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The map label for this gene is tenA-2 [H]

Identifier: 15899325

GI number: 15899325

Start: 2362611

End: 2363285

Strand: Reverse

Name: tenA-2 [H]

Synonym: SSO2598

Alternate gene names: 15899325

Gene position: 2363285-2362611 (Counterclockwise)

Preceding gene: 15899329

Following gene: 15899324

Centisome position: 78.98

GC content: 32.59

Gene sequence:

>675_bases
ATGATGGAAAACAGTGAAAAGTTATGGAATTTTATTTCGGATATTTATTCTTCAATCCTTAGACACCCATTTATTCTTGA
ATTGGTTGAAGGAATTCTAAGCAGAGATAAATTCAAGTACTATATAATCCAGGATTATCTGTATTTAAGGGAATTCTCTA
AGGCATTGGCTTTATTAGCGGCAAAGGCAGAAGATGAGGAACAAACATTGTTGTTTACGACGCACATACAAGATGCCATT
AAGGTAGAAAAGGCGTTACATAAATTTTACATAAAGGAATTTAATTTAGATATAGAAGATTATGAGATGAGCCCAACTAA
TCTAGCCTATACATCTTACTTGCTTGCAGTAGCTTATTCAAGACCTTTTAATGAGGTAATATCTGCAGTATTGCCTTGCT
ATTGGATATATATGAAGGTAGGAAAGGAATTGCTTAAACAAGGATCTAAAGATAAATATTATCAAAAGTGGATAGAAACG
TACGGTGGAGAAGAATATGAAAAGGGAGTTAGAGCAGTATTAGATATTGTGAATAGCTTGAAAGTTAGTGAAGAGGAATT
TAACAAGATGAAGATTCATTTTAGAACTGCATCTATATATGAATACATGTTTTGGGACTCTGCGTATAGATTAGAGAGGT
TTCCTTTTCCTACAGAAAAAAATAAAGGAGTGTAA

Upstream 100 bases:

>100_bases
AAAGATGATTTTTAATAAATATGTTGATAACTCCTCAACTTAACTGAGAATACCTTGTAAGCCTTAAAATTTAAATAACA
GGCATGTTTCATTTCCTTAT

Downstream 100 bases:

>100_bases
CTAACTTACCTTTTATGGATAAACTGCTACTTCATGTAGGCCCTACTACTATCAAGGAAGATGTTTTAGTTGCGGGGCTT
GAAAATAACGTTGGCTTTAC

Product: transcriptional activator (tenA-2)

Products: NA

Alternate protein names: Thiaminase II; Transcriptional activator tenA [H]

Number of amino acids: Translated: 224; Mature: 224

Protein sequence:

>224_residues
MMENSEKLWNFISDIYSSILRHPFILELVEGILSRDKFKYYIIQDYLYLREFSKALALLAAKAEDEEQTLLFTTHIQDAI
KVEKALHKFYIKEFNLDIEDYEMSPTNLAYTSYLLAVAYSRPFNEVISAVLPCYWIYMKVGKELLKQGSKDKYYQKWIET
YGGEEYEKGVRAVLDIVNSLKVSEEEFNKMKIHFRTASIYEYMFWDSAYRLERFPFPTEKNKGV

Sequences:

>Translated_224_residues
MMENSEKLWNFISDIYSSILRHPFILELVEGILSRDKFKYYIIQDYLYLREFSKALALLAAKAEDEEQTLLFTTHIQDAI
KVEKALHKFYIKEFNLDIEDYEMSPTNLAYTSYLLAVAYSRPFNEVISAVLPCYWIYMKVGKELLKQGSKDKYYQKWIET
YGGEEYEKGVRAVLDIVNSLKVSEEEFNKMKIHFRTASIYEYMFWDSAYRLERFPFPTEKNKGV
>Mature_224_residues
MMENSEKLWNFISDIYSSILRHPFILELVEGILSRDKFKYYIIQDYLYLREFSKALALLAAKAEDEEQTLLFTTHIQDAI
KVEKALHKFYIKEFNLDIEDYEMSPTNLAYTSYLLAVAYSRPFNEVISAVLPCYWIYMKVGKELLKQGSKDKYYQKWIET
YGGEEYEKGVRAVLDIVNSLKVSEEEFNKMKIHFRTASIYEYMFWDSAYRLERFPFPTEKNKGV

Specific function: Stimulates the production of several extracellular degradative enzymes at the transcriptional level, probably by interfering with degS and degU, which are required for this effect. Is not an essential protein, but affects the sporulation frequency [H]

COG id: COG0819

COG function: function code K; Putative transcription activator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiaminase-2 family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6324517, Length=212, Percent_Identity=25.4716981132075, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016084
- InterPro:   IPR004305 [H]

Pfam domain/function: PF03070 TENA_THI-4 [H]

EC number: =3.5.99.2 [H]

Molecular weight: Translated: 26669; Mature: 26669

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMENSEKLWNFISDIYSSILRHPFILELVEGILSRDKFKYYIIQDYLYLREFSKALALLA
CCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
AKAEDEEQTLLFTTHIQDAIKVEKALHKFYIKEFNLDIEDYEMSPTNLAYTSYLLAVAYS
HCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHC
RPFNEVISAVLPCYWIYMKVGKELLKQGSKDKYYQKWIETYGGEEYEKGVRAVLDIVNSL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
KVSEEEFNKMKIHFRTASIYEYMFWDSAYRLERFPFPTEKNKGV
CCCHHHHHHEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MMENSEKLWNFISDIYSSILRHPFILELVEGILSRDKFKYYIIQDYLYLREFSKALALLA
CCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
AKAEDEEQTLLFTTHIQDAIKVEKALHKFYIKEFNLDIEDYEMSPTNLAYTSYLLAVAYS
HCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHC
RPFNEVISAVLPCYWIYMKVGKELLKQGSKDKYYQKWIETYGGEEYEKGVRAVLDIVNSL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
KVSEEEFNKMKIHFRTASIYEYMFWDSAYRLERFPFPTEKNKGV
CCCHHHHHHEEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1898926; 9384377 [H]