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The map label for this gene is nfi [H]

Identifier: 15899199

GI number: 15899199

Start: 2225443

End: 2226039

Strand: Direct

Name: nfi [H]

Synonym: SSO2454

Alternate gene names: 15899199

Gene position: 2225443-2226039 (Clockwise)

Preceding gene: 15899198

Following gene: 15899200

Centisome position: 74.37

GC content: 35.01

Gene sequence:

>597_bases
TTGGTTGAAAAACATCTACTCGAATTCTTAGAGAAATTACAATTCCTTATCTCAAAAAACGTTAAAATAAGTCATTACGG
AATTGAGAACGTTAAGAAAATCTGTGGGGTTGATATTGCATATAAGGGTAATCTAGGATTCTCAGTAGGTGTAAGTATGG
ATATTAATAGTGGAGATTATAATTACAAATCTTATGTTGGTGAAGTGAATTTCCCATATATTCCAGGTTTCCTTTTCATG
AGGGAAGCTCCATTAATGATAAAAGCAATAGAGGGTTTGGATTGTCACCTTCTTTTGGTTGATGGTCATGGGATAGCTCA
TCCTAGGAAAAGCGGAATTGCTGCAGTTATAGGCGTTCTCCTAGACTTCCCAACAATTGGGGTAGCGAAATCTAGGCTTA
CGGGTGATTTGGTTAATGAAAGTGAAATTACCTATGTATATCTAAATGGAGAGAAAGTAGGGGTTAAATTCGGTAGATAT
TTTTATAGTCCAGGAAATAAAGTCGACTTACAAGATTGCATTGAACTAGGTAAGAGGGGTTATCCTAAAGTTCTAAAAAT
AGCTGATATGCTCACTAAGAAGATAAAAAAGGAATAA

Upstream 100 bases:

>100_bases
TAGAATCTGCTATTGACGAAGTTAGATTGGTAAGACCTGGTGCAGTACAAACATATGAGCAAGAGATGTTCTTATTACGC
GTGGAAGGGATGAGAAAGAG

Downstream 100 bases:

>100_bases
AACTGTGCGTGCAGTAAATTTAATTATGAAAATTGAAGTACCATCTATTCCCGAGCATAAAGAGGTAATATTAGATCCTA
CTAATACAGCATTAATAATT

Product: endonuclease V (nfi)

Products: NA

Alternate protein names: Deoxyinosine 3'endonuclease; Deoxyribonuclease V; DNase V [H]

Number of amino acids: Translated: 198; Mature: 198

Protein sequence:

>198_residues
MVEKHLLEFLEKLQFLISKNVKISHYGIENVKKICGVDIAYKGNLGFSVGVSMDINSGDYNYKSYVGEVNFPYIPGFLFM
REAPLMIKAIEGLDCHLLLVDGHGIAHPRKSGIAAVIGVLLDFPTIGVAKSRLTGDLVNESEITYVYLNGEKVGVKFGRY
FYSPGNKVDLQDCIELGKRGYPKVLKIADMLTKKIKKE

Sequences:

>Translated_198_residues
MVEKHLLEFLEKLQFLISKNVKISHYGIENVKKICGVDIAYKGNLGFSVGVSMDINSGDYNYKSYVGEVNFPYIPGFLFM
REAPLMIKAIEGLDCHLLLVDGHGIAHPRKSGIAAVIGVLLDFPTIGVAKSRLTGDLVNESEITYVYLNGEKVGVKFGRY
FYSPGNKVDLQDCIELGKRGYPKVLKIADMLTKKIKKE
>Mature_198_residues
MVEKHLLEFLEKLQFLISKNVKISHYGIENVKKICGVDIAYKGNLGFSVGVSMDINSGDYNYKSYVGEVNFPYIPGFLFM
REAPLMIKAIEGLDCHLLLVDGHGIAHPRKSGIAAVIGVLLDFPTIGVAKSRLTGDLVNESEITYVYLNGEKVGVKFGRY
FYSPGNKVDLQDCIELGKRGYPKVLKIADMLTKKIKKE

Specific function: Selectively cleaves double-stranded DNA at the second phosphodiester bond 3' to a deoxyinosine leaving behind the intact lesion on the nicked DNA. Acts in DNA repair [H]

COG id: COG1515

COG function: function code L; Deoxyinosine 3'endonuclease (endonuclease V)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the endonuclease V family [H]

Homologues:

Organism=Escherichia coli, GI87082357, Length=181, Percent_Identity=32.5966850828729, Blast_Score=79, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007581 [H]

Pfam domain/function: PF04493 Endonuclease_5 [H]

EC number: =3.1.21.7 [H]

Molecular weight: Translated: 22051; Mature: 22051

Theoretical pI: Translated: 8.97; Mature: 8.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEKHLLEFLEKLQFLISKNVKISHYGIENVKKICGVDIAYKGNLGFSVGVSMDINSGDY
CCHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCEEEECCCCEEEEEEEEECCCCC
NYKSYVGEVNFPYIPGFLFMREAPLMIKAIEGLDCHLLLVDGHGIAHPRKSGIAAVIGVL
CHHHEECCCCCCCCCCCHHHCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHH
LDFPTIGVAKSRLTGDLVNESEITYVYLNGEKVGVKFGRYFYSPGNKVDLQDCIELGKRG
HCCCCCCHHHHHHCCCCCCCCCEEEEEECCCEEEEEEEEEEECCCCEECHHHHHHHCCCC
YPKVLKIADMLTKKIKKE
CHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVEKHLLEFLEKLQFLISKNVKISHYGIENVKKICGVDIAYKGNLGFSVGVSMDINSGDY
CCHHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCEEEECCCCEEEEEEEEECCCCC
NYKSYVGEVNFPYIPGFLFMREAPLMIKAIEGLDCHLLLVDGHGIAHPRKSGIAAVIGVL
CHHHEECCCCCCCCCCCHHHCCCCCEEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHH
LDFPTIGVAKSRLTGDLVNESEITYVYLNGEKVGVKFGRYFYSPGNKVDLQDCIELGKRG
HCCCCCCHHHHHHCCCCCCCCCEEEEEECCCEEEEEEEEEEECCCCEECHHHHHHHCCCC
YPKVLKIADMLTKKIKKE
CHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA