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The map label for this gene is lplA-1 [H]

Identifier: 15899862

GI number: 15899862

Start: 2908209

End: 2908952

Strand: Reverse

Name: lplA-1 [H]

Synonym: SSO3157

Alternate gene names: 15899862

Gene position: 2908952-2908209 (Counterclockwise)

Preceding gene: 15899866

Following gene: 15899860

Centisome position: 97.22

GC content: 34.95

Gene sequence:

>744_bases
GTGAAGCTTAGGGTATTATTAACGGAATATCCCAATGACCCCTATTTGAATGTTGCAATGGACGAAGCGCTTTTATTACA
CAGTAAATACGTCCCTATTTTAAGAATATGGAGAAACGAGAAGAGTGTAGTTTTAGGAATATTGTCAAGTATAAGTGATG
AGGTTAATTTAGAAATGATTAAGAAATACGATGTGAAATTGGCTAGGAGGATTAGCGGTGGAGGGTCTGTCTTTCACGAT
ATGGGGAACATAAACTATACTTTCATAACAAGCGGTCAAGGTGGCATAGATTATCTTTACGGTTATTTGCTAAGAGGTAC
GATTAGCGCAATAGAGAATTTGGTAAATGATAGAGTTGAGGTTTATAACGAAACTGATATTGCATTTAAGGGTTACAAGA
TTTCTGGCAATGCTGGATATATAAATGAGGATAAGTATCTTTTACACGGCACTCTTCTCGTTTCATCTAATTTGGATATA
TTGCATAAGGTACTAATAATACCTCCTAAAAATTACAGAAAAAAAGATATTAACATGATAAAGTATCGAGTAAGCAATCT
CTCAAACTTGGTAAAAGGCGATATAACCTATGATGAAGCGATTGAGGCTTTCATAAGAGGTTTCTCGTCACTCTTACGTT
GCGATGACTATTTTTTCGACGATATAAGTGATGAGGAATTGAAGCTTGCAATGAGGCTGGCTAACGAAAAGTACTCAAAA
AAAGAGTTCATTTATAAACGTTAA

Upstream 100 bases:

>100_bases
ATTTCCCCTCCCCAATCCCTTCTTCTAGGCCATATATCTTCTGGGATCTTTAACTCGGCTTGCACAAACTATAAATTGAT
AATGGAAAATAAAAAGCATA

Downstream 100 bases:

>100_bases
CATCTCAACTTCTTTGCAACTTCTGCAACTCTTTTGCCTAAGAACTTAGCTATATTGATCTCATTTTCATCTAACTCCTT
CTTATTTCCCAAGTGCGATG

Product: lipoate-protein ligase A (lplA-1)

Products: NA

Alternate protein names: Lipoate--protein ligase subunit 1 [H]

Number of amino acids: Translated: 247; Mature: 247

Protein sequence:

>247_residues
MKLRVLLTEYPNDPYLNVAMDEALLLHSKYVPILRIWRNEKSVVLGILSSISDEVNLEMIKKYDVKLARRISGGGSVFHD
MGNINYTFITSGQGGIDYLYGYLLRGTISAIENLVNDRVEVYNETDIAFKGYKISGNAGYINEDKYLLHGTLLVSSNLDI
LHKVLIIPPKNYRKKDINMIKYRVSNLSNLVKGDITYDEAIEAFIRGFSSLLRCDDYFFDDISDEELKLAMRLANEKYSK
KEFIYKR

Sequences:

>Translated_247_residues
MKLRVLLTEYPNDPYLNVAMDEALLLHSKYVPILRIWRNEKSVVLGILSSISDEVNLEMIKKYDVKLARRISGGGSVFHD
MGNINYTFITSGQGGIDYLYGYLLRGTISAIENLVNDRVEVYNETDIAFKGYKISGNAGYINEDKYLLHGTLLVSSNLDI
LHKVLIIPPKNYRKKDINMIKYRVSNLSNLVKGDITYDEAIEAFIRGFSSLLRCDDYFFDDISDEELKLAMRLANEKYSK
KEFIYKR
>Mature_247_residues
MKLRVLLTEYPNDPYLNVAMDEALLLHSKYVPILRIWRNEKSVVLGILSSISDEVNLEMIKKYDVKLARRISGGGSVFHD
MGNINYTFITSGQGGIDYLYGYLLRGTISAIENLVNDRVEVYNETDIAFKGYKISGNAGYINEDKYLLHGTLLVSSNLDI
LHKVLIIPPKNYRKKDINMIKYRVSNLSNLVKGDITYDEAIEAFIRGFSSLLRCDDYFFDDISDEELKLAMRLANEKYSK
KEFIYKR

Specific function: Lipoate-protein ligase catalyzes both the ATP-dependent activation of exogenously supplied lipoate to lipoyl-AMP and the transfer of the activated lipoyl onto the lipoyl domains of lipoate-dependent enzymes. This subunit can alone only catalyze the lipoat

COG id: COG0095

COG function: function code H; Lipoate-protein ligase A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lplA family [H]

Homologues:

Organism=Homo sapiens, GI7706252, Length=186, Percent_Identity=33.8709677419355, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI21729884, Length=186, Percent_Identity=33.8709677419355, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI21729882, Length=186, Percent_Identity=33.8709677419355, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI21729880, Length=186, Percent_Identity=33.8709677419355, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI21729878, Length=186, Percent_Identity=33.8709677419355, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1790846, Length=211, Percent_Identity=32.2274881516588, Blast_Score=102, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322415, Length=253, Percent_Identity=26.0869565217391, Blast_Score=78, Evalue=1e-15,
Organism=Drosophila melanogaster, GI28573464, Length=155, Percent_Identity=31.6129032258064, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004143 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB [H]

EC number: =2.7.7.63 [H]

Molecular weight: Translated: 28410; Mature: 28410

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLRVLLTEYPNDPYLNVAMDEALLLHSKYVPILRIWRNEKSVVLGILSSISDEVNLEMI
CEEEEEEEECCCCCEEEEEHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCHHHH
KKYDVKLARRISGGGSVFHDMGNINYTFITSGQGGIDYLYGYLLRGTISAIENLVNDRVE
HHHHHHHHHHHCCCCCEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VYNETDIAFKGYKISGNAGYINEDKYLLHGTLLVSSNLDILHKVLIIPPKNYRKKDINMI
HCCCCCEEEEEEEEECCCCEECCCCEEEEEEEEEECCHHHEEEEEEECCCCCCHHHHHHH
KYRVSNLSNLVKGDITYDEAIEAFIRGFSSLLRCDDYFFDDISDEELKLAMRLANEKYSK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHCCCCH
KEFIYKR
HHCCCCC
>Mature Secondary Structure
MKLRVLLTEYPNDPYLNVAMDEALLLHSKYVPILRIWRNEKSVVLGILSSISDEVNLEMI
CEEEEEEEECCCCCEEEEEHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCHHHH
KKYDVKLARRISGGGSVFHDMGNINYTFITSGQGGIDYLYGYLLRGTISAIENLVNDRVE
HHHHHHHHHHHCCCCCEEECCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VYNETDIAFKGYKISGNAGYINEDKYLLHGTLLVSSNLDILHKVLIIPPKNYRKKDINMI
HCCCCCEEEEEEEEECCCCEECCCCEEEEEEEEEECCHHHEEEEEEECCCCCCHHHHHHH
KYRVSNLSNLVKGDITYDEAIEAFIRGFSSLLRCDDYFFDDISDEELKLAMRLANEKYSK
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCHHHCCCCCHHHHHHHHHHHHCCCCH
KEFIYKR
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11029001 [H]