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The map label for this gene is purF-1 [H]

Identifier: 15897546

GI number: 15897546

Start: 546572

End: 547924

Strand: Direct

Name: purF-1 [H]

Synonym: SSO0632

Alternate gene names: 15897546

Gene position: 546572-547924 (Clockwise)

Preceding gene: 15897545

Following gene: 15897547

Centisome position: 18.27

GC content: 35.55

Gene sequence:

>1353_bases
ATGGATAGCATACGTGATAAATGTGGAGTCTTTGCTGTATCTAGCCCTAAAGAAGTAAATATTCAACTGGTAGTAGAGGG
AATTAGACTACTTCAGCATAGGGGTCAAGAATCTGCAGGAATCGCATATGCTGAGAATGGGGAAATATTGACGATTAAGG
GATCTGGACTTGTAGACGAAGTATTTAAGGAAAATCTTAATAAATTTATAAAAAATGGAATAGGTCATGTAAGATATTCA
ACAAGTGGAAAAAGCTCAATTGAAGAAGCACAGCCTTTAGGCGACTCAAAAATAGTCGTGGCGTTCAATGGGACAATAAG
CAACTATTACCAGTTTGGATCATTTAGGGTAGATACTGAATTTATATATAAGTTCTTTAAGCAAAAATTGGCCTTTCGCT
CTATCCCTGATACTGTAAAGGAATTTATGAAAGTTGTCGATGGTGGATACTCTGTAGCTATTCTTTTGAATGATGAAGAG
ATTGTAGTATTTAGAGATCCTAAAGGATTTCATCCAGTTGTATTAGGATTTTTAGAAGGATCATTAATAGTTTCCTCGGA
GGATTCCGTTATTAGACAACTAGGTGGGAGCGTTTTGAAACATATTCTACCAAGCGAAATGATAATTATGAAAAATGGCA
AAATATTATATGACAAAGTAATCTATGAGGAGAAAAATTACGCTACTTGTTCCTTCGAATATATATACTTTGCAAGACCA
GATTCCAATATAGATGGGCATTCAGTTTACTTAGCTAGGATAAGGTTAGGTGAATTATTAGCAGAAAAACACCCAGCTAA
CGGAGATATTGTAGTTCCAGTGCCGGACTCATCAAGGCCAATAGCGCTGGGCTTTTCTAGAAAAAGTCACATACCTTTAG
AGGAACCTCTAGTTAGAACAATTTCCTCTGTAAGGTCCTTCATAATGCCAACTCAAGATAAGAGAAATGAGGTATTAGAA
GAGAAATTTGGAGTGGTTGCAGATGCAGTAAGGGGGAAAAGAATAGTTTTAATAGACGACTCAATAGTTAGGGGAAATAC
TATGAAGAGAATAATAACTATGTTACGTAAAGCCGGCGCCAAAGAAATTCATGTACGTATAGGATCACCTATGATAAAAT
ACCCTTGTTATATGGGAATAGACTTTCCTAAAAGAGAAGAGTTGATTGCGCATAATAAATCTGAAAAAGAAATAGGAAAT
GAGCTGAACGCTGATTCTATTGAATTTCTAAGTGTCGAAGAGATGTTACAAGGAATAGGTAGAAGAGACTTATGCAATGC
ATGCTTTTCTGGGATTTATCCTCTCAAATTTAACTATAATTCTACAGAGTTAGAAAGAATATTTGGGAAGTGA

Upstream 100 bases:

>100_bases
GTCAATAAAAAAACTAATATTTTAACTATTGATAATATAGATTATAATCTTAAAAATATAGTTGATAATTACTTTAATTT
TCTAGAAGAGGTAATGGGTA

Downstream 100 bases:

