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The map label for this gene is entB-like2 [H]

Identifier: 15899200

GI number: 15899200

Start: 2226044

End: 2226661

Strand: Direct

Name: entB-like2 [H]

Synonym: SSO2455

Alternate gene names: 15899200

Gene position: 2226044-2226661 (Clockwise)

Preceding gene: 15899199

Following gene: 15899201

Centisome position: 74.39

GC content: 34.47

Gene sequence:

>618_bases
GTGCGTGCAGTAAATTTAATTATGAAAATTGAAGTACCATCTATTCCCGAGCATAAAGAGGTAATATTAGATCCTACTAA
TACAGCATTAATAATTGTAGACATGCAAAACGACTTTGTGAGGAAAAACGGTAAATTATCAGTTCCTACTGCAGAGGCTA
CTATACCATTTATAAAGAGATTAGTCGATAAGGCAAGAAGTTCCAATGCGTTGGTAATATATACACAAGATTGGCACATG
AAAGATGACCCAGAATTTAAAATATGGGGAGAGCACGCATTGGCTGGAACTTGGGGTGCAGAAATAATTGACGAATTAAC
TCCAGAAAAGAGCGATTTCATAGTTAAGAAGTATAGATATGATGCCTTCTTTGAATCCTCATTAGACTATATTCTTAGAG
TTAAGAATATCAAAAATACCATAATTACTGGGACTGTTGCAAATATTTGCGTATTACACACTGCTGGTAGTGCTGCTTTA
AGATGGTATAATGTAATTATGCCAAAGGATTCAATATCTGCAATAACGGAGTTTGATTATTATGCTACTTTAAGGCAAGT
AGACTTTTTATACAAGGGTATAATAACTACTGCAGATGGCATTAAATTTGAGAGGTAG

Upstream 100 bases:

>100_bases
GTCGACTTACAAGATTGCATTGAACTAGGTAAGAGGGGTTATCCTAAAGTTCTAAAAATAGCTGATATGCTCACTAAGAA
GATAAAAAAGGAATAAAACT

Downstream 100 bases:

>100_bases
GTAAAAAGTTGGAATATTATGCAATAGTACATAACGATTTTGATGGAACAGCATCTGCAGCCGTTTACGCTAGGGCAATT
AAATCATTACCTAAAAATGT

Product: isochorismatase, putative (entB-like2)

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MRAVNLIMKIEVPSIPEHKEVILDPTNTALIIVDMQNDFVRKNGKLSVPTAEATIPFIKRLVDKARSSNALVIYTQDWHM
KDDPEFKIWGEHALAGTWGAEIIDELTPEKSDFIVKKYRYDAFFESSLDYILRVKNIKNTIITGTVANICVLHTAGSAAL
RWYNVIMPKDSISAITEFDYYATLRQVDFLYKGIITTADGIKFER

Sequences:

>Translated_205_residues
MRAVNLIMKIEVPSIPEHKEVILDPTNTALIIVDMQNDFVRKNGKLSVPTAEATIPFIKRLVDKARSSNALVIYTQDWHM
KDDPEFKIWGEHALAGTWGAEIIDELTPEKSDFIVKKYRYDAFFESSLDYILRVKNIKNTIITGTVANICVLHTAGSAAL
RWYNVIMPKDSISAITEFDYYATLRQVDFLYKGIITTADGIKFER
>Mature_205_residues
MRAVNLIMKIEVPSIPEHKEVILDPTNTALIIVDMQNDFVRKNGKLSVPTAEATIPFIKRLVDKARSSNALVIYTQDWHM
KDDPEFKIWGEHALAGTWGAEIIDELTPEKSDFIVKKYRYDAFFESSLDYILRVKNIKNTIITGTVANICVLHTAGSAAL
RWYNVIMPKDSISAITEFDYYATLRQVDFLYKGIITTADGIKFER

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=202, Percent_Identity=27.7227722772277, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1786811, Length=192, Percent_Identity=28.125, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI87081970, Length=192, Percent_Identity=28.125, Blast_Score=65, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 23361; Mature: 23361

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAVNLIMKIEVPSIPEHKEVILDPTNTALIIVDMQNDFVRKNGKLSVPTAEATIPFIKR
CCCEEEEEEEECCCCCCCCEEEECCCCCEEEEEECCCCHHHCCCCEECCCCHHHHHHHHH
LVDKARSSNALVIYTQDWHMKDDPEFKIWGEHALAGTWGAEIIDELTPEKSDFIVKKYRY
HHHHHCCCCEEEEEEECCCCCCCCCEEEECCEEECCCHHHHHHHHHCCCCCCEEEEEHHH
DAFFESSLDYILRVKNIKNTIITGTVANICVLHTAGSAALRWYNVIMPKDSISAITEFDY
HHHHHCCHHEEEEEECCCCEEEECCHHEEEEEECCCCCEEEEEEEEECCCHHHHHHHHHH
YATLRQVDFLYKGIITTADGIKFER
HHHHHHHHHHHHHHEECCCCEEECC
>Mature Secondary Structure
MRAVNLIMKIEVPSIPEHKEVILDPTNTALIIVDMQNDFVRKNGKLSVPTAEATIPFIKR
CCCEEEEEEEECCCCCCCCEEEECCCCCEEEEEECCCCHHHCCCCEECCCCHHHHHHHHH
LVDKARSSNALVIYTQDWHMKDDPEFKIWGEHALAGTWGAEIIDELTPEKSDFIVKKYRY
HHHHHCCCCEEEEEEECCCCCCCCCEEEECCEEECCCHHHHHHHHHCCCCCCEEEEEHHH
DAFFESSLDYILRVKNIKNTIITGTVANICVLHTAGSAALRWYNVIMPKDSISAITEFDY
HHHHHCCHHEEEEEECCCCEEEECCHHEEEEEECCCCCEEEEEEEEECCCHHHHHHHHHH
YATLRQVDFLYKGIITTADGIKFER
HHHHHHHHHHHHHHEECCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA