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The map label for this gene is glnA [H]

Identifier: 16126212

GI number: 16126212

Start: 2166922

End: 2168331

Strand: Direct

Name: glnA [H]

Synonym: CC_1969

Alternate gene names: 16126212

Gene position: 2166922-2168331 (Clockwise)

Preceding gene: 16126211

Following gene: 16126213

Centisome position: 53.94

GC content: 62.77

Gene sequence:

>1410_bases
ATGAGCACCGCCAAGGACATCCTGAACCTGATCAAGGAAAAGGACGTGAAGTACGTCGACGTCCGTTTCACGGACGTGCG
CGGCAAGATGCAGCACGTGACCTTCGACATCGACCTCGTCGACGACGACTTCCTGAACGACGGCACCATGTTCGACGGTT
CGTCGATCGCCGGCTGGAAGGCCATCAACGAGTCGGACATGAAGCTGCGCCCCGACCTGTCGAGCGCGATCATCGACCCG
TTCTACCAGCAGACCACGCTGGCGCTGTTCTGCGACGTCGTGAACCCCGACACCGGCACGCCCTACAACCGCGACCCGCG
TTCGATCGCCAAGGCCGCGCTGAACTACCTGAAGTCGGCCGGCGTGGGCGACACCGTCTACTTCGGCCCTGAAGCCGAGT
TCTTCATCTTCGACGACGTGCGCTGGTCGACCGCCCCGCACGACACCAGCTACTCGTTCGATTCGACCGAACTGCCGGTC
AACTCGAACAAGCAGTACGCCGAAGGCAACATGGGCCACCGCCCGGGCCCGAAGGGCGGCTACTTCCCGGTGAACCCGGT
CGACAGCTGCCAAGACCTGCGCGGCGAAATGCTGGCCGTCATGGGCGAGCTGGGCATGAAGCCGGAAAAGCACCACCACG
AAGTGGCGCCGGCCCAGCACGAGCTTGGCCTGAAGTTCGACACCATGGTCACCATGGCCGACCGGATGCAGCTCTATAAG
TACGTCATCCACAACGTCGCGGCCGCCTACGGCAAGACCGCCACCTTCATGGCCAAGCCGATGTTCGCCGACAACGGTTC
GGGCATGCACGTGCACCAGTCGATCTGGCAGGACGGCAAGCCGCTGTTCGCCGGCGACAAGTATGCTGGCCTGTCGCAGG
AATGCCTGTGGTACATCGGCGGCATCATCAAGCACGCCAAGGCCATCAACGCGTTCTCGAACTCGACGACGAACTCGTAC
AAGCGCCTGGTGCCCGGCTACGAAGCCCCGGTGAAGCTGGCCTACAGCTCGCGCAACCGCTCGGCCTCGATCCGCATCCC
GCACGTCGACTCGCCGAAGGCTAAGCGTCTGGAAGCCCGCTTCCCCGACCCGATGGGCAACCCCTACCTGACGTTCGTCG
CTCTGCTGATGGCCGGCCTGGACGGCATCATCAACAAGATCGATCCGGGCGCTCCGGCCGACAAGAACCTGTACGATCTG
CCGCCCCGCGAGCAGAAGAAGATCCCGGAAGTCTGCGGCTCGCTGCGCGAAGCCCTGGAAAACCTCGACAAGGACCGCGC
CTTCCTGAAGGCCGGCGGTGTCATGGATGACGACTTCATCGACAGCTACATCGAGCTGAAGATGGAAGAGGTGATGCGCC
TGCAACTGCACCCGCACCCGGTCGAATTCGAGATGTACTACAAGTGCTAA

Upstream 100 bases:

>100_bases
GAGATCGCGGAAGTGATCCGCATCCGAACCGGAGAAAACGGTCCGGCCGCCGTCTAGGCCGGTTCGTCAGCAACTAAAGA
GTTCTACAAGGGGATACGGA

Downstream 100 bases:

>100_bases
GCGCTTAAGGCTTAGGACAAACGCCAAGGGGCCGGAGAGCGATCTCCGGCCCCTTTTCATTGCGCCGCGCCAGGACGGCG
CTCCAGAAGTAGAACGACTT

Product: glutamine synthetase, class I

Products: NA

Alternate protein names: Glutamate--ammonia ligase I; Glutamine synthetase I; GSI [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSTAKDILNLIKEKDVKYVDVRFTDVRGKMQHVTFDIDLVDDDFLNDGTMFDGSSIAGWKAINESDMKLRPDLSSAIIDP
FYQQTTLALFCDVVNPDTGTPYNRDPRSIAKAALNYLKSAGVGDTVYFGPEAEFFIFDDVRWSTAPHDTSYSFDSTELPV
NSNKQYAEGNMGHRPGPKGGYFPVNPVDSCQDLRGEMLAVMGELGMKPEKHHHEVAPAQHELGLKFDTMVTMADRMQLYK
YVIHNVAAAYGKTATFMAKPMFADNGSGMHVHQSIWQDGKPLFAGDKYAGLSQECLWYIGGIIKHAKAINAFSNSTTNSY
KRLVPGYEAPVKLAYSSRNRSASIRIPHVDSPKAKRLEARFPDPMGNPYLTFVALLMAGLDGIINKIDPGAPADKNLYDL
PPREQKKIPEVCGSLREALENLDKDRAFLKAGGVMDDDFIDSYIELKMEEVMRLQLHPHPVEFEMYYKC

Sequences:

>Translated_469_residues
MSTAKDILNLIKEKDVKYVDVRFTDVRGKMQHVTFDIDLVDDDFLNDGTMFDGSSIAGWKAINESDMKLRPDLSSAIIDP
FYQQTTLALFCDVVNPDTGTPYNRDPRSIAKAALNYLKSAGVGDTVYFGPEAEFFIFDDVRWSTAPHDTSYSFDSTELPV
NSNKQYAEGNMGHRPGPKGGYFPVNPVDSCQDLRGEMLAVMGELGMKPEKHHHEVAPAQHELGLKFDTMVTMADRMQLYK
YVIHNVAAAYGKTATFMAKPMFADNGSGMHVHQSIWQDGKPLFAGDKYAGLSQECLWYIGGIIKHAKAINAFSNSTTNSY
KRLVPGYEAPVKLAYSSRNRSASIRIPHVDSPKAKRLEARFPDPMGNPYLTFVALLMAGLDGIINKIDPGAPADKNLYDL
PPREQKKIPEVCGSLREALENLDKDRAFLKAGGVMDDDFIDSYIELKMEEVMRLQLHPHPVEFEMYYKC
>Mature_468_residues
STAKDILNLIKEKDVKYVDVRFTDVRGKMQHVTFDIDLVDDDFLNDGTMFDGSSIAGWKAINESDMKLRPDLSSAIIDPF
YQQTTLALFCDVVNPDTGTPYNRDPRSIAKAALNYLKSAGVGDTVYFGPEAEFFIFDDVRWSTAPHDTSYSFDSTELPVN
SNKQYAEGNMGHRPGPKGGYFPVNPVDSCQDLRGEMLAVMGELGMKPEKHHHEVAPAQHELGLKFDTMVTMADRMQLYKY
VIHNVAAAYGKTATFMAKPMFADNGSGMHVHQSIWQDGKPLFAGDKYAGLSQECLWYIGGIIKHAKAINAFSNSTTNSYK
RLVPGYEAPVKLAYSSRNRSASIRIPHVDSPKAKRLEARFPDPMGNPYLTFVALLMAGLDGIINKIDPGAPADKNLYDLP
PREQKKIPEVCGSLREALENLDKDRAFLKAGGVMDDDFIDSYIELKMEEVMRLQLHPHPVEFEMYYKC

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=468, Percent_Identity=62.3931623931624, Blast_Score=612, Evalue=1e-176,
Organism=Escherichia coli, GI87081870, Length=396, Percent_Identity=23.7373737373737, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52485; Mature: 52354

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTAKDILNLIKEKDVKYVDVRFTDVRGKMQHVTFDIDLVDDDFLNDGTMFDGSSIAGWK
CCCHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEEEEECCCCCCCCCCEECCCCCCCCC
AINESDMKLRPDLSSAIIDPFYQQTTLALFCDVVNPDTGTPYNRDPRSIAKAALNYLKSA
CCCCCCCEECCCHHHHHHHHHHHHHEEHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHC
GVGDTVYFGPEAEFFIFDDVRWSTAPHDTSYSFDSTELPVNSNKQYAEGNMGHRPGPKGG
CCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
YFPVNPVDSCQDLRGEMLAVMGELGMKPEKHHHEVAPAQHELGLKFDTMVTMADRMQLYK
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHH
YVIHNVAAAYGKTATFMAKPMFADNGSGMHVHQSIWQDGKPLFAGDKYAGLSQECLWYIG
HHHHHHHHHHCCCHHEEECCEECCCCCCCCHHHHHHHCCCCEECCCCCCCCCHHHHHHHH
GIIKHAKAINAFSNSTTNSYKRLVPGYEAPVKLAYSSRNRSASIRIPHVDSPKAKRLEAR
HHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEECCCCCCEEEECCCCCCCHHHHHHCC
FPDPMGNPYLTFVALLMAGLDGIINKIDPGAPADKNLYDLPPREQKKIPEVCGSLREALE
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
NLDKDRAFLKAGGVMDDDFIDSYIELKMEEVMRLQLHPHPVEFEMYYKC
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC
>Mature Secondary Structure 
STAKDILNLIKEKDVKYVDVRFTDVRGKMQHVTFDIDLVDDDFLNDGTMFDGSSIAGWK
CCHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEEEEEECCCCCCCCCCEECCCCCCCCC
AINESDMKLRPDLSSAIIDPFYQQTTLALFCDVVNPDTGTPYNRDPRSIAKAALNYLKSA
CCCCCCCEECCCHHHHHHHHHHHHHEEHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHC
GVGDTVYFGPEAEFFIFDDVRWSTAPHDTSYSFDSTELPVNSNKQYAEGNMGHRPGPKGG
CCCCEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
YFPVNPVDSCQDLRGEMLAVMGELGMKPEKHHHEVAPAQHELGLKFDTMVTMADRMQLYK
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCHHHCCCCHHHHHHHHHHHHHHH
YVIHNVAAAYGKTATFMAKPMFADNGSGMHVHQSIWQDGKPLFAGDKYAGLSQECLWYIG
HHHHHHHHHHCCCHHEEECCEECCCCCCCCHHHHHHHCCCCEECCCCCCCCCHHHHHHHH
GIIKHAKAINAFSNSTTNSYKRLVPGYEAPVKLAYSSRNRSASIRIPHVDSPKAKRLEAR
HHHHHHHHHHHHCCCCCHHHHHHCCCCCCCEEEEECCCCCCEEEECCCCCCCHHHHHHCC
FPDPMGNPYLTFVALLMAGLDGIINKIDPGAPADKNLYDLPPREQKKIPEVCGSLREALE
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
NLDKDRAFLKAGGVMDDDFIDSYIELKMEEVMRLQLHPHPVEFEMYYKC
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275; 2859270; 2793830 [H]