Click here to switch to the map view.

The map label for this gene is gnptA

Identifier: 15897027

GI number: 15897027

Start: 50238

End: 51206

Strand: Reverse

Name: gnptA

Synonym: SSO0060

Alternate gene names: 15897027

Gene position: 51206-50238 (Counterclockwise)

Preceding gene: 15897028

Following gene: 15897022

Centisome position: 1.71

GC content: 35.29

Gene sequence:

>969_bases
ATGAGAATACTTGCAATACTACTCCCAATTTTAATTTCCTTTTTCATAAGCTATATTACAACGGTCTGGGTAATAAGACA
AGCTAAAAAGAGTAGATTCGTAGGTAAGGATATAAATAAACCAGATAAGCCAGAAATACCATTATTGGGTGGGATAGGTA
TAATAGCTGGATTTATAGCCGGATCTTTCTCCTTATTATTAACTGATGTAAGGAGTGAAAGAGTTATTCCCGCCGTAATA
CTCTCCTCACTACTTATAGCGTTTCTTGGACTATTGGACGATATATTTAACGTTAGACAATCAGTAAGGGCTTTCTTGCC
AATTTTCGCATCAGTTCCACTAATAGTTTACAGTGTCGGGCATTCCATAATATCAATTCCGTTTTTAGGACCAATAAATT
TCGGAATATTCTATTATATTATAATAATACCATTTGCGCTAACTATAACATCTAACGCCTTTAATATGTTAGAAGGTCTA
AACGGATTAGGTGTAGGTATGGGAATAATAATGCTATCCGCACTGGCTTATATAGGGCTAACGCACACCGGTCCCACATA
TCAAGCTGGCTTAATAGCGCTTTCAGCAATTTTTTCACTATCGGCATTTCTCATATTTAATAAATATCCTGCGAAAATAT
TTCCAGGAAATGTTGGTACATACTTCATAGGAGCATTGATAGGAGCTATAGGAATTGCCGGCTTCATGTACACGGCATTA
GCAATTCTCTACATACCATACGTCGTGGAATTTATATTGAAGCTAAGAACTAACTTCAAGGGAGTATCATTTGGCAAAGT
AGACTCTAGCGGTAGATTATATTGGGATGAAAAACCTCATTCATTAACACATATAGTAATGAAAATGGGTCGATTTAAGG
AGTATCAAGTAGTTATAATTTTATGGGGAATGGAGGCAATATTTGCAGTAATAGCAGTAATTCTACAAACTACAACAATA
GTAATATAG

Upstream 100 bases:

>100_bases
TCTTGCAATAGAAAACTTACGTGAAATTCCTGTTTCTAATAAAACTGCTGAAAAGGCTTTAAAGTATCTAGCACAGTTTA
CCATTCAAAGGAGAAAGTAA

Downstream 100 bases:

>100_bases
TAAACTCTTGACCTTTATCAATACTCTTAAAAATAATAAGGATATATATAAAGATGGGGTCAGGGAGGGTGGGGGATCTC
GCCAATCCCTATTACCCGCA

Product: UDP-N-acetylglucosamine--dolichyl-phosphate N-acetylglucosaminephosphotransferase (gnptA)

Products: NA

Alternate protein names: GlcNAc-1-P transferase; G1PT; GPT; N-acetylglucosamine-1-phosphate transferase

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MRILAILLPILISFFISYITTVWVIRQAKKSRFVGKDINKPDKPEIPLLGGIGIIAGFIAGSFSLLLTDVRSERVIPAVI
LSSLLIAFLGLLDDIFNVRQSVRAFLPIFASVPLIVYSVGHSIISIPFLGPINFGIFYYIIIIPFALTITSNAFNMLEGL
NGLGVGMGIIMLSALAYIGLTHTGPTYQAGLIALSAIFSLSAFLIFNKYPAKIFPGNVGTYFIGALIGAIGIAGFMYTAL
AILYIPYVVEFILKLRTNFKGVSFGKVDSSGRLYWDEKPHSLTHIVMKMGRFKEYQVVIILWGMEAIFAVIAVILQTTTI
VI

Sequences:

