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The map label for this gene is rfcL [H]

Identifier: 15897671

GI number: 15897671

Start: 654081

End: 655298

Strand: Direct

Name: rfcL [H]

Synonym: SSO0769

Alternate gene names: 15897671

Gene position: 654081-655298 (Clockwise)

Preceding gene: 15897670

Following gene: 15897675

Centisome position: 21.86

GC content: 33.5

Gene sequence:

>1218_bases
ATGATACAATGGTTTTTAAAATATAGGCCGCGTTCTCTAAAAGATGTGGAGAATCAAGATGATGCGAAAAAGCAACTACA
GGAGTGGATAGAGTCTTGGCTTAACGGAAATTCTAACGTTAAGGCTGTACTATTACACGGTCCTCCAGGTGTTGGAAAAA
CTGTCTTAGCTGAGGCATTAGCTCACGATTATAATTTTGAGTTATTAGAAATGAACGCTAGCGATTCAAGAAAATTGCAA
GATATAAAGAGCATAGCGGAAAAGGCAGCAGTTTATGGCAGTATATTTGGAACAAAGGGTAAATTAATACTTCTAGATGA
GGTTGACGGAATAAATGTTCGTGAAGATACTGGAGCTATTCAAGGTATTTTAGAACTTATTGAAAAGACAAAATATCCTA
TTATAATGACAGCTAATGATCCATGGAATCCTGGATTAAGAGAATTAAGAAATAAGGCAAAAATGATCGAATTAAGTAAG
TTAGGTAAGTATCCTTTAAGAAGAATTTTAAAAAAGATTTGCCAAGCAGAAAAGATTATTTGTGACGATGAGGCCTTAAA
TTATATAATTGATAGCAGTGAAGGAGATGCTAGATATGCAATTAATATTTTGCAAGGAATTGGGGAAGGATACGGAAAGG
TTACTCTTAATTTGGTTGAATCATTAGCTAAAAGAAAAGAACGTGAATTAGACCCATTTGAAACGCTAAGAGATATATTC
TGGGCAAGATATGCATGGCAAGCTAAAAATGCAGCTACAAGTGCTCAAATAGATTATGATATGCTAATCAGATGGATATC
AGAAAACATACCTATACAATACGATAATATTGAAGATATTTGGAGAGCTTTTGATGCTCTCTCTAGAGCTTCAATATTCC
TAAAGAGAGCAAAAAGCGGTGATTGGGACTTATTATCTTACGCGTATGATATGATGAGCTCTGGAGTAGCTTTTGCAGAA
CCTGAAAAGAAAAAGCCAAATTGGAAACCAAAATGGAAAAAATATCAATTCCCGTCGTATATACAACTACTATCTAAGAG
TAAAGATATAAGGGATACAAGGGACGAAATCATTAAGAAACTAGCAATACATTCATCCTTTAATAAGGCACTAAATGACA
CATATCCATTTTTCCTACTATTCTATAAAAAATACGATAAACATCTTAATTTAAATACGAAAGAAAAGGAGTATCTTAGT
TCCATATCTAAATCTTAG

Upstream 100 bases:

>100_bases
AGTTTAGAATAATAGAAGGTGCTGACGATGAAATTCAGTTATCTGCATTATTAGCTAAAATGGCAATTTATGGAAATAAG
TATATAGGCAGTGAGAATAG

Downstream 100 bases:

>100_bases
TCTTTCTCCTTTATTTTATCTATTACTTTTATATCATCAATCATGGTATATGGATATTTTCCGTTCTCATCTTTTTCATA
TTTTTTTACCATATCCCAAA

Product: replication factor C large subunit

Products: NA

Alternate protein names: RFC large subunit; Clamp loader large subunit [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MIQWFLKYRPRSLKDVENQDDAKKQLQEWIESWLNGNSNVKAVLLHGPPGVGKTVLAEALAHDYNFELLEMNASDSRKLQ
DIKSIAEKAAVYGSIFGTKGKLILLDEVDGINVREDTGAIQGILELIEKTKYPIIMTANDPWNPGLRELRNKAKMIELSK
LGKYPLRRILKKICQAEKIICDDEALNYIIDSSEGDARYAINILQGIGEGYGKVTLNLVESLAKRKERELDPFETLRDIF
WARYAWQAKNAATSAQIDYDMLIRWISENIPIQYDNIEDIWRAFDALSRASIFLKRAKSGDWDLLSYAYDMMSSGVAFAE
PEKKKPNWKPKWKKYQFPSYIQLLSKSKDIRDTRDEIIKKLAIHSSFNKALNDTYPFFLLFYKKYDKHLNLNTKEKEYLS
SISKS

Sequences:

>Translated_405_residues
MIQWFLKYRPRSLKDVENQDDAKKQLQEWIESWLNGNSNVKAVLLHGPPGVGKTVLAEALAHDYNFELLEMNASDSRKLQ
DIKSIAEKAAVYGSIFGTKGKLILLDEVDGINVREDTGAIQGILELIEKTKYPIIMTANDPWNPGLRELRNKAKMIELSK
LGKYPLRRILKKICQAEKIICDDEALNYIIDSSEGDARYAINILQGIGEGYGKVTLNLVESLAKRKERELDPFETLRDIF
WARYAWQAKNAATSAQIDYDMLIRWISENIPIQYDNIEDIWRAFDALSRASIFLKRAKSGDWDLLSYAYDMMSSGVAFAE
PEKKKPNWKPKWKKYQFPSYIQLLSKSKDIRDTRDEIIKKLAIHSSFNKALNDTYPFFLLFYKKYDKHLNLNTKEKEYLS
SISKS
>Mature_405_residues
MIQWFLKYRPRSLKDVENQDDAKKQLQEWIESWLNGNSNVKAVLLHGPPGVGKTVLAEALAHDYNFELLEMNASDSRKLQ
DIKSIAEKAAVYGSIFGTKGKLILLDEVDGINVREDTGAIQGILELIEKTKYPIIMTANDPWNPGLRELRNKAKMIELSK
LGKYPLRRILKKICQAEKIICDDEALNYIIDSSEGDARYAINILQGIGEGYGKVTLNLVESLAKRKERELDPFETLRDIF
WARYAWQAKNAATSAQIDYDMLIRWISENIPIQYDNIEDIWRAFDALSRASIFLKRAKSGDWDLLSYAYDMMSSGVAFAE
PEKKKPNWKPKWKKYQFPSYIQLLSKSKDIRDTRDEIIKKLAIHSSFNKALNDTYPFFLLFYKKYDKHLNLNTKEKEYLS
SISKS

Specific function: Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the activator 1 small subunits family. RfcL subfamily [H]

Homologues:

Organism=Homo sapiens, GI32528306, Length=421, Percent_Identity=25.6532066508314, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI6677723, Length=221, Percent_Identity=29.4117647058824, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI194306571, Length=216, Percent_Identity=29.6296296296296, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI194306567, Length=216, Percent_Identity=29.6296296296296, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI31563534, Length=224, Percent_Identity=26.7857142857143, Blast_Score=72, Evalue=7e-13,
Organism=Homo sapiens, GI4506491, Length=264, Percent_Identity=26.8939393939394, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI31881687, Length=264, Percent_Identity=26.8939393939394, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17563226, Length=352, Percent_Identity=27.2727272727273, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17541368, Length=172, Percent_Identity=31.3953488372093, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17541988, Length=215, Percent_Identity=28.8372093023256, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17554730, Length=228, Percent_Identity=27.1929824561404, Blast_Score=76, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6324791, Length=225, Percent_Identity=27.5555555555556, Blast_Score=93, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6324478, Length=224, Percent_Identity=29.4642857142857, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323724, Length=255, Percent_Identity=25.0980392156863, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324039, Length=216, Percent_Identity=27.3148148148148, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6322528, Length=251, Percent_Identity=29.4820717131474, Blast_Score=73, Evalue=8e-14,
Organism=Drosophila melanogaster, GI17737765, Length=345, Percent_Identity=26.3768115942029, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI62472391, Length=285, Percent_Identity=27.719298245614, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI17647857, Length=222, Percent_Identity=25.2252252252252, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28574716, Length=203, Percent_Identity=28.5714285714286, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19921076, Length=218, Percent_Identity=25.6880733944954, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI18859927, Length=218, Percent_Identity=28.4403669724771, Blast_Score=69, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 46787; Mature: 46787

Theoretical pI: Translated: 9.24; Mature: 9.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQWFLKYRPRSLKDVENQDDAKKQLQEWIESWLNGNSNVKAVLLHGPPGVGKTVLAEAL
CCEEEECCCCCCHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHH
AHDYNFELLEMNASDSRKLQDIKSIAEKAAVYGSIFGTKGKLILLDEVDGINVREDTGAI
HCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHH
QGILELIEKTKYPIIMTANDPWNPGLRELRNKAKMIELSKLGKYPLRRILKKICQAEKII
HHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCC
CDDEALNYIIDSSEGDARYAINILQGIGEGYGKVTLNLVESLAKRKERELDPFETLRDIF
CCHHHHHHEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
WARYAWQAKNAATSAQIDYDMLIRWISENIPIQYDNIEDIWRAFDALSRASIFLKRAKSG
HHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCC
DWDLLSYAYDMMSSGVAFAEPEKKKPNWKPKWKKYQFPSYIQLLSKSKDIRDTRDEIIKK
CHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHH
LAIHSSFNKALNDTYPFFLLFYKKYDKHLNLNTKEKEYLSSISKS
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MIQWFLKYRPRSLKDVENQDDAKKQLQEWIESWLNGNSNVKAVLLHGPPGVGKTVLAEAL
CCEEEECCCCCCHHCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHH
AHDYNFELLEMNASDSRKLQDIKSIAEKAAVYGSIFGTKGKLILLDEVDGINVREDTGAI
HCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHH
QGILELIEKTKYPIIMTANDPWNPGLRELRNKAKMIELSKLGKYPLRRILKKICQAEKII
HHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCC
CDDEALNYIIDSSEGDARYAINILQGIGEGYGKVTLNLVESLAKRKERELDPFETLRDIF
CCHHHHHHEEECCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
WARYAWQAKNAATSAQIDYDMLIRWISENIPIQYDNIEDIWRAFDALSRASIFLKRAKSG
HHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCC
DWDLLSYAYDMMSSGVAFAEPEKKKPNWKPKWKKYQFPSYIQLLSKSKDIRDTRDEIIKK
CHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHH
LAIHSSFNKALNDTYPFFLLFYKKYDKHLNLNTKEKEYLSSISKS
HHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Metalloendopeptidases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA