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The map label for this gene is nudF [H]

Identifier: 15898948

GI number: 15898948

Start: 1992750

End: 1993250

Strand: Direct

Name: nudF [H]

Synonym: SSO2167

Alternate gene names: 15898948

Gene position: 1992750-1993250 (Clockwise)

Preceding gene: 15898947

Following gene: 15898951

Centisome position: 66.6

GC content: 33.53

Gene sequence:

>501_bases
ATGAAGATTTTTAGTGGTAAAAAATTTGAAGTGCACATAGATAAGGTGAAATTGCCTAACGGATATGAAAGGGAATTAGA
GTTCGTGAAGCATAGGGGTTCTGTCGTAATAATACCTAAGATAAACAATGAAATTATATTGATAAGGCAATTTAGGCCTG
TAATAGATAAATGGATATATGAGTTACCAGCAGGAACGATTGAGGAAGGAGAAGATCCATTAAATACTGCAAATAGAGAA
TTAATTGAGGAAATAGGATATGAAGCTGGGAAAATGAAAGAAATAATAAGTTTCTACGCTTCCCCTGGCATAACTACAGA
GTATATGAGATTATACTTGGCTGAAGATCTTAGATACGTCGGAGCTAAACCGGAACCATATGAAATCATAGAACCCATTA
GACTAAGTATAGAGGAGGCAATAAAAATGATAAGAGAAAGGAAAATAGAAGACGCAAAAACGATAATAGGAATATTTACA
TTAAAGGAGCTCTTAGAATAA

Upstream 100 bases:

>100_bases
TAGTATTCATTTTACTTGGAATTCTGTTATTCAAATTAGATTGGGAAGAAATTCTGAAGAGGGGAGAAAAATTCTTAAGA
GAAAAATTAAAAATTAATTT

Downstream 100 bases:

>100_bases
AAAGAGAAGCGTAGAAAAATCTAAAATTGTGTAATATTACAGAATGTTATGACAAGGTTCCCAATCGATGTGAGATCTCA
GTATGAATTGAACGTAAATA

Product: hypothetical protein

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 166; Mature: 166

Protein sequence:

>166_residues
MKIFSGKKFEVHIDKVKLPNGYERELEFVKHRGSVVIIPKINNEIILIRQFRPVIDKWIYELPAGTIEEGEDPLNTANRE
LIEEIGYEAGKMKEIISFYASPGITTEYMRLYLAEDLRYVGAKPEPYEIIEPIRLSIEEAIKMIRERKIEDAKTIIGIFT
LKELLE

Sequences:

>Translated_166_residues
MKIFSGKKFEVHIDKVKLPNGYERELEFVKHRGSVVIIPKINNEIILIRQFRPVIDKWIYELPAGTIEEGEDPLNTANRE
LIEEIGYEAGKMKEIISFYASPGITTEYMRLYLAEDLRYVGAKPEPYEIIEPIRLSIEEAIKMIRERKIEDAKTIIGIFT
LKELLE
>Mature_166_residues
MKIFSGKKFEVHIDKVKLPNGYERELEFVKHRGSVVIIPKINNEIILIRQFRPVIDKWIYELPAGTIEEGEDPLNTANRE
LIEEIGYEAGKMKEIISFYASPGITTEYMRLYLAEDLRYVGAKPEPYEIIEPIRLSIEEAIKMIRERKIEDAKTIIGIFT
LKELLE

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789800, Length=166, Percent_Identity=24.6987951807229, Blast_Score=63, Evalue=9e-12,
Organism=Escherichia coli, GI1789412, Length=160, Percent_Identity=31.875, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1788810, Length=175, Percent_Identity=29.7142857142857, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 19341; Mature: 19341

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIFSGKKFEVHIDKVKLPNGYERELEFVKHRGSVVIIPKINNEIILIRQFRPVIDKWIY
CCCCCCCEEEEEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCEEEEEHHHHHHHHHHH
ELPAGTIEEGEDPLNTANRELIEEIGYEAGKMKEIISFYASPGITTEYMRLYLAEDLRYV
HCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
GAKPEPYEIIEPIRLSIEEAIKMIRERKIEDAKTIIGIFTLKELLE
CCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MKIFSGKKFEVHIDKVKLPNGYERELEFVKHRGSVVIIPKINNEIILIRQFRPVIDKWIY
CCCCCCCEEEEEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCCEEEEEHHHHHHHHHHH
ELPAGTIEEGEDPLNTANRELIEEIGYEAGKMKEIISFYASPGITTEYMRLYLAEDLRYV
HCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHC
GAKPEPYEIIEPIRLSIEEAIKMIRERKIEDAKTIIGIFTLKELLE
CCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10542272 [H]