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The map label for this gene is leuD [H]

Identifier: 15899212

GI number: 15899212

Start: 2240677

End: 2241174

Strand: Reverse

Name: leuD [H]

Synonym: SSO2470

Alternate gene names: 15899212

Gene position: 2241174-2240677 (Counterclockwise)

Preceding gene: 15899213

Following gene: 15899211

Centisome position: 74.9

GC content: 38.76

Gene sequence:

>498_bases
ATGATAATAGAAGGTCCAGTAATTAAGTTTGGTGATAAAATAGATACTGATATAATAATTCCAGCTAGGTACTTAAAATA
TACTGACCCACAGTATTTAGCACAGCACGTTATGGAACCATTAGACCCAGAGTTTTACAAGAAGGCATCAAAGGGTGTTA
TAATAGTTGCGGGTAAAGTATTTGGAATGGGATCCTCAAGAGAACAAGCTGCCATAGCCCTAAAGGCTGCTGGAGTTAAG
GCAGTAGTTGCTGAGTCATTCGCTAGAATATTCTATAGGAATGCGATAAATAATGGTTTACCAGTCATAACGTTGCCTAA
TTCCACGAAGGAGATAGATGAAAACAGTTACGTCAAAATTGACGTGGAGACTGGTGAAATCTTGGTTGGTAATAAGGTGC
TTAAGGGAAAGGGCATAACAGGGATGGCACTAGAGATTCTACAAGCTGGAGGAATAATGGAATATCTAAAGAAGATGCAG
ACAGTAAACCGAAACTAA

Upstream 100 bases:

>100_bases
GAGAATGGGAAGTAATGAGGCTAAAGTTTACTTATCTGGTCCCGCTGTTGCTGCAATTAGCGCATTAGAAGGTAAGATTA
CAGATCCCAGGGTGATCTGA

Downstream 100 bases:

>100_bases
AAAGTTTATTTTTTAGTTTTTAATACTAATATTGTGAAGTGGATTATTATCGGTTTGGTCTCCCTAATGCTTACAATCGT
TGATTATGGGATTGGAATAG

Product: 3-isopropylmalate dehydratase small subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase [H]

Number of amino acids: Translated: 165; Mature: 165

Protein sequence:

>165_residues
MIIEGPVIKFGDKIDTDIIIPARYLKYTDPQYLAQHVMEPLDPEFYKKASKGVIIVAGKVFGMGSSREQAAIALKAAGVK
AVVAESFARIFYRNAINNGLPVITLPNSTKEIDENSYVKIDVETGEILVGNKVLKGKGITGMALEILQAGGIMEYLKKMQ
TVNRN

Sequences:

>Translated_165_residues
MIIEGPVIKFGDKIDTDIIIPARYLKYTDPQYLAQHVMEPLDPEFYKKASKGVIIVAGKVFGMGSSREQAAIALKAAGVK
AVVAESFARIFYRNAINNGLPVITLPNSTKEIDENSYVKIDVETGEILVGNKVLKGKGITGMALEILQAGGIMEYLKKMQ
TVNRN
>Mature_165_residues
MIIEGPVIKFGDKIDTDIIIPARYLKYTDPQYLAQHVMEPLDPEFYKKASKGVIIVAGKVFGMGSSREQAAIALKAAGVK
AVVAESFARIFYRNAINNGLPVITLPNSTKEIDENSYVKIDVETGEILVGNKVLKGKGITGMALEILQAGGIMEYLKKMQ
TVNRN

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG0066

COG function: function code E; 3-isopropylmalate dehydratase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leuD family. LeuD type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786258, Length=145, Percent_Identity=33.1034482758621, Blast_Score=72, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6320440, Length=174, Percent_Identity=36.7816091954023, Blast_Score=87, Evalue=9e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012305
- InterPro:   IPR015937
- InterPro:   IPR015928
- InterPro:   IPR000573
- InterPro:   IPR011827 [H]

Pfam domain/function: PF00694 Aconitase_C [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 18046; Mature: 18046

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIEGPVIKFGDKIDTDIIIPARYLKYTDPQYLAQHVMEPLDPEFYKKASKGVIIVAGKV
CCCCCCEEECCCCCCCEEEECHHHHCCCCHHHHHHHHHCCCCHHHHHHHCCCEEEEEEEE
FGMGSSREQAAIALKAAGVKAVVAESFARIFYRNAINNGLPVITLPNSTKEIDENSYVKI
ECCCCCCCHHEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEEEE
DVETGEILVGNKVLKGKGITGMALEILQAGGIMEYLKKMQTVNRN
EECCCCEEECCEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MIIEGPVIKFGDKIDTDIIIPARYLKYTDPQYLAQHVMEPLDPEFYKKASKGVIIVAGKV
CCCCCCEEECCCCCCCEEEECHHHHCCCCHHHHHHHHHCCCCHHHHHHHCCCEEEEEEEE
FGMGSSREQAAIALKAAGVKAVVAESFARIFYRNAINNGLPVITLPNSTKEIDENSYVKI
ECCCCCCCHHEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCEEEE
DVETGEILVGNKVLKGKGITGMALEILQAGGIMEYLKKMQTVNRN
EECCCCEEECCEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA