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The map label for this gene is gcvH

Identifier: 15897807

GI number: 15897807

Start: 783434

End: 783847

Strand: Direct

Name: gcvH

Synonym: SSO0920

Alternate gene names: 15897807

Gene position: 783434-783847 (Clockwise)

Preceding gene: 15897806

Following gene: 15897808

Centisome position: 26.18

GC content: 35.75

Gene sequence:

>414_bases
ATGAATGAAATGAAAGTAGGTAGATACGTAGTTCTGACAGATAGATTATACACAGAGACTGACGAATGGGTGGTGCTTTC
TAATGATAACGTGGCAGTAATTGGAATAACTGATTACGCACAAAAGAAGTTAAGAGATATAGTAGGAATTGAATTACCTC
AACTGCAGAAGGAAGTGAAAGCTGGAGAATCTGTAGGAGTTATAGAATCTGTTAAAGCTGCTGCAGATATTTTCTCTCCA
TTAAGTGGGATAATAGTTGAGGTAAACAATAAATTACTTGAGCATCCAGAGATAATAAATAAGGATCCGTATGGTGAAGG
CTGGATTTTCAAGTTAAAGGCGTCTAAGTTATCTGAAGAAAAAGAAAAACTACTTAGCCCGGAAAAGTATATTGAAAAAA
TAAAGGGTGGGTAG

Upstream 100 bases:

>100_bases
CCAAGTACTTCTACCTCGGCTATAGTTTAACTGGCGAAATTAGGGGTAAACAGTATGATATAAAAATCGATGAGTTTCCA
TTTATCTAGGTGAATATTAT

Downstream 100 bases:

>100_bases
ATTGAGCGAGGTATTTGAAGTTAGGGAAAGATATGTTCCCCAGATAGGTAGACCCTCATATTTATTAGTGAGCTTACCAG
ATGCCGGATTAGTAGGTAGT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 137; Mature: 137

Protein sequence:

>137_residues
MNEMKVGRYVVLTDRLYTETDEWVVLSNDNVAVIGITDYAQKKLRDIVGIELPQLQKEVKAGESVGVIESVKAAADIFSP
LSGIIVEVNNKLLEHPEIINKDPYGEGWIFKLKASKLSEEKEKLLSPEKYIEKIKGG

Sequences:

>Translated_137_residues
MNEMKVGRYVVLTDRLYTETDEWVVLSNDNVAVIGITDYAQKKLRDIVGIELPQLQKEVKAGESVGVIESVKAAADIFSP
LSGIIVEVNNKLLEHPEIINKDPYGEGWIFKLKASKLSEEKEKLLSPEKYIEKIKGG
>Mature_137_residues
MNEMKVGRYVVLTDRLYTETDEWVVLSNDNVAVIGITDYAQKKLRDIVGIELPQLQKEVKAGESVGVIESVKAAADIFSP
LSGIIVEVNNKLLEHPEIINKDPYGEGWIFKLKASKLSEEKEKLLSPEKYIEKIKGG

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI49574537, Length=126, Percent_Identity=39.6825396825397, Blast_Score=96, Evalue=7e-21,
Organism=Homo sapiens, GI89057342, Length=126, Percent_Identity=38.8888888888889, Blast_Score=93, Evalue=7e-20,
Organism=Escherichia coli, GI1789271, Length=114, Percent_Identity=46.4912280701754, Blast_Score=106, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI17507493, Length=118, Percent_Identity=48.3050847457627, Blast_Score=113, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17551294, Length=116, Percent_Identity=47.4137931034483, Blast_Score=111, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319272, Length=116, Percent_Identity=42.2413793103448, Blast_Score=103, Evalue=8e-24,
Organism=Drosophila melanogaster, GI17865652, Length=117, Percent_Identity=37.6068376068376, Blast_Score=91, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH1_SULSO (Q97ZI6)

Other databases:

- EMBL:   AE006641
- PIR:   C90243
- RefSeq:   NP_342412.1
- ProteinModelPortal:   Q97ZI6
- SMR:   Q97ZI6
- GeneID:   1455169
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0920
- NMPDR:   fig|273057.1.peg.835
- HOGENOM:   HBG693789
- OMA:   SASEYEQ
- PhylomeDB:   Q97ZI6
- ProtClustDB:   PRK01202
- BioCyc:   SSOL273057:SSO0920-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 15345; Mature: 15345

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEMKVGRYVVLTDRLYTETDEWVVLSNDNVAVIGITDYAQKKLRDIVGIELPQLQKEVK
CCCCCCCCEEEEECCEECCCCCEEEECCCCEEEEEECHHHHHHHHHHHCCCCHHHHHHHH
AGESVGVIESVKAAADIFSPLSGIIVEVNNKLLEHPEIINKDPYGEGWIFKLKASKLSEE
CCCCCCHHHHHHHHHHHHHHHHHEEEEECCHHHCCCCCCCCCCCCCCEEEEEEHHHHHHH
KEKLLSPEKYIEKIKGG
HHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MNEMKVGRYVVLTDRLYTETDEWVVLSNDNVAVIGITDYAQKKLRDIVGIELPQLQKEVK
CCCCCCCCEEEEECCEECCCCCEEEECCCCEEEEEECHHHHHHHHHHHCCCCHHHHHHHH
AGESVGVIESVKAAADIFSPLSGIIVEVNNKLLEHPEIINKDPYGEGWIFKLKASKLSEE
CCCCCCHHHHHHHHHHHHHHHHHEEEEECCHHHCCCCCCCCCCCCCCEEEEEEHHHHHHH
KEKLLSPEKYIEKIKGG
HHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726