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The map label for this gene is carA

Identifier: 15897553

GI number: 15897553

Start: 554869

End: 555972

Strand: Direct

Name: carA

Synonym: SSO0640

Alternate gene names: 15897553

Gene position: 554869-555972 (Clockwise)

Preceding gene: 15897552

Following gene: 15897554

Centisome position: 18.54

GC content: 34.42

Gene sequence:

>1104_bases
ATGAAATTGGAGAATAAGAAGGGTTATTTATATCTAGAGGACGGAACGTTTATTGAAGGATATAGTTTTGGAGCTAAAGG
AATCAAGGTAGGAGAGGTAGTGTTTACAACTTCTATGAATGGATACGTGGAGAGTTTAACAGATCCTTCATATAAGGGTC
AAATACTAATTATTACGCATCCATTAGTTGGAAATTATGGTGTACCCGAAAAGAAGTATGAACAAGGTATTCTAACCAAC
TTCGAGTCAGAAAGAATACAAGTTGAAGGCTTAATAGTAGCTGAGCATACATATCCTAGTAAATGGAACTCAGCGCTGAC
ATTAGATGAGTGGCTGAAATCAGAGAACGTACCAGGAGTTTTTGATGTCGATACGAGAATGATTGTAAAAAAGATTAGAA
CCTATGGCACGATGATGGGAATTATAGCTTCTGAATTAGAAATAGACGATCCTAGAAAATATTTAGAAAAAAAGTACGAT
GAAATAGATTTCACACAATTTACATCACCCAAGTCACCAATCTTTCATCCAAATACTGGAGATATGATCGTAGTAGTGGA
TTGTGGAATAAAACATGGAATTTTGTACGGACTTTATAAGAGAGGATTCTCAATTGTTAGAGTCCCTTGCAGTTTTAGTG
CTAGTAAGATAATTGAGTATAATCCTAAGGGAATTGTATTTAGTAATGGTCCAGGTAATCCCAATTTGCTAGAAAATCAA
ATCAAGACGTTCTCTGAATTAGTGGAGTATAAGATTCCAATCTTAGGTATTTGCTTAGGGCATCAAATAGCAACTCTAGC
ACTGGGAGGTAAAATTAAGAAAATGAAATTCGGACATAGGGCAATAAACAAACCAGTAATTGAAAGCAATTCAAATAAAT
GCTATATATCCACTCATAATCATGGATATGGTATAATATCTAAAAACGATATCCCGCCTAATACTAAAATTTGGTTCTAT
AATCCAGACGATTATACAATAGAAGGCTTAATCCATGAAAAATTACCAATTATCACTACACAATTTCATCCTGAAGCTAG
ACCAGGTCCTTGGGATACAACTTGGGTATTTGATAAATTTAGAACTATGGTGACTGGAAAATGA

Upstream 100 bases:

>100_bases
ATAAAAATTAGAGAAACTAAATTGAAAGAAGATGAAAAGGAAATCGAGGAGTATAAGTTAAAAATAATCTCCAAATTAGG
AGAATTACAAGTGATCGAAA

Downstream 100 bases:

>100_bases
GAGAAACTCCAAAAAAGGTTCTGGTTATTGGATCTGGACCTATAAAAATAGCTGAAGCTGCAGAGTTTGATTACAGTGGA
AGTCAAGCGTTAAAGGCATT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MKLENKKGYLYLEDGTFIEGYSFGAKGIKVGEVVFTTSMNGYVESLTDPSYKGQILIITHPLVGNYGVPEKKYEQGILTN
FESERIQVEGLIVAEHTYPSKWNSALTLDEWLKSENVPGVFDVDTRMIVKKIRTYGTMMGIIASELEIDDPRKYLEKKYD
EIDFTQFTSPKSPIFHPNTGDMIVVVDCGIKHGILYGLYKRGFSIVRVPCSFSASKIIEYNPKGIVFSNGPGNPNLLENQ
IKTFSELVEYKIPILGICLGHQIATLALGGKIKKMKFGHRAINKPVIESNSNKCYISTHNHGYGIISKNDIPPNTKIWFY
NPDDYTIEGLIHEKLPIITTQFHPEARPGPWDTTWVFDKFRTMVTGK

Sequences:

>Translated_367_residues
MKLENKKGYLYLEDGTFIEGYSFGAKGIKVGEVVFTTSMNGYVESLTDPSYKGQILIITHPLVGNYGVPEKKYEQGILTN
FESERIQVEGLIVAEHTYPSKWNSALTLDEWLKSENVPGVFDVDTRMIVKKIRTYGTMMGIIASELEIDDPRKYLEKKYD
EIDFTQFTSPKSPIFHPNTGDMIVVVDCGIKHGILYGLYKRGFSIVRVPCSFSASKIIEYNPKGIVFSNGPGNPNLLENQ
IKTFSELVEYKIPILGICLGHQIATLALGGKIKKMKFGHRAINKPVIESNSNKCYISTHNHGYGIISKNDIPPNTKIWFY
NPDDYTIEGLIHEKLPIITTQFHPEARPGPWDTTWVFDKFRTMVTGK
>Mature_367_residues
MKLENKKGYLYLEDGTFIEGYSFGAKGIKVGEVVFTTSMNGYVESLTDPSYKGQILIITHPLVGNYGVPEKKYEQGILTN
FESERIQVEGLIVAEHTYPSKWNSALTLDEWLKSENVPGVFDVDTRMIVKKIRTYGTMMGIIASELEIDDPRKYLEKKYD
EIDFTQFTSPKSPIFHPNTGDMIVVVDCGIKHGILYGLYKRGFSIVRVPCSFSASKIIEYNPKGIVFSNGPGNPNLLENQ
IKTFSELVEYKIPILGICLGHQIATLALGGKIKKMKFGHRAINKPVIESNSNKCYISTHNHGYGIISKNDIPPNTKIWFY
NPDDYTIEGLIHEKLPIITTQFHPEARPGPWDTTWVFDKFRTMVTGK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI18105007, Length=366, Percent_Identity=37.7049180327869, Blast_Score=253, Evalue=3e-67,
Organism=Homo sapiens, GI21361331, Length=369, Percent_Identity=37.1273712737127, Blast_Score=247, Evalue=1e-65,
Organism=Homo sapiens, GI169790915, Length=369, Percent_Identity=37.1273712737127, Blast_Score=247, Evalue=2e-65,
Organism=Escherichia coli, GI1786215, Length=385, Percent_Identity=34.2857142857143, Blast_Score=214, Evalue=7e-57,
Organism=Caenorhabditis elegans, GI193204318, Length=367, Percent_Identity=38.4196185286104, Blast_Score=257, Evalue=7e-69,
Organism=Saccharomyces cerevisiae, GI6324878, Length=373, Percent_Identity=40.4825737265416, Blast_Score=263, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6322331, Length=397, Percent_Identity=36.0201511335013, Blast_Score=247, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24642586, Length=375, Percent_Identity=37.0666666666667, Blast_Score=238, Evalue=4e-63,
Organism=Drosophila melanogaster, GI45555749, Length=375, Percent_Identity=37.0666666666667, Blast_Score=238, Evalue=5e-63,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): CARA_SULSO (Q59968)

