Click here to switch to the map view.

The map label for this gene is acsA-2 [H]

Identifier: 15898183

GI number: 15898183

Start: 1178229

End: 1179092

Strand: Reverse

Name: acsA-2 [H]

Synonym: SSO1342

Alternate gene names: 15898183

Gene position: 1179092-1178229 (Counterclockwise)

Preceding gene: 15898186

Following gene: 15898181

Centisome position: 39.4

GC content: 37.85

Gene sequence:

>864_bases
ATGCTCGAGGTAGATTTGAGGGAAATTAGATTTCATAATTATGATGAACTTGTGAGAACATTCAAATGGAATATTCCTTC
TCGCTTTAATATAGGAGAATCGATATTAGATAGAAAGGTTAAGGAAGGTGTAGGAGATAACATCGCAATATACTATGAAG
ACGAGGAAGGTGATCATTTCGTTTATACTTTCGCCCAATTAAAGAGTCTTAGCGATTCTTTAATAACAATTTTAAGAGAA
ATAGGAGTAAAAAGAGGGGATGTGGTTGGTATTTACTTACAGCCCAGGGTTGAAACTGTAATAAGTATTTTATCACTATA
TAGGCTTGGAGCAATTAGCTTATCGATTTCACCATTAATGGGAGTAGAGGCTGTTGAATATAGAATAAGACAAAGTGATG
CCAAGGCTATAATAATTGAAGGTTCAAGAAAAGAGGTTAGAGAAAAATTAAGGAACGTAACTAAGATTATAGTTGCTGAT
GAAGCTAGAGATGAAAGTGAAATTAATTTCGATGAGGTTAAAAAGACCTCTGGAGTACATTATGCGGTAGATACTAAGAG
TGATGATCCAGCCCAACTCTTTTATACCTCTGGTAGTACTGGAGCACCAAAGGGAGTCCTTCATGCTCATAGATTTCTGT
TGGGACATATTCCTGCCTATCAGCTCTATTTCGAAATGGCACCAAGAGAAGAGGATGTATTTTATACACCCGCGGACTGG
GGCTGGATTGGGGCTATAGGCGATGTAATACTACCTAGCCTCTATTTCGGGAAGCCAATAGTAGCATATAGAAGGCTAGG
TAAATTCTCCCCTAAAGATACCCTAGCCATAATGCAAAAATGTGAGCTACCCCCTCATAAATGA

Upstream 100 bases:

>100_bases
AAAGCCTTTTCCTAATTTATATTTAAAAGCTTAACTTACCATCAATTTAATACTATCATAAATAATAATAGTTATATACT
AGAATTGAGATAGTCTTCTT

Downstream 100 bases:

>100_bases
GGGAGCTTCCTGCTTCATAGCCCCACCTTGCCAGAGTACTGGTAGAGGGCGGAGCTCCACAGGCACTAAGGGTCGCTCCG
ACCCCGATCCACGTAAGATG

Product: Acetyl-CoA synthetase (acetate-CoA ligase) amino-end fragment. (acsA-2)

Products: NA

Alternate protein names: Acetate--CoA ligase; Acyl-activating enzyme [H]

Number of amino acids: Translated: 287; Mature: 287

Protein sequence:

>287_residues
MLEVDLREIRFHNYDELVRTFKWNIPSRFNIGESILDRKVKEGVGDNIAIYYEDEEGDHFVYTFAQLKSLSDSLITILRE
IGVKRGDVVGIYLQPRVETVISILSLYRLGAISLSISPLMGVEAVEYRIRQSDAKAIIIEGSRKEVREKLRNVTKIIVAD
EARDESEINFDEVKKTSGVHYAVDTKSDDPAQLFYTSGSTGAPKGVLHAHRFLLGHIPAYQLYFEMAPREEDVFYTPADW
GWIGAIGDVILPSLYFGKPIVAYRRLGKFSPKDTLAIMQKCELPPHK

Sequences:

