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The map label for this gene is pdhB-2 [H]

Identifier: 15898354

GI number: 15898354

Start: 1378387

End: 1379361

Strand: Direct

Name: pdhB-2 [H]

Synonym: SSO1526

Alternate gene names: 15898354

Gene position: 1378387-1379361 (Clockwise)

Preceding gene: 15898353

Following gene: 15898355

Centisome position: 46.07

GC content: 40.1

Gene sequence:

>975_bases
ATGAGGCAGATAACGTTTACTGAAGCAATAAATGAGGCCTTAAGGCAAGAAATGGAAAGAGATCCCTCAGTCATATTAAT
AGGGGAAGATATAGGTGTTTATGGCGGAGCTTTTGGAGTAACTAAGGGTTTAATTGAGAAATTTGGCTCCGATAGAGTTA
TTGATACTCCAATATCTGAGGCTGGGTTCATAGGTGCAGCAGTAGGTGCTGCATTAGCTGGCTTAAGACCAGTAGTGGAG
TTAATGTTTGTAGATTTCTTTGGAGTTGCAATGGATCAGATCTATAATCAAATGGCTAAATTAAGGTATATGTCTGGAGG
GCAATTGAAAGTACCCCTTACTTTAAGAGCACCAATAGGTGCGGGAATAAGTGCTGCAGCTCAACATTCGCAAACCCTTT
ACTCAATTTTCGCTCACGTACCTGGTTTAAAGGTAGTTGTACCCTCTACTCCTCACGATGCTAAAGGTTTGTTAATCTCA
TCGATACGTGATGATAACCCAGTAGTATTCTTAGAACATAAGGTGTTATATGGGATAAAAGGTGAAGTACCGGAAGAAGA
GTACACTATACCTCTAGGTAAGGCAGAGATAAGAAGAGAAGGAGATGACGTAACAGTAATTGGAATAGCCAGAACAGTAT
GGCATAGTTTAGAGGCTGCGGAACAACTGTCTAAGGAAAGTATAAGTGTGGAGGTTATTGATGTGAGATCAATAGTTCCT
TTCGATAAGGAGACCGTTATTAAATCGGTTAAGAAAACGGGGAGGGTTGTAATTGTGGATGAGGATTATGACAGATGTGG
CTTCGCCTCATGGGTTTCTTCCATAATCGCAGACGAAGCCTTTGAATACTTGGATGCGCCAATAAAGAGAATAACTACTC
CTAACGTCCCTATACCCTTCAGTCCTCCCTTAGAGCAATATATATTACCAGATTCTAAAAAGATTGTTAATACAGTTAAA
TCAATATTGGGGTAA

Upstream 100 bases:

>100_bases
TAGAGCAAGCATTGAAATTTGCTGAGGAAAGTCCATATCCAGAAGTAGAAGAAGCTCTCACTGACGTTTTTACAGACAAC
TCGTATTAGGGTGCATGTAA

Downstream 100 bases:

>100_bases
ATTTTGATTGCCGGCTGTAGGAATTATTGTTAACCCTGAATCTGGAAGGGATATTAGAAGGCTAGTTGCAAAAGCTACCT
TTGTCTCTAACTACGCTAAG

Product: pyruvate dehydrogenase beta subunit (lipoamide)

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-beta; Ao:DCPIP OR; TPP-dependent acetoin dehydrogenase E1 subunit beta [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MRQITFTEAINEALRQEMERDPSVILIGEDIGVYGGAFGVTKGLIEKFGSDRVIDTPISEAGFIGAAVGAALAGLRPVVE
LMFVDFFGVAMDQIYNQMAKLRYMSGGQLKVPLTLRAPIGAGISAAAQHSQTLYSIFAHVPGLKVVVPSTPHDAKGLLIS
SIRDDNPVVFLEHKVLYGIKGEVPEEEYTIPLGKAEIRREGDDVTVIGIARTVWHSLEAAEQLSKESISVEVIDVRSIVP
FDKETVIKSVKKTGRVVIVDEDYDRCGFASWVSSIIADEAFEYLDAPIKRITTPNVPIPFSPPLEQYILPDSKKIVNTVK
SILG

Sequences:

>Translated_324_residues
MRQITFTEAINEALRQEMERDPSVILIGEDIGVYGGAFGVTKGLIEKFGSDRVIDTPISEAGFIGAAVGAALAGLRPVVE
LMFVDFFGVAMDQIYNQMAKLRYMSGGQLKVPLTLRAPIGAGISAAAQHSQTLYSIFAHVPGLKVVVPSTPHDAKGLLIS
SIRDDNPVVFLEHKVLYGIKGEVPEEEYTIPLGKAEIRREGDDVTVIGIARTVWHSLEAAEQLSKESISVEVIDVRSIVP
FDKETVIKSVKKTGRVVIVDEDYDRCGFASWVSSIIADEAFEYLDAPIKRITTPNVPIPFSPPLEQYILPDSKKIVNTVK
SILG
>Mature_324_residues
MRQITFTEAINEALRQEMERDPSVILIGEDIGVYGGAFGVTKGLIEKFGSDRVIDTPISEAGFIGAAVGAALAGLRPVVE
LMFVDFFGVAMDQIYNQMAKLRYMSGGQLKVPLTLRAPIGAGISAAAQHSQTLYSIFAHVPGLKVVVPSTPHDAKGLLIS
SIRDDNPVVFLEHKVLYGIKGEVPEEEYTIPLGKAEIRREGDDVTVIGIARTVWHSLEAAEQLSKESISVEVIDVRSIVP
FDKETVIKSVKKTGRVVIVDEDYDRCGFASWVSSIIADEAFEYLDAPIKRITTPNVPIPFSPPLEQYILPDSKKIVNTVK
SILG

