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The map label for this gene is mls [H]

Identifier: 16126009

GI number: 16126009

Start: 1948475

End: 1950052

Strand: Direct

Name: mls [H]

Synonym: CC_1765

Alternate gene names: 16126009

Gene position: 1948475-1950052 (Clockwise)

Preceding gene: 16126008

Following gene: 16126018

Centisome position: 48.51

GC content: 65.91

Gene sequence:

>1578_bases
ATGCCGCTCGACATCGCCGCCAACATCCAGGTCACCGCTCCCGTTGAAGGCCGAGCTGCGGAAGTGCTCACCCCCGAAGC
CCTGGCCTTCGTCGCCGATCTGCACCGTCGGTTCGACGCGCGTCGCCGCCAGTTGCTGGAAGCCCGTCAGGCGCGCCAGG
CGCGCTTTGACGCCGGCGAACTGCCCGACTTCCTGGACGAGACCGCATCGATCCGCAGCGCCGACTGGACGATCGCAGCC
ATTCCCAGCGACCTGCTCGATCGCCGGGTCGAAATCACCGGGCCGGTCAACCGCAAGATGATCATCAACGCCTTGAACTG
CGGGGCCAAGGTGTTCATGGCCGACTTCGAGGACGCCACGGCCCCGACCTGGGCCAACGTCGTCGAAGGCCAGATCAATC
TGAAAGACCGCTGGCAAGGCGAGCTGACCCACGTCGACGCGAAGTCGGGCAAGGCCTACGCCATGGGGCCAACCTCCGCT
GTGCTGAAAATCCGCCCGCGCGGCTGGCACCTGATGGAGCGCCACCTAGAGGTCGATGGCCAGGCCGTGGCCGGGGCCCT
GTTCGACTTTGGCCTCTACGCGTTCCACAACGCCAAGGCCGCCATCGCCAAAGGCTCAGGGCCTTACTTCTACCTGCCCA
AGCTCGAGAGCCACCTGGAAGCACGCCTGTGGAATGACGTCTTCATCCGCGCCCAGGAGGTGCTCGACCTGCCGGTGGGA
ACGATCAAGGCCACGGTGCTGATTGAGACCATCCCCGCCGCCTTCGAGATGGACGAGATCCTGTTCGAGCTGAAGGACCA
CATCGTGGGCCTGAACTGCGGCCGCTGGGACTACATCTTCTCGTTCATCAAGCGCCTGGGCCGTCGCGAAGCGTTCCTGA
CGCCGGACCGCTCGGCCATGGTGATGGGCAAGGCCTTCCTGGGCGCCTATTCGCTGAAGCTGATCCAGACCTGTCACCGC
CGTGGCGCGTTCGCGATGGGCGGCATGGCGGCCCAGATCCCGGTCAAGGGCGACGAGGCCGCCAACAACGCGGCCTTCGC
CAAGGTCCGGGCAGACAAGGAGCGGGAAGCCGGAGCCGGCCACGATGGCACCTGGGTGGCGCACCCCGACCTCGTTCCGG
TGGCCATGGAGGTGTTCAACCGCCTGATGCCGACGCCGAACCAGCTCGACCGCAAGCTCGACGATCTCTCGATCACTCAG
GCCCAGATGCTGGAGTTGCACGAGGGTTCGCGCACCGAGGCTGGCGTCCGCGAAAACATTCGGGTGGGCGTCCGCTACAC
CCAGGCCTGGATCGAAGGTCGCGGCGCGGTGCCGCTCTACAACCTGATGGAGGACGCGGCGACCGCAGAGATCTGCCGCA
CCCAGCTCTGGCAGTGGGTGCGCCTCGGCGCCGCACTCGACGACGGCCGCGTGCTGAGCTCAGAACTGTTCGTCAGCCTG
TTCAGCCAGGAGATGAGCGACCTGCGCCGCGAGTTCGCCTCCCCTCGCCTGGAGGAAGCCGCGCACATCTTCACCAGGAT
GGTGCTCTCGGACGATCTGGAGGAGTTCCTGACGATTCCTGCGTACGAGTTGCTCTAA

Upstream 100 bases:

>100_bases
GCGCCGAAAAGAGCGCCTAGCCATCAAGAACCGACCAGAGTCCCGATAACGGCTCCCCGGATATCCGGGGAGCCGATCCC
ACCGCCACGGAAAGCTTCCC

Downstream 100 bases:

