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The map label for this gene is pdhD-3 [H]

Identifier: 15898383

GI number: 15898383

Start: 1410329

End: 1411699

Strand: Reverse

Name: pdhD-3 [H]

Synonym: SSO1565

Alternate gene names: 15898383

Gene position: 1411699-1410329 (Counterclockwise)

Preceding gene: 15898389

Following gene: 15898382

Centisome position: 47.18

GC content: 40.55

Gene sequence:

>1371_bases
ATGAAATATGATGTAGTTGTTATAGGAGCTGGAGGAGCAGGATATCATGGGGCCTTCAGGCTTGCAAAGGCAAAATACAA
CGTATTGATGGCTGATCCCAAAGGTGAATTAGGAGGGAATTGCTTGTATAGTGGATGTGTACCATCTAAGACAGTTAGAG
AAGTGATACAAACTGCCTGGAGACTTACAAACATAGCTAACGTAAAGATCCCCCTAGATTTTTCAACCGTTCAAGATCGC
AAAGATTACGTTCAAGAGTTGAGATTCAAGCAGCATAAGAGGAACATGTCCCAATACGAAACTCTAACCTTCTATAAGGG
ATACGTTAAGATTAAGGACCCAACCCACGTGATAGTTAAAACGGATGAGGGAAAGGAAATTGAGGCTGAGACTAGGTATA
TGATAATAGCCAGTGGTGCTGAAACCGCTAAACTGAGGTTACCTGGAGTTGAATACTGTTTAACAAGCGACGATATATTT
GGGTATAAGACGTCATTTAGAAAGCTACCTCAGGACATGGTGATCATAGGAGCTGGGTATATAGGACTCGAAATTGCGTC
AATCTTTAGATTAATGGGTGTACAAACTCACATTATAGAAATGCTAGATAGAGCCTTAATAACGCTTGAGGATCAAGATA
TCGTCAATACTTTACTATCAATACTTAAACTTAACATAAAGTTCAACTCTCCCGTAACTGAAGTTAAGAAAATTAAGGAT
GATGAGTACGAAGTAATTTATTCGACCAAAGATGGTTCCAAAAAGAGCATTTTCACAAACTCTGTAGTGTTAGCTGCTGG
GAGAAGACCGGTTATCCCAGAAGGGGCAAGGGAAATAGGACTTTCAATAAGCAAGACTGGGATAGTTGTCGACGAGACAA
TGAAGACTAACATTCCAAATGTTTTCGCGACAGGAGACGCAAATGGTTTAGCCCCATATTATCACGCTGCAGTGAGAATG
AGTATAGCTGCAGCAAACAACATAATGGCTAATGGAATGCCAGTGGATTATGTGGACGTTAAGAGCATACCAGTAACGAT
ATACACGATTCCTTCACTATCCTATGTTGGAATATTACCTAGCAAGGCTAGAAAAATGGGCATTGAGATAGTTGAGGCGG
AATACAACATGGAAGAGGATGTGTCAGCGCAAATCTACGGACAGAAAGAGGGCGTACTTAAGCTAATATTTGAAAGGGGA
AGTATGAGGTTAATCGGGGCTTGGATGATTGGAGTCCACTCTCAATACTTAATTAACGAATTGGGATTGGCAGTAGCTTA
CGGACTAAACGCTAAACAACTTGCTAGTTTCGCTGAGCAACATCCATCTACGAACGAGATTATATCTTATACGGCTAGGA
AGGTCATATAA

Upstream 100 bases:

>100_bases
CGTGAAGAATAAGTATTTAATCTCATCATAGTTTGAATGTTATTAGATACTGTTAAACAAGAAAAAATAATAAATACTTT
GATAACTAATAGTTATAATT

Downstream 100 bases:

>100_bases
TTACCTAATTTTTTCCTTTTAATCTTTTAACGTTAATAAGCCCCTATTTAATAAAAACGACTACTACCTTTAGCAAATCA
TTCCGCCGTGGATAACAGTT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MKYDVVVIGAGGAGYHGAFRLAKAKYNVLMADPKGELGGNCLYSGCVPSKTVREVIQTAWRLTNIANVKIPLDFSTVQDR
KDYVQELRFKQHKRNMSQYETLTFYKGYVKIKDPTHVIVKTDEGKEIEAETRYMIIASGAETAKLRLPGVEYCLTSDDIF
GYKTSFRKLPQDMVIIGAGYIGLEIASIFRLMGVQTHIIEMLDRALITLEDQDIVNTLLSILKLNIKFNSPVTEVKKIKD
DEYEVIYSTKDGSKKSIFTNSVVLAAGRRPVIPEGAREIGLSISKTGIVVDETMKTNIPNVFATGDANGLAPYYHAAVRM
SIAAANNIMANGMPVDYVDVKSIPVTIYTIPSLSYVGILPSKARKMGIEIVEAEYNMEEDVSAQIYGQKEGVLKLIFERG
SMRLIGAWMIGVHSQYLINELGLAVAYGLNAKQLASFAEQHPSTNEIISYTARKVI

