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The map label for this gene is ilvB-6 [H]

Identifier: 15899911

GI number: 15899911

Start: 2955990

End: 2957621

Strand: Reverse

Name: ilvB-6 [H]

Synonym: SSO3210

Alternate gene names: 15899911

Gene position: 2957621-2955990 (Counterclockwise)

Preceding gene: 15899913

Following gene: 15899910

Centisome position: 98.84

GC content: 38.05

Gene sequence:

>1632_bases
ATGATAAAATATTTAGTTATGAATGCCGGAAGGCTATTTTTATCTCTTCTTAAAGAAAGCGGGGTAAATAAAATATTTAT
AGTATCTGGTACGGATTACGCTTCATTAATAGAGGCTAAGGTCGAAGATTCCAGTTTACCAGAATTTGAAATAGTACCTC
ATGAGATTACTGCTATATCAACTGCAATAGGTTACGCACTAGGCAATAAGCTAAGTGCTGTTGCAGTTCACACTACACCT
GGAACGGCAAACGCATTGGGAGGTATTATGAGTGCGTTCACTTCCAGAATACCACTTTTAGTTATTGCGGGGAGGAGTCC
ATATACTGAGAAAGGCAATACTGCAAGCAGGAACTTGAGAATCCACTGGACACAAGAGGCTAGGGATCAAGGAGAATTGG
TTAGGCAATATGTCAAGTACGATTTTGAAATTAGGATGGCTGATCAGTTACCAGCAGTAGTATCTAGAGCAATTCAAATA
ATGATGAGTGAACCAAGAGGTCCAGTATATATTGTTTTGCCAAGAGAAGTTAGCATTCAAGAAGTTAATGAAGCTAGGAG
AATACCAATGGATTATTATGAACCCGCACCTTCTCCGGATAAAATTAACAAGGCTAAGGAAATGCTGGAAAAGTCAGAAA
GACCACTAATTATAACATGGAGGGCTGGAAGGAGAAAAGAATGGTTTGAATCCCTAAGGAGATTTGCAGATAATTATAAT
ATTCCAGTTCTGAACTACGCTGGCGAGGTTTTGAATTATCCCAGCAGTGGACCAATGGCTTTGGATAGGTTTGATTTACG
AAATAGTGATTTGTTATTAGTAGTGGAGGCAGAAGTGCCGTATTTTCCGAAGAAAATTGACCTAGATATTCCAATAGTTA
AGATTGATGTTGATCCATCTTATTCTTACATTCCATATTATGGCTTTAGATGTGATCTATGTATACAATCCACACCCAGC
AACTTCTTTGATTATATTTCGATTAGACCGAAGAGTTATGACGAGATTAAGGAGCTAAGGGCTAAGCAAGAAGAATATAA
GAAGCAAGAGATTGAGAGATTAAAGGATAAGAAACCAATTCATCCGAAATATTTATCATATGAAATTGGAATTGTTGCGT
CTGAGTACAATTTGGTAATATTTAATGAGTATCAATTTAATCCTAGATACGCTAGGCTAAATGAGTTTGGTTCTTACTTT
GCTGACTTGTCCGTAGGATATCTAGGCTTCGCATTAGGTGCTGGCGTTGGATATAAGATAGCTACTAACAAGGACGTACT
TATCACTACTGGTGATGGCTCATTTATATTTGGCGTTCCGGAAGCCTTCTATTATGTAGCATCGAAATATCCGACGATGG
TTGTGATTTATGATAATGGTGGCTGGTTGGCATCAGCTGAGGCAGTAGATGAGGTTTTTCCTGAGGGTTTAGCAAAAAGT
AAAAAGTATTATCCTGGCGCAGATTTTGATAGAAGGTTCGAGATTGGGAAGACTGTTGAAACTTTTCATGGCTACTATGA
ACTAGTTGAGGATCCTTGGGAGATTAAGCCTGCCTTAATAAGGGGCTTAGAGAAAATGAGAAGAGAGAATAAAATAGCTG
TGATTCAAGTTATAGTAGACAAGGTGAGATAA

Upstream 100 bases:

>100_bases
AGTTTCTTAAAGTAACAATACTCACTATTCGTTTTTCGTAATACAATAAATTATCGATCTTTAATATTTTCTTCGGGAGG
AAAGGTAGTATTTTATATTC

Downstream 100 bases:

>100_bases
TCATGAGGTTATGTAATAAGGACGTCTCACAAATTGGTTTTGGGACTTGGAAAATTGGAGGAGGGTATTGGAATCCAGAT
TATTCTAAGGATTCCCATTA

Product: acetolactate synthase catalytic subunit

Products: NA

Alternate protein names: ALS; Acetohydroxy-acid synthase [H]

Number of amino acids: Translated: 543; Mature: 543

Protein sequence:

>543_residues
MIKYLVMNAGRLFLSLLKESGVNKIFIVSGTDYASLIEAKVEDSSLPEFEIVPHEITAISTAIGYALGNKLSAVAVHTTP
GTANALGGIMSAFTSRIPLLVIAGRSPYTEKGNTASRNLRIHWTQEARDQGELVRQYVKYDFEIRMADQLPAVVSRAIQI
MMSEPRGPVYIVLPREVSIQEVNEARRIPMDYYEPAPSPDKINKAKEMLEKSERPLIITWRAGRRKEWFESLRRFADNYN
IPVLNYAGEVLNYPSSGPMALDRFDLRNSDLLLVVEAEVPYFPKKIDLDIPIVKIDVDPSYSYIPYYGFRCDLCIQSTPS
NFFDYISIRPKSYDEIKELRAKQEEYKKQEIERLKDKKPIHPKYLSYEIGIVASEYNLVIFNEYQFNPRYARLNEFGSYF
ADLSVGYLGFALGAGVGYKIATNKDVLITTGDGSFIFGVPEAFYYVASKYPTMVVIYDNGGWLASAEAVDEVFPEGLAKS
KKYYPGADFDRRFEIGKTVETFHGYYELVEDPWEIKPALIRGLEKMRRENKIAVIQVIVDKVR

Sequences:

>Translated_543_residues
MIKYLVMNAGRLFLSLLKESGVNKIFIVSGTDYASLIEAKVEDSSLPEFEIVPHEITAISTAIGYALGNKLSAVAVHTTP
GTANALGGIMSAFTSRIPLLVIAGRSPYTEKGNTASRNLRIHWTQEARDQGELVRQYVKYDFEIRMADQLPAVVSRAIQI
MMSEPRGPVYIVLPREVSIQEVNEARRIPMDYYEPAPSPDKINKAKEMLEKSERPLIITWRAGRRKEWFESLRRFADNYN
IPVLNYAGEVLNYPSSGPMALDRFDLRNSDLLLVVEAEVPYFPKKIDLDIPIVKIDVDPSYSYIPYYGFRCDLCIQSTPS
NFFDYISIRPKSYDEIKELRAKQEEYKKQEIERLKDKKPIHPKYLSYEIGIVASEYNLVIFNEYQFNPRYARLNEFGSYF
ADLSVGYLGFALGAGVGYKIATNKDVLITTGDGSFIFGVPEAFYYVASKYPTMVVIYDNGGWLASAEAVDEVFPEGLAKS
KKYYPGADFDRRFEIGKTVETFHGYYELVEDPWEIKPALIRGLEKMRRENKIAVIQVIVDKVR
>Mature_543_residues
MIKYLVMNAGRLFLSLLKESGVNKIFIVSGTDYASLIEAKVEDSSLPEFEIVPHEITAISTAIGYALGNKLSAVAVHTTP
GTANALGGIMSAFTSRIPLLVIAGRSPYTEKGNTASRNLRIHWTQEARDQGELVRQYVKYDFEIRMADQLPAVVSRAIQI
MMSEPRGPVYIVLPREVSIQEVNEARRIPMDYYEPAPSPDKINKAKEMLEKSERPLIITWRAGRRKEWFESLRRFADNYN
IPVLNYAGEVLNYPSSGPMALDRFDLRNSDLLLVVEAEVPYFPKKIDLDIPIVKIDVDPSYSYIPYYGFRCDLCIQSTPS
NFFDYISIRPKSYDEIKELRAKQEEYKKQEIERLKDKKPIHPKYLSYEIGIVASEYNLVIFNEYQFNPRYARLNEFGSYF
ADLSVGYLGFALGAGVGYKIATNKDVLITTGDGSFIFGVPEAFYYVASKYPTMVVIYDNGGWLASAEAVDEVFPEGLAKS
KKYYPGADFDRRFEIGKTVETFHGYYELVEDPWEIKPALIRGLEKMRRENKIAVIQVIVDKVR

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081685, Length=588, Percent_Identity=21.4285714285714, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788716, Length=496, Percent_Identity=23.3870967741935, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1787096, Length=476, Percent_Identity=21.4285714285714, Blast_Score=75, Evalue=9e-15,
Organism=Escherichia coli, GI1786717, Length=190, Percent_Identity=27.8947368421053, Blast_Score=68, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17531301, Length=501, Percent_Identity=22.7544910179641, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17531299, Length=501, Percent_Identity=22.7544910179641, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6323755, Length=516, Percent_Identity=20.1550387596899, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19922626, Length=443, Percent_Identity=26.410835214447, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61834; Mature: 61834

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKYLVMNAGRLFLSLLKESGVNKIFIVSGTDYASLIEAKVEDSSLPEFEIVPHEITAIS
CEEEEEECCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCCCCCEEECCHHHHHHH
TAIGYALGNKLSAVAVHTTPGTANALGGIMSAFTSRIPLLVIAGRSPYTEKGNTASRNLR
HHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEE
IHWTQEARDQGELVRQYVKYDFEIRMADQLPAVVSRAIQIMMSEPRGPVYIVLPREVSIQ
EEECHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHH
EVNEARRIPMDYYEPAPSPDKINKAKEMLEKSERPLIITWRAGRRKEWFESLRRFADNYN
HHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCC
IPVLNYAGEVLNYPSSGPMALDRFDLRNSDLLLVVEAEVPYFPKKIDLDIPIVKIDVDPS
CEEEECCHHHHCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCEECCCCEEEEEECCCC
YSYIPYYGFRCDLCIQSTPSNFFDYISIRPKSYDEIKELRAKQEEYKKQEIERLKDKKPI
CCCCCEECCEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
HPKYLSYEIGIVASEYNLVIFNEYQFNPRYARLNEFGSYFADLSVGYLGFALGAGVGYKI
CCCEEEEEEEEEEECCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
ATNKDVLITTGDGSFIFGVPEAFYYVASKYPTMVVIYDNGGWLASAEAVDEVFPEGLAKS
ECCCEEEEEECCCCEEEECHHHHHHHHCCCCEEEEEECCCCEEECHHHHHHHHHHHHHHC
KKYYPGADFDRRFEIGKTVETFHGYYELVEDPWEIKPALIRGLEKMRRENKIAVIQVIVD
CCCCCCCCCCCCEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEHHH
KVR
HCC
>Mature Secondary Structure
MIKYLVMNAGRLFLSLLKESGVNKIFIVSGTDYASLIEAKVEDSSLPEFEIVPHEITAIS
CEEEEEECCHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCCCCCCEEECCHHHHHHH
TAIGYALGNKLSAVAVHTTPGTANALGGIMSAFTSRIPLLVIAGRSPYTEKGNTASRNLR
HHHHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEE
IHWTQEARDQGELVRQYVKYDFEIRMADQLPAVVSRAIQIMMSEPRGPVYIVLPREVSIQ
EEECHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHH
EVNEARRIPMDYYEPAPSPDKINKAKEMLEKSERPLIITWRAGRRKEWFESLRRFADNYN
HHHHHHCCCCHHCCCCCCHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCCC
IPVLNYAGEVLNYPSSGPMALDRFDLRNSDLLLVVEAEVPYFPKKIDLDIPIVKIDVDPS
CEEEECCHHHHCCCCCCCCEEEEECCCCCCEEEEEECCCCCCCCEECCCCEEEEEECCCC
YSYIPYYGFRCDLCIQSTPSNFFDYISIRPKSYDEIKELRAKQEEYKKQEIERLKDKKPI
CCCCCEECCEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCC
HPKYLSYEIGIVASEYNLVIFNEYQFNPRYARLNEFGSYFADLSVGYLGFALGAGVGYKI
CCCEEEEEEEEEEECCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
ATNKDVLITTGDGSFIFGVPEAFYYVASKYPTMVVIYDNGGWLASAEAVDEVFPEGLAKS
ECCCEEEEEECCCCEEEECHHHHHHHHCCCCEEEEEECCCCEEECHHHHHHHHHHHHHHC
KKYYPGADFDRRFEIGKTVETFHGYYELVEDPWEIKPALIRGLEKMRRENKIAVIQVIVD
CCCCCCCCCCCCEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCEEEEEEHHH
KVR
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]