Click here to switch to the map view.

The map label for this gene is hisF

Identifier: 16127967

GI number: 16127967

Start: 3991946

End: 3992728

Strand: Direct

Name: hisF

Synonym: CC_3737

Alternate gene names: 16127967

Gene position: 3991946-3992728 (Clockwise)

Preceding gene: 16127966

Following gene: 16127968

Centisome position: 99.38

GC content: 69.6

Gene sequence:

>783_bases
ATGCTGAAGACCCGGATCATTCCCTGCCTCGACGTGAAGGACGGGCGGGTGGTCAAGGGGGTCAACTTCGTCTCCCTGCG
CGACGCCGGCGACCCGGTGGAGCAGGCGCGGGCCTATGACGCCGCCGGCGCCGACGAGCTGATGTTCCTGGACATCACCG
CTTCCAGCGAAGGACGCGGCCTGATCCTGGACGTCATCTCGCGCACCGCCGAGGTCTGCTTCATGCCCGTCTCGGTGGGC
GGCGGGGTGCGGCAGGTCTCAGACATGCGCCGCCTGTTGCTGGCCGGCGCCGACAAGGTCTCGGTCAACACCGCCGCCGT
CGAGAACCCCGACCTGATCGCCGGCGGCGCCGACGCCTTCGGGTCCCAGTGCGTGGTCGTGGCCATCGACGCTAAGGCGC
GCGAGGACGGCTCGGGCTGGAACGTCTGGACCTATGGCGGCCGCAAGGACACCGGGATCGACGTGGTCGAATGGGCCGCC
AAGGTCGTGGAACGCGGCGCGGGCGAGATCCTGCTGACCTCGATGGACCGCGACGGCGCCAAGATCGGCTACGATATCCC
GCTGCTGCAGGCGGTGACGGGCGCGGTCAATGTGCCGGTGATCGCCTCGGGCGGCGCGGGCAAGACCGAACATCTGATCG
AAGCCGCCCGCGAAGGCCACGCCGCCGCCGTGCTGGCCGCCTCGATCTTCCACTTCGGCGAGATTTCGATCGGCGAGGCC
AAGCAGGCCATGGCCGACGCCGGCATCCCCGTGCGCCTAGACGCGCTGAAGGGAGCCGCATGA

Upstream 100 bases:

>100_bases
AGGCCCGTCAGGGCGTCGAGATCGCCGGCGCGATCCTGGGCCGTTCGCTCTATGCCGGCACGATCAAGCCTTCCGAAGCC
CTGATCGCGGCGGCCGCCTG

Downstream 100 bases:

>100_bases
GCCAGCGCCTGACCGATGTCCTGCAGCGGCTGGCCGCGACCATCGAAGCCCGCAAGGGCGGCGATCCGTCGGTGTCCTAT
ACGGCCAAGCTGTTGAACGA

Product: cyclase HisF

Products: NA

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF

Number of amino acids: Translated: 260; Mature: 260

Protein sequence:

>260_residues
MLKTRIIPCLDVKDGRVVKGVNFVSLRDAGDPVEQARAYDAAGADELMFLDITASSEGRGLILDVISRTAEVCFMPVSVG
GGVRQVSDMRRLLLAGADKVSVNTAAVENPDLIAGGADAFGSQCVVVAIDAKAREDGSGWNVWTYGGRKDTGIDVVEWAA
KVVERGAGEILLTSMDRDGAKIGYDIPLLQAVTGAVNVPVIASGGAGKTEHLIEAAREGHAAAVLAASIFHFGEISIGEA
KQAMADAGIPVRLDALKGAA

Sequences:

>Translated_260_residues
MLKTRIIPCLDVKDGRVVKGVNFVSLRDAGDPVEQARAYDAAGADELMFLDITASSEGRGLILDVISRTAEVCFMPVSVG
GGVRQVSDMRRLLLAGADKVSVNTAAVENPDLIAGGADAFGSQCVVVAIDAKAREDGSGWNVWTYGGRKDTGIDVVEWAA
KVVERGAGEILLTSMDRDGAKIGYDIPLLQAVTGAVNVPVIASGGAGKTEHLIEAAREGHAAAVLAASIFHFGEISIGEA
KQAMADAGIPVRLDALKGAA
>Mature_260_residues
MLKTRIIPCLDVKDGRVVKGVNFVSLRDAGDPVEQARAYDAAGADELMFLDITASSEGRGLILDVISRTAEVCFMPVSVG
GGVRQVSDMRRLLLAGADKVSVNTAAVENPDLIAGGADAFGSQCVVVAIDAKAREDGSGWNVWTYGGRKDTGIDVVEWAA
KVVERGAGEILLTSMDRDGAKIGYDIPLLQAVTGAVNVPVIASGGAGKTEHLIEAAREGHAAAVLAASIFHFGEISIGEA
KQAMADAGIPVRLDALKGAA

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI1788336, Length=257, Percent_Identity=45.5252918287938, Blast_Score=216, Evalue=9e-58,
Organism=Escherichia coli, GI87082028, Length=239, Percent_Identity=29.7071129707113, Blast_Score=89, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6319725, Length=315, Percent_Identity=32.6984126984127, Blast_Score=140, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS6_CAUCN (B8GW15)

Other databases:

- EMBL:   CP001340
- RefSeq:   YP_002519226.1
- ProteinModelPortal:   B8GW15
- SMR:   B8GW15
- GeneID:   7332701
- GenomeReviews:   CP001340_GR
- KEGG:   ccs:CCNA_03853
- OMA:   RVVKGTN
- ProtClustDB:   PRK02083
- GO:   GO:0005737
- HAMAP:   MF_01013
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00735

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: 4.1.3.-

Molecular weight: Translated: 26928; Mature: 26928

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: ACT_SITE 11-11 ACT_SITE 130-130

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKTRIIPCLDVKDGRVVKGVNFVSLRDAGDPVEQARAYDAAGADELMFLDITASSEGRG
CCCCCEEEEEECCCCCEEECEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCCE
LILDVISRTAEVCFMPVSVGGGVRQVSDMRRLLLAGADKVSVNTAAVENPDLIAGGADAF
EHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEECCCCCC
GSQCVVVAIDAKAREDGSGWNVWTYGGRKDTGIDVVEWAAKVVERGAGEILLTSMDRDGA
CCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCC
KIGYDIPLLQAVTGAVNVPVIASGGAGKTEHLIEAAREGHAAAVLAASIFHFGEISIGEA
EECCCCHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHH
KQAMADAGIPVRLDALKGAA
HHHHHHCCCCEEEECCCCCC
>Mature Secondary Structure
MLKTRIIPCLDVKDGRVVKGVNFVSLRDAGDPVEQARAYDAAGADELMFLDITASSEGRG
CCCCCEEEEEECCCCCEEECEEEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCCE
LILDVISRTAEVCFMPVSVGGGVRQVSDMRRLLLAGADKVSVNTAAVENPDLIAGGADAF
EHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEECCCCCC
GSQCVVVAIDAKAREDGSGWNVWTYGGRKDTGIDVVEWAAKVVERGAGEILLTSMDRDGA
CCCEEEEEEECCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCC
KIGYDIPLLQAVTGAVNVPVIASGGAGKTEHLIEAAREGHAAAVLAASIFHFGEISIGEA
EECCCCHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCHH
KQAMADAGIPVRLDALKGAA
HHHHHHCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA