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The map label for this gene is mexA [H]

Identifier: 16127349

GI number: 16127349

Start: 3352691

End: 3353755

Strand: Direct

Name: mexA [H]

Synonym: CC_3119

Alternate gene names: 16127349

Gene position: 3352691-3353755 (Clockwise)

Preceding gene: 16127346

Following gene: 16127350

Centisome position: 83.46

GC content: 74.18

Gene sequence:

>1065_bases
ATGTGGCGGACCCCGCTCCCTCCCCTGGCGGCCCTCCTCCTGGCGGCGACGTTGAGCGCCTGCGGCGGCAAGACCGAAAC
CGATGCGCCGGCCCCGCCGACGCCGGTCGAAGCCGCTCGCGTCGCCGCCCCTGACGCCGCCGGCGCGGTGACCGGCGCGG
GGGCGCTGGAGCGCCGCCGCGAAATGGCGCTGTCGTTCCGAATCCCCGGCGTTCTGACGACGATGAAGATCGAGGCCGGC
GACAGCGTCCGGGCCGGACAGCTGATCGCCGCCATCGACCCGGCCGGCGTCGACGCCCGCCAGCAACAGACCCAGGCCGA
CCTCGAGCGCGTGCGTCGCGACCTCGAGCGCGACAAGGTGCTGTTCGAGAAGGGCTATGTCAGCCGGCAGCGCATCGACG
ACCGGACAAGTGCGCTGAAGGCGGCCCAGGCCGCTTATGACGCCGCCCATTTCGACCGGCGTTGGGCCAATCTGGTCTCG
CCGGTCTCGGGCGTCGTGCTGGAACGCCGCGCCCAGGCGGGCGAGGTGGTCGGCGCGGGTCAGGTCGTCGCGCGGGTCGC
AGACCTGACCAGCCCGCTCGTTTTGCGCCTGCCGCTGGCCGCCCGCGACGCCGCCCGCATCCGCGTCGGGGACCCCGCCA
CGGTCAAGGTCGATGAGATCGGCGAGACCGCCCTGAACGGCCGCGTCACCCGCGTCGGCGAGGCCGCCGACGCCCGCACC
GGCGCCGTTCTGGTCGAGATCGAGCTTTCCGCGCCGCCCTCGACCTTGCGCAGCGGTCAGGTGGCCCACGCCACGCTCAA
TGTCCGCGCAGCCCCCGGCGTCACCACCACCTACGCCCGCGTGCCCGCCGAAGCGGTGCTGGAGGCCAATGGTCAGCGCG
CCTTCGTCTTCCGCGTCGACGGCGGCAAGGCGCGGCGCACGGCCGTGGGCTTTGGCGGCTTCGACGGCGACGACGCCCTG
GTCTCGGGCCTGCCGAACGGCGCCCAGGTCATCACCGCCGGCGCGGGCTTCGTCTCCGACGGCGAGGCCGTGGCCGTGGT
CGATCCCACACGGCTGGGGCGCTAG

Upstream 100 bases:

>100_bases
GGTGACTTCATTCAAGATGTCCTAGGCGTCGTCACGCCAGCTTGACAATACGCTGTTATCAATCACTGATAACTTATCGC
TGATTGATTTGGAACAGCTT

Downstream 100 bases:

>100_bases
GCCGTGAAGTTCGACCTCTCGACCTTCGCGGTTCGCCGCTGGCAGTTCACGCTGGTGGCCTTTGGCCTGCTGGTGATGCT
GGGCTTCAACGCCTTCACCA

Product: efflux system protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MWRTPLPPLAALLLAATLSACGGKTETDAPAPPTPVEAARVAAPDAAGAVTGAGALERRREMALSFRIPGVLTTMKIEAG
DSVRAGQLIAAIDPAGVDARQQQTQADLERVRRDLERDKVLFEKGYVSRQRIDDRTSALKAAQAAYDAAHFDRRWANLVS
PVSGVVLERRAQAGEVVGAGQVVARVADLTSPLVLRLPLAARDAARIRVGDPATVKVDEIGETALNGRVTRVGEAADART
GAVLVEIELSAPPSTLRSGQVAHATLNVRAAPGVTTTYARVPAEAVLEANGQRAFVFRVDGGKARRTAVGFGGFDGDDAL
VSGLPNGAQVITAGAGFVSDGEAVAVVDPTRLGR

Sequences:

>Translated_354_residues
MWRTPLPPLAALLLAATLSACGGKTETDAPAPPTPVEAARVAAPDAAGAVTGAGALERRREMALSFRIPGVLTTMKIEAG
DSVRAGQLIAAIDPAGVDARQQQTQADLERVRRDLERDKVLFEKGYVSRQRIDDRTSALKAAQAAYDAAHFDRRWANLVS
PVSGVVLERRAQAGEVVGAGQVVARVADLTSPLVLRLPLAARDAARIRVGDPATVKVDEIGETALNGRVTRVGEAADART
GAVLVEIELSAPPSTLRSGQVAHATLNVRAAPGVTTTYARVPAEAVLEANGQRAFVFRVDGGKARRTAVGFGGFDGDDAL
VSGLPNGAQVITAGAGFVSDGEAVAVVDPTRLGR
>Mature_354_residues
MWRTPLPPLAALLLAATLSACGGKTETDAPAPPTPVEAARVAAPDAAGAVTGAGALERRREMALSFRIPGVLTTMKIEAG
DSVRAGQLIAAIDPAGVDARQQQTQADLERVRRDLERDKVLFEKGYVSRQRIDDRTSALKAAQAAYDAAHFDRRWANLVS
PVSGVVLERRAQAGEVVGAGQVVARVADLTSPLVLRLPLAARDAARIRVGDPATVKVDEIGETALNGRVTRVGEAADART
GAVLVEIELSAPPSTLRSGQVAHATLNVRAAPGVTTTYARVPAEAVLEANGQRAFVFRVDGGKARRTAVGFGGFDGDDAL
VSGLPNGAQVITAGAGFVSDGEAVAVVDPTRLGR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor. Note=The membrane anchor is not necessary for antibiotic efflux as the protein is functional when targeted to the periplasm by a foreign signal peptide [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87082036, Length=340, Percent_Identity=25.8823529411765, Blast_Score=77, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 36766; Mature: 36766

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00398 RECOMBINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWRTPLPPLAALLLAATLSACGGKTETDAPAPPTPVEAARVAAPDAAGAVTGAGALERRR
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHH
EMALSFRIPGVLTTMKIEAGDSVRAGQLIAAIDPAGVDARQQQTQADLERVRRDLERDKV
HHHEEEECCCEEEEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
LFEKGYVSRQRIDDRTSALKAAQAAYDAAHFDRRWANLVSPVSGVVLERRAQAGEVVGAG
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
QVVARVADLTSPLVLRLPLAARDAARIRVGDPATVKVDEIGETALNGRVTRVGEAADART
HHHHHHHHCCCCEEEEECCCCCCCCEEEECCCCEEEHHCCCCHHHCCCEEEECCCCCCCC
GAVLVEIELSAPPSTLRSGQVAHATLNVRAAPGVTTTYARVPAEAVLEANGQRAFVFRVD
CEEEEEEECCCCCHHHHCCCEEEEEEEEEECCCCCCHHHHCCHHHHHHCCCCEEEEEEEC
GGKARRTAVGFGGFDGDDALVSGLPNGAQVITAGAGFVSDGEAVAVVDPTRLGR
CCCCEEEEEECCCCCCCHHHHHCCCCCCEEEEECCCCCCCCCEEEEECHHHCCC
>Mature Secondary Structure
MWRTPLPPLAALLLAATLSACGGKTETDAPAPPTPVEAARVAAPDAAGAVTGAGALERRR
CCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCHHHHHHHH
EMALSFRIPGVLTTMKIEAGDSVRAGQLIAAIDPAGVDARQQQTQADLERVRRDLERDKV
HHHEEEECCCEEEEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
LFEKGYVSRQRIDDRTSALKAAQAAYDAAHFDRRWANLVSPVSGVVLERRAQAGEVVGAG
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
QVVARVADLTSPLVLRLPLAARDAARIRVGDPATVKVDEIGETALNGRVTRVGEAADART
HHHHHHHHCCCCEEEEECCCCCCCCEEEECCCCEEEHHCCCCHHHCCCEEEECCCCCCCC
GAVLVEIELSAPPSTLRSGQVAHATLNVRAAPGVTTTYARVPAEAVLEANGQRAFVFRVD
CEEEEEEECCCCCHHHHCCCEEEEEEEEEECCCCCCHHHHCCHHHHHHCCCCEEEEEEEC
GGKARRTAVGFGGFDGDDALVSGLPNGAQVITAGAGFVSDGEAVAVVDPTRLGR
CCCCEEEEEECCCCCCCHHHHHCCCCCCEEEEECCCCCCCCCEEEEECHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7968531; 10984043; 9989496; 8226684; 8540696; 9603892; 10671490 [H]