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The map label for this gene is purK

Identifier: 15897933

GI number: 15897933

Start: 923215

End: 924312

Strand: Direct

Name: purK

Synonym: SSO1065

Alternate gene names: 15897933

Gene position: 923215-924312 (Clockwise)

Preceding gene: 15897932

Following gene: 15897934

Centisome position: 30.85

GC content: 35.79

Gene sequence:

>1098_bases
ATGTTCTCAGTACTAGACTGGAAGCCTAAAATTGGAATATTAGGAGGAGGACAGCTCGGCTGGATGATAGTATTAGAGGG
TAGAAAATACCCATTTACTTTTTACGTATTAGAGAACGATAAGAATGCTCCAGCTTGCAGAATTGCAGATAGGTGTTTCT
CTCCTCAAGATTATAAGGAATTCGTTGATTCCTCAGACGTTATAACATTTGAGTTCGAACACGTGTATGAAAAGGCATTA
GAGTATGCTGAGTATAGTGGCAAGCTATTACCTAGACTTAACTCTGTAGAGTTGAAGAGAGAGCGTTATAAGGAGAAGCT
GTTCTATAGACAACATAATTTACCAACTCCTAGATTCTATGTAGCAGAGGATGGTGAGGAAGCATTAAAGATATTAAGAG
AGGAATTCAATAATGTCGGAGTTATTAAGGAATCTAAAGGAGGATATGATGGTAAGGGGCAATATTTCATCTTTAATGAC
GTTGAGAAATATCAATTTCTAAGGGAAAAGAAAGAGAAGATGGTCGTTGAGGAGTATGTAAAATTTGATTTTGAGGCCTC
CATTATTATAGCAAGGGATAAGAGAGGTGTTTTTATTAGTTACCCTCCAACTTATAATTATAATGAAAAAGGTATTTTAG
TTTATAATTATGGGCCGTATAATAATCAGAATATAGTAGAGATTGCAAGAAGGTTAAGTGAGGAGTTGGATTACGTAGGA
ATTATGGGCGTTGAGGTATTCGTAGTTAACGGTAAAGTTTTAATTAATGAGTTTGCCCCAAGAGTTCACAATACTGGGCA
CTATACTCTTGACGGCGCTCTAATCTCTCAATTTGAACAACACCTAAGGGCAATAATCGGTATGGAGTTAGGTCCATCTA
CCATCTTATCTCCTAGCGGGATGGTTAATATTCTTGGTACAGATAAAATACCAGTTGAGGTATTAAAATACGGTAAAGTT
TACTGGTACTCTAAGAGTGAAGTTAGGAAGAGGAGAAAAATGGGTCATGTAAATGTAGTAGGGAACAATCTTGAAGAAGT
TAAGCAAAAAATTGATAAAATTATGCAACTAATCTATACTAATGGGTTAGATTTATGA

Upstream 100 bases:

>100_bases
GGTGCCAAAAACGCTGCGCTACTGGCAATTAGGATTTTAGGAATAAAATATAAGGAATTAGCAGATAAAATTAAAAAATT
CTCAGAGGATATGAGGAACG

Downstream 100 bases:

>100_bases
GGTTCAGATTTAAAATTTGGATTGAAGCAGAGGACGGAAAGCCACTTATAGGTAAAGGAGGGGTAAAATTACTAAGAGCG
ATTGAGGAAACCGGCTCTCT

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MFSVLDWKPKIGILGGGQLGWMIVLEGRKYPFTFYVLENDKNAPACRIADRCFSPQDYKEFVDSSDVITFEFEHVYEKAL
EYAEYSGKLLPRLNSVELKRERYKEKLFYRQHNLPTPRFYVAEDGEEALKILREEFNNVGVIKESKGGYDGKGQYFIFND
VEKYQFLREKKEKMVVEEYVKFDFEASIIIARDKRGVFISYPPTYNYNEKGILVYNYGPYNNQNIVEIARRLSEELDYVG
IMGVEVFVVNGKVLINEFAPRVHNTGHYTLDGALISQFEQHLRAIIGMELGPSTILSPSGMVNILGTDKIPVEVLKYGKV
YWYSKSEVRKRRKMGHVNVVGNNLEEVKQKIDKIMQLIYTNGLDL

Sequences:

>Translated_365_residues
MFSVLDWKPKIGILGGGQLGWMIVLEGRKYPFTFYVLENDKNAPACRIADRCFSPQDYKEFVDSSDVITFEFEHVYEKAL
EYAEYSGKLLPRLNSVELKRERYKEKLFYRQHNLPTPRFYVAEDGEEALKILREEFNNVGVIKESKGGYDGKGQYFIFND
VEKYQFLREKKEKMVVEEYVKFDFEASIIIARDKRGVFISYPPTYNYNEKGILVYNYGPYNNQNIVEIARRLSEELDYVG
IMGVEVFVVNGKVLINEFAPRVHNTGHYTLDGALISQFEQHLRAIIGMELGPSTILSPSGMVNILGTDKIPVEVLKYGKV
YWYSKSEVRKRRKMGHVNVVGNNLEEVKQKIDKIMQLIYTNGLDL
>Mature_365_residues
MFSVLDWKPKIGILGGGQLGWMIVLEGRKYPFTFYVLENDKNAPACRIADRCFSPQDYKEFVDSSDVITFEFEHVYEKAL
EYAEYSGKLLPRLNSVELKRERYKEKLFYRQHNLPTPRFYVAEDGEEALKILREEFNNVGVIKESKGGYDGKGQYFIFND
VEKYQFLREKKEKMVVEEYVKFDFEASIIIARDKRGVFISYPPTYNYNEKGILVYNYGPYNNQNIVEIARRLSEELDYVG
IMGVEVFVVNGKVLINEFAPRVHNTGHYTLDGALISQFEQHLRAIIGMELGPSTILSPSGMVNILGTDKIPVEVLKYGKV
YWYSKSEVRKRRKMGHVNVVGNNLEEVKQKIDKIMQLIYTNGLDL

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR)

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786733, Length=368, Percent_Identity=29.6195652173913, Blast_Score=157, Evalue=9e-40,
Organism=Escherichia coli, GI1788155, Length=358, Percent_Identity=25.6983240223464, Blast_Score=89, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324702, Length=378, Percent_Identity=30.4232804232804, Blast_Score=156, Evalue=5e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURK_SULSO (O06457)

Other databases:

- EMBL:   Y13143
- EMBL:   AE006641
- PIR:   A90259
- RefSeq:   NP_342538.1
- ProteinModelPortal:   O06457
- SMR:   O06457
- GeneID:   1454107
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO1065
- NMPDR:   fig|273057.1.peg.961
- HOGENOM:   HBG516369
- OMA:   LAVEMFV
- PhylomeDB:   O06457
- ProtClustDB:   PRK06019
- BioCyc:   SSOL273057:SSO1065-MONOMER
- BRENDA:   4.1.1.21
- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF1
- TIGRFAMs:   TIGR01161

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.18

Molecular weight: Translated: 42360; Mature: 42360

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 102-102 BINDING 143-143 BINDING 154-154 BINDING 185-185

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSVLDWKPKIGILGGGQLGWMIVLEGRKYPFTFYVLENDKNAPACRIADRCFSPQDYKE
CCCCCCCCCCEEEEECCCEEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHCCCCHHHHH
FVDSSDVITFEFEHVYEKALEYAEYSGKLLPRLNSVELKRERYKEKLFYRQHNLPTPRFY
HCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEE
VAEDGEEALKILREEFNNVGVIKESKGGYDGKGQYFIFNDVEKYQFLREKKEKMVVEEYV
EECCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHH
KFDFEASIIIARDKRGVFISYPPTYNYNEKGILVYNYGPYNNQNIVEIARRLSEELDYVG
HCCCCEEEEEEECCCCEEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCEEE
IMGVEVFVVNGKVLINEFAPRVHNTGHYTLDGALISQFEQHLRAIIGMELGPSTILSPSG
EEEEEEEEECCEEEEEHHCCHHCCCCCEEECHHHHHHHHHHHHHHHCCCCCCCCEECCCC
MVNILGTDKIPVEVLKYGKVYWYSKSEVRKRRKMGHVNVVGNNLEEVKQKIDKIMQLIYT
EEEEEECCCCCHHHHHCCCEEEECHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHH
NGLDL
CCCCC
>Mature Secondary Structure
MFSVLDWKPKIGILGGGQLGWMIVLEGRKYPFTFYVLENDKNAPACRIADRCFSPQDYKE
CCCCCCCCCCEEEEECCCEEEEEEECCCCCCEEEEEEECCCCCCHHHHHHHCCCCHHHHH
FVDSSDVITFEFEHVYEKALEYAEYSGKLLPRLNSVELKRERYKEKLFYRQHNLPTPRFY
HCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEE
VAEDGEEALKILREEFNNVGVIKESKGGYDGKGQYFIFNDVEKYQFLREKKEKMVVEEYV
EECCCHHHHHHHHHHHCCCCEEECCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHH
KFDFEASIIIARDKRGVFISYPPTYNYNEKGILVYNYGPYNNQNIVEIARRLSEELDYVG
HCCCCEEEEEEECCCCEEEECCCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCEEE
IMGVEVFVVNGKVLINEFAPRVHNTGHYTLDGALISQFEQHLRAIIGMELGPSTILSPSG
EEEEEEEEECCEEEEEHHCCHHCCCCCEEECHHHHHHHHHHHHHHHCCCCCCCCEECCCC
MVNILGTDKIPVEVLKYGKVYWYSKSEVRKRRKMGHVNVVGNNLEEVKQKIDKIMQLIYT
EEEEEECCCCCHHHHHCCCEEEECHHHHHHHHHCCCEEEECCCHHHHHHHHHHHHHHHHH
NGLDL
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9311113; 11427726