Click here to switch to the map view.

The map label for this gene is eiF2A [H]

Identifier: 15897919

GI number: 15897919

Start: 908305

End: 909105

Strand: Direct

Name: eiF2A [H]

Synonym: SSO1050

Alternate gene names: 15897919

Gene position: 908305-909105 (Clockwise)

Preceding gene: 15897918

Following gene: 15897920

Centisome position: 30.36

GC content: 34.58

Gene sequence:

>801_bases
ATGATTTACAGTAGAAGCAAACTACCCTCAGAAGGAGAAATTTTAATTGCAACTGTAAAACAAGTTTTTGATTATGGTAG
TTATGTTTCTTTAGACGAGTATGGGGGTTTACAGGCCTTTTTGCCTTGGAGTGAAGTAAGTAGCAAATGGGTTAAGAATA
TAAGGGATGTTCTCAAGGAAAATAGGAAAGTTATAGTAAAAGTAATTAGGGTTGATAGGAGAAAAGGTACTGTAGATGTG
TCATTAAAGAAAGTTACCGATGACGAGAGAAGAAAGAAGAATTTACAATGGAAGAAAATCCAACGATTAGATAAGATTTT
GGAACTAGTATCGCAAAAACTTAAATTAAGTGAAAAAGATGCGTGGGAACAAGTTGCGTGGAAATTAGAAGCCAAGTATG
GTGACCCTATTACAGCAATAGAAAAAGCTGTTAAAGAAGGAGAGAAAATACTAATTGATGCAGGAGTCCCAGAAATTTGG
GTTAAGCCATTACTAGAAGAGGCTTCAAAGCACGCTGAGGAAAGGAAAGTGAAAATGTCTGGTCTTATAACAGTTAGGAC
AAATGAGCCATTAGGAGTTGAAAAGATTAAAGAAGTGATCTCTAAGGCTTTAGAAAACATAGAACAAGACTATGAGAGCC
TATTAAATATTAAAATATATACTATTGGTGCCCCTAGATATAGGGTTGATGTAGTAGGTACTAATCCAAAGGAGGCCTCA
GAGGCACTAAATCAGATTATATCTAATTTAATTAAGATAGGAAAAGAAGAAAACGTAGATATAAGTGTGGTTAAGAAATG
A

Upstream 100 bases:

>100_bases
CATTTCCAGTTAGATGTCTAAGTTGTGGGACTGAGTTAGTATACTCTATGGGCGGTAAAGCAAAAATAGTTGGAGAAGTA
GTTAGAATAATGGGTTAAAG

Downstream 100 bases:

>100_bases
AGTGGAAAATGAGGAAATGTCCTAAAGACAACATCTATACTTTTAAAGATACTTGCCAAATATGTGGCTCTAAAACTGTG
ATCCCTCATCCATCCAGATT

Product: translation initiation factor IF-2 subunit alpha

Products: RNAn; a nucleoside diphosphate [C]

Alternate protein names: aIF2-alpha; eIF-2-alpha [H]

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MIYSRSKLPSEGEILIATVKQVFDYGSYVSLDEYGGLQAFLPWSEVSSKWVKNIRDVLKENRKVIVKVIRVDRRKGTVDV
SLKKVTDDERRKKNLQWKKIQRLDKILELVSQKLKLSEKDAWEQVAWKLEAKYGDPITAIEKAVKEGEKILIDAGVPEIW
VKPLLEEASKHAEERKVKMSGLITVRTNEPLGVEKIKEVISKALENIEQDYESLLNIKIYTIGAPRYRVDVVGTNPKEAS
EALNQIISNLIKIGKEENVDISVVKK

Sequences:

>Translated_266_residues
MIYSRSKLPSEGEILIATVKQVFDYGSYVSLDEYGGLQAFLPWSEVSSKWVKNIRDVLKENRKVIVKVIRVDRRKGTVDV
SLKKVTDDERRKKNLQWKKIQRLDKILELVSQKLKLSEKDAWEQVAWKLEAKYGDPITAIEKAVKEGEKILIDAGVPEIW
VKPLLEEASKHAEERKVKMSGLITVRTNEPLGVEKIKEVISKALENIEQDYESLLNIKIYTIGAPRYRVDVVGTNPKEAS
EALNQIISNLIKIGKEENVDISVVKK
>Mature_266_residues
MIYSRSKLPSEGEILIATVKQVFDYGSYVSLDEYGGLQAFLPWSEVSSKWVKNIRDVLKENRKVIVKVIRVDRRKGTVDV
SLKKVTDDERRKKNLQWKKIQRLDKILELVSQKLKLSEKDAWEQVAWKLEAKYGDPITAIEKAVKEGEKILIDAGVPEIW
VKPLLEEASKHAEERKVKMSGLITVRTNEPLGVEKIKEVISKALENIEQDYESLLNIKIYTIGAPRYRVDVVGTNPKEAS
EALNQIISNLIKIGKEENVDISVVKK

Specific function: eIF-2 functions in the early steps of protein synthesis by forming a ternary complex with GTP and initiator tRNA [H]

COG id: COG1093

COG function: function code J; Translation initiation factor 2, alpha subunit (eIF-2alpha)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Homo sapiens, GI4758256, Length=261, Percent_Identity=25.6704980842912, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17509871, Length=257, Percent_Identity=24.5136186770428, Blast_Score=78, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6322466, Length=258, Percent_Identity=23.2558139534884, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI18860087, Length=254, Percent_Identity=23.2283464566929, Blast_Score=66, Evalue=3e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011488
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR022964 [H]

Pfam domain/function: PF07541 EIF_2_alpha; PF00575 S1 [H]

EC number: 2.7.7.8 [C]

Molecular weight: Translated: 30383; Mature: 30383

Theoretical pI: Translated: 9.81; Mature: 9.81

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYSRSKLPSEGEILIATVKQVFDYGSYVSLDEYGGLQAFLPWSEVSSKWVKNIRDVLKE
CCCCCCCCCCCCCCHHHHHHHHHCCCCEECCHHCCCEEEECCHHHHHHHHHHHHHHHHHH
NRKVIVKVIRVDRRKGTVDVSLKKVTDDERRKKNLQWKKIQRLDKILELVSQKLKLSEKD
HHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHH
AWEQVAWKLEAKYGDPITAIEKAVKEGEKILIDAGVPEIWVKPLLEEASKHAEERKVKMS
HHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
GLITVRTNEPLGVEKIKEVISKALENIEQDYESLLNIKIYTIGAPRYRVDVVGTNPKEAS
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCEEEEEECCCHHHHH
EALNQIISNLIKIGKEENVDISVVKK
HHHHHHHHHHHHCCCCCCCCEEEECC
>Mature Secondary Structure
MIYSRSKLPSEGEILIATVKQVFDYGSYVSLDEYGGLQAFLPWSEVSSKWVKNIRDVLKE
CCCCCCCCCCCCCCHHHHHHHHHCCCCEECCHHCCCEEEECCHHHHHHHHHHHHHHHHHH
NRKVIVKVIRVDRRKGTVDVSLKKVTDDERRKKNLQWKKIQRLDKILELVSQKLKLSEKD
HHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHH
AWEQVAWKLEAKYGDPITAIEKAVKEGEKILIDAGVPEIWVKPLLEEASKHAEERKVKMS
HHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
GLITVRTNEPLGVEKIKEVISKALENIEQDYESLLNIKIYTIGAPRYRVDVVGTNPKEAS
EEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCEEEEEECCCHHHHH
EALNQIISNLIKIGKEENVDISVVKK
HHHHHHHHHHHHCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 4500 [C]

Specific activity: NA

Km value (mM): NA

Substrates: RNAn+1; phosphate [C]

Specific reaction: RNAn+1 + phosphate = RNAn + a nucleoside diphosphate [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA