HAMAP:   MF_00719
- InterPro:   IPR003805
- TIGRFAMs:   TIGR00317

Pfam domain/function: PF02654 CobS

EC number: 2.7.8.26

Molecular weight: Translated: 27832; Mature: 27832

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x10e63d20)-; HASH(0x11a6c08c)-; HASH(0x113c8e9c)-; HASH(0x1192f85c)-;

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLKEILAQFSFFTAIPVKSSASLEEIAESSYISPIIVGISLGLIESVAYLILYRILGEL
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TGIVLLGIIELLRGFNHLDGLLDLGDALMIRGNREKKIKALKDVEVGSGGIGLLLVYLSI
HHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHH
QIVALLKLDFSLYTIFYLISSNVLSMSLSLYILSTISPIPESNLGRIFHDKLKGKSTILL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHH
LELIPFISLYNVIVFIIFYMIMYKICGSLGGSSGDIAGASITLSFPLFLITDEITNLNYS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHEEHHHHCCCHH
LLSILCYLFSHLH
HHHHHHHHHHHCH
>Mature Secondary Structure
MHLKEILAQFSFFTAIPVKSSASLEEIAESSYISPIIVGISLGLIESVAYLILYRILGEL
CCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
TGIVLLGIIELLRGFNHLDGLLDLGDALMIRGNREKKIKALKDVEVGSGGIGLLLVYLSI
HHHHHHHHHHHHHHHHHHHHHHHCCCHHEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHH
QIVALLKLDFSLYTIFYLISSNVLSMSLSLYILSTISPIPESNLGRIFHDKLKGKSTILL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCHHHHH
LELIPFISLYNVIVFIIFYMIMYKICGSLGGSSGDIAGASITLSFPLFLITDEITNLNYS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECHHHHEEHHHHCCCHH
LLSILCYLFSHLH
HHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11427726