Sequences:

>Translated_456_residues
MKYDVVVIGAGGAGYHGAFRLAKAKYNVLMADPKGELGGNCLYSGCVPSKTVREVIQTAWRLTNIANVKIPLDFSTVQDR
KDYVQELRFKQHKRNMSQYETLTFYKGYVKIKDPTHVIVKTDEGKEIEAETRYMIIASGAETAKLRLPGVEYCLTSDDIF
GYKTSFRKLPQDMVIIGAGYIGLEIASIFRLMGVQTHIIEMLDRALITLEDQDIVNTLLSILKLNIKFNSPVTEVKKIKD
DEYEVIYSTKDGSKKSIFTNSVVLAAGRRPVIPEGAREIGLSISKTGIVVDETMKTNIPNVFATGDANGLAPYYHAAVRM
SIAAANNIMANGMPVDYVDVKSIPVTIYTIPSLSYVGILPSKARKMGIEIVEAEYNMEEDVSAQIYGQKEGVLKLIFERG
SMRLIGAWMIGVHSQYLINELGLAVAYGLNAKQLASFAEQHPSTNEIISYTARKVI
>Mature_456_residues
MKYDVVVIGAGGAGYHGAFRLAKAKYNVLMADPKGELGGNCLYSGCVPSKTVREVIQTAWRLTNIANVKIPLDFSTVQDR
KDYVQELRFKQHKRNMSQYETLTFYKGYVKIKDPTHVIVKTDEGKEIEAETRYMIIASGAETAKLRLPGVEYCLTSDDIF
GYKTSFRKLPQDMVIIGAGYIGLEIASIFRLMGVQTHIIEMLDRALITLEDQDIVNTLLSILKLNIKFNSPVTEVKKIKD
DEYEVIYSTKDGSKKSIFTNSVVLAAGRRPVIPEGAREIGLSISKTGIVVDETMKTNIPNVFATGDANGLAPYYHAAVRM
SIAAANNIMANGMPVDYVDVKSIPVTIYTIPSLSYVGILPSKARKMGIEIVEAEYNMEEDVSAQIYGQKEGVLKLIFERG
SMRLIGAWMIGVHSQYLINELGLAVAYGLNAKQLASFAEQHPSTNEIISYTARKVI

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=466, Percent_Identity=27.2532188841202, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI50301238, Length=475, Percent_Identity=24.6315789473684, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI33519430, Length=440, Percent_Identity=24.7727272727273, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI33519428, Length=440, Percent_Identity=24.7727272727273, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI33519426, Length=440, Percent_Identity=24.7727272727273, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI148277065, Length=446, Percent_Identity=24.6636771300448, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI148277071, Length=446, Percent_Identity=24.6636771300448, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI22035672, Length=446, Percent_Identity=23.0941704035874, Blast_Score=85, Evalue=1e-16,
Organism=Homo sapiens, GI291045266, Length=453, Percent_Identity=22.2958057395143, Blast_Score=79, Evalue=9e-15,
Organism=Homo sapiens, GI291045268, Length=302, Percent_Identity=24.1721854304636, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1786307, Length=472, Percent_Identity=28.8135593220339, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI87081717, Length=454, Percent_Identity=26.8722466960352, Blast_Score=126, Evalue=3e-30,
Organism=Escherichia coli, GI87082354, Length=446, Percent_Identity=26.6816143497758, Blast_Score=106, Evalue=3e-24,
Organism=Escherichia coli, GI1789915, Length=426, Percent_Identity=23.943661971831, Blast_Score=105, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI32565766, Length=463, Percent_Identity=25.4859611231102, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17557007, Length=492, Percent_Identity=24.7967479674797, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI71983429, Length=433, Percent_Identity=24.9422632794457, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71983419, Length=433, Percent_Identity=24.9422632794457, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71982272, Length=366, Percent_Identity=25.6830601092896, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321091, Length=473, Percent_Identity=27.2727272727273, Blast_Score=154, Evalue=3e-38,
Organism=Saccharomyces cerevisiae, GI6325166, Length=465, Percent_Identity=24.5161290322581, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6325240, Length=478, Percent_Identity=25.5230125523013, Blast_Score=108, Evalue=2e-24,
Organism=Drosophila melanogaster, GI21358499, Length=472, Percent_Identity=25.8474576271186, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI17737741, Length=486, Percent_Identity=25.3086419753086, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24640553, Length=488, Percent_Identity=25.4098360655738, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24640549, Length=488, Percent_Identity=25.4098360655738, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24640551, Length=488, Percent_Identity=25.4098360655738, Blast_Score=94, Evalue=2e-19,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50498; Mature: 50498

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYDVVVIGAGGAGYHGAFRLAKAKYNVLMADPKGELGGNCLYSGCVPSKTVREVIQTAW
CCEEEEEEECCCCCCCHHEEEEEEEEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHH
RLTNIANVKIPLDFSTVQDRKDYVQELRFKQHKRNMSQYETLTFYKGYVKIKDPTHVIVK
HHHCEEEEEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEEEEEEEEECCCCEEEEE
TDEGKEIEAETRYMIIASGAETAKLRLPGVEYCLTSDDIFGYKTSFRKLPQDMVIIGAGY
CCCCCEEECCCEEEEEECCCCEEEEECCCCEEEEECCCCCCCHHHHHHCCCCEEEEECCH
IGLEIASIFRLMGVQTHIIEMLDRALITLEDQDIVNTLLSILKLNIKFNSPVTEVKKIKD
HHHHHHHHHHHHCHHHHHHHHHHHHHEEECCHHHHHHHHHHHEEEEEECCCHHHHHHCCC
DEYEVIYSTKDGSKKSIFTNSVVLAAGRRPVIPEGAREIGLSISKTGIVVDETMKTNIPN
CCEEEEEECCCCCCCEEEECCEEEECCCCCCCCCCHHHHCCEEECCCEEEECHHHCCCCE
VFATGDANGLAPYYHAAVRMSIAAANNIMANGMPVDYVDVKSIPVTIYTIPSLSYVGILP
EEEECCCCCCCHHHHHHHHEEEHHHCCEEECCCCCCEEECCCCCEEEEEECCCEEEECCC
SKARKMGIEIVEAEYNMEEDVSAQIYGQKEGVLKLIFERGSMRLIGAWMIGVHSQYLINE
HHHHHCCCEEEEECCCCCCCCCEEEECCCCCCEEEEECCCCEEEEEEEEHHCCHHHHHHH
LGLAVAYGLNAKQLASFAEQHPSTNEIISYTARKVI
HHHHEEECCCHHHHHHHHHHCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKYDVVVIGAGGAGYHGAFRLAKAKYNVLMADPKGELGGNCLYSGCVPSKTVREVIQTAW
CCEEEEEEECCCCCCCHHEEEEEEEEEEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHH
RLTNIANVKIPLDFSTVQDRKDYVQELRFKQHKRNMSQYETLTFYKGYVKIKDPTHVIVK
HHHCEEEEEEEECHHHHHHHHHHHHHHHHHHHHCCHHHHEEEEEEEEEEEECCCCEEEEE
TDEGKEIEAETRYMIIASGAETAKLRLPGVEYCLTSDDIFGYKTSFRKLPQDMVIIGAGY
CCCCCEEECCCEEEEEECCCCEEEEECCCCEEEEECCCCCCCHHHHHHCCCCEEEEECCH
IGLEIASIFRLMGVQTHIIEMLDRALITLEDQDIVNTLLSILKLNIKFNSPVTEVKKIKD
HHHHHHHHHHHHCHHHHHHHHHHHHHEEECCHHHHHHHHHHHEEEEEECCCHHHHHHCCC
DEYEVIYSTKDGSKKSIFTNSVVLAAGRRPVIPEGAREIGLSISKTGIVVDETMKTNIPN
CCEEEEEECCCCCCCEEEECCEEEECCCCCCCCCCHHHHCCEEECCCEEEECHHHCCCCE
VFATGDANGLAPYYHAAVRMSIAAANNIMANGMPVDYVDVKSIPVTIYTIPSLSYVGILP
EEEECCCCCCCHHHHHHHHEEEHHHCCEEECCCCCCEEECCCCCEEEEEECCCEEEECCC
SKARKMGIEIVEAEYNMEEDVSAQIYGQKEGVLKLIFERGSMRLIGAWMIGVHSQYLINE
HHHHHCCCEEEEECCCCCCCCCEEEECCCCCCEEEEECCCCEEEEEEEEHHCCHHHHHHH
LGLAVAYGLNAKQLASFAEQHPSTNEIISYTARKVI
HHHHEEECCCHHHHHHHHHHCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]