Click here to switch to the map view.

The map label for this gene is shiA [H]

Identifier: 15899566

GI number: 15899566

Start: 2611121

End: 2612461

Strand: Reverse

Name: shiA [H]

Synonym: SSO2852

Alternate gene names: 15899566

Gene position: 2612461-2611121 (Counterclockwise)

Preceding gene: 15899568

Following gene: 15899562

Centisome position: 87.31

GC content: 39.08

Gene sequence:

>1341_bases
ATGGAGCCAGAAAAGTTTACATGGGATAGCGGGAAGGCAATAATATCCCAGTTTATAGGATTCCTTCTAGACTCCTATGA
TTTAACTATGATTTTAAGCATAGCTCCAGTTCTAGCTAAGGTAATTTTACCACCCGCAAGTCCACTCATAGCGACCTTTA
ATATCATTCTTTCCTACTCCTTAACAATAATATTCAGACCCTTAGGCTCAGCAATATTTGGAAATTTAGGAGATAAGATA
GGTAGGAGAGCTGACTTAATAATTACGGTATTGGGTTTAGGAATTACTAGTGCATTAACTTCAGCATTACCAACATATGC
ACAAGCTGGAATTCTTTCATTCGTTCTATTCGTTTTAATAAGAGTTGCTGTAGGAATCTTCGCTGGTGGAGAGTACTCAG
CAGGTCATCCATTTGCCATGGAGTGGACACCATATAAATGGAGAGGTTTCATTAGTGGACTAGTCCAAGGTGGTTTCTCA
TTTGGCGCAGCATTAGCTGCTGTTATAGAAGGTATCTTCATAGGAATTTATGGCGTATCCGGTGTTGAGGAGTTTGCGTG
GAGATATGTCTTTTTAACTGCCGTAATACCGGCTTTCATAGCCCTAGCAGTAAGACTCGCCATGAAGGAAACACCAGTTT
TTGAAGACGTTAAAAAGAAGAATCTGATTAGAAGGACACCACTGGCGGACTTGTTTAAAGCACCTTATAGAAGGGACTTC
TTACAAGTCATGGCATATATGACAGGCATGTTCTTCTTCGCCTATGCTCTATTTGCATTTGTGCCTACTATTTTGGAACA
TAGTCCTTCCACATTTAGTCTTGGAGAAGCAAACTACATATATAGTATCGGTACTTACGCTGCATTCGCAGGCGCGTTGA
TATTCGGCACTCTATCCCAATATGTTGGAAGAAGGAGGTTAACAATAATATGGAGTATAATTACCTTTGTCATTGCAATA
CCAATTTATTATGGGTTGATTACTGCGGCTATTGCTGGTAACGCTATAACTGCTATGTTATTCTCAATAATTATTGGTAT
AATAACGCAAGGACCATGGGGTATAATACCTATCTATTTATCTGAGAGATTTAAAGCATCAATGAGGGCTTCTGGAGTGG
GTTTCGGTTACTCCTCTGGAATTTTCCTTGGAGGTTGGTTCAGTATATACGTACCTCTGATGCATAACTACTTGTTTAAA
TCATTAGATACACCAAATAACGTATGGTTCTCTACTGCTGTATTATTAATGATTGGAGCTTTAATAGTTGGTATTGCACA
ATACTTCGGGCCAGAAACACTAGGAACTAGATTAGCTGAAGAACCACAAAAAACTACGTAG

Upstream 100 bases:

>100_bases
TTTAGGTGTGCAATGGTTCTTAGCTCTTTGTGAAAATTCAACAAGTCCACAAGGCATCTAAGAAGTTAATTTTTAATACA
ATAAGTTTAAAAAGGCTTCA

Downstream 100 bases:

