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The map label for this gene is gatA-1 [H]

Identifier: 15897667

GI number: 15897667

Start: 650696

End: 651892

Strand: Direct

Name: gatA-1 [H]

Synonym: SSO0765

Alternate gene names: 15897667

Gene position: 650696-651892 (Clockwise)

Preceding gene: 15897663

Following gene: 15897668

Centisome position: 21.75

GC content: 34.59

Gene sequence:

>1197_bases
ATGAAATTAGAAGAATTAAATTCAAAATATAATGCATTTATAACCCTCCATACTATCGAAGAAGGAAACGATAAAGGCAA
ATTAAAGGATGTTACATTCGGAATAAAGGATGTTATTTTAACTAAGGACGTAAGGACCACTGCTGGATCAAGAGTTTTAG
AAAACTATGTTCCACAAAGAAACGCATACGTTGTGGATGCTATACTTAGAGAAGGAGGAAAGATAATAGGAAAAACTAAT
ACTCACGAATTTGCGCTAGGTGCTACTAATACATCAAGTATAGGAGGACCTGCAAGAAATCCTGTTGATCCAGAAAGAAT
AAGCGGTGGTTCTAGTGGCGGTTCCGCTGTTGCGGTAAAGTTAGATATGGTCGACGTGGGAATTGGTACTGATACCGGAG
GATCTGTAAGGATCCCTTCGTCTTTATGTGGAGTAATTGGATTTAAACCAACTACTGGGTTGATACCTAATGATGGCGTA
ATACCCTTTAGCTGGTCATTAGATACTGTGGGGATAATCGTAAAAGATAACATTAGATTATTACGAAGAGTTTTTGACGT
TGTTCTGCCAAATGAAAAAAGAAAAGTTGAAATAGCTAAATTAAGAACAAGACCGCGTCTAGGTTTATTTCTATTCGATG
ATATGGAAGTTTCAAGAATATTGCTGAAAGATATATATGCTAAATTATCTTCACACTTTGATATAGTCGAGGTTGATCTT
CCTTTACTAAGGCAATATGGCTCTAAAACTAGAAGAACTATATCATTAGCTGAAGCTGCTTCTTATCATAAGGATTGGAT
AAGTGAATATAGTGATAAATACTTCAAAGATACCTATACCTTACTCCTTGATGGTATGAAAATATCTGCTACCGACTATA
TAGATGCGCTTAGACATAGGAGAGTATTAATAGAAGAGTATGTAAAAGTATTTAAGAATATTGATTTCATTTTATCTCCT
ACAACAAAAATTGTTGCACCTAGAATTTCTGACGTAATCTCTAACCCTCTTCAATTTAGAGAATATCTAATAGCTAATAC
TGAACTATTTAATGTAGTTGGAGCACCATCAATAAGTGTACCATTCTCTACCCTTAATGACTTACCGGCTGGTTTAATGA
TAAGCGGAGAATTGTATAAAGACGGGGATTTACTTGAAGTCGCTGAATATATTTTAAACATTGTAGGAAAACATTAA

Upstream 100 bases:

>100_bases
AGCTTTTATTACCAAACTAATAGTATTATAACGATGAGGGAAAGCAATAAAGATCCAATCCTTAAAAAGTTACCTGAACC
ATTATTCTGCGTGAGGGAAG

Downstream 100 bases:

>100_bases
CGATCTACTGGGAATGAACCCTAAATTAACTGTCACCTTTTTATTCTTGCTTTTAATGGTAATTATGGGGAATGAGTTGC
AATTAGAAAATAAGATACTT

Product: glutamyl-tRNA amidotransferase, subunit A (gatA-1)

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 398; Mature: 398

Protein sequence:

>398_residues
MKLEELNSKYNAFITLHTIEEGNDKGKLKDVTFGIKDVILTKDVRTTAGSRVLENYVPQRNAYVVDAILREGGKIIGKTN
THEFALGATNTSSIGGPARNPVDPERISGGSSGGSAVAVKLDMVDVGIGTDTGGSVRIPSSLCGVIGFKPTTGLIPNDGV
IPFSWSLDTVGIIVKDNIRLLRRVFDVVLPNEKRKVEIAKLRTRPRLGLFLFDDMEVSRILLKDIYAKLSSHFDIVEVDL
PLLRQYGSKTRRTISLAEAASYHKDWISEYSDKYFKDTYTLLLDGMKISATDYIDALRHRRVLIEEYVKVFKNIDFILSP
TTKIVAPRISDVISNPLQFREYLIANTELFNVVGAPSISVPFSTLNDLPAGLMISGELYKDGDLLEVAEYILNIVGKH

Sequences:

>Translated_398_residues
MKLEELNSKYNAFITLHTIEEGNDKGKLKDVTFGIKDVILTKDVRTTAGSRVLENYVPQRNAYVVDAILREGGKIIGKTN
THEFALGATNTSSIGGPARNPVDPERISGGSSGGSAVAVKLDMVDVGIGTDTGGSVRIPSSLCGVIGFKPTTGLIPNDGV
IPFSWSLDTVGIIVKDNIRLLRRVFDVVLPNEKRKVEIAKLRTRPRLGLFLFDDMEVSRILLKDIYAKLSSHFDIVEVDL
PLLRQYGSKTRRTISLAEAASYHKDWISEYSDKYFKDTYTLLLDGMKISATDYIDALRHRRVLIEEYVKVFKNIDFILSP
TTKIVAPRISDVISNPLQFREYLIANTELFNVVGAPSISVPFSTLNDLPAGLMISGELYKDGDLLEVAEYILNIVGKH
>Mature_398_residues
MKLEELNSKYNAFITLHTIEEGNDKGKLKDVTFGIKDVILTKDVRTTAGSRVLENYVPQRNAYVVDAILREGGKIIGKTN
THEFALGATNTSSIGGPARNPVDPERISGGSSGGSAVAVKLDMVDVGIGTDTGGSVRIPSSLCGVIGFKPTTGLIPNDGV
IPFSWSLDTVGIIVKDNIRLLRRVFDVVLPNEKRKVEIAKLRTRPRLGLFLFDDMEVSRILLKDIYAKLSSHFDIVEVDL
PLLRQYGSKTRRTISLAEAASYHKDWISEYSDKYFKDTYTLLLDGMKISATDYIDALRHRRVLIEEYVKVFKNIDFILSP
TTKIVAPRISDVISNPLQFREYLIANTELFNVVGAPSISVPFSTLNDLPAGLMISGELYKDGDLLEVAEYILNIVGKH

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=482, Percent_Identity=26.5560165975104, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI195972892, Length=413, Percent_Identity=22.0338983050847, Blast_Score=89, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17543272, Length=422, Percent_Identity=28.6729857819905, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17537465, Length=433, Percent_Identity=24.7113163972286, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71990152, Length=136, Percent_Identity=37.5, Blast_Score=82, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6323950, Length=458, Percent_Identity=31.4410480349345, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6319685, Length=414, Percent_Identity=26.5700483091787, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24648113, Length=454, Percent_Identity=31.057268722467, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI24652985, Length=428, Percent_Identity=24.0654205607477, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI19922090, Length=428, Percent_Identity=24.0654205607477, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24652981, Length=428, Percent_Identity=24.0654205607477, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24652983, Length=428, Percent_Identity=24.0654205607477, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24644968, Length=136, Percent_Identity=33.0882352941176, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI45550774, Length=149, Percent_Identity=30.8724832214765, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356731, Length=392, Percent_Identity=23.9795918367347, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648435, Length=149, Percent_Identity=30.8724832214765, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648437, Length=149, Percent_Identity=30.8724832214765, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24648441, Length=149, Percent_Identity=30.8724832214765, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24648439, Length=149, Percent_Identity=30.8724832214765, Blast_Score=73, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 44053; Mature: 44053

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLEELNSKYNAFITLHTIEEGNDKGKLKDVTFGIKDVILTKDVRTTAGSRVLENYVPQR
CCHHHHCCCCEEEEEEEEEECCCCCCCEEEECCCHHHHEEECCHHHHHHHHHHHHHCCCC
NAYVVDAILREGGKIIGKTNTHEFALGATNTSSIGGPARNPVDPERISGGSSGGSAVAVK
CCHHHHHHHHCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCCHHHCCCCCCCCCEEEEE
LDMVDVGIGTDTGGSVRIPSSLCGVIGFKPTTGLIPNDGVIPFSWSLDTVGIIVKDNIRL
EEEEEEECCCCCCCCEECCHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEEEEEECCHHH
LRRVFDVVLPNEKRKVEIAKLRTRPRLGLFLFDDMEVSRILLKDIYAKLSSHFDIVEVDL
HHHHHHHHCCCCCCEEEHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCEEEECH
PLLRQYGSKTRRTISLAEAASYHKDWISEYSDKYFKDTYTLLLDGMKISATDYIDALRHR
HHHHHHCCHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHEEHEECCCEECHHHHHHHHHHH
RVLIEEYVKVFKNIDFILSPTTKIVAPRISDVISNPLQFREYLIANTELFNVVGAPSISV
HHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHEECCCCCCC
PFSTLNDLPAGLMISGELYKDGDLLEVAEYILNIVGKH
CHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLEELNSKYNAFITLHTIEEGNDKGKLKDVTFGIKDVILTKDVRTTAGSRVLENYVPQR
CCHHHHCCCCEEEEEEEEEECCCCCCCEEEECCCHHHHEEECCHHHHHHHHHHHHHCCCC
NAYVVDAILREGGKIIGKTNTHEFALGATNTSSIGGPARNPVDPERISGGSSGGSAVAVK
CCHHHHHHHHCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCCHHHCCCCCCCCCEEEEE
LDMVDVGIGTDTGGSVRIPSSLCGVIGFKPTTGLIPNDGVIPFSWSLDTVGIIVKDNIRL
EEEEEEECCCCCCCCEECCHHHHHHHCCCCCCCCCCCCCEEEEEECCCEEEEEEECCHHH
LRRVFDVVLPNEKRKVEIAKLRTRPRLGLFLFDDMEVSRILLKDIYAKLSSHFDIVEVDL
HHHHHHHHCCCCCCEEEHHHHCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCEEEECH
PLLRQYGSKTRRTISLAEAASYHKDWISEYSDKYFKDTYTLLLDGMKISATDYIDALRHR
HHHHHHCCHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHEEHEECCCEECHHHHHHHHHHH
RVLIEEYVKVFKNIDFILSPTTKIVAPRISDVISNPLQFREYLIANTELFNVVGAPSISV
HHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHEECCCCCCC
PFSTLNDLPAGLMISGELYKDGDLLEVAEYILNIVGKH
CHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA