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The map label for this gene is mak [H]

Identifier: 16126704

GI number: 16126704

Start: 2671102

End: 2671992

Strand: Direct

Name: mak [H]

Synonym: CC_2465

Alternate gene names: 16126704

Gene position: 2671102-2671992 (Clockwise)

Preceding gene: 16126703

Following gene: 16126705

Centisome position: 66.5

GC content: 71.04

Gene sequence:

>891_bases
ATGATCCGTTTCGGCGTCGACTTTGGTGGCACCAAGATCGAAGTCGCCGCGCTGAACGCGGCCGGCGACTTTGTGGCGCG
CGTGCGCAAGCCCAATCCCGGCAATTACGACGAAGCGCTCGAGGTCGTGGCCGCGCTGGTCGCGGACGCAGAGTCGATGG
CCGGCGGCAGCTGCGCGCGGTTGGGTCTGGGCATTCCGGGTTCGATCTCGCCCCGCACCGGCCTGATCCGCAACGCCAAC
AGCACCTATCTGAACGGCCGCCCGTTCGGCGAGAACCTGGAGACGCGCCTGGCGCGTCCGGTGCGGATCGCCAACGACGC
CAACTGCCTGGCTCTGTCCGAGGCCGCCGACGGCGCGGGCGCGGGCGCCTCCGTCGTCTTCGCCGCCATCGTCGGCACCG
GCTGCGGCGGCGGGGTCGTGGTCGACGGCAAGATCATCAACGGCCACAACGGGATCGGCGGCGAGTGGGGTCACGCCCCA
CTGCCCTGGCCCAAGCCCGAGGAGTATCCGGGTCCGGACTGCTGGTGCGGCCGCAAGGGCTGCCTGGAGACCTGGATCGC
CGGCCCCGCCTTCGCCCGCGACGCCGGCTTCGCCAACGGACAGGCGGCGATGCAGGCGATCGGCGAAGGCGACGTGAAGG
CCAGCGCGGCGCTGGATCGCTATGTCGACCGTCTGGCCCGGTCCCTGGCCGTGGTCTGCGACCTGATCGACCCGGACATC
ATCGTTCTGGGCGGCGGCATGTCGAACGTCGACGCCCTCTACGAGCGTCTGCCCGCCGCCATCGCGCCGCACGTGTTCTC
CGACATCTTCGAGACCCCGGTGCGCAAGGCCGTGCACGGCGACAGCTCGGGCGTGCGCGGCGCCGTCTGGCTCTGGCCGC
CGGAGGTCTAG

Upstream 100 bases:

>100_bases
ATGAGCGCCTGCGCCCCTTCGCCCCGTCCGCGCCCGATCGTCATGCCCGTATCGATGAATGGCCGACCCTGAAGGTCGCC
ATCGAGGAGCGCCTTTTCCC

Downstream 100 bases:

>100_bases
CCGCCACCATGAAGTCCCTGTGCCGCGACTGCGGGTGGACCGGCGCGACCAAGGTCGCGCGGTGTCCCGCCTGCGCCTCG
CCGCGCACAGTGTTCCATGA

Product: ROK family protein

Products: NA

Alternate protein names: D-fructose kinase; Manno(fructo)kinase [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MIRFGVDFGGTKIEVAALNAAGDFVARVRKPNPGNYDEALEVVAALVADAESMAGGSCARLGLGIPGSISPRTGLIRNAN
STYLNGRPFGENLETRLARPVRIANDANCLALSEAADGAGAGASVVFAAIVGTGCGGGVVVDGKIINGHNGIGGEWGHAP
LPWPKPEEYPGPDCWCGRKGCLETWIAGPAFARDAGFANGQAAMQAIGEGDVKASAALDRYVDRLARSLAVVCDLIDPDI
IVLGGGMSNVDALYERLPAAIAPHVFSDIFETPVRKAVHGDSSGVRGAVWLWPPEV

Sequences:

>Translated_296_residues
MIRFGVDFGGTKIEVAALNAAGDFVARVRKPNPGNYDEALEVVAALVADAESMAGGSCARLGLGIPGSISPRTGLIRNAN
STYLNGRPFGENLETRLARPVRIANDANCLALSEAADGAGAGASVVFAAIVGTGCGGGVVVDGKIINGHNGIGGEWGHAP
LPWPKPEEYPGPDCWCGRKGCLETWIAGPAFARDAGFANGQAAMQAIGEGDVKASAALDRYVDRLARSLAVVCDLIDPDI
IVLGGGMSNVDALYERLPAAIAPHVFSDIFETPVRKAVHGDSSGVRGAVWLWPPEV
>Mature_296_residues
MIRFGVDFGGTKIEVAALNAAGDFVARVRKPNPGNYDEALEVVAALVADAESMAGGSCARLGLGIPGSISPRTGLIRNAN
STYLNGRPFGENLETRLARPVRIANDANCLALSEAADGAGAGASVVFAAIVGTGCGGGVVVDGKIINGHNGIGGEWGHAP
LPWPKPEEYPGPDCWCGRKGCLETWIAGPAFARDAGFANGQAAMQAIGEGDVKASAALDRYVDRLARSLAVVCDLIDPDI
IVLGGGMSNVDALYERLPAAIAPHVFSDIFETPVRKAVHGDSSGVRGAVWLWPPEV

Specific function: Catalyzes the phosphorylation of fructose to fructose-6- P. Has also low level glucokinase activity in vitro. Is not able to phosphorylate D-ribose, D-mannitol, D-sorbitol, inositol, and L-threonine [H]

COG id: COG1940

COG function: function code KG; Transcriptional regulator/sugar kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ROK (nagC/xylR) family [H]

Homologues:

Organism=Homo sapiens, GI298566325, Length=272, Percent_Identity=28.3088235294118, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI298566315, Length=272, Percent_Identity=28.3088235294118, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI4885285, Length=272, Percent_Identity=28.3088235294118, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI190014632, Length=272, Percent_Identity=28.3088235294118, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI87081733, Length=303, Percent_Identity=54.1254125412541, Blast_Score=307, Evalue=5e-85,
Organism=Escherichia coli, GI1787363, Length=300, Percent_Identity=36, Blast_Score=174, Evalue=5e-45,
Organism=Escherichia coli, GI1790522, Length=297, Percent_Identity=26.9360269360269, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786891, Length=276, Percent_Identity=25.7246376811594, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1787878, Length=205, Percent_Identity=26.3414634146341, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87082230, Length=261, Percent_Identity=29.5019157088123, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000600 [H]

Pfam domain/function: PF00480 ROK [H]

EC number: =2.7.1.4 [H]

Molecular weight: Translated: 30557; Mature: 30557

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS01125 ROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRFGVDFGGTKIEVAALNAAGDFVARVRKPNPGNYDEALEVVAALVADAESMAGGSCAR
CEEEECCCCCCEEEEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHE
LGLGIPGSISPRTGLIRNANSTYLNGRPFGENLETRLARPVRIANDANCLALSEAADGAG
ECCCCCCCCCCCCCEEECCCCCEECCCCCCCCHHHHHCCCEEECCCCCEEEEECCCCCCC
AGASVVFAAIVGTGCGGGVVVDGKIINGHNGIGGEWGHAPLPWPKPEEYPGPDCWCGRKG
CCHHHHHHHHHHCCCCCCEEEECEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
CLETWIAGPAFARDAGFANGQAAMQAIGEGDVKASAALDRYVDRLARSLAVVCDLIDPDI
HHHHHHCCCCHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCE
IVLGGGMSNVDALYERLPAAIAPHVFSDIFETPVRKAVHGDSSGVRGAVWLWPPEV
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC
>Mature Secondary Structure
MIRFGVDFGGTKIEVAALNAAGDFVARVRKPNPGNYDEALEVVAALVADAESMAGGSCAR
CEEEECCCCCCEEEEEEECCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHE
LGLGIPGSISPRTGLIRNANSTYLNGRPFGENLETRLARPVRIANDANCLALSEAADGAG
ECCCCCCCCCCCCCEEECCCCCEECCCCCCCCHHHHHCCCEEECCCCCEEEEECCCCCCC
AGASVVFAAIVGTGCGGGVVVDGKIINGHNGIGGEWGHAPLPWPKPEEYPGPDCWCGRKG
CCHHHHHHHHHHCCCCCCEEEECEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
CLETWIAGPAFARDAGFANGQAAMQAIGEGDVKASAALDRYVDRLARSLAVVCDLIDPDI
HHHHHHCCCCHHHCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCE
IVLGGGMSNVDALYERLPAAIAPHVFSDIFETPVRKAVHGDSSGVRGAVWLWPPEV
EEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8807285; 9278503; 1744033 [H]