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The map label for this gene is ntrX [H]

Identifier: 16125987

GI number: 16125987

Start: 1917576

End: 1918964

Strand: Direct

Name: ntrX [H]

Synonym: CC_1743

Alternate gene names: 16125987

Gene position: 1917576-1918964 (Clockwise)

Preceding gene: 16125986

Following gene: 16125988

Centisome position: 47.74

GC content: 67.31

Gene sequence:

>1389_bases
ATGAGCGCCGACGTTCTTGTGGTGGATGACGAGGCCGACATTCGGGATCTCGTCGCCGGCATCCTGGAGGATGAAGGCTA
TGCTGTGCGCACGGCCGCCGACTCTGACCAAGCCCTGGCCGCGATCCGGGCGCGCAAGCCCGCTTTGCTGGTGCTGGATA
TCTGGATGCAGGGCGGCGGCATGGACGGGCTTGAGCTGCTCGACATGGTCAAGGCCTTGGACGCCGACCTGCCGGTCATC
ATGATCTCGGGCCACGGCAACATCGAGACGGCCGTAAGCGCCATAAAGCGCGGCGCGTACGAGTTCCTCGAAAAGCCGTT
CAAGTCGGACCGGCTTTTGCTGATCGTCGAGCGCGCCCTTGAGGCGGCGGGGCTTCGCCGGGAGAACCGTCGGCTTCGTG
CGCAGACCCTGTCTCCGGACGGCCTGATCGGCAAATCGGCCCCGGCCCAGGCTCTGCGGCAGCTGATCCTGAAGGTGGCC
CCCGCCAACAGCCGCGTTCTGGTCTCCGGTCCGCCGGGGGCGGGTAAGGAACTCGTCGCGCGACTGATCCATGGGGCGAG
CACGCGCGCCCGTCAGGAGTTTGTCGCGGTCAGCGCCGCCGGCATGGCGCCCGAACGCCTGGACGTCGAGTTGTTTGGCG
AGGAAGGCGAGGGCGGCCGGCCCCGCAAGATCGGCGTGTTCGAGCGGGCTCACGGGGGGACGTTGTATCTCGACGAGGTC
GCCGACATGCCGCGCGAGACCCAGGGCCGTATCTTGCGGGTTCTGGTGGAGCAGCGCTTCCGCCGGGTGGGCGGCGACAA
TGACGTCCAGGTGGATGTGCGCGTGATTTCGTCCAGTTCGCGTGACTTGCGTGACGAGATCGCCGCCGGGCGGTTCCGTG
AGGATCTCTTCCACAGGCTGAACGTCGTGCCAGTGCGCGTTCCGGGTCTGGCCGAGCGGCGTGAGGACATTCCGGAACTG
ATCAACTACTTCGTCGAGCGCATCAGCGAGGCGACGGGTCTGGCGCGCCGGCGCCTCGGTGAGGATGCTCTGGCGACGCT
TCAGGTCCAAGCATGGCCCGGCAATGTTCGCCAGCTTCGCAACAACGTCGAGCGCATGCTGATCCTGGCGTCGGGCGAGC
CCGGCGACGTGATCACGGCCGAAATGCTGTCAGGCGCCGAGCAGCCCAGCGCGGGCAACGCCGGCGCGATCGGGGCGGAG
CGGATCATCGCCCTGCCGCTGCGCGAGGCGCGCGAGCTGTTCGAGCGCGAGTACCTCAACGCCCAGATCCTGAGGTTCGG
CGGCAATATCTCGCGGACGGCCAACTTCATCGGCATGGAGCGGTCCGCCCTGCACCGTAAGCTGAAGTCCCTGGGCGTTT
CCGGCGCTCGGGGCGATGAGGAAGAGTAG

Upstream 100 bases:

>100_bases
TCGTCGATGCGCGCGAGCCGCCTGGCGCGCGCGTCGTGATGAAGTTTCCAACGACCGCGCGTCTGCCCGTCGCGGCCCAG
AGTGGCGTTGAGGAGATGAT

Downstream 100 bases:

>100_bases
ATGTCGCGTTTCGCCTATGTGAATGGCCGGTTTGTTCGCCACGGCGAGGCGGCGGTGCATATCGAGGATCGCGGCTATCA
GCTAGCCGACGGCGTGTACG

Product: nitrogen regulation protein NtrX

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 462; Mature: 461

Protein sequence:

>462_residues
MSADVLVVDDEADIRDLVAGILEDEGYAVRTAADSDQALAAIRARKPALLVLDIWMQGGGMDGLELLDMVKALDADLPVI
MISGHGNIETAVSAIKRGAYEFLEKPFKSDRLLLIVERALEAAGLRRENRRLRAQTLSPDGLIGKSAPAQALRQLILKVA
PANSRVLVSGPPGAGKELVARLIHGASTRARQEFVAVSAAGMAPERLDVELFGEEGEGGRPRKIGVFERAHGGTLYLDEV
ADMPRETQGRILRVLVEQRFRRVGGDNDVQVDVRVISSSSRDLRDEIAAGRFREDLFHRLNVVPVRVPGLAERREDIPEL
INYFVERISEATGLARRRLGEDALATLQVQAWPGNVRQLRNNVERMLILASGEPGDVITAEMLSGAEQPSAGNAGAIGAE
RIIALPLREARELFEREYLNAQILRFGGNISRTANFIGMERSALHRKLKSLGVSGARGDEEE

Sequences:

>Translated_462_residues
MSADVLVVDDEADIRDLVAGILEDEGYAVRTAADSDQALAAIRARKPALLVLDIWMQGGGMDGLELLDMVKALDADLPVI
MISGHGNIETAVSAIKRGAYEFLEKPFKSDRLLLIVERALEAAGLRRENRRLRAQTLSPDGLIGKSAPAQALRQLILKVA
PANSRVLVSGPPGAGKELVARLIHGASTRARQEFVAVSAAGMAPERLDVELFGEEGEGGRPRKIGVFERAHGGTLYLDEV
ADMPRETQGRILRVLVEQRFRRVGGDNDVQVDVRVISSSSRDLRDEIAAGRFREDLFHRLNVVPVRVPGLAERREDIPEL
INYFVERISEATGLARRRLGEDALATLQVQAWPGNVRQLRNNVERMLILASGEPGDVITAEMLSGAEQPSAGNAGAIGAE
RIIALPLREARELFEREYLNAQILRFGGNISRTANFIGMERSALHRKLKSLGVSGARGDEEE
>Mature_461_residues
SADVLVVDDEADIRDLVAGILEDEGYAVRTAADSDQALAAIRARKPALLVLDIWMQGGGMDGLELLDMVKALDADLPVIM
ISGHGNIETAVSAIKRGAYEFLEKPFKSDRLLLIVERALEAAGLRRENRRLRAQTLSPDGLIGKSAPAQALRQLILKVAP
ANSRVLVSGPPGAGKELVARLIHGASTRARQEFVAVSAAGMAPERLDVELFGEEGEGGRPRKIGVFERAHGGTLYLDEVA
DMPRETQGRILRVLVEQRFRRVGGDNDVQVDVRVISSSSRDLRDEIAAGRFREDLFHRLNVVPVRVPGLAERREDIPELI
NYFVERISEATGLARRRLGEDALATLQVQAWPGNVRQLRNNVERMLILASGEPGDVITAEMLSGAEQPSAGNAGAIGAER
IIALPLREARELFEREYLNAQILRFGGNISRTANFIGMERSALHRKLKSLGVSGARGDEEE

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=466, Percent_Identity=36.9098712446352, Blast_Score=280, Evalue=2e-76,
Organism=Escherichia coli, GI1788550, Length=459, Percent_Identity=35.5119825708061, Blast_Score=274, Evalue=1e-74,
Organism=Escherichia coli, GI1790437, Length=453, Percent_Identity=37.5275938189845, Blast_Score=267, Evalue=9e-73,
Organism=Escherichia coli, GI1788905, Length=451, Percent_Identity=34.589800443459, Blast_Score=246, Evalue=2e-66,
Organism=Escherichia coli, GI87082152, Length=316, Percent_Identity=37.9746835443038, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1789087, Length=319, Percent_Identity=36.3636363636364, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI87082117, Length=342, Percent_Identity=35.0877192982456, Blast_Score=177, Evalue=9e-46,
Organism=Escherichia coli, GI1787583, Length=306, Percent_Identity=35.2941176470588, Blast_Score=177, Evalue=2e-45,
Organism=Escherichia coli, GI1786524, Length=328, Percent_Identity=37.5, Blast_Score=176, Evalue=2e-45,
Organism=Escherichia coli, GI1789233, Length=240, Percent_Identity=38.75, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI87081872, Length=317, Percent_Identity=33.1230283911672, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1789828, Length=254, Percent_Identity=33.8582677165354, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI87081858, Length=292, Percent_Identity=28.7671232876712, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1786599, Length=139, Percent_Identity=32.3741007194245, Blast_Score=73, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50412; Mature: 50281

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADVLVVDDEADIRDLVAGILEDEGYAVRTAADSDQALAAIRARKPALLVLDIWMQGGG
CCCCEEEECCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCEEEEEEEEECCCC
MDGLELLDMVKALDADLPVIMISGHGNIETAVSAIKRGAYEFLEKPFKSDRLLLIVERAL
CCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHH
EAAGLRRENRRLRAQTLSPDGLIGKSAPAQALRQLILKVAPANSRVLVSGPPGAGKELVA
HHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHH
RLIHGASTRARQEFVAVSAAGMAPERLDVELFGEEGEGGRPRKIGVFERAHGGTLYLDEV
HHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEECEEEECCCCEEEEHHH
ADMPRETQGRILRVLVEQRFRRVGGDNDVQVDVRVISSSSRDLRDEIAAGRFREDLFHRL
HCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHC
NVVPVRVPGLAERREDIPELINYFVERISEATGLARRRLGEDALATLQVQAWPGNVRQLR
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCHHHHHH
NNVERMLILASGEPGDVITAEMLSGAEQPSAGNAGAIGAERIIALPLREARELFEREYLN
HHHHEEEEEECCCCCCEEHHHHHCCCCCCCCCCCCCCCCHHEEECCHHHHHHHHHHHHCC
AQILRFGGNISRTANFIGMERSALHRKLKSLGVSGARGDEEE
HHHHEECCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SADVLVVDDEADIRDLVAGILEDEGYAVRTAADSDQALAAIRARKPALLVLDIWMQGGG
CCCEEEECCCCCHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCEEEEEEEEECCCC
MDGLELLDMVKALDADLPVIMISGHGNIETAVSAIKRGAYEFLEKPFKSDRLLLIVERAL
CCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHH
EAAGLRRENRRLRAQTLSPDGLIGKSAPAQALRQLILKVAPANSRVLVSGPPGAGKELVA
HHHCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHH
RLIHGASTRARQEFVAVSAAGMAPERLDVELFGEEGEGGRPRKIGVFERAHGGTLYLDEV
HHHHCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCEECEEEECCCCEEEEHHH
ADMPRETQGRILRVLVEQRFRRVGGDNDVQVDVRVISSSSRDLRDEIAAGRFREDLFHRL
HCCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHC
NVVPVRVPGLAERREDIPELINYFVERISEATGLARRRLGEDALATLQVQAWPGNVRQLR
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCCHHHHHH
NNVERMLILASGEPGDVITAEMLSGAEQPSAGNAGAIGAERIIALPLREARELFEREYLN
HHHHEEEEEECCCCCCEEHHHHHCCCCCCCCCCCCCCCCHHEEECCHHHHHHHHHHHHCC
AQILRFGGNISRTANFIGMERSALHRKLKSLGVSGARGDEEE
HHHHEECCCHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]