Click here to switch to the map view.

The map label for this gene is infB

Identifier: 15897175

GI number: 15897175

Start: 197205

End: 199007

Strand: Direct

Name: infB

Synonym: SSO0228

Alternate gene names: 15897175

Gene position: 197205-199007 (Clockwise)

Preceding gene: 15897166

Following gene: 15897178

Centisome position: 6.59

GC content: 35.27

Gene sequence:

>1803_bases
ATGAAAATTAGCAACTCTGAGAGAAGGCTTAGGCAACCTATAGTAGTAGTATTAGGCCATGTAGATCATGGAAAAACTAC
ACTACTTGATAAAATAAGGGGTACAACAGTAGTTAAAAAGGAACCTGGAGAAATGACACAAGAGGTAGGAGCTAGTTTCG
TTCCAAGTTATATAATTGAAAAATTAGCAGAACCTCTTAAGAAGGTAATACCCATAAAACTTCAGATACCAGGATTATTG
TTTATTGACACGCCAGGTCATGAATATTTTTCAAACTTGAGAAGAAGAGGTGGAAGCGTAGCAGATATTGCGATCCTAGT
TGTTGATATAACTGAAGGCCTACAGAAGCAATCAATAGAATCAATACAAATACTAAGAGAAAGAAAAGTTCCATTTCTCA
TAGCTGCTAATAAAATAGATAAAATACCCGGATGGAAATCAAATAATGACATACCGTTTTTAGCATCAATCGAGAAACAG
AGGAATGATGTGAAAGTTTATCTAGACAACTTAGTCTATAATTTAGTTTCTCAATTAGCAAACTTAGGCTTTAGCTCAGA
ACGTTATGATAGAATAAAGGATTTCACTAAAACAGTAGCAATAGTTCCGGTTTCTGCAAAGACCGGTGAAGGCGTTGCAG
ATCTTTTAGCATTACTAGCTGGATTAACCCAAAGGTACTTAGAGACTAGATTAAAATTTGCAGAGGGTCCAGCAAAGGGA
GTTATATTAGAAGTAAAAGAAGATCCAGGGTTAGGACACACCATAGATGTTATAATTTATGATGGAGTGCTTAAGAAAAA
TGATACTATAATATTAGGAGGCATTAACGGCATTATTATAACTAAAGTTAGAGGAATATTTGTACCTAGACCATTACAAG
ATATGAAATTAAGCAAGTATGATCTAACGCCAATAGATGAAGTATATGCAGCAGCTGGAGTGAAGATATCTGCACCCAAT
TTAGAGGAAGCATTAGCTGGATCACCAATTTATGTGGTAGAAGACGAGTCTAAGGTTGAGCGATATAAACAACAGATAGA
AGAGGAAATTAAAGAAGTTAGACTCTACAGCGATATTGACGGAATAATACTCAAGGCAGATAGTTTAGGAACATTAGAAG
CCTTAGTTAGTGCTCTGCAGCGTGAAGGGATTCCAATAAGGCTAGCGGACATAGGGCCGATTTCGAAAAGAGATGTTATA
GAAGCGAGTATAGTAGCTCAAAGATCAAAAGAATATGGAATTATTGCTGCTTTTAGAGTAAAGTTATTACAAGGAATTGA
TACTAGTGGAATAAAAATATTGTATAACGAAATAATTTATCAATTAATCGAAGATATCAAGAAGCATATTAATGATGTCA
GGGAAGCGGAAAAAAGGCGCACGTTTGACACATTAATATTGCCAGGGAAAATAAAGATCTTGCCGGGTTATGTATTTAGA
CGCAGTGACCCAGTAGTAGTAGGTATTGAGGTTATAGGAGGCATTATAAGACCTAAGTATCCGTTAATTAAGGAAGATGG
AAGGAGAGTCGGTGAGGTACTACAAATCCAGGATAATAAGAAAAGTCTAGAAAGAGCCACTAAAGGAATGGAAGTTGCAA
TATCAATTAAAGGCAATATAATGATTGGGAGACATGTAAATGAAGGGGATGTTTTATACACAGACGTACCTAAAGAAGAC
CTCGAGATATTAGTCAACAAGTATCCAAGTTCTATTACAGATGATATGAGGGAAGTAATAAAAGAAATAATAAGAATAAA
GAGAAAAGAAGATCCTTTATATGGATTAGGATTACAGATCTGA

Upstream 100 bases:

>100_bases
TAATCATAACTATAATAATATTATTAAAAAGAAAGTTTGTCTTCCACGCAATTTTGTAGTAAACTAATTTATTCTAATAA
TACATTAAGGTCATAGGTGA

Downstream 100 bases:

>100_bases
TTTATCGAAGAATAACGACATCTCTCGCTGAGCTTTTTCAATAGAATCAGATGCATGAATGACGTTTTCTGACTTAGATA
ACGCATAATCTCCTCTTATA

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 600; Mature: 600

Protein sequence:

>600_residues
MKISNSERRLRQPIVVVLGHVDHGKTTLLDKIRGTTVVKKEPGEMTQEVGASFVPSYIIEKLAEPLKKVIPIKLQIPGLL
FIDTPGHEYFSNLRRRGGSVADIAILVVDITEGLQKQSIESIQILRERKVPFLIAANKIDKIPGWKSNNDIPFLASIEKQ
RNDVKVYLDNLVYNLVSQLANLGFSSERYDRIKDFTKTVAIVPVSAKTGEGVADLLALLAGLTQRYLETRLKFAEGPAKG
VILEVKEDPGLGHTIDVIIYDGVLKKNDTIILGGINGIIITKVRGIFVPRPLQDMKLSKYDLTPIDEVYAAAGVKISAPN
LEEALAGSPIYVVEDESKVERYKQQIEEEIKEVRLYSDIDGIILKADSLGTLEALVSALQREGIPIRLADIGPISKRDVI
EASIVAQRSKEYGIIAAFRVKLLQGIDTSGIKILYNEIIYQLIEDIKKHINDVREAEKRRTFDTLILPGKIKILPGYVFR
RSDPVVVGIEVIGGIIRPKYPLIKEDGRRVGEVLQIQDNKKSLERATKGMEVAISIKGNIMIGRHVNEGDVLYTDVPKED
LEILVNKYPSSITDDMREVIKEIIRIKRKEDPLYGLGLQI

Sequences:

>Translated_600_residues
MKISNSERRLRQPIVVVLGHVDHGKTTLLDKIRGTTVVKKEPGEMTQEVGASFVPSYIIEKLAEPLKKVIPIKLQIPGLL
FIDTPGHEYFSNLRRRGGSVADIAILVVDITEGLQKQSIESIQILRERKVPFLIAANKIDKIPGWKSNNDIPFLASIEKQ
RNDVKVYLDNLVYNLVSQLANLGFSSERYDRIKDFTKTVAIVPVSAKTGEGVADLLALLAGLTQRYLETRLKFAEGPAKG
VILEVKEDPGLGHTIDVIIYDGVLKKNDTIILGGINGIIITKVRGIFVPRPLQDMKLSKYDLTPIDEVYAAAGVKISAPN
LEEALAGSPIYVVEDESKVERYKQQIEEEIKEVRLYSDIDGIILKADSLGTLEALVSALQREGIPIRLADIGPISKRDVI
EASIVAQRSKEYGIIAAFRVKLLQGIDTSGIKILYNEIIYQLIEDIKKHINDVREAEKRRTFDTLILPGKIKILPGYVFR
RSDPVVVGIEVIGGIIRPKYPLIKEDGRRVGEVLQIQDNKKSLERATKGMEVAISIKGNIMIGRHVNEGDVLYTDVPKED
LEILVNKYPSSITDDMREVIKEIIRIKRKEDPLYGLGLQI
>Mature_600_residues
MKISNSERRLRQPIVVVLGHVDHGKTTLLDKIRGTTVVKKEPGEMTQEVGASFVPSYIIEKLAEPLKKVIPIKLQIPGLL
FIDTPGHEYFSNLRRRGGSVADIAILVVDITEGLQKQSIESIQILRERKVPFLIAANKIDKIPGWKSNNDIPFLASIEKQ
RNDVKVYLDNLVYNLVSQLANLGFSSERYDRIKDFTKTVAIVPVSAKTGEGVADLLALLAGLTQRYLETRLKFAEGPAKG
VILEVKEDPGLGHTIDVIIYDGVLKKNDTIILGGINGIIITKVRGIFVPRPLQDMKLSKYDLTPIDEVYAAAGVKISAPN
LEEALAGSPIYVVEDESKVERYKQQIEEEIKEVRLYSDIDGIILKADSLGTLEALVSALQREGIPIRLADIGPISKRDVI
EASIVAQRSKEYGIIAAFRVKLLQGIDTSGIKILYNEIIYQLIEDIKKHINDVREAEKRRTFDTLILPGKIKILPGYVFR
RSDPVVVGIEVIGGIIRPKYPLIKEDGRRVGEVLQIQDNKKSLERATKGMEVAISIKGNIMIGRHVNEGDVLYTDVPKED
LEILVNKYPSSITDDMREVIKEIIRIKRKEDPLYGLGLQI

Specific function: Function in general translation initiation by promoting the binding of the formylmethionine-tRNA to ribosomes. Seems to function along with eIF-2

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family

Homologues:

Organism=Homo sapiens, GI84043963, Length=595, Percent_Identity=36.8067226890756, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI53729339, Length=506, Percent_Identity=28.0632411067194, Blast_Score=139, Evalue=6e-33,
Organism=Homo sapiens, GI53729337, Length=506, Percent_Identity=28.0632411067194, Blast_Score=139, Evalue=6e-33,
Organism=Escherichia coli, GI1789559, Length=559, Percent_Identity=26.8336314847943, Blast_Score=119, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI212656558, Length=589, Percent_Identity=35.3140916808149, Blast_Score=364, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI71994658, Length=614, Percent_Identity=26.2214983713355, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6319282, Length=586, Percent_Identity=36.8600682593857, Blast_Score=347, Evalue=3e-96,
Organism=Saccharomyces cerevisiae, GI6324550, Length=575, Percent_Identity=25.9130434782609, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24656849, Length=576, Percent_Identity=35.2430555555556, Blast_Score=360, Evalue=2e-99,
Organism=Drosophila melanogaster, GI28572034, Length=489, Percent_Identity=28.6298568507157, Blast_Score=132, Evalue=9e-31,
Organism=Drosophila melanogaster, GI160714833, Length=87, Percent_Identity=43.6781609195402, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): IF2P_SULSO (Q980Q8)

Other databases:

- EMBL:   AE006641
- PIR:   C90164
- RefSeq:   NP_341780.1
- ProteinModelPortal:   Q980Q8
- SMR:   Q980Q8
- GeneID:   1455380
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0228
- NMPDR:   fig|273057.1.peg.203
- HOGENOM:   HBG635083
- OMA:   SPLRIVP
- PhylomeDB:   Q980Q8
- ProtClustDB:   PRK04004
- BioCyc:   SSOL273057:SSO0228-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_00100_A
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004544
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.40.50.10050
- PANTHER:   PTHR23115:SF41
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00491
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; SSF52156 TIF_IF2_dom3; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 66935; Mature: 66935

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS01176 IF2; PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISNSERRLRQPIVVVLGHVDHGKTTLLDKIRGTTVVKKEPGEMTQEVGASFVPSYIIE
CCCCCCHHHHCCCEEEEEECCCCCCHHHHHHHCCCEEEECCCCHHHHHHCHHHHHHHHHH
KLAEPLKKVIPIKLQIPGLLFIDTPGHEYFSNLRRRGGSVADIAILVVDITEGLQKQSIE
HHHHHHHHHCCEEEECCCEEEEECCCHHHHHHHHHCCCCEEEEEEEEEEHHHHHHHHHHH
SIQILRERKVPFLIAANKIDKIPGWKSNNDIPFLASIEKQRNDVKVYLDNLVYNLVSQLA
HHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHH
NLGFSSERYDRIKDFTKTVAIVPVSAKTGEGVADLLALLAGLTQRYLETRLKFAEGPAKG
HCCCCHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VILEVKEDPGLGHTIDVIIYDGVLKKNDTIILGGINGIIITKVRGIFVPRPLQDMKLSKY
EEEEEECCCCCCCEEEEEEECCEECCCCEEEEECCCEEEEEEECCEECCCCHHHCCCCCC
DLTPIDEVYAAAGVKISAPNLEEALAGSPIYVVEDESKVERYKQQIEEEIKEVRLYSDID
CCCCHHHHHHHCCCEEECCCHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCC
GIILKADSLGTLEALVSALQREGIPIRLADIGPISKRDVIEASIVAQRSKEYGIIAAFRV
CEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHH
KLLQGIDTSGIKILYNEIIYQLIEDIKKHINDVREAEKRRTFDTLILPGKIKILPGYVFR
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCEEEEECCEEEE
RSDPVVVGIEVIGGIIRPKYPLIKEDGRRVGEVLQIQDNKKSLERATKGMEVAISIKGNI
CCCCEEEEHHHHCCHHCCCCCCHHHCCHHHHHHEEECCCHHHHHHHCCCCEEEEEEECCE
MIGRHVNEGDVLYTDVPKEDLEILVNKYPSSITDDMREVIKEIIRIKRKEDPLYGLGLQI
EEEEECCCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKISNSERRLRQPIVVVLGHVDHGKTTLLDKIRGTTVVKKEPGEMTQEVGASFVPSYIIE
CCCCCCHHHHCCCEEEEEECCCCCCHHHHHHHCCCEEEECCCCHHHHHHCHHHHHHHHHH
KLAEPLKKVIPIKLQIPGLLFIDTPGHEYFSNLRRRGGSVADIAILVVDITEGLQKQSIE
HHHHHHHHHCCEEEECCCEEEEECCCHHHHHHHHHCCCCEEEEEEEEEEHHHHHHHHHHH
SIQILRERKVPFLIAANKIDKIPGWKSNNDIPFLASIEKQRNDVKVYLDNLVYNLVSQLA
HHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEHHHCCCCHHHHHHHHHHHHHHHHH
NLGFSSERYDRIKDFTKTVAIVPVSAKTGEGVADLLALLAGLTQRYLETRLKFAEGPAKG
HCCCCHHHHHHHHHHHHEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
VILEVKEDPGLGHTIDVIIYDGVLKKNDTIILGGINGIIITKVRGIFVPRPLQDMKLSKY
EEEEEECCCCCCCEEEEEEECCEECCCCEEEEECCCEEEEEEECCEECCCCHHHCCCCCC
DLTPIDEVYAAAGVKISAPNLEEALAGSPIYVVEDESKVERYKQQIEEEIKEVRLYSDID
CCCCHHHHHHHCCCEEECCCHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHCCCC
GIILKADSLGTLEALVSALQREGIPIRLADIGPISKRDVIEASIVAQRSKEYGIIAAFRV
CEEEECCCCCHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCHHHHHHH
KLLQGIDTSGIKILYNEIIYQLIEDIKKHINDVREAEKRRTFDTLILPGKIKILPGYVFR
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEEECCEEEEECCEEEE
RSDPVVVGIEVIGGIIRPKYPLIKEDGRRVGEVLQIQDNKKSLERATKGMEVAISIKGNI
CCCCEEEEHHHHCCHHCCCCCCHHHCCHHHHHHEEECCCHHHHHHHCCCCEEEEEEECCE
MIGRHVNEGDVLYTDVPKEDLEILVNKYPSSITDDMREVIKEIIRIKRKEDPLYGLGLQI
EEEEECCCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11427726