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The map label for this gene is ntrB [H]

Identifier: 16125984

GI number: 16125984

Start: 1912762

End: 1913853

Strand: Direct

Name: ntrB [H]

Synonym: CC_1740

Alternate gene names: 16125984

Gene position: 1912762-1913853 (Clockwise)

Preceding gene: 16125983

Following gene: 16125985

Centisome position: 47.62

GC content: 65.75

Gene sequence:

>1092_bases
GTGGCCACCGAAGCTCTGAAAGCTGCAGCTTTCGACTTGAGCCCCGAGCCTGCATTGGTCGTTGATCACGACGGCGGTCT
TGTCGCCGTGAACGAGGCCGCAGAGGCTCTGTTCGGCCACGGCCTGTCGCTTCTGGCGCGCGGGCGGTTCCGTGCAGCGC
TGCCGCCCGGGTCGGTGCTGGTGTCGCTGCTGGATCGCGCCGTGTTCGAGGGTTGCCTCGTTCGCGAGCACGGCGTCGAG
GTCAATCTCTTCGGTCAACCGCCCTTCGAGGCGGACGGCGCGGCCGTGCCACTGGGGGATGGCTCGGTTCTTCTGACCCT
GCACGTGAAGGGCGTCCTGGGCGTGGACCGCAGCGCCGATGCGGCCGGACTTCGCTCCGTCGTCGGCCTCGGCAAGATGC
TGGCCCACGAGATCAAGAACCCCTTGGCCGGCATTCGCGGCGCCGCGCAACTGTTGAAGACGGGCGCCAGCGCCGTCGAC
CAGCCCCTGGCGCAACTCATTGTCGACGAAACCGATCGGATCCGCCGTCTCGTGGATCGCATGGAGGCGTTTTCGGAGCA
GGCTCCGACCATGCGTGAGCCCGTCAATATCCACCAGGTGCTTGATCGGGTGCGCGCGCTGGTGGCCAATGGCGTCGCAG
ACGGCCTGACGCTGAAGGAAAGCTACGACCCTTCGCTGCCGCCTGTCTGGGGGGATGAGGATCACTTGATCCAGATCTTC
CTGAACCTGATGAAGAACGCCGCCGAGGCCGCCCATATGCGTGGCGACGACCGCGGTGTGCTGTCGATCCACACGGCCTG
GCGGCCCGGCGTCCGTGTCCGTGGGGCTGATGGGAAAGCCACGAGCGGCGCGCCGATCGAGGTGCGTGTGATCGATAACG
GACCGGGCGTGCCCGCCACGCTGCGCGATCATCTCTTCCAGCCGTTTGTCACCACCAAGTCGAACGGCACGGGGCTGGGT
TTGGCTTTGGTCACAAAATTAGTCACCGCCCACGGCGGTCTGATCGATTTTGAATCGGAGCCCGGCCGAACGGTCTTCCG
CGTCTTGTTGCCTGTTGCCCCTGAAGCCGCTTCCGGAGACGTCCGAGCATGA

Upstream 100 bases:

>100_bases
GCCTTGAAGATCCCGCCGCTGTGGAAAGCGCCCTCCGTACGCTTTGGCTAGCCGAGGGGAGGCAAGCCGCATGACCGACC
GCGCCCGCGTTCTGGGTGGT

Downstream 100 bases:

>100_bases
ACGCCGCGAGCAAGAAAATCCTGATCGCCGACGACGACAGCTCTATCCGTCTCGTCCTCAGTCAAGCCTTCACCCGCCTG
GGCTACCAGGTCCGCGCCAC

Product: nitrogen regulation protein NR(II)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MATEALKAAAFDLSPEPALVVDHDGGLVAVNEAAEALFGHGLSLLARGRFRAALPPGSVLVSLLDRAVFEGCLVREHGVE
VNLFGQPPFEADGAAVPLGDGSVLLTLHVKGVLGVDRSADAAGLRSVVGLGKMLAHEIKNPLAGIRGAAQLLKTGASAVD
QPLAQLIVDETDRIRRLVDRMEAFSEQAPTMREPVNIHQVLDRVRALVANGVADGLTLKESYDPSLPPVWGDEDHLIQIF
LNLMKNAAEAAHMRGDDRGVLSIHTAWRPGVRVRGADGKATSGAPIEVRVIDNGPGVPATLRDHLFQPFVTTKSNGTGLG
LALVTKLVTAHGGLIDFESEPGRTVFRVLLPVAPEAASGDVRA

Sequences:

>Translated_363_residues
MATEALKAAAFDLSPEPALVVDHDGGLVAVNEAAEALFGHGLSLLARGRFRAALPPGSVLVSLLDRAVFEGCLVREHGVE
VNLFGQPPFEADGAAVPLGDGSVLLTLHVKGVLGVDRSADAAGLRSVVGLGKMLAHEIKNPLAGIRGAAQLLKTGASAVD
QPLAQLIVDETDRIRRLVDRMEAFSEQAPTMREPVNIHQVLDRVRALVANGVADGLTLKESYDPSLPPVWGDEDHLIQIF
LNLMKNAAEAAHMRGDDRGVLSIHTAWRPGVRVRGADGKATSGAPIEVRVIDNGPGVPATLRDHLFQPFVTTKSNGTGLG
LALVTKLVTAHGGLIDFESEPGRTVFRVLLPVAPEAASGDVRA
>Mature_362_residues
ATEALKAAAFDLSPEPALVVDHDGGLVAVNEAAEALFGHGLSLLARGRFRAALPPGSVLVSLLDRAVFEGCLVREHGVEV
NLFGQPPFEADGAAVPLGDGSVLLTLHVKGVLGVDRSADAAGLRSVVGLGKMLAHEIKNPLAGIRGAAQLLKTGASAVDQ
PLAQLIVDETDRIRRLVDRMEAFSEQAPTMREPVNIHQVLDRVRALVANGVADGLTLKESYDPSLPPVWGDEDHLIQIFL
NLMKNAAEAAHMRGDDRGVLSIHTAWRPGVRVRGADGKATSGAPIEVRVIDNGPGVPATLRDHLFQPFVTTKSNGTGLGL
ALVTKLVTAHGGLIDFESEPGRTVFRVLLPVAPEAASGDVRA

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=226, Percent_Identity=42.0353982300885, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI1788549, Length=242, Percent_Identity=31.404958677686, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1790436, Length=234, Percent_Identity=32.9059829059829, Blast_Score=109, Evalue=2e-25,
Organism=Escherichia coli, GI1789149, Length=244, Percent_Identity=29.9180327868852, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI145693157, Length=234, Percent_Identity=30.3418803418803, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 38173; Mature: 38042

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATEALKAAAFDLSPEPALVVDHDGGLVAVNEAAEALFGHGLSLLARGRFRAALPPGSVL
CCCHHHHHHHCCCCCCCEEEEECCCCEEEEHHHHHHHHHCCHHHHHCCCCCCCCCHHHHH
VSLLDRAVFEGCLVREHGVEVNLFGQPPFEADGAAVPLGDGSVLLTLHVKGVLGVDRSAD
HHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEECCCCCEEEEEEEEEEECCCCCCC
AAGLRSVVGLGKMLAHEIKNPLAGIRGAAQLLKTGASAVDQPLAQLIVDETDRIRRLVDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHH
MEAFSEQAPTMREPVNIHQVLDRVRALVANGVADGLTLKESYDPSLPPVWGDEDHLIQIF
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCHHHHHHHH
LNLMKNAAEAAHMRGDDRGVLSIHTAWRPGVRVRGADGKATSGAPIEVRVIDNGPGVPAT
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCCEEEEEEECCCCCCHH
LRDHLFQPFVTTKSNGTGLGLALVTKLVTAHGGLIDFESEPGRTVFRVLLPVAPEAASGD
HHHHHHCCCEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCCCCC
VRA
CCC
>Mature Secondary Structure 
ATEALKAAAFDLSPEPALVVDHDGGLVAVNEAAEALFGHGLSLLARGRFRAALPPGSVL
CCHHHHHHHCCCCCCCEEEEECCCCEEEEHHHHHHHHHCCHHHHHCCCCCCCCCHHHHH
VSLLDRAVFEGCLVREHGVEVNLFGQPPFEADGAAVPLGDGSVLLTLHVKGVLGVDRSAD
HHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCEEECCCCCEEEEEEEEEEECCCCCCC
AAGLRSVVGLGKMLAHEIKNPLAGIRGAAQLLKTGASAVDQPLAQLIVDETDRIRRLVDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCHHHHHHHHHH
MEAFSEQAPTMREPVNIHQVLDRVRALVANGVADGLTLKESYDPSLPPVWGDEDHLIQIF
HHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCHHHHHHHH
LNLMKNAAEAAHMRGDDRGVLSIHTAWRPGVRVRGADGKATSGAPIEVRVIDNGPGVPAT
HHHHHHHHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCCEEEEEEECCCCCCHH
LRDHLFQPFVTTKSNGTGLGLALVTKLVTAHGGLIDFESEPGRTVFRVLLPVAPEAASGD
HHHHHHCCCEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHCCCCCCCCC
VRA
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430; 2881918 [H]