Click here to switch to the map view.

The map label for this gene is bleO

Identifier: 15925739

GI number: 15925739

Start: 40197

End: 40601

Strand: Reverse

Name: bleO

Synonym: SA0032

Alternate gene names: 15925739

Gene position: 40601-40197 (Counterclockwise)

Preceding gene: 15925740

Following gene: 15925736

Centisome position: 1.44

GC content: 40.49

Gene sequence:

>405_bases
GTGAGAATGTTACAGTCTATCCCGGCATTGCCAGTCGGGGATATTAAAAAGAGTATAGGTTTTTATTGCGATAAACTAGG
TTTCACTTTGGTTCACCATGAAGATGGATTCGCAGTTCTAATGTGTAATGAGGTTCGGATTCATCTATGGGAGGCAAGTG
ATGAAGGCTGGCGCTCTCGTAGTAATGATTCACCGGTTTGTACAGGTGCGGAGTCGTTTATTGCTGGTACTGCTAGTTGC
CGCATTGAAGTAGAGGGAATTGATGAATTATATCAACATATTAAGCCTTTGGGCATTTTGCACCCCAATACATCATTAAA
AGATCAGTGGTGGGATGAACGAGACTTTGCAGTAATTGATCCCGACAACAATTTGATTAGCTTTTTTCAACAAATAAAAA
GCTAA

Upstream 100 bases:

>100_bases
CGGGCCATTTTGCGTAATAAGAAAAAGGATTAATTATGAGCGAATTGAATTAATAATAAGGTAATAGATTTACATTAGAA
AATGAAAGGGGATTTTATGC

Downstream 100 bases:

>100_bases
AATCTATTATTAATCTGTTCAGCAATCGGGCGCGATTGCTGAATAAAAGATACGAGAGACCTCTCTTGTATCTTTTTTAT
TTTGAGTGGTTTTGTCCGTT

Product: bleomycin resistance protein

Products: NA

Alternate protein names: BRP [H]

Number of amino acids: Translated: 134; Mature: 134

Protein sequence:

>134_residues
MRMLQSIPALPVGDIKKSIGFYCDKLGFTLVHHEDGFAVLMCNEVRIHLWEASDEGWRSRSNDSPVCTGAESFIAGTASC
RIEVEGIDELYQHIKPLGILHPNTSLKDQWWDERDFAVIDPDNNLISFFQQIKS

Sequences:

>Translated_134_residues
MRMLQSIPALPVGDIKKSIGFYCDKLGFTLVHHEDGFAVLMCNEVRIHLWEASDEGWRSRSNDSPVCTGAESFIAGTASC
RIEVEGIDELYQHIKPLGILHPNTSLKDQWWDERDFAVIDPDNNLISFFQQIKS
>Mature_134_residues
MRMLQSIPALPVGDIKKSIGFYCDKLGFTLVHHEDGFAVLMCNEVRIHLWEASDEGWRSRSNDSPVCTGAESFIAGTASC
RIEVEGIDELYQHIKPLGILHPNTSLKDQWWDERDFAVIDPDNNLISFFQQIKS

Specific function: Binding protein with a strong affinity to the bleomycin family of antibiotics [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bleomycin resistance protein family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000335
- InterPro:   IPR004360 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: NA

Molecular weight: Translated: 15209; Mature: 15209

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRMLQSIPALPVGDIKKSIGFYCDKLGFTLVHHEDGFAVLMCNEVRIHLWEASDEGWRSR
CCCHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCEEEEEEECCCCCCCCC
SNDSPVCTGAESFIAGTASCRIEVEGIDELYQHIKPLGILHPNTSLKDQWWDERDFAVID
CCCCCEEECCHHHHCCCCEEEEEECCHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEEEC
PDNNLISFFQQIKS
CCCHHHHHHHHHCC
>Mature Secondary Structure
MRMLQSIPALPVGDIKKSIGFYCDKLGFTLVHHEDGFAVLMCNEVRIHLWEASDEGWRSR
CCCHHHCCCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEEEECCEEEEEEECCCCCCCCC
SNDSPVCTGAESFIAGTASCRIEVEGIDELYQHIKPLGILHPNTSLKDQWWDERDFAVID
CCCCCEEECCHHHHCCCCEEEEEECCHHHHHHHHCCCEEECCCCCCCCCCCCCCCEEEEC
PDNNLISFFQQIKS
CCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2677995; 1946749 [H]