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The map label for this gene is leuC

Identifier: 15899213

GI number: 15899213

Start: 2241175

End: 2242425

Strand: Reverse

Name: leuC

Synonym: SSO2471

Alternate gene names: 15899213

Gene position: 2242425-2241175 (Counterclockwise)

Preceding gene: 15899214

Following gene: 15899212

Centisome position: 74.94

GC content: 38.85

Gene sequence:

>1251_bases
TTGATGTCGGCAAAAACGTTAACTGAGAAAATTTTAAGCAGAGCATCGGGAAAGGATGTCTCGCCTGGAGACGTTATAGA
GGCTAAAGTAGACTTAGTAGCATTTCATGACTTAACTGGTTATCACGTGATTGAGGTAATGGAAAAGGCTAACATGATTA
AAGTTTTCGATAAAAGTAAATTAGTAATAGCTTTTGATCATTTAGCTCCACCTCCAGATGTAAGGAGTGCAGAAATTCAA
GGCTATATAAGAAAGTTCGTGAAATCGGAAGGTATTCCTAATTTTTATGATATAAATTACGGAATTTTACATGAAGTCAT
GATAGAGCAATACGCCAACCCTGGACAAGTTATTTTAGCAGCTGATAGTCACACAACCACATCCGGTGCTGTAGGAGCAT
TTGCTCAAGGTATGGGAGCTAGTGATATTGCAGCCGCTTTAATAACTGGTAAAACTTGGCTAGTAGTGCCACAACCATTT
AAAGTAGTTTTAGAAGGAAAACCTGCTAAATGGATAACTGGGAAGGACGTTGCACTAAAATTGCTAGGAGACTTTAAGGC
AGATTACTTTAATGGAATGACGTTAGAAATCTTCGTCAAGGATCCGTTAAGTTTTCCAATGGATTATAGGGCAACAGTAT
CAAATATGGGAATTGAAATGAATGCTGATGCATTAATGTTCATACCAGATCAAGAGACTAAGAGATACATCAAAGAGATG
AGAGGATACGAACCAGAACTTGTTACACCAGATAACGGCGCTAAATACGTTGATGAATACACTATTCAACTAGACGAAAT
GGAACCACTAGTTGCTGCTCCACATAGTGTAGATAATGTTAAGGTCGTAAATGAGTTAGAAGGTACTCCAGTAGATCAAG
TTTACATAGGCTCTTGTACAAATGGTAGGTTGAGCGATTTCGAAATAGCTGCTAAAATAATGAAAGGAAAGAAGGTCAAG
AGTAGGTGCATCGCCATCCCTGCGTCTTACAGAATGTTTAAGGAGGCTTTAGAAAGAGGTTATATACAAACATTAGTTGA
TGCTGGTTGTATTGTAACGTATGGAACTTGTGGACCATGCCTAGGTGGTCATTTCGGTATAGCTGGTCCAGGTGAGAATA
TTGTATCAACAAGTTCCAGAAACTTTAAAGGGAGAATGGGAAGTAATGAGGCTAAAGTTTACTTATCTGGTCCCGCTGTT
GCTGCAATTAGCGCATTAGAAGGTAAGATTACAGATCCCAGGGTGATCTGA

Upstream 100 bases:

>100_bases
GGAAATCTAAAATAAAATAGGTTTCTTTTTCGGATTTAGTACGATGTAAATGTAATTTCTTCGTAAGAAATGCTTATTTA
TTAATAATTTTAACTCCTTA

Downstream 100 bases:

>100_bases
ATGATAATAGAAGGTCCAGTAATTAAGTTTGGTGATAAAATAGATACTGATATAATAATTCCAGCTAGGTACTTAAAATA
TACTGACCCACAGTATTTAG

Product: 3-isopropylmalate dehydratase large subunit

Products: NA

Alternate protein names: Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase

Number of amino acids: Translated: 416; Mature: 416

Protein sequence:

>416_residues
MMSAKTLTEKILSRASGKDVSPGDVIEAKVDLVAFHDLTGYHVIEVMEKANMIKVFDKSKLVIAFDHLAPPPDVRSAEIQ
GYIRKFVKSEGIPNFYDINYGILHEVMIEQYANPGQVILAADSHTTTSGAVGAFAQGMGASDIAAALITGKTWLVVPQPF
KVVLEGKPAKWITGKDVALKLLGDFKADYFNGMTLEIFVKDPLSFPMDYRATVSNMGIEMNADALMFIPDQETKRYIKEM
RGYEPELVTPDNGAKYVDEYTIQLDEMEPLVAAPHSVDNVKVVNELEGTPVDQVYIGSCTNGRLSDFEIAAKIMKGKKVK
SRCIAIPASYRMFKEALERGYIQTLVDAGCIVTYGTCGPCLGGHFGIAGPGENIVSTSSRNFKGRMGSNEAKVYLSGPAV
AAISALEGKITDPRVI

Sequences:

>Translated_416_residues
MMSAKTLTEKILSRASGKDVSPGDVIEAKVDLVAFHDLTGYHVIEVMEKANMIKVFDKSKLVIAFDHLAPPPDVRSAEIQ
GYIRKFVKSEGIPNFYDINYGILHEVMIEQYANPGQVILAADSHTTTSGAVGAFAQGMGASDIAAALITGKTWLVVPQPF
KVVLEGKPAKWITGKDVALKLLGDFKADYFNGMTLEIFVKDPLSFPMDYRATVSNMGIEMNADALMFIPDQETKRYIKEM
RGYEPELVTPDNGAKYVDEYTIQLDEMEPLVAAPHSVDNVKVVNELEGTPVDQVYIGSCTNGRLSDFEIAAKIMKGKKVK
SRCIAIPASYRMFKEALERGYIQTLVDAGCIVTYGTCGPCLGGHFGIAGPGENIVSTSSRNFKGRMGSNEAKVYLSGPAV
AAISALEGKITDPRVI
>Mature_416_residues
MMSAKTLTEKILSRASGKDVSPGDVIEAKVDLVAFHDLTGYHVIEVMEKANMIKVFDKSKLVIAFDHLAPPPDVRSAEIQ
GYIRKFVKSEGIPNFYDINYGILHEVMIEQYANPGQVILAADSHTTTSGAVGAFAQGMGASDIAAALITGKTWLVVPQPF
KVVLEGKPAKWITGKDVALKLLGDFKADYFNGMTLEIFVKDPLSFPMDYRATVSNMGIEMNADALMFIPDQETKRYIKEM
RGYEPELVTPDNGAKYVDEYTIQLDEMEPLVAAPHSVDNVKVVNELEGTPVDQVYIGSCTNGRLSDFEIAAKIMKGKKVK
SRCIAIPASYRMFKEALERGYIQTLVDAGCIVTYGTCGPCLGGHFGIAGPGENIVSTSSRNFKGRMGSNEAKVYLSGPAV
AAISALEGKITDPRVI

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate

COG id: COG0065

COG function: function code E; 3-isopropylmalate dehydratase large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4501867, Length=447, Percent_Identity=29.9776286353468, Blast_Score=132, Evalue=5e-31,
Organism=Escherichia coli, GI1786259, Length=371, Percent_Identity=30.9973045822102, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI87081781, Length=434, Percent_Identity=24.4239631336406, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI2367097, Length=450, Percent_Identity=26.2222222222222, Blast_Score=85, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI25149337, Length=447, Percent_Identity=27.9642058165548, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI32564738, Length=368, Percent_Identity=28.804347826087, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI25149342, Length=284, Percent_Identity=29.2253521126761, Blast_Score=94, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6320440, Length=450, Percent_Identity=31.5555555555556, Blast_Score=174, Evalue=3e-44,
Organism=Saccharomyces cerevisiae, GI6323335, Length=447, Percent_Identity=27.9642058165548, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6321429, Length=360, Percent_Identity=29.7222222222222, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6322261, Length=365, Percent_Identity=28.2191780821918, Blast_Score=115, Evalue=9e-27,
Organism=Drosophila melanogaster, GI281365315, Length=447, Percent_Identity=27.2930648769575, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI17864292, Length=447, Percent_Identity=27.2930648769575, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI28571643, Length=446, Percent_Identity=27.5784753363229, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI161076999, Length=344, Percent_Identity=29.0697674418605, Blast_Score=110, Evalue=1e-24,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEUC_SULSO (Q97VY2)

Other databases:

- EMBL:   AE006641
- PIR:   A99419
- RefSeq:   NP_343818.1
- ProteinModelPortal:   Q97VY2
- SMR:   Q97VY2
- GeneID:   1453935
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO2471
- NMPDR:   fig|273057.1.peg.2241
- HOGENOM:   HBG330745
- OMA:   GADALMF
- PhylomeDB:   Q97VY2
- ProtClustDB:   PRK00402
- BioCyc:   SSOL273057:SSO2471-MONOMER
- BRENDA:   4.2.1.33
- HAMAP:   MF_01027
- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251
- Gene3D:   G3DSA:3.30.499.10
- Gene3D:   G3DSA:3.40.1060.10
- PANTHER:   PTHR11670
- PANTHER:   PTHR11670:SF6
- PRINTS:   PR00415
- TIGRFAMs:   TIGR01343
- TIGRFAMs:   TIGR02086

Pfam domain/function: PF00330 Aconitase; SSF53732 Aconitase_N

EC number: =4.2.1.33

Molecular weight: Translated: 45254; Mature: 45254

Theoretical pI: Translated: 5.72; Mature: 5.72

Prosite motif: PS00450 ACONITASE_1; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSAKTLTEKILSRASGKDVSPGDVIEAKVDLVAFHDLTGYHVIEVMEKANMIKVFDKSK
CCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCCHHHHHHHHHCCEEEEECCCE
LVIAFDHLAPPPDVRSAEIQGYIRKFVKSEGIPNFYDINYGILHEVMIEQYANPGQVILA
EEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCCCEEEE
ADSHTTTSGAVGAFAQGMGASDIAAALITGKTWLVVPQPFKVVLEGKPAKWITGKDVALK
ECCCCCCCCCHHHHHCCCCHHHHHHHEECCCEEEEECCCEEEEEECCCCCEECCCHHHHH
LLGDFKADYFNGMTLEIFVKDPLSFPMDYRATVSNMGIEMNADALMFIPDQETKRYIKEM
HHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHCCEEECCCEEEEECCHHHHHHHHHH
RGYEPELVTPDNGAKYVDEYTIQLDEMEPLVAAPHSVDNVKVVNELEGTPVDQVYIGSCT
CCCCCCEECCCCCCEEEEEEEEEECCCCCHHCCCCCCCCEEEEHHCCCCCCCEEEEECCC
NGRLSDFEIAAKIMKGKKVKSRCIAIPASYRMFKEALERGYIQTLVDAGCIVTYGTCGPC
CCCCCHHHHHHHHHHCCCHHHHEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
LGGHFGIAGPGENIVSTSSRNFKGRMGSNEAKVYLSGPAVAAISALEGKITDPRVI
CCCCCCCCCCCCCCEECCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MMSAKTLTEKILSRASGKDVSPGDVIEAKVDLVAFHDLTGYHVIEVMEKANMIKVFDKSK
CCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEECCCCHHHHHHHHHCCEEEEECCCE
LVIAFDHLAPPPDVRSAEIQGYIRKFVKSEGIPNFYDINYGILHEVMIEQYANPGQVILA
EEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHCCCCCCEEEE
ADSHTTTSGAVGAFAQGMGASDIAAALITGKTWLVVPQPFKVVLEGKPAKWITGKDVALK
ECCCCCCCCCHHHHHCCCCHHHHHHHEECCCEEEEECCCEEEEEECCCCCEECCCHHHHH
LLGDFKADYFNGMTLEIFVKDPLSFPMDYRATVSNMGIEMNADALMFIPDQETKRYIKEM
HHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHCCEEECCCEEEEECCHHHHHHHHHH
RGYEPELVTPDNGAKYVDEYTIQLDEMEPLVAAPHSVDNVKVVNELEGTPVDQVYIGSCT
CCCCCCEECCCCCCEEEEEEEEEECCCCCHHCCCCCCCCEEEEHHCCCCCCCEEEEECCC
NGRLSDFEIAAKIMKGKKVKSRCIAIPASYRMFKEALERGYIQTLVDAGCIVTYGTCGPC
CCCCCHHHHHHHHHHCCCHHHHEEECCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCC
LGGHFGIAGPGENIVSTSSRNFKGRMGSNEAKVYLSGPAVAAISALEGKITDPRVI
CCCCCCCCCCCCCCEECCCCCCCCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726