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The map label for this gene is rfc

Identifier: 15897670

GI number: 15897670

Start: 653092

End: 654084

Strand: Direct

Name: rfc

Synonym: SSO0768

Alternate gene names: 15897670

Gene position: 653092-654084 (Clockwise)

Preceding gene: 15897669

Following gene: 15897671

Centisome position: 21.83

GC content: 35.15

Gene sequence:

>993_bases
ATGAGCACGAAGGTCGAAGAAATACTTTGGGCTGAAAAATATAGACCTAAGACGTTAGATGATATAGTAAATCAAAGAGA
GATCATAGATAGGTTAAAAAAATTTGTCAAGGAAAAGAATATGCCTCATCTACTTTTTGCTGGGCCACCGGGTACCGGAA
AAACTACCGCTGCTTTAGCTCTTGTACATGACTTATATGGAGATAATTATACAGAATATTTCCTCGAGCTAAATGCAAGC
GATGAAAGAGGAATAGATGTAATTAGAAATAAAGTAAAGGAATTCGCTCGTACGGTAATTCCAGGTGATATTCCATTTAA
GGTGGTTCTTTTAGATGAAGCAGACAACATGACCGCTGATGCCCAACAAGCTTTAAGGAGAACTATGGAGCTGTATACAG
AAAACACTAGATTTATTCTAGCTTGCAATTATTTAAGTAAAATCATTGAGCCAATACAATCTAGAACTGCATTATTTAGA
TTTTACCCGCTGAAAAAGGAAGATGTCGTTAATAGATTAATCTATATAGCTAAAAATGAGAAAGCTGAATACGATCAGAA
AGCTTTGGAAACGATCTACGATATAACTATGGGAGATATGAGAAAAAGTATAAACATTTTACAAGCCGCCTCAGCTTATG
GCAAGATTAGTGTAGAGGCCGTATTTAAGGTTTTAGGATTAGCACAGCCTAAGGAAGTTAGAGAGATGATTAACTTAGCT
CTGCAAGGTAAGTTCACACAAGCAAGAGATAAACTCAGAACTTTACTTATTACATATGGCCTATCTGGAGAGGATATAGT
AAAGCAAATACACAGAGAGATAACTTCCTCAGAGATTCAAATAAGTGAAGAGTTGAGAGTTTTACTACTAGATTATATAG
GAGAGACAGAGTTTAGAATAATAGAAGGTGCTGACGATGAAATTCAGTTATCTGCATTATTAGCTAAAATGGCAATTTAT
GGAAATAAGTATATAGGCAGTGAGAATAGATGA

Upstream 100 bases:

>100_bases
TGCTGATAAGTTAAAATCACTAATGGAGGGACATGGGATAGAGAGAGGATTCGTAATGGGTAAATATAAGAGAAAATAAT
TTCACTTATGTGGTGAGATA

Downstream 100 bases:

>100_bases
TACAATGGTTTTTAAAATATAGGCCGCGTTCTCTAAAAGATGTGGAGAATCAAGATGATGCGAAAAAGCAACTACAGGAG
TGGATAGAGTCTTGGCTTAA

Product: replication factor C small subunit

Products: N Pyrophosphate; DNA(N). [C]

Alternate protein names: RFC small subunit; Clamp loader small subunit; SsoRFC small subunit

Number of amino acids: Translated: 330; Mature: 329

Protein sequence:

>330_residues
MSTKVEEILWAEKYRPKTLDDIVNQREIIDRLKKFVKEKNMPHLLFAGPPGTGKTTAALALVHDLYGDNYTEYFLELNAS
DERGIDVIRNKVKEFARTVIPGDIPFKVVLLDEADNMTADAQQALRRTMELYTENTRFILACNYLSKIIEPIQSRTALFR
FYPLKKEDVVNRLIYIAKNEKAEYDQKALETIYDITMGDMRKSINILQAASAYGKISVEAVFKVLGLAQPKEVREMINLA
LQGKFTQARDKLRTLLITYGLSGEDIVKQIHREITSSEIQISEELRVLLLDYIGETEFRIIEGADDEIQLSALLAKMAIY
GNKYIGSENR

