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The map label for this gene is rimK-1 [H]

Identifier: 15897110

GI number: 15897110

Start: 132570

End: 133430

Strand: Direct

Name: rimK-1 [H]

Synonym: SSO0159

Alternate gene names: 15897110

Gene position: 132570-133430 (Clockwise)

Preceding gene: 15897109

Following gene: 15897111

Centisome position: 4.43

GC content: 32.75

Gene sequence:

>861_bases
TTGGGGAGAGTGAAAATAGGAATTCTATATGACATGCCCAGATGGGAGGAAAAAAACCTTATTGAAGAGGGGAAAAAATT
AGGTTATCAAGTTGCTACTATATATAGTAAGGATTTTGTCCTTTTTTCAAATGATTTTACTATCGATAATGATACAGATC
TTTTTATCCAAAGAAACGTATCACATAACAGAGCCCTTATTACTTCATTTTTAGTAGAACAAGTTGGCTATCCTGTAATA
AATGACCACACGACTTTAATTAGATGTGAAAACAAAATTTTTACTACCTATATTTTAGCTAGACATAATATACCAACACC
TAAAACTTTCATAGCATTCGATAAGACAAATGCAATAGAATATTCAAAAAAGCTAGGCTATCCAGTAGTTATAAAGCCTG
TTGAAGGAAGTTGGGGAAGAATGGTAGCTAAAGCTGATAACTTAGACGTACTATATAGCTATCTAGAGTATCAAGAGTTT
AGCACGCAAAAATATAAGGATATATATTATATACAAGAATTCGTTAACAAACCAAATAGAGATATAAGGATATTCGTAAT
AGGAGACGAGACGCCAGTTGGAATTTACAGAGTTAACGAAAACAATTGGAGAACCAATACTGCATTAGGTGCAAAGGCCT
ATCCTCTTAAAATAGATGAGGAACTCAGAGAACTCGCATTAAAAGTAAAGGACATAATAGGGGGTTTCTTTTTAGGAATA
GACATTTTTGAAGATAAGGATCGTGGATACCTCGTAGATGAAGTTAATGGAGTACCAGAATACAAAAATACTGTAAGAGT
AAACAATTTTAATGTATCAAAATTCCTTTTAGAAAAGGCAGCGGAATGGGTGAAAAAATGA

Upstream 100 bases:

>100_bases
TACCAGGAGAACTTGTAGAACATGAATGCGGAGCTCAATTAGAAGTTGTAAAACAAAACGGCAAATTGTCACTTAGACTA
GCAGAGCAAATAGGTGAGGA

Downstream 100 bases:

>100_bases
TAAAGTTATTAAAATTCTATCAAGACAGAGGAATTAAAATTATTAAAGGAGAAGGGCAATACGTCTGGGATGAAAAGAAC
AATAAATATTTGGATATGCA

Product: ribosomal protein S6 modification protein (rimK-1)

Products: NA

Alternate protein names: AAA--lysW ligase lysX [H]

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MGRVKIGILYDMPRWEEKNLIEEGKKLGYQVATIYSKDFVLFSNDFTIDNDTDLFIQRNVSHNRALITSFLVEQVGYPVI
NDHTTLIRCENKIFTTYILARHNIPTPKTFIAFDKTNAIEYSKKLGYPVVIKPVEGSWGRMVAKADNLDVLYSYLEYQEF
STQKYKDIYYIQEFVNKPNRDIRIFVIGDETPVGIYRVNENNWRTNTALGAKAYPLKIDEELRELALKVKDIIGGFFLGI
DIFEDKDRGYLVDEVNGVPEYKNTVRVNNFNVSKFLLEKAAEWVKK

Sequences:

>Translated_286_residues
MGRVKIGILYDMPRWEEKNLIEEGKKLGYQVATIYSKDFVLFSNDFTIDNDTDLFIQRNVSHNRALITSFLVEQVGYPVI
NDHTTLIRCENKIFTTYILARHNIPTPKTFIAFDKTNAIEYSKKLGYPVVIKPVEGSWGRMVAKADNLDVLYSYLEYQEF
STQKYKDIYYIQEFVNKPNRDIRIFVIGDETPVGIYRVNENNWRTNTALGAKAYPLKIDEELRELALKVKDIIGGFFLGI
DIFEDKDRGYLVDEVNGVPEYKNTVRVNNFNVSKFLLEKAAEWVKK
>Mature_285_residues
GRVKIGILYDMPRWEEKNLIEEGKKLGYQVATIYSKDFVLFSNDFTIDNDTDLFIQRNVSHNRALITSFLVEQVGYPVIN
DHTTLIRCENKIFTTYILARHNIPTPKTFIAFDKTNAIEYSKKLGYPVVIKPVEGSWGRMVAKADNLDVLYSYLEYQEFS
TQKYKDIYYIQEFVNKPNRDIRIFVIGDETPVGIYRVNENNWRTNTALGAKAYPLKIDEELRELALKVKDIIGGFFLGID
IFEDKDRGYLVDEVNGVPEYKNTVRVNNFNVSKFLLEKAAEWVKK

Specific function: Catalyzes the ATP-dependent binding between the amino group of alpha-aminoadipate (AAA) and the gamma-carboxyl group of the C-terminal glutamate residue of lysW (Probable) [H]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI164663810, Length=216, Percent_Identity=26.3888888888889, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI134254462, Length=276, Percent_Identity=24.2753623188406, Blast_Score=68, Evalue=8e-12,
Organism=Escherichia coli, GI1787076, Length=219, Percent_Identity=26.9406392694064, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013651
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011870
- InterPro:   IPR016185
- InterPro:   IPR004666 [H]

Pfam domain/function: PF08443 RimK [H]

EC number: NA

Molecular weight: Translated: 33274; Mature: 33143

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRVKIGILYDMPRWEEKNLIEEGKKLGYQVATIYSKDFVLFSNDFTIDNDTDLFIQRNV
CCEEEEEEEECCCCCCHHHHHHHHHHCCEEEEEEEECCEEEEECCEEECCCCEEEEEECC
SHNRALITSFLVEQVGYPVINDHTTLIRCENKIFTTYILARHNIPTPKTFIAFDKTNAIE
CCCHHHHHHHHHHHCCCCEECCCEEEEEECCCEEEEEEEEECCCCCCCEEEEECCCCCHH
YSKKLGYPVVIKPVEGSWGRMVAKADNLDVLYSYLEYQEFSTQKYKDIYYIQEFVNKPNR
HHHHCCCCEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
DIRIFVIGDETPVGIYRVNENNWRTNTALGAKAYPLKIDEELRELALKVKDIIGGFFLGI
CEEEEEEECCCCEEEEEECCCCCEECCCCCCEECCEEHHHHHHHHHHHHHHHHCCEEEEE
DIFEDKDRGYLVDEVNGVPEYKNTVRVNNFNVSKFLLEKAAEWVKK
EEEECCCCCEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
GRVKIGILYDMPRWEEKNLIEEGKKLGYQVATIYSKDFVLFSNDFTIDNDTDLFIQRNV
CEEEEEEEECCCCCCHHHHHHHHHHCCEEEEEEEECCEEEEECCEEECCCCEEEEEECC
SHNRALITSFLVEQVGYPVINDHTTLIRCENKIFTTYILARHNIPTPKTFIAFDKTNAIE
CCCHHHHHHHHHHHCCCCEECCCEEEEEECCCEEEEEEEEECCCCCCCEEEEECCCCCHH
YSKKLGYPVVIKPVEGSWGRMVAKADNLDVLYSYLEYQEFSTQKYKDIYYIQEFVNKPNR
HHHHCCCCEEEEECCCCCCCEEEECCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCC
DIRIFVIGDETPVGIYRVNENNWRTNTALGAKAYPLKIDEELRELALKVKDIIGGFFLGI
CEEEEEEECCCCEEEEEECCCCCEECCCCCCEECCEEHHHHHHHHHHHHHHHHCCEEEEE
DIFEDKDRGYLVDEVNGVPEYKNTVRVNNFNVSKFLLEKAAEWVKK
EEEECCCCCEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Glu; Ribosomal protein S6 [C]

Specific reaction: Catalyzes the addition of glutamic acids to the carboxyl end of ribosomal protein S6 [C]

General reaction: Ligases; Forming Carbon-Nitrogen Bonds; Acid-D-Amino-Acid Ligases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11427726; 12042311 [H]