>Translated_287_residues
MLEVDLREIRFHNYDELVRTFKWNIPSRFNIGESILDRKVKEGVGDNIAIYYEDEEGDHFVYTFAQLKSLSDSLITILRE
IGVKRGDVVGIYLQPRVETVISILSLYRLGAISLSISPLMGVEAVEYRIRQSDAKAIIIEGSRKEVREKLRNVTKIIVAD
EARDESEINFDEVKKTSGVHYAVDTKSDDPAQLFYTSGSTGAPKGVLHAHRFLLGHIPAYQLYFEMAPREEDVFYTPADW
GWIGAIGDVILPSLYFGKPIVAYRRLGKFSPKDTLAIMQKCELPPHK
>Mature_287_residues
MLEVDLREIRFHNYDELVRTFKWNIPSRFNIGESILDRKVKEGVGDNIAIYYEDEEGDHFVYTFAQLKSLSDSLITILRE
IGVKRGDVVGIYLQPRVETVISILSLYRLGAISLSISPLMGVEAVEYRIRQSDAKAIIIEGSRKEVREKLRNVTKIIVAD
EARDESEINFDEVKKTSGVHYAVDTKSDDPAQLFYTSGSTGAPKGVLHAHRFLLGHIPAYQLYFEMAPREEDVFYTPADW
GWIGAIGDVILPSLYFGKPIVAYRRLGKFSPKDTLAIMQKCELPPHK

Specific function: Enables The Cell To Use Acetate During Aerobic Growth To Generate Energy Via The Tca Cycle, And Biosynthetic Compounds Via The Glyoxylate Shunt. Acetylates Chey, The Response Regulator Involved In Flagellar Movement And Chemotaxis. [C]

COG id: COG0365

COG function: function code I; Acyl-coenzyme A synthetases/AMP-(fatty) acid ligases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI42544134, Length=283, Percent_Identity=29.3286219081272, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI42544132, Length=284, Percent_Identity=29.2253521126761, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI115511026, Length=240, Percent_Identity=30.4166666666667, Blast_Score=91, Evalue=1e-18,
Organism=Homo sapiens, GI122937307, Length=256, Percent_Identity=26.171875, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI157311624, Length=277, Percent_Identity=27.7978339350181, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI157311622, Length=277, Percent_Identity=27.7978339350181, Blast_Score=85, Evalue=6e-17,
Organism=Homo sapiens, GI28416953, Length=263, Percent_Identity=27.7566539923954, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI58082049, Length=277, Percent_Identity=26.7148014440433, Blast_Score=81, Evalue=9e-16,
Organism=Homo sapiens, GI38505220, Length=233, Percent_Identity=27.0386266094421, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI195947378, Length=268, Percent_Identity=24.6268656716418, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI13375727, Length=268, Percent_Identity=25.7462686567164, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI31982927, Length=290, Percent_Identity=27.2413793103448, Blast_Score=75, Evalue=6e-14,
Organism=Escherichia coli, GI1790505, Length=250, Percent_Identity=30, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI221142682, Length=202, Percent_Identity=24.7524752475248, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI145693145, Length=262, Percent_Identity=24.0458015267176, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71983001, Length=252, Percent_Identity=29.3650793650794, Blast_Score=92, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71982997, Length=252, Percent_Identity=29.3650793650794, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323182, Length=243, Percent_Identity=30.8641975308642, Blast_Score=95, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6319264, Length=258, Percent_Identity=27.5193798449612, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24667955, Length=292, Percent_Identity=25.6849315068493, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011904
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.1 [H]

Molecular weight: Translated: 32615; Mature: 32615

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEVDLREIRFHNYDELVRTFKWNIPSRFNIGESILDRKVKEGVGDNIAIYYEDEEGDHF
CCCCCHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCEE
VYTFAQLKSLSDSLITILREIGVKRGDVVGIYLQPRVETVISILSLYRLGAISLSISPLM
EEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHEEECCCHH
GVEAVEYRIRQSDAKAIIIEGSRKEVREKLRNVTKIIVADEARDESEINFDEVKKTSGVH
CHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHCCCCE
YAVDTKSDDPAQLFYTSGSTGAPKGVLHAHRFLLGHIPAYQLYFEMAPREEDVFYTPADW
EEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEECCCCC
GWIGAIGDVILPSLYFGKPIVAYRRLGKFSPKDTLAIMQKCELPPHK
CHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MLEVDLREIRFHNYDELVRTFKWNIPSRFNIGESILDRKVKEGVGDNIAIYYEDEEGDHF
CCCCCHHHHHCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCCEE
VYTFAQLKSLSDSLITILREIGVKRGDVVGIYLQPRVETVISILSLYRLGAISLSISPLM
EEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHEEECCCHH
GVEAVEYRIRQSDAKAIIIEGSRKEVREKLRNVTKIIVADEARDESEINFDEVKKTSGVH
CHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHCCCCE
YAVDTKSDDPAQLFYTSGSTGAPKGVLHAHRFLLGHIPAYQLYFEMAPREEDVFYTPADW
EEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCCEECCCCC
GWIGAIGDVILPSLYFGKPIVAYRRLGKFSPKDTLAIMQKCELPPHK
CHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572948; 12692562 [H]