>100_bases
AATAAAAATAAAAAGGGAAAGCTCTTTTTTTAGGGATTCTAATTTTTTTCAAAGACTTTTATCTTATCTTTTCTCACATA
CACTAATACTTCCTCACCAG

Product: proline/betaine transporter, putative

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MEPEKFTWDSGKAIISQFIGFLLDSYDLTMILSIAPVLAKVILPPASPLIATFNIILSYSLTIIFRPLGSAIFGNLGDKI
GRRADLIITVLGLGITSALTSALPTYAQAGILSFVLFVLIRVAVGIFAGGEYSAGHPFAMEWTPYKWRGFISGLVQGGFS
FGAALAAVIEGIFIGIYGVSGVEEFAWRYVFLTAVIPAFIALAVRLAMKETPVFEDVKKKNLIRRTPLADLFKAPYRRDF
LQVMAYMTGMFFFAYALFAFVPTILEHSPSTFSLGEANYIYSIGTYAAFAGALIFGTLSQYVGRRRLTIIWSIITFVIAI
PIYYGLITAAIAGNAITAMLFSIIIGIITQGPWGIIPIYLSERFKASMRASGVGFGYSSGIFLGGWFSIYVPLMHNYLFK
SLDTPNNVWFSTAVLLMIGALIVGIAQYFGPETLGTRLAEEPQKTT

Sequences:

>Translated_446_residues
MEPEKFTWDSGKAIISQFIGFLLDSYDLTMILSIAPVLAKVILPPASPLIATFNIILSYSLTIIFRPLGSAIFGNLGDKI
GRRADLIITVLGLGITSALTSALPTYAQAGILSFVLFVLIRVAVGIFAGGEYSAGHPFAMEWTPYKWRGFISGLVQGGFS
FGAALAAVIEGIFIGIYGVSGVEEFAWRYVFLTAVIPAFIALAVRLAMKETPVFEDVKKKNLIRRTPLADLFKAPYRRDF
LQVMAYMTGMFFFAYALFAFVPTILEHSPSTFSLGEANYIYSIGTYAAFAGALIFGTLSQYVGRRRLTIIWSIITFVIAI
PIYYGLITAAIAGNAITAMLFSIIIGIITQGPWGIIPIYLSERFKASMRASGVGFGYSSGIFLGGWFSIYVPLMHNYLFK
SLDTPNNVWFSTAVLLMIGALIVGIAQYFGPETLGTRLAEEPQKTT
>Mature_446_residues
MEPEKFTWDSGKAIISQFIGFLLDSYDLTMILSIAPVLAKVILPPASPLIATFNIILSYSLTIIFRPLGSAIFGNLGDKI
GRRADLIITVLGLGITSALTSALPTYAQAGILSFVLFVLIRVAVGIFAGGEYSAGHPFAMEWTPYKWRGFISGLVQGGFS
FGAALAAVIEGIFIGIYGVSGVEEFAWRYVFLTAVIPAFIALAVRLAMKETPVFEDVKKKNLIRRTPLADLFKAPYRRDF
LQVMAYMTGMFFFAYALFAFVPTILEHSPSTFSLGEANYIYSIGTYAAFAGALIFGTLSQYVGRRRLTIIWSIITFVIAI
PIYYGLITAAIAGNAITAMLFSIIIGIITQGPWGIIPIYLSERFKASMRASGVGFGYSSGIFLGGWFSIYVPLMHNYLFK
SLDTPNNVWFSTAVLLMIGALIVGIAQYFGPETLGTRLAEEPQKTT

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=423, Percent_Identity=28.3687943262411, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1790550, Length=387, Percent_Identity=27.390180878553, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1789941, Length=403, Percent_Identity=25.8064516129032, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI87082404, Length=371, Percent_Identity=22.1024258760108, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082231, Length=493, Percent_Identity=21.9066937119675, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1788942, Length=384, Percent_Identity=23.4375, Blast_Score=69, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6322632, Length=402, Percent_Identity=25.6218905472637, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 48706; Mature: 48706

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHCCHHHHHHHHCCCCCCC
>Mature Secondary Structure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HHHHHHHHHCCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]