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The map label for this gene is miaA [H]

Identifier: 16126337

GI number: 16126337

Start: 2308289

End: 2309176

Strand: Direct

Name: miaA [H]

Synonym: CC_2098

Alternate gene names: 16126337

Gene position: 2308289-2309176 (Clockwise)

Preceding gene: 16126333

Following gene: 16126338

Centisome position: 57.46

GC content: 69.37

Gene sequence:

>888_bases
GTGGAGAGCTCGGACATCGCGTCCCGCATCTGGCTGATCGCGGGCCCGACCGCAAGCGGCAAGTCCGCCTACGCTCTGGA
TTTGGCCGAACGGATCGACGGCGAGATCGTCAACGCCGACTCCATGCAGATCTATGCGGGCCTCCGCGTCCTCACGGCCG
GTCCCTCGCAAGAGGAAACCGCACGAGCGCCGCATCACCTGTTTCAGATCGTCGACCCGGCCCTCGGCTGGTCGGTCGGA
CGCTGGTTGGAGGCGGCGACCCAGGCGCTCGCCGAGATCCAGGCCCGGGGAAAGCCGGCGATCGTGGTCGGCGGCACCGG
CCTCTATTTCCGCGCCCTGACCCACGGCCTCGCCGACGTGCCGCCGGTGCCCGAAACCCAGCGCGAGATCTCCAGCCTGC
TCTACGCGGCGCGGGGCGAAACCGAGTTCCGCGAGATCCTGAAGCCGCTCGACCCCGAGGCCGAAGCGCGCATTGAAAGC
GGTGACCGCCAGCGTCTGGTCCGGGCCCACGCCGTCGCCATCACCACGGGCAAGTCGCTCACCGCCTGGCAGACCGACAC
CAAGCCGGCCCTCGCCCCAGGATCCTGGAAGGGCCTCGTTCTGGACCCGCCCCGCGCCGAGCTCTACGCGCGCTGTGACG
CCCGTCTGGCGGTCATGGTCGAACAAGGCGCCCTGGACGAGGTTCGCGCGCTGGAAACTCGAGGTCTGGACCCGGCCCTC
CCCGCCCTGAAAGCCGTCGGTTACCGCGAGTTCGCGGCCCATCTTCGGGGTGAGACCTCGCTGGACCAGGCCCTGGACGC
GGCGCGCCAGGAAACCCGTCGCTACGCCAAGCGCCAGTTGACCTGGTTCCGCAATCAAACGCCCGACTGGGAAAGGATCG
TCCCGTGA

Upstream 100 bases:

>100_bases
CAGACCCTCGCGGCGGCTGGACAGGGCCGCTTCGACGCGCGAAACGGCTTGTGCGTAAGCGTCGGGGTTTGCCCCCACGA
CGGTGAGGACGGTCGGAATG

Downstream 100 bases:

>100_bases
CCCTGATCGAAGAGGTCGCCGCGCTGTTGAAGGCGCGGGGCGAAACCATCGCCGTGTCCGAGTCCTCCACCGGCGGATTG
ATCTCGGCCGCCCTGCTGAG

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MESSDIASRIWLIAGPTASGKSAYALDLAERIDGEIVNADSMQIYAGLRVLTAGPSQEETARAPHHLFQIVDPALGWSVG
RWLEAATQALAEIQARGKPAIVVGGTGLYFRALTHGLADVPPVPETQREISSLLYAARGETEFREILKPLDPEAEARIES
GDRQRLVRAHAVAITTGKSLTAWQTDTKPALAPGSWKGLVLDPPRAELYARCDARLAVMVEQGALDEVRALETRGLDPAL
PALKAVGYREFAAHLRGETSLDQALDAARQETRRYAKRQLTWFRNQTPDWERIVP

Sequences:

>Translated_295_residues
MESSDIASRIWLIAGPTASGKSAYALDLAERIDGEIVNADSMQIYAGLRVLTAGPSQEETARAPHHLFQIVDPALGWSVG
RWLEAATQALAEIQARGKPAIVVGGTGLYFRALTHGLADVPPVPETQREISSLLYAARGETEFREILKPLDPEAEARIES
GDRQRLVRAHAVAITTGKSLTAWQTDTKPALAPGSWKGLVLDPPRAELYARCDARLAVMVEQGALDEVRALETRGLDPAL
PALKAVGYREFAAHLRGETSLDQALDAARQETRRYAKRQLTWFRNQTPDWERIVP
>Mature_295_residues
MESSDIASRIWLIAGPTASGKSAYALDLAERIDGEIVNADSMQIYAGLRVLTAGPSQEETARAPHHLFQIVDPALGWSVG
RWLEAATQALAEIQARGKPAIVVGGTGLYFRALTHGLADVPPVPETQREISSLLYAARGETEFREILKPLDPEAEARIES
GDRQRLVRAHAVAITTGKSLTAWQTDTKPALAPGSWKGLVLDPPRAELYARCDARLAVMVEQGALDEVRALETRGLDPAL
PALKAVGYREFAAHLRGETSLDQALDAARQETRRYAKRQLTWFRNQTPDWERIVP

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=306, Percent_Identity=30.0653594771242, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1790613, Length=284, Percent_Identity=37.6760563380282, Blast_Score=162, Evalue=3e-41,
Organism=Caenorhabditis elegans, GI25144712, Length=300, Percent_Identity=23, Blast_Score=78, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6324848, Length=293, Percent_Identity=27.6450511945392, Blast_Score=125, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24649830, Length=333, Percent_Identity=27.3273273273273, Blast_Score=115, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 32305; Mature: 32305

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESSDIASRIWLIAGPTASGKSAYALDLAERIDGEIVNADSMQIYAGLRVLTAGPSQEET
CCCCHHHHEEEEEECCCCCCCCHHHHHHHHHCCCCEECCCCEEEEECEEEEECCCCHHHH
ARAPHHLFQIVDPALGWSVGRWLEAATQALAEIQARGKPAIVVGGTGLYFRALTHGLADV
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCC
PPVPETQREISSLLYAARGETEFREILKPLDPEAEARIESGDRQRLVRAHAVAITTGKSL
CCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHEEEECCCCE
TAWQTDTKPALAPGSWKGLVLDPPRAELYARCDARLAVMVEQGALDEVRALETRGLDPAL
EEECCCCCCCCCCCCCCCEEECCCHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCHHH
PALKAVGYREFAAHLRGETSLDQALDAARQETRRYAKRQLTWFRNQTPDWERIVP
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCC
>Mature Secondary Structure
MESSDIASRIWLIAGPTASGKSAYALDLAERIDGEIVNADSMQIYAGLRVLTAGPSQEET
CCCCHHHHEEEEEECCCCCCCCHHHHHHHHHCCCCEECCCCEEEEECEEEEECCCCHHHH
ARAPHHLFQIVDPALGWSVGRWLEAATQALAEIQARGKPAIVVGGTGLYFRALTHGLADV
HHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHCCC
PPVPETQREISSLLYAARGETEFREILKPLDPEAEARIESGDRQRLVRAHAVAITTGKSL
CCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHCCCHHHHHHHHHHEEEECCCCE
TAWQTDTKPALAPGSWKGLVLDPPRAELYARCDARLAVMVEQGALDEVRALETRGLDPAL
EEECCCCCCCCCCCCCCCEEECCCHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCCHHH
PALKAVGYREFAAHLRGETSLDQALDAARQETRRYAKRQLTWFRNQTPDWERIVP
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA