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The map label for this gene is hyuB-3

Identifier: 15899644

GI number: 15899644

Start: 2677189

End: 2678730

Strand: Reverse

Name: hyuB-3

Synonym: SSO2934

Alternate gene names: NA

Gene position: 2678730-2677189 (Counterclockwise)

Preceding gene: 15899645

Following gene: 15899643

Centisome position: 89.52

GC content: 41.5

Gene sequence:

>1542_bases
ATGACAAGTTGGGAAATAATTTACAAAGCCTCTGAGTTCATTGCAGAGGAAATGGGCGTCATGCTAAAGAGGTCAGCTAT
GTCACCGAATATTAGGGAGAGGATGGATCATAGCTGTGCAATAACAGACGCTGAGGGTAATATTATAGCCCAAGCTGAAC
ACATACCTGTTCACCTAGGTTCTTTTAGCATTGGGGTTAAAAACACGCTGAACCAGATAGAAGAACTAGAAGAGGGGGAC
ATGATTGTTTTAAATGATCCTTACACATCCGGTACTCACCTAAATGACGTAATGGTTCTAGCCCCGGTTTACTATAATGG
CAATCTAATGGGATATGTTGTAAATAAGGCACATCAGGTTGATGTGGGTGGGCCGATGCCAGGTAGTCTAAATCCATATG
CAACTACAATCTACGAAGAGGGCTTCGTAATACCCCCAGTGAGGTTGATGAGGAAAGGAGAAATAAACAATGAGATTATT
AATATAATTAAGCATAACTTCAAGGTACCCGAAGTATCAATAGGTGATTTGAATGCACAGATTTCAGCAAACCTTAATGG
TATAGCTAGGATTAAGCAGATGTTTGATAAGTACGGTTACGATAACGTCCTTAACGCGTGGAAGACCTCTATGGAGTACG
GAAAGAAGTTGGCGTTATCCGAAATTTCAAAATGGGGTAATGGTATTGGTGAAGATGAGGATTATTTAGAAATAGGTGAT
GAATTAAAGAAAATAAGGCTAAAGGTAAAGGTTGATGAGAATGGCGTTTACGCTAATTTCGCTGGAACTGATACCCAGAT
GGAGGGACCTTTAAACGCAGTATATGGAGTTACGTTCTCAGCTGTTAGCTTTGTCATAAGGTCATTAATAGGGAAGGATA
TACCTACAAATGAGGGCTTTTATAGTCTAATTAAGGTTGATGCACCTAAGGGTACTATAGTTAATCCCAATAAGCCGGCT
GCAGTTGGAGGTGGGAATGTAGAGACTTCACAGAGGATTGCCGATGTTACCTTCAAGGCCTTATCTCACCTCATCAGCGT
TCCAGCCGCTGGTTCGGGTACTATGATGAACGTAATGATGGGTGGTATGTATAAGGGTAAGTATTGGGCCTATTATGAGA
CTATAGGCGGAGGCACTGGGGCAAGGCCAAATAAGGATGGCGTCTCAGCTGTTCAAGTTAATATGACTAATACTTTAAAT
ACTCCAATTGAGATTGCTGAGAGGTATTATCCAATAATGTTCACCATATATAGAATAAGGGAGAATAGTGGCGGAGTTGG
TAAGTACAAGGGAGGTGATGGTATAATTAGGGCATTTAGGGTTCTGGAAAAGACTAGGTTATCAATCATAGCGGAGAGGT
TTAGGGTTGCACCTTGGGGTTTGAAAGGTGGTGGAAACGGTAAGCCTGCAAAGGTTACGATAATAAGGGCTGATGGTAGG
AAGGAGGAGATGCCCAGTAAGTTCTCAACGATTTTAAATAAAGGTGATGAGATAATCATAGAAACTCCGGGCGGTGGAGG
ATATTACACACCTAATGAGTAA

Upstream 100 bases:

>100_bases
GAATAGGGGGGCCAGCAATAATTGAGGAATATAGTTCGACTACAGTTATAAAGGATGGTTGGAATGCAATTATAGATGAT
TCAATAGTATTGGTGAGAGA

Downstream 100 bases:

