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The map label for this gene is rpoB1 [H]

Identifier: 15897172

GI number: 15897172

Start: 193195

End: 195144

Strand: Reverse

Name: rpoB1 [H]

Synonym: SSO0227

Alternate gene names: 15897172

Gene position: 195144-193195 (Counterclockwise)

Preceding gene: 15897173

Following gene: 15897171

Centisome position: 6.52

GC content: 36.87

Gene sequence:

>1950_bases
TTGCTGTACGGAATAAATGAGAGGATTGTAGAAAAAACACTTTATGAAATGGGAGTAGTTCCAGTGGAGGAGGTCATAAG
AAGAGTAACGGAAGGCGGAGAGGATCAGAATGAGTATCTGAAATGGTCTAAGGTTATACTCAATGGAAGATTAATAGGCT
ATTATCAAGATGGTGGAGAATTAGCTAATAAGATAAGAGAAAGAAGGAGAAAAGGAGAAATTAGTGATGAAGTAAACGTA
GGCCATATAGTGACAGATTTTATTAATGAGGTTCATGTTAATTGTGATTCTGGAAGAGTTAGAAGACCACTTATAATTGT
TTCTAACGGTAACCCGTTGGTAACTATTGAAGACATTGAAAAGTTAGAATCAGGTGCTATTACATTTGACGATCTTGTTA
GACAAGGAAAGATAGAGTATCTAGATGCAGAAGAAGAGGAGAACGCTTATGTTGCTTTAGAACCTAATGACTTAACTCCA
GATCATACTCATTTAGAAATATGGTCTCCAGCTATTTTAGGCATAACAGCGTCTATAATACCATATCCAGAGCATAATCA
ATCACCTAGAAATACATACCAATCAGCTATGGCGAAACAAGCTCTAGGTCTATATGCAGCAAATTATCAATTACGTACGG
ACACGAGAGCACATTTACTTCATTATCCACAAAGACCTCTAGTTCAAACTAGGGCATTAGATATTATAGGATATACAAAT
AGGCCGGCCGGAAATAATGCTATATTAGCCGTAATGTCATTTACTGGCTACAATATGGAAGATTCAATAATTATGAATAG
ATCCTCCGTGGAGAGGGGAATGTATAGATCTACATTTTTTAGGCTTTACTCAACGGAAGAGGTAAAATACCCTGGAGGTC
AAGAAGATAAAATAGTAATGCCAGAAGCTGGTGTTAGAGGATATAAGGGCAAAGAATATTACAGACTTCTAGAGGATAAC
GGAGTAGTCTCTCCAGAGGTCGAAGTGAAGGGAGGAGATGTTTTAATAGGTAAAGTTAGCCCTCCAAGATTCTTACAAGA
ATTTAAAGAATTATCTCCAGAGCAAGCTAAGCGTGACACCTCAATAGTTACAAGACATGGTGAAATGGGTATAGTGGATT
TAGTTCTAATTACCGAAACTGCTGAGGGTAATAAGCTAGTTAAGGTAAGAGTAAGAGATCTTAGGATACCAACAATTGGC
GATAAATTCGCCAGTAGACATGGACAAAAAGGCGTTATAGGTATGCTCATACCACAAGTTGACATGCCATATACCGTTAA
AGGCGTTGTGCCAGATATAATATTAAATCCTCATGCATTGCCATCTAGAATGACGTTAGGACAAATTATGGAAGGAATAG
CTGGTAAATATGCAGCATTATCCGGAAATATTGTAGATGCTACACCTTTCTACAAGACACCTATAGAACAATTACAAAAT
GAGATTTTGAGATACGGTTATCTACCAGATGCTACTGAAGTAGTGTATGATGGACGTACTGGACAGAAAATTAAATCTAG
AATATACTTTGGAGTAGTCTATTATCAGAAATTGCATCACATGGTAGCAGATAAGCTTCATGCTAGAGCTAGGGGTCCAG
TCCAAATTTTAACTAGACAACCAACAGAAGGAAGAGCTAGAGAAGGTGGTTTAAGATTTGGAGAAATGGAGAGAGATTGC
TTAATTGGTTTTGGTACTGCAATGCTTCTTAAAGACAGGTTATTGGATAACTCTGATAGGACAATGATTTACGTTTGTGA
TCAGTGTGGTTATATAGGCTGGTACGATAAGAATAAGAATAAATATGTATGCCCAATACATGGTGATAAGAGTAACTTGT
TCCCAGTTACTGTATCTTACGCATTTAAGCTTTTAATTCAAGAACTAATGAGTATGATTATCTCACCTAGGTTAGTTTTG
GAGGATAAAGTTGGATTAAGTGGAGGTTAA

Upstream 100 bases:

>100_bases
GAGATTTACATGGTACGCAATGGGGTAGGATGTGTCCCTTTGAAACACCAGAAGGTCCAAATAGTGGACTAGTTAAGAAT
CTAGCCGTTAATGGCTCAAA

Downstream 100 bases:

>100_bases
GGGAAATGAGTGAAAAGAATATAAAAGGAATAAAGTTTGGAATACTTTCTCCTGACGAAATAAGAAAAATGTCAGTGACT
GCAATAATAACACCAGACGT

Product: DNA-directed RNA polymerase subunit B'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 649; Mature: 649

Protein sequence:

>649_residues
MLYGINERIVEKTLYEMGVVPVEEVIRRVTEGGEDQNEYLKWSKVILNGRLIGYYQDGGELANKIRERRRKGEISDEVNV
GHIVTDFINEVHVNCDSGRVRRPLIIVSNGNPLVTIEDIEKLESGAITFDDLVRQGKIEYLDAEEEENAYVALEPNDLTP
DHTHLEIWSPAILGITASIIPYPEHNQSPRNTYQSAMAKQALGLYAANYQLRTDTRAHLLHYPQRPLVQTRALDIIGYTN
RPAGNNAILAVMSFTGYNMEDSIIMNRSSVERGMYRSTFFRLYSTEEVKYPGGQEDKIVMPEAGVRGYKGKEYYRLLEDN
GVVSPEVEVKGGDVLIGKVSPPRFLQEFKELSPEQAKRDTSIVTRHGEMGIVDLVLITETAEGNKLVKVRVRDLRIPTIG
DKFASRHGQKGVIGMLIPQVDMPYTVKGVVPDIILNPHALPSRMTLGQIMEGIAGKYAALSGNIVDATPFYKTPIEQLQN
EILRYGYLPDATEVVYDGRTGQKIKSRIYFGVVYYQKLHHMVADKLHARARGPVQILTRQPTEGRAREGGLRFGEMERDC
LIGFGTAMLLKDRLLDNSDRTMIYVCDQCGYIGWYDKNKNKYVCPIHGDKSNLFPVTVSYAFKLLIQELMSMIISPRLVL
EDKVGLSGG

Sequences:

>Translated_649_residues
MLYGINERIVEKTLYEMGVVPVEEVIRRVTEGGEDQNEYLKWSKVILNGRLIGYYQDGGELANKIRERRRKGEISDEVNV
GHIVTDFINEVHVNCDSGRVRRPLIIVSNGNPLVTIEDIEKLESGAITFDDLVRQGKIEYLDAEEEENAYVALEPNDLTP
DHTHLEIWSPAILGITASIIPYPEHNQSPRNTYQSAMAKQALGLYAANYQLRTDTRAHLLHYPQRPLVQTRALDIIGYTN
RPAGNNAILAVMSFTGYNMEDSIIMNRSSVERGMYRSTFFRLYSTEEVKYPGGQEDKIVMPEAGVRGYKGKEYYRLLEDN
GVVSPEVEVKGGDVLIGKVSPPRFLQEFKELSPEQAKRDTSIVTRHGEMGIVDLVLITETAEGNKLVKVRVRDLRIPTIG
DKFASRHGQKGVIGMLIPQVDMPYTVKGVVPDIILNPHALPSRMTLGQIMEGIAGKYAALSGNIVDATPFYKTPIEQLQN
EILRYGYLPDATEVVYDGRTGQKIKSRIYFGVVYYQKLHHMVADKLHARARGPVQILTRQPTEGRAREGGLRFGEMERDC
LIGFGTAMLLKDRLLDNSDRTMIYVCDQCGYIGWYDKNKNKYVCPIHGDKSNLFPVTVSYAFKLLIQELMSMIISPRLVL
EDKVGLSGG
>Mature_649_residues
MLYGINERIVEKTLYEMGVVPVEEVIRRVTEGGEDQNEYLKWSKVILNGRLIGYYQDGGELANKIRERRRKGEISDEVNV
GHIVTDFINEVHVNCDSGRVRRPLIIVSNGNPLVTIEDIEKLESGAITFDDLVRQGKIEYLDAEEEENAYVALEPNDLTP
DHTHLEIWSPAILGITASIIPYPEHNQSPRNTYQSAMAKQALGLYAANYQLRTDTRAHLLHYPQRPLVQTRALDIIGYTN
RPAGNNAILAVMSFTGYNMEDSIIMNRSSVERGMYRSTFFRLYSTEEVKYPGGQEDKIVMPEAGVRGYKGKEYYRLLEDN
GVVSPEVEVKGGDVLIGKVSPPRFLQEFKELSPEQAKRDTSIVTRHGEMGIVDLVLITETAEGNKLVKVRVRDLRIPTIG
DKFASRHGQKGVIGMLIPQVDMPYTVKGVVPDIILNPHALPSRMTLGQIMEGIAGKYAALSGNIVDATPFYKTPIEQLQN
EILRYGYLPDATEVVYDGRTGQKIKSRIYFGVVYYQKLHHMVADKLHARARGPVQILTRQPTEGRAREGGLRFGEMERDC
LIGFGTAMLLKDRLLDNSDRTMIYVCDQCGYIGWYDKNKNKYVCPIHGDKSNLFPVTVSYAFKLLIQELMSMIISPRLVL
EDKVGLSGG

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0085

COG function: function code K; DNA-directed RNA polymerase, beta subunit/140 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta chain family [H]

Homologues:

Organism=Homo sapiens, GI4505941, Length=610, Percent_Identity=46.3934426229508, Blast_Score=551, Evalue=1e-157,
Organism=Homo sapiens, GI238908505, Length=602, Percent_Identity=42.8571428571429, Blast_Score=486, Evalue=1e-137,
Organism=Homo sapiens, GI238908503, Length=602, Percent_Identity=42.8571428571429, Blast_Score=486, Evalue=1e-137,
Organism=Homo sapiens, GI33469941, Length=504, Percent_Identity=38.8888888888889, Blast_Score=323, Evalue=3e-88,
Organism=Homo sapiens, GI212286172, Length=500, Percent_Identity=38.6, Blast_Score=323, Evalue=4e-88,
Organism=Escherichia coli, GI1790419, Length=258, Percent_Identity=34.1085271317829, Blast_Score=129, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI17552304, Length=612, Percent_Identity=45.0980392156863, Blast_Score=555, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI25144348, Length=606, Percent_Identity=40.9240924092409, Blast_Score=459, Evalue=1e-129,
Organism=Caenorhabditis elegans, GI17506623, Length=544, Percent_Identity=35.1102941176471, Blast_Score=309, Evalue=3e-84,
Organism=Saccharomyces cerevisiae, GI6324725, Length=670, Percent_Identity=42.8358208955224, Blast_Score=543, Evalue=1e-155,
Organism=Saccharomyces cerevisiae, GI6324781, Length=606, Percent_Identity=41.9141914191419, Blast_Score=479, Evalue=1e-136,
Organism=Saccharomyces cerevisiae, GI6325267, Length=456, Percent_Identity=39.9122807017544, Blast_Score=311, Evalue=1e-85,
Organism=Drosophila melanogaster, GI17136444, Length=612, Percent_Identity=45.5882352941176, Blast_Score=549, Evalue=1e-156,
Organism=Drosophila melanogaster, GI17647877, Length=602, Percent_Identity=39.202657807309, Blast_Score=459, Evalue=1e-129,
Organism=Drosophila melanogaster, GI17136446, Length=539, Percent_Identity=35.2504638218924, Blast_Score=310, Evalue=3e-84,

Paralogues:

None

Copy number: 4233 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015712
- InterPro:   IPR007120
- InterPro:   IPR019969
- InterPro:   IPR007121
- InterPro:   IPR007644
- InterPro:   IPR007642
- InterPro:   IPR007645
- InterPro:   IPR007646
- InterPro:   IPR007647
- InterPro:   IPR007641
- InterPro:   IPR014724 [H]

Pfam domain/function: PF04563 RNA_pol_Rpb2_1; PF04561 RNA_pol_Rpb2_2; PF04565 RNA_pol_Rpb2_3; PF04566 RNA_pol_Rpb2_4; PF04567 RNA_pol_Rpb2_5; PF00562 RNA_pol_Rpb2_6; PF04560 RNA_pol_Rpb2_7 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 73125; Mature: 73125

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: PS01166 RNA_POL_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYGINERIVEKTLYEMGVVPVEEVIRRVTEGGEDQNEYLKWSKVILNGRLIGYYQDGGE
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHEEECCEEEEEECCCHH
LANKIRERRRKGEISDEVNVGHIVTDFINEVHVNCDSGRVRRPLIIVSNGNPLVTIEDIE
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEEEECCCCCCCCCEEEEECCCCEEEHHHHH
KLESGAITFDDLVRQGKIEYLDAEEEENAYVALEPNDLTPDHTHLEIWSPAILGITASII
HHHCCCCCHHHHHHCCCEEEECCCCCCCCEEEECCCCCCCCCCEEEECCCHHHHEEEEEE
PYPEHNQSPRNTYQSAMAKQALGLYAANYQLRTDTRAHLLHYPQRPLVQTRALDIIGYTN
CCCCCCCCHHHHHHHHHHHHHHHHEEECEEEECCCHHHHHCCCCCCCHHHHEEEEEECCC
RPAGNNAILAVMSFTGYNMEDSIIMNRSSVERGMYRSTFFRLYSTEEVKYPGGQEDKIVM
CCCCCCEEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHEEECCCEEECCCCCCCEEEC
PEAGVRGYKGKEYYRLLEDNGVVSPEVEVKGGDVLIGKVSPPRFLQEFKELSPEQAKRDT
CCCCCCCCCCHHHHHHHCCCCCCCCEEEECCCCEEEECCCCHHHHHHHHHCCHHHHHCCC
SIVTRHGEMGIVDLVLITETAEGNKLVKVRVRDLRIPTIGDKFASRHGQKGVIGMLIPQV
HHEEECCCCCEEEEEEEEECCCCCEEEEEEEEEEECCCCCHHHHHCCCCCCEEEEEECCC
DMPYTVKGVVPDIILNPHALPSRMTLGQIMEGIAGKYAALSGNIVDATPFYKTPIEQLQN
CCCEEEECCCCHHEECCCCCCCCCHHHHHHHHHCCCEEEECCCEEECCCCCCCHHHHHHH
EILRYGYLPDATEVVYDGRTGQKIKSRIYFGVVYYQKLHHMVADKLHARARGPVQILTRQ
HHHHHCCCCCCHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
PTEGRAREGGLRFGEMERDCLIGFGTAMLLKDRLLDNSDRTMIYVCDQCGYIGWYDKNKN
CCCCCCHHCCCCCCCCCCCCEEECHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCC
KYVCPIHGDKSNLFPVTVSYAFKLLIQELMSMIISPRLVLEDKVGLSGG
EEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC
>Mature Secondary Structure
MLYGINERIVEKTLYEMGVVPVEEVIRRVTEGGEDQNEYLKWSKVILNGRLIGYYQDGGE
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHEEECCEEEEEECCCHH
LANKIRERRRKGEISDEVNVGHIVTDFINEVHVNCDSGRVRRPLIIVSNGNPLVTIEDIE
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHEEEECCCCCCCCCEEEEECCCCEEEHHHHH
KLESGAITFDDLVRQGKIEYLDAEEEENAYVALEPNDLTPDHTHLEIWSPAILGITASII
HHHCCCCCHHHHHHCCCEEEECCCCCCCCEEEECCCCCCCCCCEEEECCCHHHHEEEEEE
PYPEHNQSPRNTYQSAMAKQALGLYAANYQLRTDTRAHLLHYPQRPLVQTRALDIIGYTN
CCCCCCCCHHHHHHHHHHHHHHHHEEECEEEECCCHHHHHCCCCCCCHHHHEEEEEECCC
RPAGNNAILAVMSFTGYNMEDSIIMNRSSVERGMYRSTFFRLYSTEEVKYPGGQEDKIVM
CCCCCCEEEEEEEECCCCCCCCEEEEHHHHHHHHHHHHHHEEECCCEEECCCCCCCEEEC
PEAGVRGYKGKEYYRLLEDNGVVSPEVEVKGGDVLIGKVSPPRFLQEFKELSPEQAKRDT
CCCCCCCCCCHHHHHHHCCCCCCCCEEEECCCCEEEECCCCHHHHHHHHHCCHHHHHCCC
SIVTRHGEMGIVDLVLITETAEGNKLVKVRVRDLRIPTIGDKFASRHGQKGVIGMLIPQV
HHEEECCCCCEEEEEEEEECCCCCEEEEEEEEEEECCCCCHHHHHCCCCCCEEEEEECCC
DMPYTVKGVVPDIILNPHALPSRMTLGQIMEGIAGKYAALSGNIVDATPFYKTPIEQLQN
CCCEEEECCCCHHEECCCCCCCCCHHHHHHHHHCCCEEEECCCEEECCCCCCCHHHHHHH
EILRYGYLPDATEVVYDGRTGQKIKSRIYFGVVYYQKLHHMVADKLHARARGPVQILTRQ
HHHHHCCCCCCHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEC
PTEGRAREGGLRFGEMERDCLIGFGTAMLLKDRLLDNSDRTMIYVCDQCGYIGWYDKNKN
CCCCCCHHCCCCCCCCCCCCEEECHHHHHHHHHHCCCCCCEEEEEECCCCEEEEEECCCC
KYVCPIHGDKSNLFPVTVSYAFKLLIQELMSMIISPRLVLEDKVGLSGG
EEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2501756 [H]