Sequences:

>Translated_330_residues
MSTKVEEILWAEKYRPKTLDDIVNQREIIDRLKKFVKEKNMPHLLFAGPPGTGKTTAALALVHDLYGDNYTEYFLELNAS
DERGIDVIRNKVKEFARTVIPGDIPFKVVLLDEADNMTADAQQALRRTMELYTENTRFILACNYLSKIIEPIQSRTALFR
FYPLKKEDVVNRLIYIAKNEKAEYDQKALETIYDITMGDMRKSINILQAASAYGKISVEAVFKVLGLAQPKEVREMINLA
LQGKFTQARDKLRTLLITYGLSGEDIVKQIHREITSSEIQISEELRVLLLDYIGETEFRIIEGADDEIQLSALLAKMAIY
GNKYIGSENR
>Mature_329_residues
STKVEEILWAEKYRPKTLDDIVNQREIIDRLKKFVKEKNMPHLLFAGPPGTGKTTAALALVHDLYGDNYTEYFLELNASD
ERGIDVIRNKVKEFARTVIPGDIPFKVVLLDEADNMTADAQQALRRTMELYTENTRFILACNYLSKIIEPIQSRTALFRF
YPLKKEDVVNRLIYIAKNEKAEYDQKALETIYDITMGDMRKSINILQAASAYGKISVEAVFKVLGLAQPKEVREMINLAL
QGKFTQARDKLRTLLITYGLSGEDIVKQIHREITSSEIQISEELRVLLLDYIGETEFRIIEGADDEIQLSALLAKMAIYG
NKYIGSENR

Specific function: Part of the RFC clamp loader complex which loads the PCNA sliding clamp onto DNA. The complex possesses DNA-dependent ATPase activity

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the activator 1 small subunits family. RfcS subfamily

Homologues:

Organism=Homo sapiens, GI6677723, Length=320, Percent_Identity=41.25, Blast_Score=261, Evalue=7e-70,
Organism=Homo sapiens, GI31563534, Length=316, Percent_Identity=43.0379746835443, Blast_Score=251, Evalue=5e-67,
Organism=Homo sapiens, GI194306571, Length=310, Percent_Identity=41.6129032258065, Blast_Score=251, Evalue=6e-67,
Organism=Homo sapiens, GI194306567, Length=310, Percent_Identity=41.6129032258065, Blast_Score=251, Evalue=6e-67,
Organism=Homo sapiens, GI4506491, Length=326, Percent_Identity=40.1840490797546, Blast_Score=225, Evalue=3e-59,
Organism=Homo sapiens, GI31881687, Length=326, Percent_Identity=40.1840490797546, Blast_Score=225, Evalue=3e-59,
Organism=Homo sapiens, GI28882049, Length=313, Percent_Identity=38.0191693290735, Blast_Score=205, Evalue=4e-53,
Organism=Homo sapiens, GI194306569, Length=246, Percent_Identity=40.650406504065, Blast_Score=186, Evalue=2e-47,
Organism=Homo sapiens, GI4506489, Length=231, Percent_Identity=32.4675324675325, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI108773789, Length=231, Percent_Identity=32.4675324675325, Blast_Score=120, Evalue=2e-27,
Organism=Escherichia coli, GI1786676, Length=238, Percent_Identity=27.3109243697479, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17541988, Length=315, Percent_Identity=44.7619047619048, Blast_Score=256, Evalue=8e-69,
Organism=Caenorhabditis elegans, GI71986063, Length=320, Percent_Identity=39.0625, Blast_Score=242, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI17554730, Length=208, Percent_Identity=44.2307692307692, Blast_Score=188, Evalue=3e-48,
Organism=Caenorhabditis elegans, GI17541990, Length=233, Percent_Identity=35.6223175965665, Blast_Score=123, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6324478, Length=321, Percent_Identity=45.7943925233645, Blast_Score=273, Evalue=3e-74,
Organism=Saccharomyces cerevisiae, GI6324039, Length=318, Percent_Identity=39.622641509434, Blast_Score=243, Evalue=4e-65,
Organism=Saccharomyces cerevisiae, GI6322528, Length=328, Percent_Identity=40.8536585365854, Blast_Score=233, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6319562, Length=248, Percent_Identity=31.0483870967742, Blast_Score=117, Evalue=2e-27,
Organism=Drosophila melanogaster, GI17647857, Length=325, Percent_Identity=45.5384615384615, Blast_Score=262, Evalue=2e-70,
Organism=Drosophila melanogaster, GI19921076, Length=315, Percent_Identity=37.4603174603175, Blast_Score=218, Evalue=6e-57,
Organism=Drosophila melanogaster, GI18859927, Length=317, Percent_Identity=36.2776025236593, Blast_Score=211, Evalue=4e-55,
Organism=Drosophila melanogaster, GI19921136, Length=346, Percent_Identity=28.0346820809249, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RFCS_SULSO (Q9UXF5)