>100_bases
TATTAATGGCTGGAATACTAGGTATATTAGCGTTCGATGATGTATGGAATGTATCTAGATTTTTATATTATGGTCTAATA
GGCTTGCAACATAGAGGATA

Product: glutamine phosphoribosylpyrophosphate amidotransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MDSIRDKCGVFAVSSPKEVNIQLVVEGIRLLQHRGQESAGIAYAENGEILTIKGSGLVDEVFKENLNKFIKNGIGHVRYS
TSGKSSIEEAQPLGDSKIVVAFNGTISNYYQFGSFRVDTEFIYKFFKQKLAFRSIPDTVKEFMKVVDGGYSVAILLNDEE
IVVFRDPKGFHPVVLGFLEGSLIVSSEDSVIRQLGGSVLKHILPSEMIIMKNGKILYDKVIYEEKNYATCSFEYIYFARP
DSNIDGHSVYLARIRLGELLAEKHPANGDIVVPVPDSSRPIALGFSRKSHIPLEEPLVRTISSVRSFIMPTQDKRNEVLE
EKFGVVADAVRGKRIVLIDDSIVRGNTMKRIITMLRKAGAKEIHVRIGSPMIKYPCYMGIDFPKREELIAHNKSEKEIGN
ELNADSIEFLSVEEMLQGIGRRDLCNACFSGIYPLKFNYNSTELERIFGK

Sequences:

>Translated_450_residues
MDSIRDKCGVFAVSSPKEVNIQLVVEGIRLLQHRGQESAGIAYAENGEILTIKGSGLVDEVFKENLNKFIKNGIGHVRYS
TSGKSSIEEAQPLGDSKIVVAFNGTISNYYQFGSFRVDTEFIYKFFKQKLAFRSIPDTVKEFMKVVDGGYSVAILLNDEE
IVVFRDPKGFHPVVLGFLEGSLIVSSEDSVIRQLGGSVLKHILPSEMIIMKNGKILYDKVIYEEKNYATCSFEYIYFARP
DSNIDGHSVYLARIRLGELLAEKHPANGDIVVPVPDSSRPIALGFSRKSHIPLEEPLVRTISSVRSFIMPTQDKRNEVLE
EKFGVVADAVRGKRIVLIDDSIVRGNTMKRIITMLRKAGAKEIHVRIGSPMIKYPCYMGIDFPKREELIAHNKSEKEIGN
ELNADSIEFLSVEEMLQGIGRRDLCNACFSGIYPLKFNYNSTELERIFGK
>Mature_450_residues
MDSIRDKCGVFAVSSPKEVNIQLVVEGIRLLQHRGQESAGIAYAENGEILTIKGSGLVDEVFKENLNKFIKNGIGHVRYS
TSGKSSIEEAQPLGDSKIVVAFNGTISNYYQFGSFRVDTEFIYKFFKQKLAFRSIPDTVKEFMKVVDGGYSVAILLNDEE
IVVFRDPKGFHPVVLGFLEGSLIVSSEDSVIRQLGGSVLKHILPSEMIIMKNGKILYDKVIYEEKNYATCSFEYIYFARP
DSNIDGHSVYLARIRLGELLAEKHPANGDIVVPVPDSSRPIALGFSRKSHIPLEEPLVRTISSVRSFIMPTQDKRNEVLE
EKFGVVADAVRGKRIVLIDDSIVRGNTMKRIITMLRKAGAKEIHVRIGSPMIKYPCYMGIDFPKREELIAHNKSEKEIGN
ELNADSIEFLSVEEMLQGIGRRDLCNACFSGIYPLKFNYNSTELERIFGK

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=516, Percent_Identity=36.4341085271318, Blast_Score=276, Evalue=4e-74,
Organism=Escherichia coli, GI1788651, Length=447, Percent_Identity=32.8859060402685, Blast_Score=230, Evalue=2e-61,
Organism=Caenorhabditis elegans, GI17554892, Length=485, Percent_Identity=34.4329896907216, Blast_Score=240, Evalue=9e-64,
Organism=Saccharomyces cerevisiae, GI6323958, Length=450, Percent_Identity=29.7777777777778, Blast_Score=199, Evalue=6e-52,
Organism=Drosophila melanogaster, GI24659598, Length=490, Percent_Identity=35.1020408163265, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI28573187, Length=492, Percent_Identity=34.349593495935, Blast_Score=259, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24659604, Length=469, Percent_Identity=34.9680170575693, Blast_Score=256, Evalue=2e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 50495; Mature: 50495