>100_bases
AAAATTCTAATTTCCCGTATAAAGATCCAATGATAATGTGCTTATACTTCGTCAGACTTTAAAGAGCAAGATTTATATTT
CATGAGTAATACTAAATTGT

Product: N-methylhydantoinase B (hyuB-3)

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: NA

Number of amino acids: Translated: 513; Mature: 512

Protein sequence:

>513_residues
MTSWEIIYKASEFIAEEMGVMLKRSAMSPNIRERMDHSCAITDAEGNIIAQAEHIPVHLGSFSIGVKNTLNQIEELEEGD
MIVLNDPYTSGTHLNDVMVLAPVYYNGNLMGYVVNKAHQVDVGGPMPGSLNPYATTIYEEGFVIPPVRLMRKGEINNEII
NIIKHNFKVPEVSIGDLNAQISANLNGIARIKQMFDKYGYDNVLNAWKTSMEYGKKLALSEISKWGNGIGEDEDYLEIGD
ELKKIRLKVKVDENGVYANFAGTDTQMEGPLNAVYGVTFSAVSFVIRSLIGKDIPTNEGFYSLIKVDAPKGTIVNPNKPA
AVGGGNVETSQRIADVTFKALSHLISVPAAGSGTMMNVMMGGMYKGKYWAYYETIGGGTGARPNKDGVSAVQVNMTNTLN
TPIEIAERYYPIMFTIYRIRENSGGVGKYKGGDGIIRAFRVLEKTRLSIIAERFRVAPWGLKGGGNGKPAKVTIIRADGR
KEEMPSKFSTILNKGDEIIIETPGGGGYYTPNE

Sequences:

>Translated_513_residues
MTSWEIIYKASEFIAEEMGVMLKRSAMSPNIRERMDHSCAITDAEGNIIAQAEHIPVHLGSFSIGVKNTLNQIEELEEGD
MIVLNDPYTSGTHLNDVMVLAPVYYNGNLMGYVVNKAHQVDVGGPMPGSLNPYATTIYEEGFVIPPVRLMRKGEINNEII
NIIKHNFKVPEVSIGDLNAQISANLNGIARIKQMFDKYGYDNVLNAWKTSMEYGKKLALSEISKWGNGIGEDEDYLEIGD
ELKKIRLKVKVDENGVYANFAGTDTQMEGPLNAVYGVTFSAVSFVIRSLIGKDIPTNEGFYSLIKVDAPKGTIVNPNKPA
AVGGGNVETSQRIADVTFKALSHLISVPAAGSGTMMNVMMGGMYKGKYWAYYETIGGGTGARPNKDGVSAVQVNMTNTLN
TPIEIAERYYPIMFTIYRIRENSGGVGKYKGGDGIIRAFRVLEKTRLSIIAERFRVAPWGLKGGGNGKPAKVTIIRADGR
KEEMPSKFSTILNKGDEIIIETPGGGGYYTPNE
>Mature_512_residues
TSWEIIYKASEFIAEEMGVMLKRSAMSPNIRERMDHSCAITDAEGNIIAQAEHIPVHLGSFSIGVKNTLNQIEELEEGDM
IVLNDPYTSGTHLNDVMVLAPVYYNGNLMGYVVNKAHQVDVGGPMPGSLNPYATTIYEEGFVIPPVRLMRKGEINNEIIN
IIKHNFKVPEVSIGDLNAQISANLNGIARIKQMFDKYGYDNVLNAWKTSMEYGKKLALSEISKWGNGIGEDEDYLEIGDE
LKKIRLKVKVDENGVYANFAGTDTQMEGPLNAVYGVTFSAVSFVIRSLIGKDIPTNEGFYSLIKVDAPKGTIVNPNKPAA
VGGGNVETSQRIADVTFKALSHLISVPAAGSGTMMNVMMGGMYKGKYWAYYETIGGGTGARPNKDGVSAVQVNMTNTLNT
PIEIAERYYPIMFTIYRIRENSGGVGKYKGGDGIIRAFRVLEKTRLSIIAERFRVAPWGLKGGGNGKPAKVTIIRADGRK
EEMPSKFSTILNKGDEIIIETPGGGGYYTPNE

Specific function: Unknown

COG id: COG0146

COG function: function code EQ; N-methylhydantoinase B/acetone carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=521, Percent_Identity=35.8925143953935, Blast_Score=305, Evalue=5e-83,
Organism=Caenorhabditis elegans, GI133901900, Length=520, Percent_Identity=37.6923076923077, Blast_Score=320, Evalue=1e-87,
Organism=Caenorhabditis elegans, GI133901902, Length=504, Percent_Identity=36.7063492063492, Blast_Score=298, Evalue=6e-81,
Organism=Saccharomyces cerevisiae, GI6322634, Length=526, Percent_Identity=36.1216730038023, Blast_Score=299, Evalue=6e-82,
Organism=Drosophila melanogaster, GI45550492, Length=522, Percent_Identity=34.4827586206897, Blast_Score=293, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003692 [H]

Pfam domain/function: PF02538 Hydantoinase_B [H]

EC number: 3.5.2.14

Molecular weight: Translated: 56143; Mature: 56012

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSWEIIYKASEFIAEEMGVMLKRSAMSPNIRERMDHSCAITDAEGNIIAQAEHIPVHLG
CCCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEECCCCEEEEECCCEEEEC
SFSIGVKNTLNQIEELEEGDMIVLNDPYTSGTHLNDVMVLAPVYYNGNLMGYVVNKAHQV
CEECCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEEEEECCCEEEEEECCCEEE
DVGGPMPGSLNPYATTIYEEGFVIPPVRLMRKGEINNEIINIIKHNFKVPEVSIGDLNAQ
ECCCCCCCCCCCCEEEEECCCEECCCHHHHHCCCCCHHHHHHHHHCCCCCCEEECCCCEE
ISANLNGIARIKQMFDKYGYDNVLNAWKTSMEYGKKLALSEISKWGNGIGEDEDYLEIGD
EECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
ELKKIRLKVKVDENGVYANFAGTDTQMEGPLNAVYGVTFSAVSFVIRSLIGKDIPTNEGF
HEEEEEEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
YSLIKVDAPKGTIVNPNKPAAVGGGNVETSQRIADVTFKALSHLISVPAAGSGTMMNVMM
EEEEEEECCCCCEECCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
GGMYKGKYWAYYETIGGGTGARPNKDGVSAVQVNMTNTLNTPIEIAERYYPIMFTIYRIR
CCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCEEEEEEEEE
ENSGGVGKYKGGDGIIRAFRVLEKTRLSIIAERFRVAPWGLKGGGNGKPAKVTIIRADGR
CCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCC
KEEMPSKFSTILNKGDEIIIETPGGGGYYTPNE
CHHCHHHHHHHHCCCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
TSWEIIYKASEFIAEEMGVMLKRSAMSPNIRERMDHSCAITDAEGNIIAQAEHIPVHLG
CCCEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCEEEEECCCCEEEEECCCEEEEC
SFSIGVKNTLNQIEELEEGDMIVLNDPYTSGTHLNDVMVLAPVYYNGNLMGYVVNKAHQV
CEECCHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCEEEEEEEEECCCEEEEEECCCEEE
DVGGPMPGSLNPYATTIYEEGFVIPPVRLMRKGEINNEIINIIKHNFKVPEVSIGDLNAQ
ECCCCCCCCCCCCEEEEECCCEECCCHHHHHCCCCCHHHHHHHHHCCCCCCEEECCCCEE
ISANLNGIARIKQMFDKYGYDNVLNAWKTSMEYGKKLALSEISKWGNGIGEDEDYLEIGD
EECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
ELKKIRLKVKVDENGVYANFAGTDTQMEGPLNAVYGVTFSAVSFVIRSLIGKDIPTNEGF
HEEEEEEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCE
YSLIKVDAPKGTIVNPNKPAAVGGGNVETSQRIADVTFKALSHLISVPAAGSGTMMNVMM
EEEEEEECCCCCEECCCCCCEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHH
GGMYKGKYWAYYETIGGGTGARPNKDGVSAVQVNMTNTLNTPIEIAERYYPIMFTIYRIR
CCCCCCCEEEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHCCEEEEEEEEE
ENSGGVGKYKGGDGIIRAFRVLEKTRLSIIAERFRVAPWGLKGGGNGKPAKVTIIRADGR
CCCCCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCC
KEEMPSKFSTILNKGDEIIIETPGGGGYYTPNE
CHHCHHHHHHHHCCCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]