Other databases:

- EMBL:   Y18930
- EMBL:   AE006641
- PIR:   B90226
- RefSeq:   NP_342275.1
- ProteinModelPortal:   Q9UXF5
- SMR:   Q9UXF5
- GeneID:   1455032
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0768
- NMPDR:   fig|273057.1.peg.698
- HOGENOM:   HBG541599
- OMA:   CDFRIVE
- PhylomeDB:   Q9UXF5
- ProtClustDB:   PRK00440
- BioCyc:   SSOL273057:SSO0768-MONOMER
- HAMAP:   MF_01509
- InterPro:   IPR003593
- InterPro:   IPR003959
- InterPro:   IPR008921
- InterPro:   IPR013748
- SMART:   SM00382

Pfam domain/function: PF00004 AAA; PF08542 Rep_fac_C; SSF48019 Pol_clamp_load_C

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 37741; Mature: 37610

Theoretical pI: Translated: 5.59; Mature: 5.59

Prosite motif: PS00039 DEAD_ATP_HELICASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKVEEILWAEKYRPKTLDDIVNQREIIDRLKKFVKEKNMPHLLFAGPPGTGKTTAALA
CCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHH
LVHDLYGDNYTEYFLELNASDERGIDVIRNKVKEFARTVIPGDIPFKVVLLDEADNMTAD
HHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHH
AQQALRRTMELYTENTRFILACNYLSKIIEPIQSRTALFRFYPLKKEDVVNRLIYIAKNE
HHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEECCC
KAEYDQKALETIYDITMGDMRKSINILQAASAYGKISVEAVFKVLGLAQPKEVREMINLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHH
LQGKFTQARDKLRTLLITYGLSGEDIVKQIHREITSSEIQISEELRVLLLDYIGETEFRI
HCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IEGADDEIQLSALLAKMAIYGNKYIGSENR
EECCCCHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
STKVEEILWAEKYRPKTLDDIVNQREIIDRLKKFVKEKNMPHLLFAGPPGTGKTTAALA
CCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHH
LVHDLYGDNYTEYFLELNASDERGIDVIRNKVKEFARTVIPGDIPFKVVLLDEADNMTAD
HHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHH
AQQALRRTMELYTENTRFILACNYLSKIIEPIQSRTALFRFYPLKKEDVVNRLIYIAKNE
HHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEEECCC
KAEYDQKALETIYDITMGDMRKSINILQAASAYGKISVEAVFKVLGLAQPKEVREMINLA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHH
LQGKFTQARDKLRTLLITYGLSGEDIVKQIHREITSSEIQISEELRVLLLDYIGETEFRI
HCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IEGADDEIQLSALLAKMAIYGNKYIGSENR
EECCCCHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: N Deoxynucleoside Triphosphate; DNA [C]

Specific reaction: N Deoxynucleoside Triphosphate = N Pyrophosphate + DNA(N). Protein + DNA = Protein-DNA [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10701121; 11427726