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The map label for this gene is lipA

Identifier: 15899864

GI number: 15899864

Start: 2909237

End: 2910094

Strand: Direct

Name: lipA

Synonym: SSO3158

Alternate gene names: 15899864

Gene position: 2909237-2910094 (Clockwise)

Preceding gene: 15899863

Following gene: 15899865

Centisome position: 97.23

GC content: 39.51

Gene sequence:

>858_bases
ATGGAGAAAAAAGTTCAAATTGCAGTTTACGAGAATGAAAACTTCAAAAGAGTAGCAGAGATTGTAAAGAAGAAAAGTAT
TGCTACTGTGTGTGAAGAGGCGCTATGCCCAAACATTATGGAATGCTGGGGATCTGGAACTGCAACATTTATGATCATGG
GGAGCATTTGTACTAGAGGTTGTAGGTTTTGTTATGTGTTAAAGGGAAAGCCCTCACCATTGGACGATGAAGAACCTAAA
AGAGTTGCAGAAGCGGTAAAGGAGATGGAACTGGACTACGTTGTAATAACCAGTGTTGATAGAGACGACCTATCCGATGG
GGGAGCACAACATTTCGCTAACGTGGTGAAAACAGTAAAAGAACTTAACCCGGGTATAATAGTTGAAGTGTTAACTCCAG
ACTTTAGGGGGAATATTGACGCAGTTAAAAAAGTAATTGATGCTGGAGTTGACGTATTTGCACATAACGTTGAGACCGTA
AGAAGACTAACGCCAATAGTAAGGGACCCTAGAGCCTCATATGAGCAAAGTCTCAATGTTTTAAAATATGCTGAAAACGT
GATAAAAAAATCCTCAATCCTCTTAGGTCTCGGTGAGACTTGGGATGAGATAGTAGAAACGATGAGAGATTTGAGAAGTG
TAGGAGTTAGTATATTGGTACTTTCACAATATATGAGACCTAGTCGTAAACAATTGGAGGTTAAGAAGCGTTATACTTTA
GAAGAGTTTAAGGAACTTGAGGAAATAGCCTACTCAATGGGTTTCTCAGCTGTGATCTCCTTACCATTAGCTAGAACCTC
TTATAAGGCGAAAGAGGCCTATTTTAGGGCGATTGAAAATGCTAAGAATCATAGTTGA

Upstream 100 bases:

>100_bases
GATTTTATCCCAGTACAATGGTAAGGTAATTAAAGTTTTGGTAAGAGAAGGAGATAAGGTATCGCCAGGATCAGTTATAG
CCTTAATAGAGGTTTAGATA

Downstream 100 bases:

>100_bases
TGGTCCTAGAGATCCTTATTATAATATGGCAATTGATGAGGCAATAATGCTGACTAGAAATAGTGTTAATTATGATACGT
TAAGACTATACATGTGGTCT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 285; Mature: 285

Protein sequence:

>285_residues
MEKKVQIAVYENENFKRVAEIVKKKSIATVCEEALCPNIMECWGSGTATFMIMGSICTRGCRFCYVLKGKPSPLDDEEPK
RVAEAVKEMELDYVVITSVDRDDLSDGGAQHFANVVKTVKELNPGIIVEVLTPDFRGNIDAVKKVIDAGVDVFAHNVETV
RRLTPIVRDPRASYEQSLNVLKYAENVIKKSSILLGLGETWDEIVETMRDLRSVGVSILVLSQYMRPSRKQLEVKKRYTL
EEFKELEEIAYSMGFSAVISLPLARTSYKAKEAYFRAIENAKNHS

Sequences:

>Translated_285_residues
MEKKVQIAVYENENFKRVAEIVKKKSIATVCEEALCPNIMECWGSGTATFMIMGSICTRGCRFCYVLKGKPSPLDDEEPK
RVAEAVKEMELDYVVITSVDRDDLSDGGAQHFANVVKTVKELNPGIIVEVLTPDFRGNIDAVKKVIDAGVDVFAHNVETV
RRLTPIVRDPRASYEQSLNVLKYAENVIKKSSILLGLGETWDEIVETMRDLRSVGVSILVLSQYMRPSRKQLEVKKRYTL
EEFKELEEIAYSMGFSAVISLPLARTSYKAKEAYFRAIENAKNHS
>Mature_285_residues
MEKKVQIAVYENENFKRVAEIVKKKSIATVCEEALCPNIMECWGSGTATFMIMGSICTRGCRFCYVLKGKPSPLDDEEPK
RVAEAVKEMELDYVVITSVDRDDLSDGGAQHFANVVKTVKELNPGIIVEVLTPDFRGNIDAVKKVIDAGVDVFAHNVETV
RRLTPIVRDPRASYEQSLNVLKYAENVIKKSSILLGLGETWDEIVETMRDLRSVGVSILVLSQYMRPSRKQLEVKKRYTL
EEFKELEEIAYSMGFSAVISLPLARTSYKAKEAYFRAIENAKNHS

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=277, Percent_Identity=50.5415162454874, Blast_Score=286, Evalue=2e-77,
Organism=Homo sapiens, GI37577164, Length=232, Percent_Identity=53.448275862069, Blast_Score=255, Evalue=4e-68,
Organism=Escherichia coli, GI1786846, Length=254, Percent_Identity=48.0314960629921, Blast_Score=246, Evalue=2e-66,
Organism=Caenorhabditis elegans, GI32564533, Length=292, Percent_Identity=47.6027397260274, Blast_Score=272, Evalue=2e-73,
Organism=Saccharomyces cerevisiae, GI6324770, Length=293, Percent_Identity=44.7098976109215, Blast_Score=264, Evalue=1e-71,
Organism=Drosophila melanogaster, GI221513272, Length=281, Percent_Identity=50.8896797153025, Blast_Score=285, Evalue=2e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_SULSO (Q97U63)

Other databases:

- EMBL:   AE006641
- PIR:   D90500
- RefSeq:   NP_344469.1
- ProteinModelPortal:   Q97U63
- GeneID:   1453178
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO3158
- NMPDR:   fig|273057.1.peg.2892
- HOGENOM:   HBG284542
- OMA:   CRFCYVK
- PhylomeDB:   Q97U63
- ProtClustDB:   PRK05481
- BioCyc:   SSOL273057:SSO3158-MONOMER
- BRENDA:   2.8.1.8
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 32111; Mature: 32111

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKKVQIAVYENENFKRVAEIVKKKSIATVCEEALCPNIMECWGSGTATFMIMGSICTRG
CCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHCC
CRFCYVLKGKPSPLDDEEPKRVAEAVKEMELDYVVITSVDRDDLSDGGAQHFANVVKTVK
CCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCHHCCCCCHHHHHHHHHHHHH
ELNPGIIVEVLTPDFRGNIDAVKKVIDAGVDVFAHNVETVRRLTPIVRDPRASYEQSLNV
HCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LKYAENVIKKSSILLGLGETWDEIVETMRDLRSVGVSILVLSQYMRPSRKQLEVKKRYTL
HHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCH
EEFKELEEIAYSMGFSAVISLPLARTSYKAKEAYFRAIENAKNHS
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEKKVQIAVYENENFKRVAEIVKKKSIATVCEEALCPNIMECWGSGTATFMIMGSICTRG
CCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHHHCC
CRFCYVLKGKPSPLDDEEPKRVAEAVKEMELDYVVITSVDRDDLSDGGAQHFANVVKTVK
CCEEEEECCCCCCCCCCCHHHHHHHHHHHCCCEEEEEECCHHCCCCCHHHHHHHHHHHHH
ELNPGIIVEVLTPDFRGNIDAVKKVIDAGVDVFAHNVETVRRLTPIVRDPRASYEQSLNV
HCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LKYAENVIKKSSILLGLGETWDEIVETMRDLRSVGVSILVLSQYMRPSRKQLEVKKRYTL
HHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCH
EEFKELEEIAYSMGFSAVISLPLARTSYKAKEAYFRAIENAKNHS
HHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726