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSIRDKCGVFAVSSPKEVNIQLVVEGIRLLQHRGQESAGIAYAENGEILTIKGSGLVDE
CCCCHHHCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCCCHHH
VFKENLNKFIKNGIGHVRYSTSGKSSIEEAQPLGDSKIVVAFNGTISNYYQFGSFRVDTE
HHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCCCEEEEEECCCHHHHEECCCEEECHH
FIYKFFKQKLAFRSIPDTVKEFMKVVDGGYSVAILLNDEEIVVFRDPKGFHPVVLGFLEG
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEECCCEEEEEECCCCCCHHEEEEECC
SLIVSSEDSVIRQLGGSVLKHILPSEMIIMKNGKILYDKVIYEEKNYATCSFEYIYFARP
EEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEECCCCEEEEEEEEEEECC
DSNIDGHSVYLARIRLGELLAEKHPANGDIVVPVPDSSRPIALGFSRKSHIPLEEPLVRT
CCCCCCCEEEEEHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEECCCCCCCCCHHHHHHH
ISSVRSFIMPTQDKRNEVLEEKFGVVADAVRGKRIVLIDDSIVRGNTMKRIITMLRKAGA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCHHHHHHHHHHHCCC
KEIHVRIGSPMIKYPCYMGIDFPKREELIAHNKSEKEIGNELNADSIEFLSVEEMLQGIG
CEEEEEECCCEEECCEEECCCCCCHHHHHHCCCCHHHHCCCCCCCCCCEEEHHHHHHHCC
RRDLCNACFSGIYPLKFNYNSTELERIFGK
CHHHHHHHHCCCCEEEECCCHHHHHHHHCC
>Mature Secondary Structure
MDSIRDKCGVFAVSSPKEVNIQLVVEGIRLLQHRGQESAGIAYAENGEILTIKGSGLVDE
CCCCHHHCCEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCEEEEECCCCHHH
VFKENLNKFIKNGIGHVRYSTSGKSSIEEAQPLGDSKIVVAFNGTISNYYQFGSFRVDTE
HHHHHHHHHHHCCCCEEEEECCCCCHHHHHCCCCCCEEEEEECCCHHHHEECCCEEECHH
FIYKFFKQKLAFRSIPDTVKEFMKVVDGGYSVAILLNDEEIVVFRDPKGFHPVVLGFLEG
HHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEECCCEEEEEECCCCCCHHEEEEECC
SLIVSSEDSVIRQLGGSVLKHILPSEMIIMKNGKILYDKVIYEEKNYATCSFEYIYFARP
EEEEECCHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEEEECCCCEEEEEEEEEEECC
DSNIDGHSVYLARIRLGELLAEKHPANGDIVVPVPDSSRPIALGFSRKSHIPLEEPLVRT
CCCCCCCEEEEEHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEECCCCCCCCCHHHHHHH
ISSVRSFIMPTQDKRNEVLEEKFGVVADAVRGKRIVLIDDSIVRGNTMKRIITMLRKAGA
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEECCCEECCCHHHHHHHHHHHCCC
KEIHVRIGSPMIKYPCYMGIDFPKREELIAHNKSEKEIGNELNADSIEFLSVEEMLQGIG
CEEEEEECCCEEECCEEECCCCCCHHHHHHCCCCHHHHCCCCCCCCCCEEEHHHHHHHCC
RRDLCNACFSGIYPLKFNYNSTELERIFGK
CHHHHHHHHCCCCEEEECCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]