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The map label for this gene is metS-like [H]

Identifier: 15897541

GI number: 15897541

Start: 542368

End: 542856

Strand: Reverse

Name: metS-like [H]

Synonym: SSO0627

Alternate gene names: 15897541

Gene position: 542856-542368 (Counterclockwise)

Preceding gene: 15897556

Following gene: 15897540

Centisome position: 18.14

GC content: 33.95

Gene sequence:

>489_bases
TTGGTCTTTCCCTCAGAAATCTTGTTAACTTCCATTACGACAATTTTACATAACCGATATAAAAAGTTAGCTTCAAATAA
GTATAAACTTTTACTTCATTATGTAAATCAAAATCCTTATAATTCTTCACCAATGCTTAACATCATGAGTAATCAAATTA
CAATAGACGATTTTGCAAAATTAGACCTTAAGGTTGGAATTGTGAGACAAGCTGAAAGGATTGAAGGAACAAGATTACTA
AAACTATTGATTGACTTGGGTTCAGAACAAAGGCAAATAATTGCGGGTTTAGGAGAATATTATACCCCAGAGGAGTTATT
GAACAAGAGGATAATAGTTATAGCCAATTTAAAGCCTAGAGTGATAAGAGGTTTTGAGAGCCAAGGTATGTTATTGGCCG
CTGGATGTAAAGAGGATGAAGCGAAAGGAATAAAACCCAGAATACTAACTGTAGACGGAGAAGTTCCTCCAGGAACTAAG
ATATGTTAA

Upstream 100 bases:

>100_bases
GCCCTTACCCTCCAATATATCTCTTTTTTGACCATCACCCGCGGTTATGTCATCCTTAAACTCGAGTAATACGTGATCTT
TATCAAAATCATAAACTATC

Downstream 100 bases:

>100_bases
ATCCATTTGAGAAGTTACAGATCCCGCCAAAAGCCAATCAAACTATTGATACCATGCTAAAAAGGCTGCCAAAGATAGGT
GGTACTACAGTTAGGGATAG

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MVFPSEILLTSITTILHNRYKKLASNKYKLLLHYVNQNPYNSSPMLNIMSNQITIDDFAKLDLKVGIVRQAERIEGTRLL
KLLIDLGSEQRQIIAGLGEYYTPEELLNKRIIVIANLKPRVIRGFESQGMLLAAGCKEDEAKGIKPRILTVDGEVPPGTK
IC

Sequences:

>Translated_162_residues
MVFPSEILLTSITTILHNRYKKLASNKYKLLLHYVNQNPYNSSPMLNIMSNQITIDDFAKLDLKVGIVRQAERIEGTRLL
KLLIDLGSEQRQIIAGLGEYYTPEELLNKRIIVIANLKPRVIRGFESQGMLLAAGCKEDEAKGIKPRILTVDGEVPPGTK
IC
>Mature_162_residues
MVFPSEILLTSITTILHNRYKKLASNKYKLLLHYVNQNPYNSSPMLNIMSNQITIDDFAKLDLKVGIVRQAERIEGTRLL
KLLIDLGSEQRQIIAGLGEYYTPEELLNKRIIVIANLKPRVIRGFESQGMLLAAGCKEDEAKGIKPRILTVDGEVPPGTK
IC

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0073

COG function: function code R; EMAP domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788432, Length=115, Percent_Identity=38.2608695652174, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1789455, Length=106, Percent_Identity=32.0754716981132, Blast_Score=60, Evalue=7e-11,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 18221; Mature: 18221

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFPSEILLTSITTILHNRYKKLASNKYKLLLHYVNQNPYNSSPMLNIMSNQITIDDFAK
CCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCEEEECCCEEEHHHHHH
LDLKVGIVRQAERIEGTRLLKLLIDLGSEQRQIIAGLGEYYTPEELLNKRIIVIANLKPR
HHEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHCCCEEEEEECCCHH
VIRGFESQGMLLAAGCKEDEAKGIKPRILTVDGEVPPGTKIC
HHCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MVFPSEILLTSITTILHNRYKKLASNKYKLLLHYVNQNPYNSSPMLNIMSNQITIDDFAK
CCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCEEEECCCEEEHHHHHH
LDLKVGIVRQAERIEGTRLLKLLIDLGSEQRQIIAGLGEYYTPEELLNKRIIVIANLKPR
HHEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHCCCEEEEEECCCHH
VIRGFESQGMLLAAGCKEDEAKGIKPRILTVDGEVPPGTKIC
HHCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9380682; 10360571 [H]