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde [H]

COG id: COG0022

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI156564403, Length=326, Percent_Identity=46.6257668711656, Blast_Score=298, Evalue=4e-81,
Organism=Homo sapiens, GI291084858, Length=327, Percent_Identity=44.3425076452599, Blast_Score=275, Evalue=3e-74,
Organism=Homo sapiens, GI4557353, Length=326, Percent_Identity=40.1840490797546, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI34101272, Length=326, Percent_Identity=40.1840490797546, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI225637463, Length=240, Percent_Identity=27.9166666666667, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI133778974, Length=243, Percent_Identity=31.6872427983539, Blast_Score=76, Evalue=5e-14,
Organism=Homo sapiens, GI225637459, Length=240, Percent_Identity=27.9166666666667, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI225637461, Length=240, Percent_Identity=27.9166666666667, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI205277463, Length=251, Percent_Identity=27.4900398406374, Blast_Score=72, Evalue=5e-13,
Organism=Homo sapiens, GI4507521, Length=251, Percent_Identity=27.4900398406374, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI17538422, Length=326, Percent_Identity=46.319018404908, Blast_Score=300, Evalue=9e-82,
Organism=Caenorhabditis elegans, GI17506935, Length=322, Percent_Identity=41.6149068322981, Blast_Score=226, Evalue=1e-59,
Organism=Caenorhabditis elegans, GI17539652, Length=267, Percent_Identity=28.4644194756554, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6319698, Length=328, Percent_Identity=47.5609756097561, Blast_Score=308, Evalue=9e-85,
Organism=Drosophila melanogaster, GI21358145, Length=328, Percent_Identity=43.5975609756098, Blast_Score=283, Evalue=8e-77,
Organism=Drosophila melanogaster, GI24650940, Length=328, Percent_Identity=43.5975609756098, Blast_Score=283, Evalue=8e-77,
Organism=Drosophila melanogaster, GI160714832, Length=326, Percent_Identity=41.7177914110429, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI160714828, Length=326, Percent_Identity=41.7177914110429, Blast_Score=239, Evalue=2e-63,
Organism=Drosophila melanogaster, GI24650943, Length=92, Percent_Identity=51.0869565217391, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24650945, Length=92, Percent_Identity=51.0869565217391, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24645119, Length=288, Percent_Identity=27.7777777777778, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI45551847, Length=295, Percent_Identity=27.1186440677966, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI45550715, Length=295, Percent_Identity=27.1186440677966, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009014
- InterPro:   IPR015941
- InterPro:   IPR005475
- InterPro:   IPR005476 [H]

Pfam domain/function: PF02779 Transket_pyr; PF02780 Transketolase_C [H]

EC number: 1.2.4.1

Molecular weight: Translated: 35324; Mature: 35324

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQITFTEAINEALRQEMERDPSVILIGEDIGVYGGAFGVTKGLIEKFGSDRVIDTPISE
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEECCCCC
AGFIGAAVGAALAGLRPVVELMFVDFFGVAMDQIYNQMAKLRYMSGGQLKVPLTLRAPIG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
AGISAAAQHSQTLYSIFAHVPGLKVVVPSTPHDAKGLLISSIRDDNPVVFLEHKVLYGIK
CCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEECCCCCCCEEEEECEEEECCC
GEVPEEEYTIPLGKAEIRREGDDVTVIGIARTVWHSLEAAEQLSKESISVEVIDVRSIVP
CCCCCCCEECCCCHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCEEEEEEEHHCCC
FDKETVIKSVKKTGRVVIVDEDYDRCGFASWVSSIIADEAFEYLDAPIKRITTPNVPIPF
CCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
SPPLEQYILPDSKKIVNTVKSILG
CCCHHHHCCCCCHHHHHHHHHHHC
>Mature Secondary Structure
MRQITFTEAINEALRQEMERDPSVILIGEDIGVYGGAFGVTKGLIEKFGSDRVIDTPISE
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHCCCCEEECCCCC
AGFIGAAVGAALAGLRPVVELMFVDFFGVAMDQIYNQMAKLRYMSGGQLKVPLTLRAPIG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCC
AGISAAAQHSQTLYSIFAHVPGLKVVVPSTPHDAKGLLISSIRDDNPVVFLEHKVLYGIK
CCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEECCCCCCCEEEEECEEEECCC
GEVPEEEYTIPLGKAEIRREGDDVTVIGIARTVWHSLEAAEQLSKESISVEVIDVRSIVP
CCCCCCCEECCCCHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCEEEEEEEHHCCC
FDKETVIKSVKKTGRVVIVDEDYDRCGFASWVSSIIADEAFEYLDAPIKRITTPNVPIPF
CCHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
SPPLEQYILPDSKKIVNTVKSILG
CCCHHHHCCCCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10368162; 8969503; 9384377 [H]