Click here to switch to the map view.

The map label for this gene is ahpF [H]

Identifier: 16127149

GI number: 16127149

Start: 3139983

End: 3141566

Strand: Direct

Name: ahpF [H]

Synonym: CC_2919

Alternate gene names: 16127149

Gene position: 3139983-3141566 (Clockwise)

Preceding gene: 16127148

Following gene: 16127153

Centisome position: 78.17

GC content: 67.93

Gene sequence:

>1584_bases
ATGCTGGACGCCGGTCTGAAAGCCCAACTGACCACCTATCTGCAGAACCTGCGCCAGCCGATCACGCTGGTCGCCTCGCT
GGACGACTCCAAGGGCTCGGTCGACATGCGCGCCCTGCTGGACGACGTCGCCTCGACCTCCGACAAGGTCAGCGTCCGCT
TCGACGGCGATGACGCCCGCAAGCCCTCGTTCGCCATCGAGCGCGCCGCCAATGACGCCGAAGTGCGTTTCGCCGGCCTG
CCGCTGGGCCACGAGTTCACCTCGCTGGTGCTGGCTCTGCTGCACGTGGGCGGCCACCCGCCCCGCATCGACGCCGAGGT
GATCGAGCGCATCAAGGCGCTGGACGGCGACTATCAGTTCGAGACCTATTTCTCGCAGTCGTGCCAGAACTGCCCCGACG
TGGTGCAGGCGCTGAACACGATGAGCGCCCTGAACCCGCGCATCCGCCACGTCGCCATCGACGGCGCCCTGTTCAAGGCC
GAGGTCGAGGCGCGCCAGGTGATGGCCGTGCCGACCATCCTGCTGAACGGCCAGGCCTTCGGCCAGGGCCGCATGGAAAT
CGAACAGATCCTGGCCAAGCTGGACACCGGCGCAGCCGACCGCATGGCCGAAAAGATCAGGGCCAAGGACGCTTTCGAGG
TTCTGGTGGTCGGCGGCGGCCCGGCCGGCGCAGCGGCGGCGATCTACGCCGCCCGCAAGGGCATCCGCACCGGTGTGGCG
GCCGAACGCTTCGGCGGCCAGGTGCTGGACACCATGGATATCGAGAACTTCATCTCGGTGCCGCACACCGAAGGCCCCAA
GCTCGCCAGCGCGCTGGAGCAGCACGTCAAGGACTATGAGGTCGACGTGATGAACCTGCAGAAGGCCGTGGGCCTGGTCC
CGGCCAAGACGCCGGGCGGCCTGCACGAGATCAAGCTGGAGAACGGCGCCAACCTGAAGGCCCGCACCCTGATCCTGTCG
ACCGGCGCCCGCTGGCGCAACGTCAACGTGCCCGGCGAGGCCGAGTACAAGAACAAGGGCGTGGCCTACTGCCCGCACTG
CGACGGCCCGCTGTTCAAGGGCAAGCGCGTGGCGGTGATCGGCGGCGGCAACAGCGGCGTCGAGGCGGCCATCGATCTGG
CGGGCATCGTCGCCCACGTGACCCTGATCGAGTTCGACAGCCAACTGCGCGCCGACGCGGTGCTCCAGCGCAAGCTGGCC
AGCCTGCCGAACGTGACGATCATCAAATCGGCCATGACCACCGAGATCCACGGCGACGGGTCCAAGGTGAACGGCCTGAC
CTACAAGGATCGCACCAGCGACACGGCCCACCGCGTCGACCTGGAAGGCGTGTTCGTGCAGATCGGCCTGGTGCCCAACA
CCGAGTGGCTGAAGGACTCGGGCGTGGCCTTGTCGCCGCGCGGCGAGATCGAGGTCGACTATCGCGGCGAAACCTCGATC
CCCGGCGTCTTCGCCGCCGGCGACGCGACGGTGACGCCCTACAAGCAGATCATCATCGCCATGGGCGAAGGGGCCAAGGC
CTCGCTCTCGGCGTTCGACTACCTGATCCGCCTGCCGGCGTCGGAGGCGGCGGAAGCGGCGTAG

Upstream 100 bases:

>100_bases
GGGGCGGCGCGCCCTCTACCCGATCGTTCGTCGCGTTCGATCCGCCTCCCCGACAATCGAACATCCCCTTCCCCTCCCCT
CGCCTTTTTGGGAGCCCGCC

Downstream 100 bases:

>100_bases
GGGCGCACAACGCGTTGTCAGCCGACCTCATCGCCCAGCCAGGGCGGAAAGAGGTCGGCTAGCGCCTCAACAATATCTGC
TCCGAAAGTCTCGCCCTTAA

Product: alkyl hydroperoxide reductase subunit f

Products: an alcohol; H2O; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 527; Mature: 527

Protein sequence:

>527_residues
MLDAGLKAQLTTYLQNLRQPITLVASLDDSKGSVDMRALLDDVASTSDKVSVRFDGDDARKPSFAIERAANDAEVRFAGL
PLGHEFTSLVLALLHVGGHPPRIDAEVIERIKALDGDYQFETYFSQSCQNCPDVVQALNTMSALNPRIRHVAIDGALFKA
EVEARQVMAVPTILLNGQAFGQGRMEIEQILAKLDTGAADRMAEKIRAKDAFEVLVVGGGPAGAAAAIYAARKGIRTGVA
AERFGGQVLDTMDIENFISVPHTEGPKLASALEQHVKDYEVDVMNLQKAVGLVPAKTPGGLHEIKLENGANLKARTLILS
TGARWRNVNVPGEAEYKNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVAHVTLIEFDSQLRADAVLQRKLA
SLPNVTIIKSAMTTEIHGDGSKVNGLTYKDRTSDTAHRVDLEGVFVQIGLVPNTEWLKDSGVALSPRGEIEVDYRGETSI
PGVFAAGDATVTPYKQIIIAMGEGAKASLSAFDYLIRLPASEAAEAA

Sequences:

>Translated_527_residues
MLDAGLKAQLTTYLQNLRQPITLVASLDDSKGSVDMRALLDDVASTSDKVSVRFDGDDARKPSFAIERAANDAEVRFAGL
PLGHEFTSLVLALLHVGGHPPRIDAEVIERIKALDGDYQFETYFSQSCQNCPDVVQALNTMSALNPRIRHVAIDGALFKA
EVEARQVMAVPTILLNGQAFGQGRMEIEQILAKLDTGAADRMAEKIRAKDAFEVLVVGGGPAGAAAAIYAARKGIRTGVA
AERFGGQVLDTMDIENFISVPHTEGPKLASALEQHVKDYEVDVMNLQKAVGLVPAKTPGGLHEIKLENGANLKARTLILS
TGARWRNVNVPGEAEYKNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVAHVTLIEFDSQLRADAVLQRKLA
SLPNVTIIKSAMTTEIHGDGSKVNGLTYKDRTSDTAHRVDLEGVFVQIGLVPNTEWLKDSGVALSPRGEIEVDYRGETSI
PGVFAAGDATVTPYKQIIIAMGEGAKASLSAFDYLIRLPASEAAEAA
>Mature_527_residues
MLDAGLKAQLTTYLQNLRQPITLVASLDDSKGSVDMRALLDDVASTSDKVSVRFDGDDARKPSFAIERAANDAEVRFAGL
PLGHEFTSLVLALLHVGGHPPRIDAEVIERIKALDGDYQFETYFSQSCQNCPDVVQALNTMSALNPRIRHVAIDGALFKA
EVEARQVMAVPTILLNGQAFGQGRMEIEQILAKLDTGAADRMAEKIRAKDAFEVLVVGGGPAGAAAAIYAARKGIRTGVA
AERFGGQVLDTMDIENFISVPHTEGPKLASALEQHVKDYEVDVMNLQKAVGLVPAKTPGGLHEIKLENGANLKARTLILS
TGARWRNVNVPGEAEYKNKGVAYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVAHVTLIEFDSQLRADAVLQRKLA
SLPNVTIIKSAMTTEIHGDGSKVNGLTYKDRTSDTAHRVDLEGVFVQIGLVPNTEWLKDSGVALSPRGEIEVDYRGETSI
PGVFAAGDATVTPYKQIIIAMGEGAKASLSAFDYLIRLPASEAAEAA

Specific function: Serves to protect the cell against DNA damage by alkyl hydroperoxides. It can use either NADH or NADPH as electron donor for direct reduction of redox dyes or of alkyl hydroperoxides when combined with the AhpC protein [H]

COG id: COG3634

COG function: function code O; Alkyl hydroperoxide reductase, large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=301, Percent_Identity=27.2425249169435, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI226437568, Length=301, Percent_Identity=27.2425249169435, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI65787454, Length=301, Percent_Identity=27.2425249169435, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI87081763, Length=518, Percent_Identity=62.1621621621622, Blast_Score=652, Evalue=0.0,
Organism=Escherichia coli, GI1787114, Length=321, Percent_Identity=34.5794392523364, Blast_Score=151, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6321898, Length=316, Percent_Identity=31.6455696202532, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6320560, Length=327, Percent_Identity=30.2752293577982, Blast_Score=139, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012081
- InterPro:   IPR013027
- InterPro:   IPR002109
- InterPro:   IPR008255
- InterPro:   IPR001327
- InterPro:   IPR000103
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.6.4.- [C]

Molecular weight: Translated: 56200; Mature: 56200

Theoretical pI: Translated: 5.51; Mature: 5.51

Prosite motif: PS00573 PYRIDINE_REDOX_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDAGLKAQLTTYLQNLRQPITLVASLDDSKGSVDMRALLDDVASTSDKVSVRFDGDDAR
CCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCC
KPSFAIERAANDAEVRFAGLPLGHEFTSLVLALLHVGGHPPRIDAEVIERIKALDGDYQF
CCCHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCH
ETYFSQSCQNCPDVVQALNTMSALNPRIRHVAIDGALFKAEVEARQVMAVPTILLNGQAF
HHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCEEEEEHHHHHHHEEECEEEECCCCC
GQGRMEIEQILAKLDTGAADRMAEKIRAKDAFEVLVVGGGPAGAAAAIYAARKGIRTGVA
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCH
AERFGGQVLDTMDIENFISVPHTEGPKLASALEQHVKDYEVDVMNLQKAVGLVPAKTPGG
HHHHCCCEEEHHCHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCC
LHEIKLENGANLKARTLILSTGARWRNVNVPGEAEYKNKGVAYCPHCDGPLFKGKRVAVI
CEEEEECCCCCCEEEEEEEECCCCEEECCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEE
GGGNSGVEAAIDLAGIVAHVTLIEFDSQLRADAVLQRKLASLPNVTIIKSAMTTEIHGDG
ECCCCCHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHCCCCEEEEEHHHEEEECCCC
SKVNGLTYKDRTSDTAHRVDLEGVFVQIGLVPNTEWLKDSGVALSPRGEIEVDYRGETSI
CEECCCEECCCCCCCHHEEEECEEEEEEEECCCCHHHCCCCEEECCCCCEEEEECCCCCC
PGVFAAGDATVTPYKQIIIAMGEGAKASLSAFDYLIRLPASEAAEAA
CCEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCHHHCCCC
>Mature Secondary Structure
MLDAGLKAQLTTYLQNLRQPITLVASLDDSKGSVDMRALLDDVASTSDKVSVRFDGDDAR
CCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCC
KPSFAIERAANDAEVRFAGLPLGHEFTSLVLALLHVGGHPPRIDAEVIERIKALDGDYQF
CCCHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCH
ETYFSQSCQNCPDVVQALNTMSALNPRIRHVAIDGALFKAEVEARQVMAVPTILLNGQAF
HHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECCEEEEEHHHHHHHEEECEEEECCCCC
GQGRMEIEQILAKLDTGAADRMAEKIRAKDAFEVLVVGGGPAGAAAAIYAARKGIRTGVA
CCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCH
AERFGGQVLDTMDIENFISVPHTEGPKLASALEQHVKDYEVDVMNLQKAVGLVPAKTPGG
HHHHCCCEEEHHCHHHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCC
LHEIKLENGANLKARTLILSTGARWRNVNVPGEAEYKNKGVAYCPHCDGPLFKGKRVAVI
CEEEEECCCCCCEEEEEEEECCCCEEECCCCCCCCCCCCCCEECCCCCCCCCCCCEEEEE
GGGNSGVEAAIDLAGIVAHVTLIEFDSQLRADAVLQRKLASLPNVTIIKSAMTTEIHGDG
ECCCCCHHHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHCCCCEEEEEHHHEEEECCCC
SKVNGLTYKDRTSDTAHRVDLEGVFVQIGLVPNTEWLKDSGVALSPRGEIEVDYRGETSI
CEECCCEECCCCCCCHHEEEECEEEEEEEECCCCHHHCCCCEEECCCCCEEEEECCCCCC
PGVFAAGDATVTPYKQIIIAMGEGAKASLSAFDYLIRLPASEAAEAA
CCEEECCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ROOH; Proton; NADH [C]

Specific reaction: ROOH + Proton + NADH = an alcohol + H2O + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]