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The map label for this gene is gltX

Identifier: 15897055

GI number: 15897055

Start: 74314

End: 76041

Strand: Reverse

Name: gltX

Synonym: SSO0093

Alternate gene names: 15897055

Gene position: 76041-74314 (Counterclockwise)

Preceding gene: 15897056

Following gene: 15897054

Centisome position: 2.54

GC content: 35.71

Gene sequence:

>1728_bases
TTGATGGAATTAAATGAATTACGAGAAACTATTTATAAATATGCTCTTCAAAATGCGGTAAAGCATAATGGGAAAGCCGA
AACGGGTCCAGTGATGAGTAAGATTATAGCGGAGAGGCCGGAATTAAGATCAAATGCTAGAGAAATTGTAAAGATTATCA
AGGAGATCATAGAACAAGTTAACTCATTAACTTTAGAGCAGCAGTTAACTGAAATTAAAGCAAAGTATCCAGAGTTATTA
GAAGAGAAGAAGCATGAAGAAAAAAGAAAAGTGTTGCCACCTCTTCCTAATGTTAAAGGACAAGTAGTAACTAGATTTGC
CCCTAATCCGGATGGTCCTCTTCATTTAGGTAACGCAAGGTCTGCGATTCTATCTTACGAATACGCTAAAATGTATAACG
GTAAGTTCATACTGAGGTTTGATGATACAGATCCTAAGGTTAAAAGGCCAATTCTAGATGCATATGATTGGATTAAGGAG
GATCTAAAATGGCTGGGAATTAAATGGGAACAAGAACTTTACGCTTCGGAAAGGCTCGAGTTATACTATAAGTACGCTCG
GTATTTGATAGAAAAAGGATATGCGTATGTTGATACATGTGATAGTAGTATTTTCAGGAAGTTTAGGGATAGTAGAGGTA
AAATGAAAGAGCCAGAATGTTTACATAGGAGCTCTTCACCAGAGAGTAATCTGGAGCTGTTTGAAAAAATGCTAGGAGGA
AAGTTTAAAGAAGGAGAAGCTGTGGTTAGGTTAAAGACCGATCTATCGGATCCAGATCCATCTCAAATAGATTGGGTAAT
GCTTAGAATAATTGATACTGCTAAAAATCCTCATCCACGAGTAGGGAGCAAGTATTGGGTATGGCCTACCTATAACTTTG
CTTCTATAATAGATGATCATGAACTTGGAATTACTCATGTACTGAGAGCTAAGGAGCATATGTCAAATACAGAAAAGCAG
AGATACATTTCTGAGTACATGGGATGGGAATTTCCAGAGGTCTTACAGTTTGGAAGACTAAGACTTGAAGGTTTTATGAT
GAGTAAGTCGAAAATAAGAGGAATGTTAGAGAAGGGTACCAATAGGGATGATCCTAGATTGCCAACTTTAGCTGGACTCA
GAAGAAGAGGAATCTTACCAGATACAATTAAAGACGTAATAATTGATGTCGGAGTAAAGGTAACTGATGCTACTATAAGT
TTTGAGAACATTGCCGCAATTAATAGGAAAAAACTAGATCCGGTAGCTAAAAGAATAATGTTTGTAAAAGATGCAGAAGA
ATTTAGCGTCGAGCTACCTGAATCACTAAATGCTAAAATACCTTTAATTCCCTCTAAACAAGAAATGAATAGAACTATTA
TTGTCAATCCAGGAGATAAAATTTTGATAGAATCTAACGATGCAGAAGATAATAGTATACTAAGGTTAATGGAATTATGC
AATGTTAAAGTTGATAAGCATAATCGTAAGCTCATATTTCACAGCAAAACGTTAGACGAGGCTAAGAAAGTCAATGCAAA
GATAGTTCAATGGGTAAAATCAAATGAAAAAGTACCAGTAATGGTAGAGAAAGCGGAAAGAGATGAGATAAAAATGATAA
ATGGTTATGCTGAAAAAATAGCAGCAGATTTGGAGATAGACGAAATTGTACAATTTTACCGATTTGGATTTGTAAGAGTA
GATAGGAAAGATGAGAATATGCTACGTGTTGTATTCTCACACGACTGA

Upstream 100 bases:

>100_bases
AATGCTGATGATGAACTGAAAGATATAGCCGATTATATAACAAGTAATAAAAGTGGAAAAGGTGTAAGGGAATTTGTGGA
TAAATTACTAAAAGGGGAGT

Downstream 100 bases:

>100_bases
TTTCTTCTTTATTTCGTTAATGAATCCCTACCAATGATTCGATATTATATCTCTCGTAGAAAAACCAAAAGGTGGGAAAT
TCTCACATATAAAAATAAAG

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 575; Mature: 575

Protein sequence:

>575_residues
MMELNELRETIYKYALQNAVKHNGKAETGPVMSKIIAERPELRSNAREIVKIIKEIIEQVNSLTLEQQLTEIKAKYPELL
EEKKHEEKRKVLPPLPNVKGQVVTRFAPNPDGPLHLGNARSAILSYEYAKMYNGKFILRFDDTDPKVKRPILDAYDWIKE
DLKWLGIKWEQELYASERLELYYKYARYLIEKGYAYVDTCDSSIFRKFRDSRGKMKEPECLHRSSSPESNLELFEKMLGG
KFKEGEAVVRLKTDLSDPDPSQIDWVMLRIIDTAKNPHPRVGSKYWVWPTYNFASIIDDHELGITHVLRAKEHMSNTEKQ
RYISEYMGWEFPEVLQFGRLRLEGFMMSKSKIRGMLEKGTNRDDPRLPTLAGLRRRGILPDTIKDVIIDVGVKVTDATIS
FENIAAINRKKLDPVAKRIMFVKDAEEFSVELPESLNAKIPLIPSKQEMNRTIIVNPGDKILIESNDAEDNSILRLMELC
NVKVDKHNRKLIFHSKTLDEAKKVNAKIVQWVKSNEKVPVMVEKAERDEIKMINGYAEKIAADLEIDEIVQFYRFGFVRV
DRKDENMLRVVFSHD

Sequences:

>Translated_575_residues
MMELNELRETIYKYALQNAVKHNGKAETGPVMSKIIAERPELRSNAREIVKIIKEIIEQVNSLTLEQQLTEIKAKYPELL
EEKKHEEKRKVLPPLPNVKGQVVTRFAPNPDGPLHLGNARSAILSYEYAKMYNGKFILRFDDTDPKVKRPILDAYDWIKE
DLKWLGIKWEQELYASERLELYYKYARYLIEKGYAYVDTCDSSIFRKFRDSRGKMKEPECLHRSSSPESNLELFEKMLGG
KFKEGEAVVRLKTDLSDPDPSQIDWVMLRIIDTAKNPHPRVGSKYWVWPTYNFASIIDDHELGITHVLRAKEHMSNTEKQ
RYISEYMGWEFPEVLQFGRLRLEGFMMSKSKIRGMLEKGTNRDDPRLPTLAGLRRRGILPDTIKDVIIDVGVKVTDATIS
FENIAAINRKKLDPVAKRIMFVKDAEEFSVELPESLNAKIPLIPSKQEMNRTIIVNPGDKILIESNDAEDNSILRLMELC
NVKVDKHNRKLIFHSKTLDEAKKVNAKIVQWVKSNEKVPVMVEKAERDEIKMINGYAEKIAADLEIDEIVQFYRFGFVRV
DRKDENMLRVVFSHD
>Mature_575_residues
MMELNELRETIYKYALQNAVKHNGKAETGPVMSKIIAERPELRSNAREIVKIIKEIIEQVNSLTLEQQLTEIKAKYPELL
EEKKHEEKRKVLPPLPNVKGQVVTRFAPNPDGPLHLGNARSAILSYEYAKMYNGKFILRFDDTDPKVKRPILDAYDWIKE
DLKWLGIKWEQELYASERLELYYKYARYLIEKGYAYVDTCDSSIFRKFRDSRGKMKEPECLHRSSSPESNLELFEKMLGG
KFKEGEAVVRLKTDLSDPDPSQIDWVMLRIIDTAKNPHPRVGSKYWVWPTYNFASIIDDHELGITHVLRAKEHMSNTEKQ
RYISEYMGWEFPEVLQFGRLRLEGFMMSKSKIRGMLEKGTNRDDPRLPTLAGLRRRGILPDTIKDVIIDVGVKVTDATIS
FENIAAINRKKLDPVAKRIMFVKDAEEFSVELPESLNAKIPLIPSKQEMNRTIIVNPGDKILIESNDAEDNSILRLMELC
NVKVDKHNRKLIFHSKTLDEAKKVNAKIVQWVKSNEKVPVMVEKAERDEIKMINGYAEKIAADLEIDEIVQFYRFGFVRV
DRKDENMLRVVFSHD

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI62241042, Length=393, Percent_Identity=33.3333333333333, Blast_Score=195, Evalue=9e-50,
Organism=Homo sapiens, GI4826960, Length=353, Percent_Identity=33.7110481586402, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI134288884, Length=309, Percent_Identity=28.8025889967638, Blast_Score=112, Evalue=8e-25,
Organism=Escherichia coli, GI1786895, Length=523, Percent_Identity=30.9751434034417, Blast_Score=196, Evalue=4e-51,
Organism=Escherichia coli, GI1788743, Length=239, Percent_Identity=26.7782426778243, Blast_Score=103, Evalue=4e-23,
Organism=Escherichia coli, GI226510925, Length=284, Percent_Identity=26.056338028169, Blast_Score=87, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17511007, Length=406, Percent_Identity=32.0197044334975, Blast_Score=201, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17543332, Length=432, Percent_Identity=31.4814814814815, Blast_Score=171, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI71993394, Length=321, Percent_Identity=28.9719626168224, Blast_Score=102, Evalue=4e-22,
Organism=Saccharomyces cerevisiae, GI6324742, Length=452, Percent_Identity=33.1858407079646, Blast_Score=222, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6321192, Length=533, Percent_Identity=30.0187617260788, Blast_Score=186, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6324540, Length=317, Percent_Identity=31.2302839116719, Blast_Score=119, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24649466, Length=403, Percent_Identity=32.0099255583127, Blast_Score=178, Evalue=1e-44,
Organism=Drosophila melanogaster, GI17864488, Length=525, Percent_Identity=27.6190476190476, Blast_Score=156, Evalue=3e-38,
Organism=Drosophila melanogaster, GI24665321, Length=319, Percent_Identity=30.0940438871473, Blast_Score=113, Evalue=4e-25,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_SULSO (P95968)

Other databases:

- EMBL:   Y08257
- EMBL:   AE006641
- PIR:   S75395
- RefSeq:   NP_341660.1
- ProteinModelPortal:   P95968
- SMR:   P95968
- GeneID:   1453278
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0093
- NMPDR:   fig|273057.1.peg.83
- HOGENOM:   HBG334108
- OMA:   MRFAPNP
- PhylomeDB:   P95968
- ProtClustDB:   PRK04156
- BioCyc:   SSOL273057:SSO0093-MONOMER
- BRENDA:   6.1.1.17
- GO:   GO:0005737
- HAMAP:   MF_00022_A
- InterPro:   IPR004526
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR020059
- InterPro:   IPR022888
- InterPro:   IPR020056
- InterPro:   IPR011035
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:2.40.240.10
- Gene3D:   G3DSA:3.40.50.620
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00463

Pfam domain/function: PF00749 tRNA-synt_1c; PF03950 tRNA-synt_1c_C; SSF50715 Ribosomal_L25rel

EC number: =6.1.1.17

Molecular weight: Translated: 66806; Mature: 66806

Theoretical pI: Translated: 8.78; Mature: 8.78

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMELNELRETIYKYALQNAVKHNGKAETGPVMSKIIAERPELRSNAREIVKIIKEIIEQV
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
NSLTLEQQLTEIKAKYPELLEEKKHEEKRKVLPPLPNVKGQVVTRFAPNPDGPLHLGNAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCEEECCHH
SAILSYEYAKMYNGKFILRFDDTDPKVKRPILDAYDWIKEDLKWLGIKWEQELYASERLE
HHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LYYKYARYLIEKGYAYVDTCDSSIFRKFRDSRGKMKEPECLHRSSSPESNLELFEKMLGG
HHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHCC
KFKEGEAVVRLKTDLSDPDPSQIDWVMLRIIDTAKNPHPRVGSKYWVWPTYNFASIIDDH
CCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHCCC
ELGITHVLRAKEHMSNTEKQRYISEYMGWEFPEVLQFGRLRLEGFMMSKSKIRGMLEKGT
CCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NRDDPRLPTLAGLRRRGILPDTIKDVIIDVGVKVTDATISFENIAAINRKKLDPVAKRIM
CCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHCCHHHHHHH
FVKDAEEFSVELPESLNAKIPLIPSKQEMNRTIIVNPGDKILIESNDAEDNSILRLMELC
HHCCHHHHCEECCCCCCCCCCCCCCHHHCCCEEEECCCCEEEEECCCCCHHHHHHHHHHH
NVKVDKHNRKLIFHSKTLDEAKKVNAKIVQWVKSNEKVPVMVEKAERDEIKMINGYAEKI
CCEECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHH
AADLEIDEIVQFYRFGFVRVDRKDENMLRVVFSHD
HHCCCHHHHHHHHHHCEEEECCCCCCEEEEEEECC
>Mature Secondary Structure
MMELNELRETIYKYALQNAVKHNGKAETGPVMSKIIAERPELRSNAREIVKIIKEIIEQV
CCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
NSLTLEQQLTEIKAKYPELLEEKKHEEKRKVLPPLPNVKGQVVTRFAPNPDGPLHLGNAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCEEECCHH
SAILSYEYAKMYNGKFILRFDDTDPKVKRPILDAYDWIKEDLKWLGIKWEQELYASERLE
HHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
LYYKYARYLIEKGYAYVDTCDSSIFRKFRDSRGKMKEPECLHRSSSPESNLELFEKMLGG
HHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCCCCCCCHHHHCCCCCCHHHHHHHHHHCC
KFKEGEAVVRLKTDLSDPDPSQIDWVMLRIIDTAKNPHPRVGSKYWVWPTYNFASIIDDH
CCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCHHHHHCCC
ELGITHVLRAKEHMSNTEKQRYISEYMGWEFPEVLQFGRLRLEGFMMSKSKIRGMLEKGT
CCCHHHHHHHHHHHCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NRDDPRLPTLAGLRRRGILPDTIKDVIIDVGVKVTDATISFENIAAINRKKLDPVAKRIM
CCCCCCCCHHHHHHHCCCCHHHHHHHHHHCCCEEEEEEEEHHHHHHHHHHHCCHHHHHHH
FVKDAEEFSVELPESLNAKIPLIPSKQEMNRTIIVNPGDKILIESNDAEDNSILRLMELC
HHCCHHHHCEECCCCCCCCCCCCCCHHHCCCEEEECCCCEEEEECCCCCHHHHHHHHHHH
NVKVDKHNRKLIFHSKTLDEAKKVNAKIVQWVKSNEKVPVMVEKAERDEIKMINGYAEKI
CCEECCCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHH
AADLEIDEIVQFYRFGFVRVDRKDENMLRVVFSHD
HHCCCHHHHHHHHHHCEEEECCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8899719; 11427726