>Translated_322_residues
MRILAILLPILISFFISYITTVWVIRQAKKSRFVGKDINKPDKPEIPLLGGIGIIAGFIAGSFSLLLTDVRSERVIPAVI
LSSLLIAFLGLLDDIFNVRQSVRAFLPIFASVPLIVYSVGHSIISIPFLGPINFGIFYYIIIIPFALTITSNAFNMLEGL
NGLGVGMGIIMLSALAYIGLTHTGPTYQAGLIALSAIFSLSAFLIFNKYPAKIFPGNVGTYFIGALIGAIGIAGFMYTAL
AILYIPYVVEFILKLRTNFKGVSFGKVDSSGRLYWDEKPHSLTHIVMKMGRFKEYQVVIILWGMEAIFAVIAVILQTTTI
VI
>Mature_322_residues
MRILAILLPILISFFISYITTVWVIRQAKKSRFVGKDINKPDKPEIPLLGGIGIIAGFIAGSFSLLLTDVRSERVIPAVI
LSSLLIAFLGLLDDIFNVRQSVRAFLPIFASVPLIVYSVGHSIISIPFLGPINFGIFYYIIIIPFALTITSNAFNMLEGL
NGLGVGMGIIMLSALAYIGLTHTGPTYQAGLIALSAIFSLSAFLIFNKYPAKIFPGNVGTYFIGALIGAIGIAGFMYTAL
AILYIPYVVEFILKLRTNFKGVSFGKVDSSGRLYWDEKPHSLTHIVMKMGRFKEYQVVIILWGMEAIFAVIAVILQTTTI
VI

Specific function: Catalyzes The Synthesis Of Und-Pp-Glcnac (Lipid I), The First Lipid-Linked Intermediate Involved In Eca Synthesis. This Lipid Is Also An Acceptor For The Addition Of Subsequent Sugars To Complete The Biosynthesis Of O-Antigen. [C]

COG id: COG0472

COG function: function code M; UDP-N-acetylmuramyl pentapeptide phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 4 family

Homologues:

Organism=Homo sapiens, GI42794009, Length=283, Percent_Identity=27.208480565371, Blast_Score=84, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319720, Length=300, Percent_Identity=27.6666666666667, Blast_Score=80, Evalue=6e-16,
Organism=Drosophila melanogaster, GI19921214, Length=276, Percent_Identity=26.0869565217391, Blast_Score=83, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GPT_SULSO (P96000)

Other databases:

- EMBL:   Y08257
- EMBL:   AE006641
- PIR:   S75428
- RefSeq:   NP_341632.1
- ProteinModelPortal:   P96000
- GeneID:   1455313
- GenomeReviews:   AE006641_GR
- KEGG:   sso:SSO0060
- NMPDR:   fig|273057.1.peg.55
- HOGENOM:   HBG553517
- OMA:   NMLAGFN
- ProtClustDB:   CLSK802540
- BioCyc:   SSOL273057:SSO0060-MONOMER
- BRENDA:   2.7.8.15
- InterPro:   IPR000715
- PANTHER:   PTHR22926

Pfam domain/function: PF00953 Glycos_transf_4

EC number: =2.7.8.15

Molecular weight: Translated: 35253; Mature: 35253

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x91f9ed4)-; HASH(0xbebd6d4)-; HASH(0xbed3dc4)-; HASH(0xbed7abc)-; HASH(0xbf2c548)-; HASH(0xbf43de4)-; HASH(0xbcc5fd4)-; HASH(0xbe809ec)-; HASH(0xbecfd84)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILAILLPILISFFISYITTVWVIRQAKKSRFVGKDINKPDKPEIPLLGGIGIIAGFIA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
GSFSLLLTDVRSERVIPAVILSSLLIAFLGLLDDIFNVRQSVRAFLPIFASVPLIVYSVG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HSIISIPFLGPINFGIFYYIIIIPFALTITSNAFNMLEGLNGLGVGMGIIMLSALAYIGL
HHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
THTGPTYQAGLIALSAIFSLSAFLIFNKYPAKIFPGNVGTYFIGALIGAIGIAGFMYTAL
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHH
AILYIPYVVEFILKLRTNFKGVSFGKVDSSGRLYWDEKPHSLTHIVMKMGRFKEYQVVII
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCEEEEEE
LWGMEAIFAVIAVILQTTTIVI
EECHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MRILAILLPILISFFISYITTVWVIRQAKKSRFVGKDINKPDKPEIPLLGGIGIIAGFIA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHH
GSFSLLLTDVRSERVIPAVILSSLLIAFLGLLDDIFNVRQSVRAFLPIFASVPLIVYSVG
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HSIISIPFLGPINFGIFYYIIIIPFALTITSNAFNMLEGLNGLGVGMGIIMLSALAYIGL
HHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCC
THTGPTYQAGLIALSAIFSLSAFLIFNKYPAKIFPGNVGTYFIGALIGAIGIAGFMYTAL
CCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHH
AILYIPYVVEFILKLRTNFKGVSFGKVDSSGRLYWDEKPHSLTHIVMKMGRFKEYQVVII
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHCCCCEEEEEE
LWGMEAIFAVIAVILQTTTIVI
EECHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8899719; 11427726