Click here to switch to the map view.

The map label for this gene is rpoB2 [H]

Identifier: 15897173

GI number: 15897173

Start: 195129

End: 196571

Strand: Reverse

Name: rpoB2 [H]

Synonym: SSO3254

Alternate gene names: 15897173

Gene position: 196571-195129 (Counterclockwise)

Preceding gene: 15897174

Following gene: 15897172

Centisome position: 6.57

GC content: 37.91

Gene sequence:

>1443_bases
TTGGCATCTAATTTAACCATTGATGAAAGATGGAGAGTCATCGAAGCGTACTTTAAATCCAAAGGCTTAGTTAGACAGCA
TCTGGATTCATACAATGACTTTGTTAGAAATAAGCTTCAAGAAATTATTGACGAACAAGGAGAAATACCCACAGAAATAC
CAGGTTTGAAAGTGAGATTAGGCAAGATAAGGATAGGAAAACCAAGAGTCCGGGAATCGGATAGAGGAGAAAGAGAAATC
AGTCCCATGGAAGCTCGATTAAGGAACTTAACTTATGCCGCTCCACTATGGCTTACAATGATCCCAGTTGAAAATAATAT
TGAAGCAGAACCAGAAGAGGTTTATATAGGCGACCTGCCTATAATGCTTAAATCAGCCATAGACCCAATATCACAGTATA
CTCTAGATAAGCTAATTGAGATAGGTGAAGATCCTAAAGACCCAGGAGGGTATTTCATAGTAAATGGATCTGAAAGAGTT
ATAGTAACTCAAGAAGATTTGGCTCCAAATAGAGTTCTTGTAGATACTGGAAAGACAGGATCAAACATTACGCATACAGC
GAAAATTATCTCGAGTACTGCGGGCTATAGAGTGCCTGTGACAATAGAAAGATTAAAAGATGGTACATTTCATGTATCTT
TTCCAGCAGTTCCCGGTAAGATTCCGTTTGTTATTCTAATGAGGGCACTGGGTATATTAACCGATAGAGATATAGTTTAT
GCGGTATCATTAGATCCTGAGGTTCAGAATGAATTATTTCCTTCTCTAGAGCAAGCAAGTTCGATAGCCAACGTTGATGA
TGCACTAGATTTTATAGGTAGTAGAGTAGCTATAGGCCAAAAGAGAGAAAACAGAATAGAAAAAGCACAGCAGATAATTG
ATAAATATTTCCTACCCCATTTAGGCACTTCAGCAGAAGATAGAAAGAAGAAAGCGTATTATTTAGCTTACGCTATATCA
AAAGTAATTGAATTATATCTTGGTAGAAGGGAACCCGACGATAAAGATCATTACGCTAACAAAAGATTAAGATTAGCTGG
AGATTTGTTTGCATCATTATTTAGAGTAGCTTTCAAAGCTTTCGTAAAAGATTTAACATATCAATTAGAGAAATCTAAGG
TAAGAGGTAGGAAACTCGCTTTAAAGGCATTAGTTAGACCAGATATTGTTACAGAAAGAATAAGGCATGCATTAGCTACT
GGGAACTGGGTTGGTGGAAGAACTGGAGTTAGCCAATTACTTGATAGGACCAACTGGCTTTCTATGTTAAGCCATCTGAG
GAGAGTAATATCCTCACTAGCAAGGGGTCAACCTAATTTCGAAGCCAGAGATTTACATGGTACGCAATGGGGTAGGATGT
GTCCCTTTGAAACACCAGAAGGTCCAAATAGTGGACTAGTTAAGAATCTAGCCGTTAATGGCTCAAATTGCTGTACGGAA
TAA

Upstream 100 bases:

>100_bases
GTATAAATGCGAAACCAGGGGATATAATTAGAATTATAAGGAAGAGTCAATTATATGGAGAAGTTGTCTCATATAGGTAT
GTAATTAGTGGGTGAACTAA

Downstream 100 bases:

>100_bases
ATGAGAGGATTGTAGAAAAAACACTTTATGAAATGGGAGTAGTTCCAGTGGAGGAGGTCATAAGAAGAGTAACGGAAGGC
GGAGAGGATCAGAATGAGTA

Product: DNA-directed RNA polymerase subunit beta''

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MASNLTIDERWRVIEAYFKSKGLVRQHLDSYNDFVRNKLQEIIDEQGEIPTEIPGLKVRLGKIRIGKPRVRESDRGEREI
SPMEARLRNLTYAAPLWLTMIPVENNIEAEPEEVYIGDLPIMLKSAIDPISQYTLDKLIEIGEDPKDPGGYFIVNGSERV
IVTQEDLAPNRVLVDTGKTGSNITHTAKIISSTAGYRVPVTIERLKDGTFHVSFPAVPGKIPFVILMRALGILTDRDIVY
AVSLDPEVQNELFPSLEQASSIANVDDALDFIGSRVAIGQKRENRIEKAQQIIDKYFLPHLGTSAEDRKKKAYYLAYAIS
KVIELYLGRREPDDKDHYANKRLRLAGDLFASLFRVAFKAFVKDLTYQLEKSKVRGRKLALKALVRPDIVTERIRHALAT
GNWVGGRTGVSQLLDRTNWLSMLSHLRRVISSLARGQPNFEARDLHGTQWGRMCPFETPEGPNSGLVKNLAVNGSNCCTE

Sequences:

>Translated_480_residues
MASNLTIDERWRVIEAYFKSKGLVRQHLDSYNDFVRNKLQEIIDEQGEIPTEIPGLKVRLGKIRIGKPRVRESDRGEREI
SPMEARLRNLTYAAPLWLTMIPVENNIEAEPEEVYIGDLPIMLKSAIDPISQYTLDKLIEIGEDPKDPGGYFIVNGSERV
IVTQEDLAPNRVLVDTGKTGSNITHTAKIISSTAGYRVPVTIERLKDGTFHVSFPAVPGKIPFVILMRALGILTDRDIVY
AVSLDPEVQNELFPSLEQASSIANVDDALDFIGSRVAIGQKRENRIEKAQQIIDKYFLPHLGTSAEDRKKKAYYLAYAIS
KVIELYLGRREPDDKDHYANKRLRLAGDLFASLFRVAFKAFVKDLTYQLEKSKVRGRKLALKALVRPDIVTERIRHALAT
GNWVGGRTGVSQLLDRTNWLSMLSHLRRVISSLARGQPNFEARDLHGTQWGRMCPFETPEGPNSGLVKNLAVNGSNCCTE
>Mature_479_residues
ASNLTIDERWRVIEAYFKSKGLVRQHLDSYNDFVRNKLQEIIDEQGEIPTEIPGLKVRLGKIRIGKPRVRESDRGEREIS
PMEARLRNLTYAAPLWLTMIPVENNIEAEPEEVYIGDLPIMLKSAIDPISQYTLDKLIEIGEDPKDPGGYFIVNGSERVI
VTQEDLAPNRVLVDTGKTGSNITHTAKIISSTAGYRVPVTIERLKDGTFHVSFPAVPGKIPFVILMRALGILTDRDIVYA
VSLDPEVQNELFPSLEQASSIANVDDALDFIGSRVAIGQKRENRIEKAQQIIDKYFLPHLGTSAEDRKKKAYYLAYAISK
VIELYLGRREPDDKDHYANKRLRLAGDLFASLFRVAFKAFVKDLTYQLEKSKVRGRKLALKALVRPDIVTERIRHALATG
NWVGGRTGVSQLLDRTNWLSMLSHLRRVISSLARGQPNFEARDLHGTQWGRMCPFETPEGPNSGLVKNLAVNGSNCCTE

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0085

COG function: function code K; DNA-directed RNA polymerase, beta subunit/140 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase beta chain family [H]

Homologues:

Organism=Homo sapiens, GI4505941, Length=504, Percent_Identity=38.2936507936508, Blast_Score=326, Evalue=3e-89,
Organism=Homo sapiens, GI238908503, Length=482, Percent_Identity=32.7800829875519, Blast_Score=263, Evalue=3e-70,
Organism=Homo sapiens, GI238908505, Length=424, Percent_Identity=33.0188679245283, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI33469941, Length=488, Percent_Identity=24.1803278688525, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1790419, Length=158, Percent_Identity=33.5443037974684, Blast_Score=92, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17552304, Length=501, Percent_Identity=37.9241516966068, Blast_Score=319, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI25144348, Length=493, Percent_Identity=30.8316430020284, Blast_Score=245, Evalue=4e-65,
Organism=Caenorhabditis elegans, GI17506623, Length=498, Percent_Identity=25.9036144578313, Blast_Score=109, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6324725, Length=517, Percent_Identity=37.5241779497099, Blast_Score=315, Evalue=9e-87,
Organism=Saccharomyces cerevisiae, GI6324781, Length=482, Percent_Identity=32.1576763485477, Blast_Score=240, Evalue=3e-64,
Organism=Saccharomyces cerevisiae, GI6325267, Length=454, Percent_Identity=24.8898678414097, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI17136444, Length=503, Percent_Identity=39.7614314115308, Blast_Score=339, Evalue=2e-93,
Organism=Drosophila melanogaster, GI17647877, Length=480, Percent_Identity=29.1666666666667, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI17136446, Length=512, Percent_Identity=22.65625, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: 4233 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015712
- InterPro:   IPR007120
- InterPro:   IPR019969
- InterPro:   IPR007121
- InterPro:   IPR007644
- InterPro:   IPR007642
- InterPro:   IPR007645
- InterPro:   IPR007646
- InterPro:   IPR007647
- InterPro:   IPR007641
- InterPro:   IPR014724 [H]

Pfam domain/function: PF04563 RNA_pol_Rpb2_1; PF04561 RNA_pol_Rpb2_2; PF04565 RNA_pol_Rpb2_3; PF04566 RNA_pol_Rpb2_4; PF04567 RNA_pol_Rpb2_5; PF00562 RNA_pol_Rpb2_6; PF04560 RNA_pol_Rpb2_7 [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 54036; Mature: 53905

Theoretical pI: Translated: 9.09; Mature: 9.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASNLTIDERWRVIEAYFKSKGLVRQHLDSYNDFVRNKLQEIIDEQGEIPTEIPGLKVRL
CCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
GKIRIGKPRVRESDRGEREISPMEARLRNLTYAAPLWLTMIPVENNIEAEPEEVYIGDLP
CEEEECCCCCCCCCCCCCCCCHHHHHHHHCCEECCEEEEEEECCCCCCCCCCEEEECCCH
IMLKSAIDPISQYTLDKLIEIGEDPKDPGGYFIVNGSERVIVTQEDLAPNRVLVDTGKTG
HHHHHHHCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEEECCCCCCEEEEECCCCC
SNITHTAKIISSTAGYRVPVTIERLKDGTFHVSFPAVPGKIPFVILMRALGILTDRDIVY
CCCHHHHHHHHCCCCCCCCEEHEECCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEE
AVSLDPEVQNELFPSLEQASSIANVDDALDFIGSRVAIGQKRENRIEKAQQIIDKYFLPH
EEECCHHHHHHHCCCHHHHHHHCCHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHC
LGTSAEDRKKKAYYLAYAISKVIELYLGRREPDDKDHYANKRLRLAGDLFASLFRVAFKA
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHH
FVKDLTYQLEKSKVRGRKLALKALVRPDIVTERIRHALATGNWVGGRTGVSQLLDRTNWL
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
SMLSHLRRVISSLARGQPNFEARDLHGTQWGRMCPFETPEGPNSGLVKNLAVNGSNCCTE
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEECCCCCCCC
>Mature Secondary Structure 
ASNLTIDERWRVIEAYFKSKGLVRQHLDSYNDFVRNKLQEIIDEQGEIPTEIPGLKVRL
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEE
GKIRIGKPRVRESDRGEREISPMEARLRNLTYAAPLWLTMIPVENNIEAEPEEVYIGDLP
CEEEECCCCCCCCCCCCCCCCHHHHHHHHCCEECCEEEEEEECCCCCCCCCCEEEECCCH
IMLKSAIDPISQYTLDKLIEIGEDPKDPGGYFIVNGSERVIVTQEDLAPNRVLVDTGKTG
HHHHHHHCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCEEEEEECCCCCCEEEEECCCCC
SNITHTAKIISSTAGYRVPVTIERLKDGTFHVSFPAVPGKIPFVILMRALGILTDRDIVY
CCCHHHHHHHHCCCCCCCCEEHEECCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCEEE
AVSLDPEVQNELFPSLEQASSIANVDDALDFIGSRVAIGQKRENRIEKAQQIIDKYFLPH
EEECCHHHHHHHCCCHHHHHHHCCHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHC
LGTSAEDRKKKAYYLAYAISKVIELYLGRREPDDKDHYANKRLRLAGDLFASLFRVAFKA
CCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHH
FVKDLTYQLEKSKVRGRKLALKALVRPDIVTERIRHALATGNWVGGRTGVSQLLDRTNWL
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
SMLSHLRRVISSLARGQPNFEARDLHGTQWGRMCPFETPEGPNSGLVKNLAVNGSNCCTE
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2501756 [H]