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The map label for this gene is lplA-2 [H]

Identifier: 15899865

GI number: 15899865

Start: 2910069

End: 2910863

Strand: Direct

Name: lplA-2 [H]

Synonym: SSO3159

Alternate gene names: 15899865

Gene position: 2910069-2910863 (Clockwise)

Preceding gene: 15899864

Following gene: 15899869

Centisome position: 97.25

GC content: 35.35

Gene sequence:

>795_bases
TTGAAAATGCTAAGAATCATAGTTGATGGTCCTAGAGATCCTTATTATAATATGGCAATTGATGAGGCAATAATGCTGAC
TAGAAATAGTGTTAATTATGATACGTTAAGACTATACATGTGGTCTCCATCAGGTGTAAGTTTAGGTAGGGGACAAAACG
CTAATGCTGCGTACTTAGATAACATTAAGGAGTTAGGGTTTAAATTAGTGAGAAGGCCAACTGGAGGTGGGGCATTACTA
CATCCAGAAGATAACGAAATTACATATAGTGTGGTATTGTCAGCTAGCAATACAATAGCTAAATTAAGTGTTGATGAGTC
TGCCGTAGAGATCGCTAAGGGTATATTATACACTCTTCAAATCTTAGGTGAAAACGTTGACATTAGAGGTTTGGGGGATA
TGAAAAAACACGACTTATGTTATTTGAGAAGTGGATCAAGCGATGTGATAGTTGCTGGGAGAAAAATATCTGGATCAGCC
CAAGTAAGGGATGATAAAGCGTTATTACAGCATGGTACATTGCTACTTAGATTTGAACCAGAAATTTGGTTAAAGGTGAT
TAGAGCACCAGGAGTTACTGAGGAGTTCTTAAAAAGTAGGATAGTAGGCTTATTTGAATTTATGGAAGTTGAAATAGATC
AAATTTTTGACGCAATGATAAAAGGTTTCAGTAAGGCGTTAAATGAAATAGACGTATTTATGGGAAGTCTGACTCCGAAG
GAGATAAAATTATCTAATAAACTTTACAAAGAAAAATATTCTAACGAGAAGTGGAATTTACTAGGTATCCAATAA

Upstream 100 bases:

>100_bases
GAACTTGAGGAAATAGCCTACTCAATGGGTTTCTCAGCTGTGATCTCCTTACCATTAGCTAGAACCTCTTATAAGGCGAA
AGAGGCCTATTTTAGGGCGA

Downstream 100 bases:

>100_bases
ATCAGCAATATGGTATACGTCATAACCCATTGACCTTATGGCTAATGCACAGACACTATTAGTTACAATAACCTTTTTAG
CTTCGCTTTTGGCTATCTTA

Product: lipoate-protein ligase A (lplA-2)

Products: lipoyl-AMP; pyrophosphate; N6-lipoyl-lysine [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MKMLRIIVDGPRDPYYNMAIDEAIMLTRNSVNYDTLRLYMWSPSGVSLGRGQNANAAYLDNIKELGFKLVRRPTGGGALL
HPEDNEITYSVVLSASNTIAKLSVDESAVEIAKGILYTLQILGENVDIRGLGDMKKHDLCYLRSGSSDVIVAGRKISGSA
QVRDDKALLQHGTLLLRFEPEIWLKVIRAPGVTEEFLKSRIVGLFEFMEVEIDQIFDAMIKGFSKALNEIDVFMGSLTPK
EIKLSNKLYKEKYSNEKWNLLGIQ

Sequences:

>Translated_264_residues
MKMLRIIVDGPRDPYYNMAIDEAIMLTRNSVNYDTLRLYMWSPSGVSLGRGQNANAAYLDNIKELGFKLVRRPTGGGALL
HPEDNEITYSVVLSASNTIAKLSVDESAVEIAKGILYTLQILGENVDIRGLGDMKKHDLCYLRSGSSDVIVAGRKISGSA
QVRDDKALLQHGTLLLRFEPEIWLKVIRAPGVTEEFLKSRIVGLFEFMEVEIDQIFDAMIKGFSKALNEIDVFMGSLTPK
EIKLSNKLYKEKYSNEKWNLLGIQ
>Mature_264_residues
MKMLRIIVDGPRDPYYNMAIDEAIMLTRNSVNYDTLRLYMWSPSGVSLGRGQNANAAYLDNIKELGFKLVRRPTGGGALL
HPEDNEITYSVVLSASNTIAKLSVDESAVEIAKGILYTLQILGENVDIRGLGDMKKHDLCYLRSGSSDVIVAGRKISGSA
QVRDDKALLQHGTLLLRFEPEIWLKVIRAPGVTEEFLKSRIVGLFEFMEVEIDQIFDAMIKGFSKALNEIDVFMGSLTPK
EIKLSNKLYKEKYSNEKWNLLGIQ

Specific function: Catalyzes Both The ATP-Dependent Activation Of Exogenously Supplied Lipoate To Lipoyl-Amp And The Transfer Of The Activated Lipoyl On The Lipoate-Dependent Enzymes. Creates An Amide Linkage That Joins The Free Carboxyl Group Of Lipoic Acid To The Epsilon-

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 29594; Mature: 29594

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMLRIIVDGPRDPYYNMAIDEAIMLTRNSVNYDTLRLYMWSPSGVSLGRGQNANAAYLD
CCEEEEEEECCCCCCCCHHHHHEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHH
NIKELGFKLVRRPTGGGALLHPEDNEITYSVVLSASNTIAKLSVDESAVEIAKGILYTLQ
HHHHHHHHHHCCCCCCCEEECCCCCCEEEEEEEECCCCEEEEECCHHHHHHHHHHHHHHH
ILGENVDIRGLGDMKKHDLCYLRSGSSDVIVAGRKISGSAQVRDDKALLQHGTLLLRFEP
HHCCCCCEEECCCCCCCCEEEEECCCCCEEEEEEEECCCCEECHHHHHHHCCEEEEEECH
EIWLKVIRAPGVTEEFLKSRIVGLFEFMEVEIDQIFDAMIKGFSKALNEIDVFMGSLTPK
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
EIKLSNKLYKEKYSNEKWNLLGIQ
HHHHHHHHHHHHHCCCCEEEEECC
>Mature Secondary Structure
MKMLRIIVDGPRDPYYNMAIDEAIMLTRNSVNYDTLRLYMWSPSGVSLGRGQNANAAYLD
CCEEEEEEECCCCCCCCHHHHHEEEEECCCCCEEEEEEEEECCCCCCCCCCCCCCHHHHH
NIKELGFKLVRRPTGGGALLHPEDNEITYSVVLSASNTIAKLSVDESAVEIAKGILYTLQ
HHHHHHHHHHCCCCCCCEEECCCCCCEEEEEEEECCCCEEEEECCHHHHHHHHHHHHHHH
ILGENVDIRGLGDMKKHDLCYLRSGSSDVIVAGRKISGSAQVRDDKALLQHGTLLLRFEP
HHCCCCCEEECCCCCCCCEEEEECCCCCEEEEEEEECCCCEECHHHHHHHCCEEEEEECH
EIWLKVIRAPGVTEEFLKSRIVGLFEFMEVEIDQIFDAMIKGFSKALNEIDVFMGSLTPK
HHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
EIKLSNKLYKEKYSNEKWNLLGIQ
HHHHHHHHHHHHHCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lipoic acid; ATP [C]

Specific reaction: Catalyzes first the reaction of lipoic acid and ATP to form lipoyl-AMP and pyrophosphate, then the formation N6-lipoyl-lysine from a specific lysine residue in lipoate-dependent enzymes [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Other Carbon-Nitrogen Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]