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The map label for this gene is pdhA-2 [H]

Identifier: 15898353

GI number: 15898353

Start: 1377377

End: 1378375

Strand: Direct

Name: pdhA-2 [H]

Synonym: SSO1525

Alternate gene names: 15898353

Gene position: 1377377-1378375 (Clockwise)

Preceding gene: 15898352

Following gene: 15898354

Centisome position: 46.03

GC content: 39.34

Gene sequence:

>999_bases
ATGTTTCAAGATATCCCTAAAAGTAAATTATTAGACATGTATAAAAAGATGCTATTAATTAGATACCATGAGCTTACTGC
TAAAGAGCTCTTCGCATCTGGTAAGATTCCGGGATTTGTGCATCTCTATGTAGGTGAAGAGGCTGTAGCTGTAGGGGTTA
TGAGTACGCTAAGAGATGATGACTATATAACTAGTACGCATAGAGGACATGGGCATTGTATAGCTAAGGGTTTAGACGTA
AAGAGGATGTTAGCAGAGATAATGGGTAAGAAAACTGGAGTGTGTAAAGGAAAAGGTGGATCGATGCACATTTTTGATTA
TAGTAAAGGTATGTTAGGCGCAAACGGTATAGTTGGTGGAGGAGCTCCTCATGCTGTAGGCGCGGCATTAGCGTTTAAAC
TTAAAGGTTTGGATCGTGTAGCTGTGGCATTTATTGGAGATGGGGCTATGAATCAAGGTGTGGTTTTGGAGTCTTTAAAT
CTCTCTGCTATATGGAAACTTCCAGTAATATTTGTAGTAGAAGATAACATGTATGCGATGTCAACTAGAAGTTTAGTACC
TGGTAAATTACAGCCTAGGCATTCAGCTGCTAAAAGTTATGTTGAGAGGGCCTTAGGTTTTGGAATACCAGCTGTAGAAG
TGGATGGGATGGACGTACTAGCAGTTTATGAGGTGGCTAAAGAAGCTGTTGATAGGGCTAGAAGAGGAGGAGGCCCATCC
TTATTACATTGTAAGACTTACAGATTCTTTGGACACTTTGAGGGAGATCCTTTAGTTTATAGAGATAAAGAGGAGGAAGA
GATGTGGAGAAAGAGGGATCCTATTACTCTATTTAGAGATAAACTTGTTTCAAATAATATTATAAATTCAGAGGAATTGG
ATAAGATAGACAGAGAGGCTAAAGCGGAAATAGAGCAAGCATTGAAATTTGCTGAGGAAAGTCCATATCCAGAAGTAGAA
GAAGCTCTCACTGACGTTTTTACAGACAACTCGTATTAG

Upstream 100 bases:

>100_bases
CCGCAAATCTAAAATTGCTTGCAACAACGACGTTTTACCCTCTCATAGCTATTTTGTTAACATTTAAATACTTCAATTTT
AATATACTTTTATTGAGATA

Downstream 100 bases:

>100_bases
GGTGCATGTAAATGAGGCAGATAACGTTTACTGAAGCAATAAATGAGGCCTTAAGGCAAGAAATGGAAAGAGATCCCTCA
GTCATATTAATAGGGGAAGA

Product: pyruvate dehydrogenase alpha subunit (lipoamide)

Products: [dihydrolipoyllysine-residue acetyltransferase] ; $S-acetyldihydrolipoyllysine; CO2

Alternate protein names: Acetoin:DCPIP oxidoreductase-alpha; Ao:DCPIP OR [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MFQDIPKSKLLDMYKKMLLIRYHELTAKELFASGKIPGFVHLYVGEEAVAVGVMSTLRDDDYITSTHRGHGHCIAKGLDV
KRMLAEIMGKKTGVCKGKGGSMHIFDYSKGMLGANGIVGGGAPHAVGAALAFKLKGLDRVAVAFIGDGAMNQGVVLESLN
LSAIWKLPVIFVVEDNMYAMSTRSLVPGKLQPRHSAAKSYVERALGFGIPAVEVDGMDVLAVYEVAKEAVDRARRGGGPS
LLHCKTYRFFGHFEGDPLVYRDKEEEEMWRKRDPITLFRDKLVSNNIINSEELDKIDREAKAEIEQALKFAEESPYPEVE
EALTDVFTDNSY

Sequences:

>Translated_332_residues
MFQDIPKSKLLDMYKKMLLIRYHELTAKELFASGKIPGFVHLYVGEEAVAVGVMSTLRDDDYITSTHRGHGHCIAKGLDV
KRMLAEIMGKKTGVCKGKGGSMHIFDYSKGMLGANGIVGGGAPHAVGAALAFKLKGLDRVAVAFIGDGAMNQGVVLESLN
LSAIWKLPVIFVVEDNMYAMSTRSLVPGKLQPRHSAAKSYVERALGFGIPAVEVDGMDVLAVYEVAKEAVDRARRGGGPS
LLHCKTYRFFGHFEGDPLVYRDKEEEEMWRKRDPITLFRDKLVSNNIINSEELDKIDREAKAEIEQALKFAEESPYPEVE
EALTDVFTDNSY
>Mature_332_residues
MFQDIPKSKLLDMYKKMLLIRYHELTAKELFASGKIPGFVHLYVGEEAVAVGVMSTLRDDDYITSTHRGHGHCIAKGLDV
KRMLAEIMGKKTGVCKGKGGSMHIFDYSKGMLGANGIVGGGAPHAVGAALAFKLKGLDRVAVAFIGDGAMNQGVVLESLN
LSAIWKLPVIFVVEDNMYAMSTRSLVPGKLQPRHSAAKSYVERALGFGIPAVEVDGMDVLAVYEVAKEAVDRARRGGGPS
LLHCKTYRFFGHFEGDPLVYRDKEEEEMWRKRDPITLFRDKLVSNNIINSEELDKIDREAKAEIEQALKFAEESPYPEVE
EALTDVFTDNSY

Specific function: Catalyzes the 2,6-dichlorophenolindophenol-dependent cleavage of acetoin into acetate and acetaldehyde, in vitro. The alpha subunit is probably the catalytic subunit of the enzyme [H]

COG id: COG1071

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase (E1) component, eukaryotic type, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI4885543, Length=329, Percent_Identity=41.0334346504559, Blast_Score=261, Evalue=8e-70,
Organism=Homo sapiens, GI4505685, Length=322, Percent_Identity=41.9254658385093, Blast_Score=230, Evalue=1e-60,
Organism=Homo sapiens, GI291084742, Length=322, Percent_Identity=41.9254658385093, Blast_Score=230, Evalue=2e-60,
Organism=Homo sapiens, GI291084744, Length=329, Percent_Identity=41.0334346504559, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI291084757, Length=322, Percent_Identity=37.5776397515528, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI258645172, Length=327, Percent_Identity=29.3577981651376, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI11386135, Length=327, Percent_Identity=29.3577981651376, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17536047, Length=319, Percent_Identity=39.8119122257053, Blast_Score=231, Evalue=5e-61,
Organism=Caenorhabditis elegans, GI32564172, Length=319, Percent_Identity=39.8119122257053, Blast_Score=231, Evalue=6e-61,
Organism=Caenorhabditis elegans, GI86563355, Length=320, Percent_Identity=28.4375, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI86563357, Length=320, Percent_Identity=28.4375, Blast_Score=120, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6321026, Length=328, Percent_Identity=39.9390243902439, Blast_Score=237, Evalue=2e-63,
Organism=Drosophila melanogaster, GI24639740, Length=328, Percent_Identity=39.9390243902439, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24639744, Length=328, Percent_Identity=39.9390243902439, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI28571106, Length=328, Percent_Identity=39.9390243902439, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI24639746, Length=316, Percent_Identity=40.5063291139241, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24639748, Length=329, Percent_Identity=38.6018237082067, Blast_Score=215, Evalue=3e-56,
Organism=Drosophila melanogaster, GI21355903, Length=323, Percent_Identity=24.4582043343653, Blast_Score=99, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001017 [H]

Pfam domain/function: PF00676 E1_dh [H]

EC number: 1.2.4.1

Molecular weight: Translated: 36692; Mature: 36692

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFQDIPKSKLLDMYKKMLLIRYHELTAKELFASGKIPGFVHLYVGEEAVAVGVMSTLRDD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHCCC
DYITSTHRGHGHCIAKGLDVKRMLAEIMGKKTGVCKGKGGSMHIFDYSKGMLGANGIVGG
CCEECCCCCCCEEEECCCCHHHHHHHHHCCCCCCEECCCCEEEEEECCCCCCCCCCCCCC
GAPHAVGAALAFKLKGLDRVAVAFIGDGAMNQGVVLESLNLSAIWKLPVIFVVEDNMYAM
CCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEECCCCEEEEECCEEEEEECCEEEE
STRSLVPGKLQPRHSAAKSYVERALGFGIPAVEVDGMDVLAVYEVAKEAVDRARRGGGPS
ECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCC
LLHCKTYRFFGHFEGDPLVYRDKEEEEMWRKRDPITLFRDKLVSNNIINSEELDKIDREA
EEEEEEEEEEEEECCCEEEECCCCHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
KAEIEQALKFAEESPYPEVEEALTDVFTDNSY
HHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC
>Mature Secondary Structure
MFQDIPKSKLLDMYKKMLLIRYHELTAKELFASGKIPGFVHLYVGEEAVAVGVMSTLRDD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHCCC
DYITSTHRGHGHCIAKGLDVKRMLAEIMGKKTGVCKGKGGSMHIFDYSKGMLGANGIVGG
CCEECCCCCCCEEEECCCCHHHHHHHHHCCCCCCEECCCCEEEEEECCCCCCCCCCCCCC
GAPHAVGAALAFKLKGLDRVAVAFIGDGAMNQGVVLESLNLSAIWKLPVIFVVEDNMYAM
CCCHHHHHHHHHHHCCCCEEEEEEECCCCCCCCEEEECCCCEEEEECCEEEEEECCEEEE
STRSLVPGKLQPRHSAAKSYVERALGFGIPAVEVDGMDVLAVYEVAKEAVDRARRGGGPS
ECCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCC
LLHCKTYRFFGHFEGDPLVYRDKEEEEMWRKRDPITLFRDKLVSNNIINSEELDKIDREA
EEEEEEEEEEEEECCCEEEECCCCHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHH
KAEIEQALKFAEESPYPEVEEALTDVFTDNSY
HHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: pyruvate; [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine

Specific reaction: pyruvate + [dihydrolipoyllysine-residue acetyltransferase] lipoyllysine = [dihydrolipoyllysine-residue acetyltransferase] S- $acetyldihydrolipoyllysine + CO2

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2061286 [H]