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The map label for this gene is maiA [H]

Identifier: 16127798

GI number: 16127798

Start: 3818688

End: 3819320

Strand: Direct

Name: maiA [H]

Synonym: CC_3568

Alternate gene names: 16127798

Gene position: 3818688-3819320 (Clockwise)

Preceding gene: 16127797

Following gene: 16127799

Centisome position: 95.06

GC content: 68.25

Gene sequence:

>633_bases
ATGAAGCTCGTCCTGCACAGCGCCAAGCGCGCCAGCGCGCCCTACCGAGTGCGCATCGGCCTAAACCTCAAGGGCCTTGC
GTTCGAGCTGCGCCCCGTCGACCTGGTGGCCAACGCCCATCAGGGCGAGGCCTACCGCGCGCTGAACGCCCAGGCCCTGG
TGCCGACGCTGGAGGTCGACGGGCGTCCGCTGACCCAGAGCCTGGCGATCCTGGAATGGCTGGACGAGACCTATCCCGCG
CATCCGCTGCTGCCGACCGACGCGTTTGACCGCGCCACGGTGCGGGCCATGGCCGAGATCATCGCCTGCGACATCCATCC
GCTGAACAACCTGCGCATCTTGCGCCAGCTGACCGCGCTTGAGATCGACGAGCCGGCGCGCAACGCCTGGGTCGCCCGCT
GGATCCAGGACGGCTTTTCCGCGTTGGAACCGATGATCGCGCGGCATGGCCAAGGCTACGCCTTTGGCGATGCGCCAGGC
TTGGTGGACTGTCTGCTGGTGCCCCAGGTGTTCAACGCCAACCGCTTCAATGTCGACCTGTCGCCGTTTCCGGCCCTGGA
AGCCGCCGCCGCGCATGCCCGGGCGCATCCGGCGATCGCCGCAGCTCACCCTGACCACCATCCGGAACGCTAG

Upstream 100 bases:

>100_bases
CGGCGACAAGGTCGTCGGCGAGATCGAGGGCGTCGGCCGCCTGGACTTCACCCTCGTCTAGCCGCGCAGCGCCGGTCCGC
GTCAAAGAGAAAAGGCTGAC

Downstream 100 bases:

>100_bases
ATGCTTGAAGACCTGAAAGCCAAGAACGCCGCCTGGTCGAAGAGCAAGACGGCGGAAGATCCGGATTTCTTTACACGCCT
GGCCAAGCAGCAAAGCCCGG

Product: glutathione S-transferase family protein

Products: NA

Alternate protein names: MAAI [H]

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MKLVLHSAKRASAPYRVRIGLNLKGLAFELRPVDLVANAHQGEAYRALNAQALVPTLEVDGRPLTQSLAILEWLDETYPA
HPLLPTDAFDRATVRAMAEIIACDIHPLNNLRILRQLTALEIDEPARNAWVARWIQDGFSALEPMIARHGQGYAFGDAPG
LVDCLLVPQVFNANRFNVDLSPFPALEAAAAHARAHPAIAAAHPDHHPER

Sequences:

>Translated_210_residues
MKLVLHSAKRASAPYRVRIGLNLKGLAFELRPVDLVANAHQGEAYRALNAQALVPTLEVDGRPLTQSLAILEWLDETYPA
HPLLPTDAFDRATVRAMAEIIACDIHPLNNLRILRQLTALEIDEPARNAWVARWIQDGFSALEPMIARHGQGYAFGDAPG
LVDCLLVPQVFNANRFNVDLSPFPALEAAAAHARAHPAIAAAHPDHHPER
>Mature_210_residues
MKLVLHSAKRASAPYRVRIGLNLKGLAFELRPVDLVANAHQGEAYRALNAQALVPTLEVDGRPLTQSLAILEWLDETYPA
HPLLPTDAFDRATVRAMAEIIACDIHPLNNLRILRQLTALEIDEPARNAWVARWIQDGFSALEPMIARHGQGYAFGDAPG
LVDCLLVPQVFNANRFNVDLSPFPALEAAAAHARAHPAIAAAHPDHHPER

Specific function: Forms An Equimolar Complex With The RNA Polymerase Holoenzyme (Rnap) But Not With The Core Enzyme. It Is Synthesized Predominantly When Cells Are Exposed To Amino Acid Starvation, At Which Time It Accounts For Over 50% Of The Total Protein Synthesized. It

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI22202624, Length=210, Percent_Identity=39.0476190476191, Blast_Score=152, Evalue=2e-37,
Organism=Homo sapiens, GI22202622, Length=156, Percent_Identity=39.7435897435897, Blast_Score=117, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17551302, Length=208, Percent_Identity=39.4230769230769, Blast_Score=152, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17510461, Length=210, Percent_Identity=35.2380952380952, Blast_Score=126, Evalue=7e-30,
Organism=Caenorhabditis elegans, GI17556142, Length=210, Percent_Identity=33.8095238095238, Blast_Score=119, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24645375, Length=210, Percent_Identity=39.5238095238095, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI21355859, Length=208, Percent_Identity=39.4230769230769, Blast_Score=168, Evalue=3e-42,
Organism=Drosophila melanogaster, GI45553325, Length=208, Percent_Identity=39.4230769230769, Blast_Score=168, Evalue=3e-42,
Organism=Drosophila melanogaster, GI21355857, Length=211, Percent_Identity=32.7014218009479, Blast_Score=130, Evalue=7e-31,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR005955
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: =5.2.1.2 [H]

Molecular weight: Translated: 23053; Mature: 23053

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLVLHSAKRASAPYRVRIGLNLKGLAFELRPVDLVANAHQGEAYRALNAQALVPTLEVD
CCEEECCHHHCCCCEEEEEECCCCCEEEEEECCEEEECCCCCCHHHHCCCCEECEEEECC
GRPLTQSLAILEWLDETYPAHPLLPTDAFDRATVRAMAEIIACDIHPLNNLRILRQLTAL
CCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
EIDEPARNAWVARWIQDGFSALEPMIARHGQGYAFGDAPGLVDCLLVPQVFNANRFNVDL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHCCCEEEEEC
SPFPALEAAAAHARAHPAIAAAHPDHHPER
CCCCHHHHHHHHHHCCCCEEECCCCCCCCC
>Mature Secondary Structure
MKLVLHSAKRASAPYRVRIGLNLKGLAFELRPVDLVANAHQGEAYRALNAQALVPTLEVD
CCEEECCHHHCCCCEEEEEECCCCCEEEEEECCEEEECCCCCCHHHHCCCCEECEEEECC
GRPLTQSLAILEWLDETYPAHPLLPTDAFDRATVRAMAEIIACDIHPLNNLRILRQLTAL
CCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
EIDEPARNAWVARWIQDGFSALEPMIARHGQGYAFGDAPGLVDCLLVPQVFNANRFNVDL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHCCCEEEEEC
SPFPALEAAAAHARAHPAIAAAHPDHHPER
CCCCHHHHHHHHHHCCCCEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]