Other databases:

- EMBL:   U33768
- EMBL:   Y18930
- EMBL:   AE006641
- PIR:   T43252
- RefSeq:   NP_342158.1
- ProteinModelPortal:   Q59968
- SMR:   Q59968
- GeneID:   1454915
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0640
- NMPDR:   fig|273057.1.peg.581
- HOGENOM:   HBG286341
- OMA:   DTEKLRF
- PhylomeDB:   Q59968
- ProtClustDB:   PRK12564
- BioCyc:   SSOL273057:SSO0640-MONOMER
- BRENDA:   6.3.5.5
- HAMAP:   MF_01209_A
- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991
- PANTHER:   PTHR11405:SF4
- PRINTS:   PR00097
- PRINTS:   PR00099
- PRINTS:   PR00096
- TIGRFAMs:   TIGR01368

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase; SSF52021 CP_synthsmall

EC number: =6.3.5.5

Molecular weight: Translated: 41480; Mature: 41480

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 258-258 ACT_SITE 343-343 ACT_SITE 345-345

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLENKKGYLYLEDGTFIEGYSFGAKGIKVGEVVFTTSMNGYVESLTDPSYKGQILIITH
CCCCCCCCEEEEECCCEEECCCCCCCCEEEEEEEEEECCCCHHHHCCCCCCCCEEEEEEC
PLVGNYGVPEKKYEQGILTNFESERIQVEGLIVAEHTYPSKWNSALTLDEWLKSENVPGV
CCCCCCCCCHHHHHCHHHCCCCCCEEEEEEEEEEECCCCCCCCCCEEHHHHHHCCCCCCE
FDVDTRMIVKKIRTYGTMMGIIASELEIDDPRKYLEKKYDEIDFTQFTSPKSPIFHPNTG
EECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEECCCCCCCEECCCCC
DMIVVVDCGIKHGILYGLYKRGFSIVRVPCSFSASKIIEYNPKGIVFSNGPGNPNLLENQ
CEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCEEEECCCCCCCCHHHHH
IKTFSELVEYKIPILGICLGHQIATLALGGKIKKMKFGHRAINKPVIESNSNKCYISTHN
HHHHHHHHHHCCCEEEEECCCCEEEEEECCEEEEEECCHHHHCCCEEECCCCEEEEEECC
HGYGIISKNDIPPNTKIWFYNPDDYTIEGLIHEKLPIITTQFHPEARPGPWDTTWVFDKF
CCEEEEECCCCCCCCEEEEECCCCEEEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHH
RTMVTGK
HHHHCCC
>Mature Secondary Structure
MKLENKKGYLYLEDGTFIEGYSFGAKGIKVGEVVFTTSMNGYVESLTDPSYKGQILIITH
CCCCCCCCEEEEECCCEEECCCCCCCCEEEEEEEEEECCCCHHHHCCCCCCCCEEEEEEC
PLVGNYGVPEKKYEQGILTNFESERIQVEGLIVAEHTYPSKWNSALTLDEWLKSENVPGV
CCCCCCCCCHHHHHCHHHCCCCCCEEEEEEEEEEECCCCCCCCCCEEHHHHHHCCCCCCE
FDVDTRMIVKKIRTYGTMMGIIASELEIDDPRKYLEKKYDEIDFTQFTSPKSPIFHPNTG
EECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCEECCCCCCCEECCCCC
DMIVVVDCGIKHGILYGLYKRGFSIVRVPCSFSASKIIEYNPKGIVFSNGPGNPNLLENQ
CEEEEEECCCCHHHHHHHHHCCCEEEEEECCCCCCEEEEECCCEEEECCCCCCCCHHHHH
IKTFSELVEYKIPILGICLGHQIATLALGGKIKKMKFGHRAINKPVIESNSNKCYISTHN
HHHHHHHHHHCCCEEEEECCCCEEEEEECCEEEEEECCHHHHCCCEEECCCCEEEEEECC
HGYGIISKNDIPPNTKIWFYNPDDYTIEGLIHEKLPIITTQFHPEARPGPWDTTWVFDKF
CCEEEEECCCCCCCCEEEEECCCCEEEEEEEECCCCEEEEECCCCCCCCCCCCHHHHHHH
RTMVTGK
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8752005; 10701121; 11427726