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The map label for this gene is pycB [H]

Identifier: 15899208

GI number: 15899208

Start: 2235498

End: 2236058

Strand: Reverse

Name: pycB [H]

Synonym: SSO2464

Alternate gene names: 15899208

Gene position: 2236058-2235498 (Counterclockwise)

Preceding gene: 15899209

Following gene: 15899207

Centisome position: 74.73

GC content: 33.16

Gene sequence:

>561_bases
TTGGAAAACTTATGGTATCATATTCCAATCCTCTCCAAGGGTGTTGTGGTAATGAGATTATTTAGACTTTATAGCGAATT
GGGAGATATGTATTTAGTATCTTATGAACAGAGCAACAACAGTCTTGATAAAATAAAAATAGGAGATAAAAATTATGAGG
TAAAATATCTAGGATCAGGCAATAGGGAAAATGAGTATTTATTTGAGATTAATGGGAAAAAATATTATGTTTTCATAGAG
TCTGATGGTACTTTGATATTTAACCATCAGGATTTCTTAAGGTTAGATAAGGTAACTGAAATTCCCATAAAAGGAGAAGA
AAGAGTTGAGGAAATAATTAGAGGAAAGGAAGGAGAAATTGTATCTCCATTATTTGGAAGGGTAGTTAAGATTAGAGTAA
AGGAAGGGGATGCAGTAAATAAGGGTCAACCTTTGTTATCCATTGAGGCAATGAAGGCAGAAACGGTAATCTCATCGCCA
ATAGGTGGGATCGTGCAAAAAATTCTGATCAAGGAAGGACAAGGTGTAAAGAAGGGAGATATTTTAATTGTTATAAAATA
G

Upstream 100 bases:

>100_bases
TACAAATTGCAATAGCCATTTCGATTTATAATAGGGGATTGATGAAGAAGAAAAAAACTGAACAGAAAGTACCCATCTCA
ACTAGTAATGGTAAAAGTAA

Downstream 100 bases:

>100_bases
TTTTACAGTTATGAGGTGAGATAAATGGCTCTATATGAAAAGCCTTCAATGGATAAATTAATTGAAGATTTGAAAATATT
AAAGGAAAAGGTATATAAGG

Product: biotin carboxyl carrier protein of propionyl-CoA carboxylase beta subunit

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MENLWYHIPILSKGVVVMRLFRLYSELGDMYLVSYEQSNNSLDKIKIGDKNYEVKYLGSGNRENEYLFEINGKKYYVFIE
SDGTLIFNHQDFLRLDKVTEIPIKGEERVEEIIRGKEGEIVSPLFGRVVKIRVKEGDAVNKGQPLLSIEAMKAETVISSP
IGGIVQKILIKEGQGVKKGDILIVIK

Sequences:

>Translated_186_residues
MENLWYHIPILSKGVVVMRLFRLYSELGDMYLVSYEQSNNSLDKIKIGDKNYEVKYLGSGNRENEYLFEINGKKYYVFIE
SDGTLIFNHQDFLRLDKVTEIPIKGEERVEEIIRGKEGEIVSPLFGRVVKIRVKEGDAVNKGQPLLSIEAMKAETVISSP
IGGIVQKILIKEGQGVKKGDILIVIK
>Mature_186_residues
MENLWYHIPILSKGVVVMRLFRLYSELGDMYLVSYEQSNNSLDKIKIGDKNYEVKYLGSGNRENEYLFEINGKKYYVFIE
SDGTLIFNHQDFLRLDKVTEIPIKGEERVEEIIRGKEGEIVSPLFGRVVKIRVKEGDAVNKGQPLLSIEAMKAETVISSP
IGGIVQKILIKEGQGVKKGDILIVIK

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG0511

COG function: function code I; Biotin carboxyl carrier protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI17562816, Length=124, Percent_Identity=32.258064516129, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24652212, Length=110, Percent_Identity=36.3636363636364, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24652210, Length=110, Percent_Identity=36.3636363636364, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24652214, Length=110, Percent_Identity=36.3636363636364, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI19921944, Length=110, Percent_Identity=36.3636363636364, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24652216, Length=110, Percent_Identity=36.3636363636364, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI281363050, Length=110, Percent_Identity=36.3636363636364, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24652224, Length=110, Percent_Identity=36.3636363636364, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24652222, Length=110, Percent_Identity=36.3636363636364, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24652220, Length=110, Percent_Identity=36.3636363636364, Blast_Score=69, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24652218, Length=110, Percent_Identity=36.3636363636364, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 21167; Mature: 21167

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00188 BIOTIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENLWYHIPILSKGVVVMRLFRLYSELGDMYLVSYEQSNNSLDKIKIGDKNYEVKYLGSG
CCCEEEECCCHHCCHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCCCEEEEEECCC
NRENEYLFEINGKKYYVFIESDGTLIFNHQDFLRLDKVTEIPIKGEERVEEIIRGKEGEI
CCCCCEEEEECCCEEEEEEECCCEEEECCHHHEEECCEEECCCCCHHHHHHHHCCCCCCE
VSPLFGRVVKIRVKEGDAVNKGQPLLSIEAMKAETVISSPIGGIVQKILIKEGQGVKKGD
ECCCCCEEEEEEEECCCCCCCCCCEEEEEEHHHHHHHCCCHHHHHHHHHHHCCCCCCCCC
ILIVIK
EEEEEC
>Mature Secondary Structure
MENLWYHIPILSKGVVVMRLFRLYSELGDMYLVSYEQSNNSLDKIKIGDKNYEVKYLGSG
CCCEEEECCCHHCCHHHHHHHHHHHHCCCEEEEEEECCCCCEEEEEECCCCEEEEEECCC
NRENEYLFEINGKKYYVFIESDGTLIFNHQDFLRLDKVTEIPIKGEERVEEIIRGKEGEI
CCCCCEEEEECCCEEEEEEECCCEEEECCHHHEEECCEEECCCCCHHHHHHHHCCCCCCE
VSPLFGRVVKIRVKEGDAVNKGQPLLSIEAMKAETVISSPIGGIVQKILIKEGQGVKKGD
ECCCCCEEEEEEEECCCCCCCCCCEEEEEEHHHHHHHCCCHHHHHHHHHHHCCCCCCCCC
ILIVIK
EEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]