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The map label for this gene is purB [H]

Identifier: 16126726

GI number: 16126726

Start: 2698555

End: 2699865

Strand: Direct

Name: purB [H]

Synonym: CC_2487

Alternate gene names: 16126726

Gene position: 2698555-2699865 (Clockwise)

Preceding gene: 16126725

Following gene: 16126727

Centisome position: 67.18

GC content: 66.51

Gene sequence:

>1311_bases
ATGATCAGCCGCTACGCCCGCCCCGAAGCCGCCGCCATCTGGTCCAGCCAGACCAAGTACAAGATCTGGTTCGAGATCGA
GGCCCACGCCGCCGACGCCATGGCCGAGCTGGGAGTCATCCCCAAGCTCGCCGCCGAGACGATCTGGGAAAAGGGCCGCG
ACGCGGTCTGGGACAGCGACCGCATCGATGAGATCGAGCGCGTCACTAAGCACGACGTCATCGCCTTCCTGACCCACGTC
TCCGAGATCGTCGGTCCCGAAGCCCGCTTCCTGCACCAGGGCATGACCAGCAGCGACGTGCTGGACACCTGCTTCGCCGT
GCAGCTGTCGCGCGCCACCGACCTGCTGCTGGAAGACGTCGATCTGGTGCTGGCCGCGCTGAAGCGCCGGGCCCTGGAAC
ACAAGATGACCGTCTGCGTCGGCCGCAGCCACGGCATCCACGCTGAGCCGATCACCTTTGGCCTGAAGCTCGCAGGCTAC
TACGCCGAGTTCCAGCGCGCCAAGGAGCGCCTGGCGATGGCCAAGTTCGAGATCGCCACCTGCGCGATCTCGGGCGCGGT
GGGCACCTTCGCCAATGTCGATCCGCGCGTCGAACAGCACGTGGCCGACAAGATGGGCCTGGCTGTCGAGCCGGTCTCGA
CCCAGGTGATCCCGCGCGACCGCCACGCGGCCTATTTCGCGGCCCTGGGCGTGGTGGCCTCGTCGGTCGAGCGCCTGGCC
ACCGAGATCCGCCACCTGCAGCGCACCGAGGTGCTGGAAGCCGAAGAGCCGTTCGATCCGGGCCAGAAGGGCTCGTCGGC
CATGCCGCACAAGCGCAACCCGATCCTGACCGAAAACCTCACCGGCCTGGCGCGCTTGGTCCGTTCGGCCGTGGTGCCGG
CGATGGAGAACGTCGCCCTCTGGCACGAGCGCGACATCAGCCACTCGTCGGTCGAGCGCGGCATCGGCCCCGACGCCACC
ATCCACCTCGACTTCGCCCTGCGCCGTCTGGCCGGCGTGATCGAGCGCTTCAACATCTATCCCGACAACATGGCCAAGAA
CCTGGACAAGTTGGGGGGTCTCGTTTTCTCCCAGCGCGTCATGCTGGCCCTGACCCACAAGGACGTCTCCCGCGAGGACG
CCTATGCGGCCGTGCAGGGCAACGCCATGAAGGTCTGGCGCGGCGAAGGCCGGTTCCTCGACTTCCTGAAGGCCGATCCG
GTCGTGTCCAAGGCCCTGACCGAGACCGAACTCGAAGAGCTGTTCGACCTTGGCTACCACACCAAGAATGTCGACGTGAT
CTTTCAGCGCGTCTTCGGCGAACAGGGCTGA

Upstream 100 bases:

>100_bases
CGTCCGCTGCGCGGACACCTCCCCCGCTGGGGGGAGGATTGGCCTAAGTAATAAGCCTCTTCCCTTGACTCAGCGACCTG
CCCGGCCGAAACGCCCGCCC

Downstream 100 bases:

>100_bases
GCATGAGCCAGCCGCCGATCCTGCGCCTCAATCCCGCGCTGGATCCGGCGGCGTTCGCGCCCGCCTACGCCCGCGACGGC
GTGGTGCGCATCCCCGAGGT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MISRYARPEAAAIWSSQTKYKIWFEIEAHAADAMAELGVIPKLAAETIWEKGRDAVWDSDRIDEIERVTKHDVIAFLTHV
SEIVGPEARFLHQGMTSSDVLDTCFAVQLSRATDLLLEDVDLVLAALKRRALEHKMTVCVGRSHGIHAEPITFGLKLAGY
YAEFQRAKERLAMAKFEIATCAISGAVGTFANVDPRVEQHVADKMGLAVEPVSTQVIPRDRHAAYFAALGVVASSVERLA
TEIRHLQRTEVLEAEEPFDPGQKGSSAMPHKRNPILTENLTGLARLVRSAVVPAMENVALWHERDISHSSVERGIGPDAT
IHLDFALRRLAGVIERFNIYPDNMAKNLDKLGGLVFSQRVMLALTHKDVSREDAYAAVQGNAMKVWRGEGRFLDFLKADP
VVSKALTETELEELFDLGYHTKNVDVIFQRVFGEQG

Sequences:

>Translated_436_residues
MISRYARPEAAAIWSSQTKYKIWFEIEAHAADAMAELGVIPKLAAETIWEKGRDAVWDSDRIDEIERVTKHDVIAFLTHV
SEIVGPEARFLHQGMTSSDVLDTCFAVQLSRATDLLLEDVDLVLAALKRRALEHKMTVCVGRSHGIHAEPITFGLKLAGY
YAEFQRAKERLAMAKFEIATCAISGAVGTFANVDPRVEQHVADKMGLAVEPVSTQVIPRDRHAAYFAALGVVASSVERLA
TEIRHLQRTEVLEAEEPFDPGQKGSSAMPHKRNPILTENLTGLARLVRSAVVPAMENVALWHERDISHSSVERGIGPDAT
IHLDFALRRLAGVIERFNIYPDNMAKNLDKLGGLVFSQRVMLALTHKDVSREDAYAAVQGNAMKVWRGEGRFLDFLKADP
VVSKALTETELEELFDLGYHTKNVDVIFQRVFGEQG
>Mature_436_residues
MISRYARPEAAAIWSSQTKYKIWFEIEAHAADAMAELGVIPKLAAETIWEKGRDAVWDSDRIDEIERVTKHDVIAFLTHV
SEIVGPEARFLHQGMTSSDVLDTCFAVQLSRATDLLLEDVDLVLAALKRRALEHKMTVCVGRSHGIHAEPITFGLKLAGY
YAEFQRAKERLAMAKFEIATCAISGAVGTFANVDPRVEQHVADKMGLAVEPVSTQVIPRDRHAAYFAALGVVASSVERLA
TEIRHLQRTEVLEAEEPFDPGQKGSSAMPHKRNPILTENLTGLARLVRSAVVPAMENVALWHERDISHSSVERGIGPDAT
IHLDFALRRLAGVIERFNIYPDNMAKNLDKLGGLVFSQRVMLALTHKDVSREDAYAAVQGNAMKVWRGEGRFLDFLKADP
VVSKALTETELEELFDLGYHTKNVDVIFQRVFGEQG

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=454, Percent_Identity=25.5506607929515, Blast_Score=124, Evalue=1e-28,
Organism=Homo sapiens, GI183227688, Length=385, Percent_Identity=27.012987012987, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1787376, Length=349, Percent_Identity=28.080229226361, Blast_Score=86, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17508577, Length=396, Percent_Identity=27.2727272727273, Blast_Score=114, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI32564234, Length=333, Percent_Identity=28.2282282282282, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323391, Length=395, Percent_Identity=26.8354430379747, Blast_Score=125, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24647570, Length=375, Percent_Identity=26.1333333333333, Blast_Score=91, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48460; Mature: 48460

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISRYARPEAAAIWSSQTKYKIWFEIEAHAADAMAELGVIPKLAAETIWEKGRDAVWDSD
CCCCCCCCCHHHHHCCCCCEEEEEEEECHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCH
RIDEIERVTKHDVIAFLTHVSEIVGPEARFLHQGMTSSDVLDTCFAVQLSRATDLLLEDV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DLVLAALKRRALEHKMTVCVGRSHGIHAEPITFGLKLAGYYAEFQRAKERLAMAKFEIAT
HHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CAISGAVGTFANVDPRVEQHVADKMGLAVEPVSTQVIPRDRHAAYFAALGVVASSVERLA
HHHHCCHHCCCCCCHHHHHHHHHHHCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TEIRHLQRTEVLEAEEPFDPGQKGSSAMPHKRNPILTENLTGLARLVRSAVVPAMENVAL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WHERDISHSSVERGIGPDATIHLDFALRRLAGVIERFNIYPDNMAKNLDKLGGLVFSQRV
HHHCCCCHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
MLALTHKDVSREDAYAAVQGNAMKVWRGEGRFLDFLKADPVVSKALTETELEELFDLGYH
HHHHHHCCCCHHHHHEEECCCEEEEECCCCCEEHHHHCCHHHHHHHHHHHHHHHHHCCCC
TKNVDVIFQRVFGEQG
CCHHHHHHHHHHCCCC
>Mature Secondary Structure
MISRYARPEAAAIWSSQTKYKIWFEIEAHAADAMAELGVIPKLAAETIWEKGRDAVWDSD
CCCCCCCCCHHHHHCCCCCEEEEEEEECHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCH
RIDEIERVTKHDVIAFLTHVSEIVGPEARFLHQGMTSSDVLDTCFAVQLSRATDLLLEDV
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
DLVLAALKRRALEHKMTVCVGRSHGIHAEPITFGLKLAGYYAEFQRAKERLAMAKFEIAT
HHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CAISGAVGTFANVDPRVEQHVADKMGLAVEPVSTQVIPRDRHAAYFAALGVVASSVERLA
HHHHCCHHCCCCCCHHHHHHHHHHHCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
TEIRHLQRTEVLEAEEPFDPGQKGSSAMPHKRNPILTENLTGLARLVRSAVVPAMENVAL
HHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WHERDISHSSVERGIGPDATIHLDFALRRLAGVIERFNIYPDNMAKNLDKLGGLVFSQRV
HHHCCCCHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
MLALTHKDVSREDAYAAVQGNAMKVWRGEGRFLDFLKADPVVSKALTETELEELFDLGYH
HHHHHHCCCCHHHHHEEECCCEEEEECCCCCEEHHHHCCHHHHHHHHHHHHHHHHHCCCC
TKNVDVIFQRVFGEQG
CCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]