>100_bases
CTTCGCCGCATCGTGATCGGTGAGTTCGCCGATCACGATGCCGGCGCCAGACACATGCGCCAAAAGCTGCCGTTGGTGCG
GCTCCAGGAATGCGACATTC

Product: malate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 525; Mature: 524

Protein sequence:

>525_residues
MPLDIAANIQVTAPVEGRAAEVLTPEALAFVADLHRRFDARRRQLLEARQARQARFDAGELPDFLDETASIRSADWTIAA
IPSDLLDRRVEITGPVNRKMIINALNCGAKVFMADFEDATAPTWANVVEGQINLKDRWQGELTHVDAKSGKAYAMGPTSA
VLKIRPRGWHLMERHLEVDGQAVAGALFDFGLYAFHNAKAAIAKGSGPYFYLPKLESHLEARLWNDVFIRAQEVLDLPVG
TIKATVLIETIPAAFEMDEILFELKDHIVGLNCGRWDYIFSFIKRLGRREAFLTPDRSAMVMGKAFLGAYSLKLIQTCHR
RGAFAMGGMAAQIPVKGDEAANNAAFAKVRADKEREAGAGHDGTWVAHPDLVPVAMEVFNRLMPTPNQLDRKLDDLSITQ
AQMLELHEGSRTEAGVRENIRVGVRYTQAWIEGRGAVPLYNLMEDAATAEICRTQLWQWVRLGAALDDGRVLSSELFVSL
FSQEMSDLRREFASPRLEEAAHIFTRMVLSDDLEEFLTIPAYELL

Sequences:

>Translated_525_residues
MPLDIAANIQVTAPVEGRAAEVLTPEALAFVADLHRRFDARRRQLLEARQARQARFDAGELPDFLDETASIRSADWTIAA
IPSDLLDRRVEITGPVNRKMIINALNCGAKVFMADFEDATAPTWANVVEGQINLKDRWQGELTHVDAKSGKAYAMGPTSA
VLKIRPRGWHLMERHLEVDGQAVAGALFDFGLYAFHNAKAAIAKGSGPYFYLPKLESHLEARLWNDVFIRAQEVLDLPVG
TIKATVLIETIPAAFEMDEILFELKDHIVGLNCGRWDYIFSFIKRLGRREAFLTPDRSAMVMGKAFLGAYSLKLIQTCHR
RGAFAMGGMAAQIPVKGDEAANNAAFAKVRADKEREAGAGHDGTWVAHPDLVPVAMEVFNRLMPTPNQLDRKLDDLSITQ
AQMLELHEGSRTEAGVRENIRVGVRYTQAWIEGRGAVPLYNLMEDAATAEICRTQLWQWVRLGAALDDGRVLSSELFVSL
FSQEMSDLRREFASPRLEEAAHIFTRMVLSDDLEEFLTIPAYELL
>Mature_524_residues
PLDIAANIQVTAPVEGRAAEVLTPEALAFVADLHRRFDARRRQLLEARQARQARFDAGELPDFLDETASIRSADWTIAAI
PSDLLDRRVEITGPVNRKMIINALNCGAKVFMADFEDATAPTWANVVEGQINLKDRWQGELTHVDAKSGKAYAMGPTSAV
LKIRPRGWHLMERHLEVDGQAVAGALFDFGLYAFHNAKAAIAKGSGPYFYLPKLESHLEARLWNDVFIRAQEVLDLPVGT
IKATVLIETIPAAFEMDEILFELKDHIVGLNCGRWDYIFSFIKRLGRREAFLTPDRSAMVMGKAFLGAYSLKLIQTCHRR
GAFAMGGMAAQIPVKGDEAANNAAFAKVRADKEREAGAGHDGTWVAHPDLVPVAMEVFNRLMPTPNQLDRKLDDLSITQA
QMLELHEGSRTEAGVRENIRVGVRYTQAWIEGRGAVPLYNLMEDAATAEICRTQLWQWVRLGAALDDGRVLSSELFVSLF
SQEMSDLRREFASPRLEEAAHIFTRMVLSDDLEEFLTIPAYELL

Specific function: Glyoxylate bypass; second step. [C]

COG id: COG2225

COG function: function code C; Malate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the malate synthase family [H]

Homologues:

Organism=Escherichia coli, GI1790444, Length=521, Percent_Identity=53.9347408829175, Blast_Score=552, Evalue=1e-158,
Organism=Caenorhabditis elegans, GI17561814, Length=509, Percent_Identity=52.8487229862475, Blast_Score=543, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI71982926, Length=404, Percent_Identity=55.6930693069307, Blast_Score=462, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6324212, Length=523, Percent_Identity=47.4187380497132, Blast_Score=488, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6322222, Length=524, Percent_Identity=45.8015267175573, Blast_Score=471, Evalue=1e-134,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011076
- InterPro:   IPR006252
- InterPro:   IPR001465
- InterPro:   IPR019830 [H]

Pfam domain/function: PF01274 Malate_synthase [H]

EC number: =2.3.3.9 [H]

Molecular weight: Translated: 58545; Mature: 58414

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: PS00510 MALATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLDIAANIQVTAPVEGRAAEVLTPEALAFVADLHRRFDARRRQLLEARQARQARFDAGE
CCCEEEECEEEECCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LPDFLDETASIRSADWTIAAIPSDLLDRRVEITGPVNRKMIINALNCGAKVFMADFEDAT
CCHHHHHHHCCCCCCEEEEECCHHHHHCEEEECCCCCCCCEEEECCCCCEEEEECCCCCC
APTWANVVEGQINLKDRWQGELTHVDAKSGKAYAMGPTSAVLKIRPRGWHLMERHLEVDG
CCCHHHHHCCCCCCCCCCCCCEEEEECCCCCEEEECCCCEEEEECCCCHHHHHHHHCCCC
QAVAGALFDFGLYAFHNAKAAIAKGSGPYFYLPKLESHLEARLWNDVFIRAQEVLDLPVG
HHHHHHHHHHHHHHHHCCCEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCC
TIKATVLIETIPAAFEMDEILFELKDHIVGLNCGRWDYIFSFIKRLGRREAFLTPDRSAM
HHHHEEEEECCCCHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCCCCCCCCCCHH
VMGKAFLGAYSLKLIQTCHRRGAFAMGGMAAQIPVKGDEAANNAAFAKVRADKEREAGAG
HHHHHHHHHHHHHHHHHHHHCCCEEECCEEEECCCCCCCCCCCCCEEEEECCCHHCCCCC
HDGTWVAHPDLVPVAMEVFNRLMPTPNQLDRKLDDLSITQAQMLELHEGSRTEAGVRENI
CCCCEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCC
RVGVRYTQAWIEGRGAVPLYNLMEDAATAEICRTQLWQWVRLGAALDDGRVLSSELFVSL
EEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
FSQEMSDLRREFASPRLEEAAHIFTRMVLSDDLEEFLTIPAYELL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCC
>Mature Secondary Structure 
PLDIAANIQVTAPVEGRAAEVLTPEALAFVADLHRRFDARRRQLLEARQARQARFDAGE
CCEEEECEEEECCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LPDFLDETASIRSADWTIAAIPSDLLDRRVEITGPVNRKMIINALNCGAKVFMADFEDAT
CCHHHHHHHCCCCCCEEEEECCHHHHHCEEEECCCCCCCCEEEECCCCCEEEEECCCCCC
APTWANVVEGQINLKDRWQGELTHVDAKSGKAYAMGPTSAVLKIRPRGWHLMERHLEVDG
CCCHHHHHCCCCCCCCCCCCCEEEEECCCCCEEEECCCCEEEEECCCCHHHHHHHHCCCC
QAVAGALFDFGLYAFHNAKAAIAKGSGPYFYLPKLESHLEARLWNDVFIRAQEVLDLPVG
HHHHHHHHHHHHHHHHCCCEEEECCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCC
TIKATVLIETIPAAFEMDEILFELKDHIVGLNCGRWDYIFSFIKRLGRREAFLTPDRSAM
HHHHEEEEECCCCHHHHHHHHHHHHHHHEECCCCCHHHHHHHHHHHCCCCCCCCCCCCHH
VMGKAFLGAYSLKLIQTCHRRGAFAMGGMAAQIPVKGDEAANNAAFAKVRADKEREAGAG
HHHHHHHHHHHHHHHHHHHHCCCEEECCEEEECCCCCCCCCCCCCEEEEECCCHHCCCCC
HDGTWVAHPDLVPVAMEVFNRLMPTPNQLDRKLDDLSITQAQMLELHEGSRTEAGVRENI
CCCCEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCCCCC
RVGVRYTQAWIEGRGAVPLYNLMEDAATAEICRTQLWQWVRLGAALDDGRVLSSELFVSL
EEEEEEEEHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
FSQEMSDLRREFASPRLEEAAHIFTRMVLSDDLEEFLTIPAYELL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA