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The map label for this gene is paaF-1 [C]

Identifier: 15897020

GI number: 15897020

Start: 43863

End: 44612

Strand: Direct

Name: paaF-1 [C]

Synonym: SSO0050

Alternate gene names: 15897020

Gene position: 43863-44612 (Clockwise)

Preceding gene: 15897019

Following gene: 15897023

Centisome position: 1.47

GC content: 32.8

Gene sequence:

>750_bases
ATGCCTATTGAAGTTTATGAGTATGACAAATACATTTTATTGAAATTTTATCAAGCTGATAATAGATACAATCTATTTAA
TGTTGAGTTTATGACACAGATGATAGATGTTTTATCGGCAATCAATGAGAACAAGGGAAAAAGATTTCTCGTCATTAGAG
GAGAAGATAACTTTGGTGCAGGTGCAGATATAAGAGAATTAATTAAAGCCTCTAATGATAGTGAATTTGCAGTTACATTC
TTTACCTATATGAGGGAAGTATTTCATAAGATGCTTGATTTAAATAAAATAGTTATTTCTCAAGTGAAAAAAATAGCCTA
TGGAGCTTCTATGGAATTATTGTTATTCTCAGATTACGTGATATCAGAAAAAAAGGCAATGTTTGCAACACCGGGTGTGA
AAATAGGCGTATTTCCCCCAGTTCTCTCATCAGTTGGCCATTTTATATTAGGCTATAATAACGTTAAGAGAATTGCAATG
AAAGGTGATGTTATTGATACAAATGAGGCTAAATCTATTGGCCTAGTACATATTATTGACGACGACTTAGACAAGGCAAC
TTTAGATTTAATAGAAGAACTATCGACCCTTGCGCCTTCTGCAACATTGTATATAAAAAGAAATATGTTGAGGACATTTA
GAAATTATATTGATAAAGCCTTTGATGATCTTATTGTACAAATCCAGAGTGAGGAGGCAAGAGAGGGGCTGTTAAGCTTT
CTTAATAAGACTAATCCTCCTTGGATTTAG

Upstream 100 bases:

>100_bases
ATTAAATGACGAAGATAAGCGAAAAGTGAAAAATATATTGGACGAATTAAGTAAAGTTATGCGATAGATTTTTATTTGAT
AAGTTATATTTACTATCAAT

Downstream 100 bases:

>100_bases
CTAATTTCCCTTAACCTTAAAAGTCGCCCTCCCGCATAAAGGAAGACTATAGTAAAGAGGAAGACTATAGAGACCAGTAT
GGGATTAGGTGATATACCAT

Product: enoyl CoA hydratase (paaF-1)

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: Enoyl-CoA Hydratase; Enoyl-CoA Hydratase/Isomerase Family Protein; Short Chain Enoyl-CoA Hydratase; Gamma-Carboxygeranoyl-CoA Hydratase; Enoyl-CoA Hydratase/Carnithine Racemase; 2-Ketocyclohexanecarboxyl-CoA Hydrolase; 3-Hydroxybutyryl-CoA Dehydratase; Enoyl CoA Hydratase; Methylglutaconyl-CoA Hydratase; Carnitinyl-CoA Dehydratase; Enoyl-CoA Hydratase Protein; 3-Hydroxyacyl-CoA Dehydrogenase; Enoyl-Coa Hydratase; Enoyl-Coa Hydratase Protein; Enoyl-CoA Hydratase/Short Chain Enoyl-CoA Hydratase; 3-Hydroxybutyryl-CoA Dehydrogenase; Phenylacetate Degradation Enoyl-CoA Hydratase PaaB; 3-Hydroxyisobutyryl-CoA Hydrolase; Enoyl-CoA Hydratase-Isomerase; Fatty Acid-CoA Dehydratase; Cyclohexa-1 5-Dienecarbonyl-CoA Hydratase; Enoyl-CoA Hydratase/Isomerase PhaB; Cyclohex-1-Ene-1-Carboxyl-CoA Hydratase; Enoyl-CoA Hydratase Domain-Containing; 3-Hydroxybutyryl-CoA Dehydratase-Like Protein; 3-Hydroxybutyryl-COA Dehydratase

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MPIEVYEYDKYILLKFYQADNRYNLFNVEFMTQMIDVLSAINENKGKRFLVIRGEDNFGAGADIRELIKASNDSEFAVTF
FTYMREVFHKMLDLNKIVISQVKKIAYGASMELLLFSDYVISEKKAMFATPGVKIGVFPPVLSSVGHFILGYNNVKRIAM
KGDVIDTNEAKSIGLVHIIDDDLDKATLDLIEELSTLAPSATLYIKRNMLRTFRNYIDKAFDDLIVQIQSEEAREGLLSF
LNKTNPPWI

Sequences:

>Translated_249_residues
MPIEVYEYDKYILLKFYQADNRYNLFNVEFMTQMIDVLSAINENKGKRFLVIRGEDNFGAGADIRELIKASNDSEFAVTF
FTYMREVFHKMLDLNKIVISQVKKIAYGASMELLLFSDYVISEKKAMFATPGVKIGVFPPVLSSVGHFILGYNNVKRIAM
KGDVIDTNEAKSIGLVHIIDDDLDKATLDLIEELSTLAPSATLYIKRNMLRTFRNYIDKAFDDLIVQIQSEEAREGLLSF
LNKTNPPWI
>Mature_248_residues
PIEVYEYDKYILLKFYQADNRYNLFNVEFMTQMIDVLSAINENKGKRFLVIRGEDNFGAGADIRELIKASNDSEFAVTFF
TYMREVFHKMLDLNKIVISQVKKIAYGASMELLLFSDYVISEKKAMFATPGVKIGVFPPVLSSVGHFILGYNNVKRIAMK
GDVIDTNEAKSIGLVHIIDDDLDKATLDLIEELSTLAPSATLYIKRNMLRTFRNYIDKAFDDLIVQIQSEEAREGLLSFL
NKTNPPWI

Specific function: Catalyzes The Reversible Dehydration Of L-Carnitinyl-CoA To Crotobetainyl-CoA. [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI221142681, Length=210, Percent_Identity=28.0952380952381, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082183, Length=243, Percent_Identity=25.1028806584362, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17554946, Length=233, Percent_Identity=25.3218884120172, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI25145438, Length=241, Percent_Identity=26.5560165975104, Blast_Score=69, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 4.2.1.17

Molecular weight: Translated: 28466; Mature: 28335

Theoretical pI: Translated: 5.18; Mature: 5.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIEVYEYDKYILLKFYQADNRYNLFNVEFMTQMIDVLSAINENKGKRFLVIRGEDNFGA
CCCEEEECCCEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCC
GADIRELIKASNDSEFAVTFFTYMREVFHKMLDLNKIVISQVKKIAYGASMELLLFSDYV
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHH
ISEKKAMFATPGVKIGVFPPVLSSVGHFILGYNNVKRIAMKGDVIDTNEAKSIGLVHIID
HCCCCCEEECCCCEEECCHHHHHHHHHHHCCCCCCEEEEEECCEECCCCCCCEEEEEEEC
DDLDKATLDLIEELSTLAPSATLYIKRNMLRTFRNYIDKAFDDLIVQIQSEEAREGLLSF
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LNKTNPPWI
HHCCCCCCC
>Mature Secondary Structure 
PIEVYEYDKYILLKFYQADNRYNLFNVEFMTQMIDVLSAINENKGKRFLVIRGEDNFGA
CCEEEECCCEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCC
GADIRELIKASNDSEFAVTFFTYMREVFHKMLDLNKIVISQVKKIAYGASMELLLFSDYV
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHH
ISEKKAMFATPGVKIGVFPPVLSSVGHFILGYNNVKRIAMKGDVIDTNEAKSIGLVHIID
HCCCCCEEECCCCEEECCHHHHHHHHHHHCCCCCCEEEEEECCEECCCCCCCEEEEEEEC
DDLDKATLDLIEELSTLAPSATLYIKRNMLRTFRNYIDKAFDDLIVQIQSEEAREGLLSF
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
LNKTNPPWI
HHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA