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The map label for this gene is purF-2 [H]

Identifier: 15897547

GI number: 15897547

Start: 547921

End: 549135

Strand: Direct

Name: purF-2 [H]

Synonym: SSO0633

Alternate gene names: 15897547

Gene position: 547921-549135 (Clockwise)

Preceding gene: 15897546

Following gene: 15897548

Centisome position: 18.31

GC content: 37.2

Gene sequence:

>1215_bases
GTGATATTAATGGCTGGAATACTAGGTATATTAGCGTTCGATGATGTATGGAATGTATCTAGATTTTTATATTATGGTCT
AATAGGCTTGCAACATAGAGGATATTCTAAAAGTGGAATAGCAATATTGAACAAGCAGAGGAATATATTTACTAAACTAG
AGAACGTAGCGCCTGAGGATTTAGAAATTAAAGACTTGAATGGATGGGCTGGAATAGGTTATGCTGGAATCAGAAATAGT
TATCCCATAACAATAGATAATGGTGCTATAGTCGTTGATGGGATGATAGATGATAACAATGTTCTTAACCATGTCATAAG
AGAGCCAGAAAATGCAATAGATGATATTAAAAAGCCGATCTCGTTCATAGCCATTAGTAAAGACGGCCAGTTAATAGCCT
ATAGAGACGAACTAGGACTTAAGCCGTTAAGCATAGGTGGATTTGGATTCGATTTAGCAGTTATTTCCTCCGAACCAACG
TCAATGTTTGTAATAGGGGCTGAGTTCAAAAGGGAAATTAATCCGGGAGAGTTAGTGATCGTTGACAAATATCATATTGA
ATCCAAACAGGTGAGAATACCTAGAAAATCGTATTGTACTATAGAGTACGTATACCAAGCTAGAATAGACAGTATGATCA
ACGAAAGAGAAATCTATGACCTAAGAGTGAAAATAGGGGAAGAATTAGCCTTAGAATATCCAATAAATGCTGATTCAGTA
ATAGGAGTTCCAGAAACCGCATTACCTTTCGCAATAGGCTATTCCAAGAAATTAAATATACCATTAGACTTAGGCTTTAC
GAGGACAGGGAGCCCAATAAGGACAATGTTATCTTCTGATGCTTTCCTAAAAATAGTTGGGGTCCAACTTAAGCTAAACC
CAATAAAAAGTGCAGTAAAAGGAAAGAGAATAATTCTAATAGATGATTCTATGGTCACCGGAACTACCCTTAAAAACACT
ATATTTAACTTGAGAAAATTGGGCGCAAAGGAAATCCACGTGCTGATAGGAAGCCCTAAATTAGTCTCACAATGTCCGTA
CGGAGTTGAAGTCCCCGACGAAAAAGAATTGATCTCAGCTAATCTTAACGACGAAACTATAGCGAGGGTTTTAGGTGCAG
ACTCTATACACTGGTTAAGCATAGAAGGATTGTTCAAAGCCATTGGGCATAGGGATCTCTGCTTAGGATGTATGACTAAA
AAATATCCATGGTGA

Upstream 100 bases:

>100_bases
AAGGAATAGGTAGAAGAGACTTATGCAATGCATGCTTTTCTGGGATTTATCCTCTCAAATTTAACTATAATTCTACAGAG
TTAGAAAGAATATTTGGGAA

Downstream 100 bases:

>100_bases
GAAAAATGAAAGTATTACTCGTTGGAGATGGAGCTAGAGAAAACGTTCTAGCCTATTCGTTGGCAAGATCATCTAAAGGT
TACAAGATTTACGCACTATC

Product: glutamine phosphoribosylpyrophosphate amidotransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MILMAGILGILAFDDVWNVSRFLYYGLIGLQHRGYSKSGIAILNKQRNIFTKLENVAPEDLEIKDLNGWAGIGYAGIRNS
YPITIDNGAIVVDGMIDDNNVLNHVIREPENAIDDIKKPISFIAISKDGQLIAYRDELGLKPLSIGGFGFDLAVISSEPT
SMFVIGAEFKREINPGELVIVDKYHIESKQVRIPRKSYCTIEYVYQARIDSMINEREIYDLRVKIGEELALEYPINADSV
IGVPETALPFAIGYSKKLNIPLDLGFTRTGSPIRTMLSSDAFLKIVGVQLKLNPIKSAVKGKRIILIDDSMVTGTTLKNT
IFNLRKLGAKEIHVLIGSPKLVSQCPYGVEVPDEKELISANLNDETIARVLGADSIHWLSIEGLFKAIGHRDLCLGCMTK
KYPW

Sequences:

>Translated_404_residues
MILMAGILGILAFDDVWNVSRFLYYGLIGLQHRGYSKSGIAILNKQRNIFTKLENVAPEDLEIKDLNGWAGIGYAGIRNS
YPITIDNGAIVVDGMIDDNNVLNHVIREPENAIDDIKKPISFIAISKDGQLIAYRDELGLKPLSIGGFGFDLAVISSEPT
SMFVIGAEFKREINPGELVIVDKYHIESKQVRIPRKSYCTIEYVYQARIDSMINEREIYDLRVKIGEELALEYPINADSV
IGVPETALPFAIGYSKKLNIPLDLGFTRTGSPIRTMLSSDAFLKIVGVQLKLNPIKSAVKGKRIILIDDSMVTGTTLKNT
IFNLRKLGAKEIHVLIGSPKLVSQCPYGVEVPDEKELISANLNDETIARVLGADSIHWLSIEGLFKAIGHRDLCLGCMTK
KYPW
>Mature_404_residues
MILMAGILGILAFDDVWNVSRFLYYGLIGLQHRGYSKSGIAILNKQRNIFTKLENVAPEDLEIKDLNGWAGIGYAGIRNS
YPITIDNGAIVVDGMIDDNNVLNHVIREPENAIDDIKKPISFIAISKDGQLIAYRDELGLKPLSIGGFGFDLAVISSEPT
SMFVIGAEFKREINPGELVIVDKYHIESKQVRIPRKSYCTIEYVYQARIDSMINEREIYDLRVKIGEELALEYPINADSV
IGVPETALPFAIGYSKKLNIPLDLGFTRTGSPIRTMLSSDAFLKIVGVQLKLNPIKSAVKGKRIILIDDSMVTGTTLKNT
IFNLRKLGAKEIHVLIGSPKLVSQCPYGVEVPDEKELISANLNDETIARVLGADSIHWLSIEGLFKAIGHRDLCLGCMTK
KYPW

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=506, Percent_Identity=27.4703557312253, Blast_Score=174, Evalue=2e-43,
Organism=Escherichia coli, GI1788651, Length=455, Percent_Identity=27.2527472527473, Blast_Score=132, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17554892, Length=320, Percent_Identity=31.5625, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6323958, Length=450, Percent_Identity=27.5555555555556, Blast_Score=130, Evalue=3e-31,
Organism=Drosophila melanogaster, GI28573187, Length=485, Percent_Identity=29.8969072164948, Blast_Score=192, Evalue=5e-49,
Organism=Drosophila melanogaster, GI24659598, Length=486, Percent_Identity=29.0123456790123, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24659604, Length=458, Percent_Identity=29.9126637554585, Blast_Score=179, Evalue=4e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 44840; Mature: 44840

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILMAGILGILAFDDVWNVSRFLYYGLIGLQHRGYSKSGIAILNKQRNIFTKLENVAPED
CEEHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEECCCHHHHHHHHCCCCC
LEIKDLNGWAGIGYAGIRNSYPITIDNGAIVVDGMIDDNNVLNHVIREPENAIDDIKKPI
CEEEECCCCCCCCCCCCCCCCCEEECCCEEEEEEEECCCHHHHHHHHCCHHHHHHHHCCE
SFIAISKDGQLIAYRDELGLKPLSIGGFGFDLAVISSEPTSMFVIGAEFKREINPGELVI
EEEEECCCCCEEEEECCCCCCEEEECCCCEEEEEEECCCCEEEEEEHHHHCCCCCCCEEE
VDKYHIESKQVRIPRKSYCTIEYVYQARIDSMINEREIYDLRVKIGEELALEYPINADSV
EEEEEECCCEEECCCCCCEEEEEHHHHHHHHHHCCCEEEEEEEEECCEEEEECCCCCCCE
IGVPETALPFAIGYSKKLNIPLDLGFTRTGSPIRTMLSSDAFLKIVGVQLKLNPIKSAVK
ECCCCCCCCEEECCCCCCCCEEECCCCCCCCHHHHHHCCCCEEEEEEEEEEECHHHHHHC
GKRIILIDDSMVTGTTLKNTIFNLRKLGAKEIHVLIGSPKLVSQCPYGVEVPDEKELISA
CCEEEEEECCEECCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHCCCCCCCCCCHHHEEC
NLNDETIARVLGADSIHWLSIEGLFKAIGHRDLCLGCMTKKYPW
CCCHHHHHHHHCCCCEEEEEHHHHHHHCCCCCEEEHHHCCCCCC
>Mature Secondary Structure
MILMAGILGILAFDDVWNVSRFLYYGLIGLQHRGYSKSGIAILNKQRNIFTKLENVAPED
CEEHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEECCCHHHHHHHHCCCCC
LEIKDLNGWAGIGYAGIRNSYPITIDNGAIVVDGMIDDNNVLNHVIREPENAIDDIKKPI
CEEEECCCCCCCCCCCCCCCCCEEECCCEEEEEEEECCCHHHHHHHHCCHHHHHHHHCCE
SFIAISKDGQLIAYRDELGLKPLSIGGFGFDLAVISSEPTSMFVIGAEFKREINPGELVI
EEEEECCCCCEEEEECCCCCCEEEECCCCEEEEEEECCCCEEEEEEHHHHCCCCCCCEEE
VDKYHIESKQVRIPRKSYCTIEYVYQARIDSMINEREIYDLRVKIGEELALEYPINADSV
EEEEEECCCEEECCCCCCEEEEEHHHHHHHHHHCCCEEEEEEEEECCEEEEECCCCCCCE
IGVPETALPFAIGYSKKLNIPLDLGFTRTGSPIRTMLSSDAFLKIVGVQLKLNPIKSAVK
ECCCCCCCCEEECCCCCCCCEEECCCCCCCCHHHHHHCCCCEEEEEEEEEEECHHHHHHC
GKRIILIDDSMVTGTTLKNTIFNLRKLGAKEIHVLIGSPKLVSQCPYGVEVPDEKELISA
CCEEEEEECCEECCCHHHHHHHHHHHCCCCEEEEEECCCHHHHHCCCCCCCCCCHHHEEC
NLNDETIARVLGADSIHWLSIEGLFKAIGHRDLCLGCMTKKYPW
CCCHHHHHHHHCCCCEEEEEHHHHHHHCCCCCEEEHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]